Protective effect of phospholipid hydroperoxide glutathione peroxidase (PHGPx) against lipid peroxidation in mussels Perna perna exposed to different metals   总被引：2，自引：0，他引：2  

de Almeida EA  Miyamoto S  Bainy AC  de Medeiros MH  Di Mascio P 《Marine pollution bulletin》2004,49(5-6):386-392

Levels of antioxidant defenses and lipid peroxidation were evaluated in mussels exposed to lead (200 mg/l), iron (500 microg/l), cadmium (200 microg/l) and copper (40 microg/l), for 12, 24, 72 and 120 h. Glutathione S-transferase (GST) activity was unchanged with all treatments. Catalase (CAT) increased after 120 h of exposure to all metals. Mussels exposed to Cd for 12 h, and to Cu and Fe for 120 h had increased lipid peroxidation, which might be associated to decreased levels of reduced glutathione (GSH) and glutathione peroxidase (GPx) activity. Pb exposure caused GSH depletion after 12 h and increased GPx activity after 120 h. Negative correlations were observed between the enzyme phospholipid hydroperoxide glutathione peroxidase (PHGPx) and malonaldehyde (MDA) levels after Fe and Cu exposure, indicating a protective role of PHGPx against lipid peroxidation, and suggesting the use of this enzyme as a new potential biomarker of toxicity associated with contaminant exposure in mussels.  相似文献   

Displacement of Orcinus orca (L.) by high amplitude sound in British Columbia, Canada   总被引：1，自引：0，他引：1  

Morton  Alexandra B.; Symonds  Helena K. 《ICES Journal of Marine Science》2002,59(1):71-80

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Effects of water-borne copper on metallothionein and lipid peroxidation in the marine amphipod gammarus locusta     

Ana D. Correia  David R. Livingstone  M. Helena Costa 《Marine environmental research》2002,54(3-5)

The aim of the current work was to determine over 10 days the effects of water-borne exposure of the marine amphipod Gammarus locusta to 4 μgCu l⁻¹ on the metallothionein (MT; measured by differential pulse polarography) protection system and lipid peroxidation (LP; thiobarbituric acid-reactive malondialdehyde equivalents) as a measure of oxidative damage. MT levels in exposed animals increased significantly at day 2 (36% > control; P<0.001) and remained high at days 6 and 10 (55 and 38%, respectively, P<0.001). The maximum level of MT at day 6 coincided with the highest Cu body-burden. LP increased within 1 day of exposure, indicative of Cu as an oxidative stressor. However, in contrast to MT, the highest LP level was seen at day 4 (68% > control, P<0.001) before returning to control values by day 6, indicating a protective role of MT against the pro-oxidant effects of Cu.  相似文献   

Holocene Storminess and Climate Change in Lofoten and Vesterålen,Northern Norway     

John A. Matthews  Helena Alexanderson 《Norsk geografisk tidsskrift. Norwegian journal of geography》2016,70(5):316-317

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Three-Dimensional Modeling of Shallow Shear-Wave Velocities for Las Vegas, Nevada, Using Sediment Type     

Barbara Luke  Helena Murvosh  Wanda Taylor  Jeff Wagoner 《中国地质大学学报(英文版)》2009,20(3)

A three-dimensional model of near-surface shear-wave velocity in the deep alluvial basin underlying the metropolitan area of Las Vegas, Nevada (USA), is being developed for earthquake site response projections. The velocity dataset, which includes 230 measurements, is interpolated across the model using depth-dependent correlations of velocity with sediment type. The sediment-type database contains more than 1 400 well and borehole logs. Sediment sequences reported in logs are assigned to one of four units. A characteristic shear-wave velocity profile b developed for each unit by analyzing closely spaced pairs of velocity profiles and well or borehole logs. The resulting velocity model exhibits reasonable values and patterns, although it does not explicitly honor the measured shear-wave velocity profiles. Site response investigations that applied a preliminary version of the velocity model support a two-zone ground-shaking hazard model for the valley. Areas in which clay predominates in the upper 30 m are predicted to have stronger ground motions than the rest of the basin.  相似文献   

The polycyclic aromatic hydrocarbon concentrations in soils in the Region of Valasske Mezirici, the Czech Republic     

Daniela Plachá  Helena Raclavská  Dalibor Matýsek  Mark H Rümmeli 《Geochemical transactions》2009,10(1):12-21

The polycyclic aromatic hydrocarbon (PAH) contamination of urban, agricultural and forest soil samples was investigated from samples obtained in the surroundings of Valasske Mezirici. Valasske Mezirici is a town located in the north-east mountainous part of the Czech Republic, where a coal tar refinery is situated. 16 PAHs listed in the US EPA were investigated. Organic oxidizable carbon was also observed in the forest soils. The PAH concentrations ranged from 0.86-10.84 (with one anomalous value of 35.14) and 7.66-79.39 mg/kg dm in the urban/agricultural and forest soils, respectively. While the PAH levels in the urban/agricultural soils are within the range typically found in industrialized areas, the forest soils showed elevated PAH concentrations compared to other forest soils in Western and Northern Europe. The PAH concentrations and their molecular distribution ratios were studied as functions of the sample location and the meteorological history. The soils from localities at higher altitudes above sea level have the highest PAH concentrations, and the PAH concentrations decrease with increasing distance from the town.  相似文献   

A multi-method approach to dating middle and late Quaternary high relative sea-level events on NW Svalbard – A case study     

Helena Alexanderson  Jon Y. Landvik  Anatoly Molodkov  Andrew S. Murray 《Quaternary Geochronology》2011,6(3-4):326-340

Waxing and waning ice sheets and changing sea levels have been interpreted from the Quaternary stratigraphic record at Leinstranda, Brøggerhalvøya in NW Svalbard. We have identified seven high relative sea-level events, related to glacio-isostatic loading, and separated by at least four glacial events. To establish a chronology for the high sea-level events (interstadials and interglacials) and the intervening glaciations, we have used three different absolute dating methods: optically stimulated luminescence (OSL) of shallow marine deposits, and electron spin resonance (ESR) and radiocarbon (AMS-¹⁴C) dating of fossils contained in these sediments. Of the absolute dating methods, OSL has provided the stratigraphically most consistent dataset and which also matches a biostratigraphically inferred interglacial. The ESR ages of mollusc shells suffer from low precision due to unusually large uranium content in most dated shell samples, which in turn is most likely a result of significant recent uranium enrichment of the sediments. Most radiocarbon ages are non-finite. The results show that the high relative sea-level events range in age from the Saalian sensu lato (≥Marine Isotope Stage, MIS, 6) to the early Holocene (MIS 1), and include events OSL-dated to 185 ± 8 ka, 129 ± 10 ka, 99 ± 8 ka and 36 ± 3 ka. The methods used by us and by previous investigators of the same site are compared and assessed, and sources of error, accuracy and precision of ages are discussed.  相似文献   

Fatty acid profiles indicate the habitat of mud snails Hydrobia ulvae within the same estuary: Mudflats vs. seagrass meadows     

Helena Coelho  Teresa Lopes da Silva  Alberto Reis  Henrique Queiroga  João Serôdio  Ricardo Calado 《Estuarine, Coastal and Shelf Science》2011

Mud snails Hydrobia ulvae occupy different habitats in complex estuarine ecosystems. In order to determine if fatty acid profiles displayed by mud snails can be used to identify the habitat that they occupy within the same estuary, fatty acids of H. ulvae from one mudflat and one seagrass meadow in the Ria de Aveiro (Portugal) were analyzed and compared to those displayed by microphytobenthos (MPB), the green leaves (epiphyte-free) of Zostera noltii, as well as those exhibited by the epiphytic community colonizing this seagrass. MPB and epiphytic diatom-dominated samples displayed characteristic fatty acids, such as 16:1n-7 and 20:5n-3, while 18:2n-6 and 18:3n-3 were the dominant fatty acids in the green leaves of Z. noltii. Significant differences between the fatty acid profiles of H. ulvae specimens sampled in the mudflat and the seagrass meadow could be identified, with those from the mudflat displaying higher levels of fatty acids known to be characteristic of MPB. This result points towards the well known existence of grazing activity on MPB by mud snails. The fatty acid profiles displayed by H. ulvae inhabiting the seagrass meadows show no evidence of direct bioaccumulation of the two most abundant polyunsaturated fatty acids of Z. noltii (18:2n-6 and 18:3n-3) in the mud snails, which probably indicates that either these compounds can be metabolized to produce energy, used as precursors for the synthesis of essential fatty acids, or that the snails do not consume seagrass leaves at all. Moreover, the fatty acid profiles of mud snails inhabiting the seagrass meadows revealed the existence of substantial inputs from microalgae, suggesting that the epiphytic community colonizing the leaves of Z. noltii displays an important role on the diet of these organisms. This assumption is supported by the high levels of 20:5n-3 and 22:6n-3 recorded in mud snails sampled from seagrass meadows. In conclusion, fatty acid analyses of H. ulvae can be successfully used to identify the habitat occupied by these organisms within the same estuary (e.g. mudflats and seagrass meadows) and reveal the existence of contrasting dietary regimes.  相似文献   

Nutrient limitation of phytoplankton growth in the freshwater tidal zone of a turbid,Mediterranean estuary     

Rita B. Domingues  Tânia P. Anselmo  Ana B. Barbosa  Ulrich Sommer  Helena M. Galvão 《Estuarine, Coastal and Shelf Science》2011

Identification of the limiting nutrient(s) is a requirement for the rational management of eutrophication. Here, we present the first experimental analysis of nutrient limitation of phytoplankton growth and its seasonal variation in the Guadiana estuary (SE Portugal-SW Spain). Ten microcosm experiments were performed during 2005 and 2008, using water samples collected in the freshwater tidal zone of the Guadiana estuary. Nitrate, phosphate and silicate were added in a single pulse, alone and in combinations. Experimental treatments were incubated for 4 days under controlled laboratory conditions. Phytoplankton response to nutrient enrichment was evaluated through changes in biomass (Chla), and abundance of specific phytoplankton groups.  相似文献   

Weather maps classification over Greek domain based on isobaric line patterns     

Athanassios Zagouras  Athanassios A. Argiriou  George Economou  Spiros Fotopoulos  Helena A. Flocas 《Theoretical and Applied Climatology》2013,114(3-4):691-704

The paper presents a semi-supervised weather classification method based on 850-hPa isobaric level maps. A preprocessing step is employed, where isolines of geopotential height are extracted from weather map images via an image processing procedure. Α feature extraction stage follows where two techniques are applied. The first technique implements phase space reconstruction, and yields multidimensional delay distributions. The second technique is based on chain code representation of signals, from which histogram features are derived. Similarity measures are used to compare multidimensional data and the k-means algorithm is applied in the final stage. The method is applied over the area of Greece, and the resulting catalogues are compared to a subjective classification for this area. Numerical experiments with datasets derived from the European Meteorological Bulletin archives exhibit an up to 91 % accurate agreement with the subjective weather patterns.  相似文献