Generation of reactive oxygen species in different fractions of the coelomocytes of holothurian Eupentacta fraudatrix in response to the thermostable toxin of Yersinia pseudotuberculosis in vitro     

Dolmatova L. S.  Eliseykina M. G.  Timchenko N. F.  Kovaleva A. L.  Shitkova O. A. 《中国海洋湖沼学报》2003,21(4):293-304

Pure fraction (92%–95%) of phagocytes (FP) and a mixture of amoebocytes (62%) and morula cells (38%)-FPMC- of the holothurianEupentacta fraudatrix' (Holothuroidea, Dendrochirota) were obtained by using ficoll-verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin ofYersinia pseudotuberculosis (TST) at different concentrations (0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration-dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 h treatment, and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5-fold in FPMC compared to the control. With addition of TST, lectin stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously, ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians. This work was financially supported by the Russian Foundation for Fundamental Research Grant (No. 00-04-48949).  相似文献   

Planning for Air Pollution Abatement in the Greater Tehran Area (GTA) in the Islamic Republic of Iran (JICA Project 1995–1997)     

O. Yokoyama  K. Takahashi 《Pure and Applied Geophysics》2003,160(1-2):17-19

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Results from several persistent train spectra intercompared     

J. Rajchl  J. Bočcek  D. Očenáš  J. Škvarka  P. Zimnikoval  H. Murayama  K. Ohtsuka 《Earth, Moon, and Planets》1995,68(1-3):479-486

Some results following from two contemporary photographical programs (Slovakia and Japan) for persistent meteor train spectra are compared. It shows that even though both programs are not too different according to their lenses and films used, the spectral interval detected is very different. In this respect prism as a dispersion element is more favourable than the grating with blaze wavelength near 610 nm. However, some results previously assumed as typicai for presented persistent trains-e.g. the forbidden Herzberg O₂ emission, the NO₂ continuum and OH red emissions-seem to be substanciated especially due to higher and linear dispersion of those grating spectra.  相似文献   

XMM–Newton observations of three short-period polars: V347 Pav, GG Leo and EU UMa     

Gavin Ramsay  Mark Cropper  K. O. Mason  F. A. Córdova  W. Priedhorsky 《Monthly notices of the Royal Astronomical Society》2004,347(1):95-100

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The evolutionary status of the white dwarf companion of the binary pulsar PSR J1713+0747     

O. G. Benvenuto †  R. D. Rohrmann ‡  M. A. De Vito § 《Monthly notices of the Royal Astronomical Society》2006,366(4):1520-1528

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Radiative association in Li+H revisited: the role of quasi-bound states     

O. J. Bennett  A. S. Dickinson  T. Leininger  F. X. Gadéa 《Monthly notices of the Royal Astronomical Society》2003,341(1):361-368

The role in radiative association of narrow resonances arising from quasi-bound levels is examined. For the rate coefficient the contribution from such levels is readily calculated in terms of the Einstein A -coefficient from bound rovibrational levels generated using a suitable alternative boundary condition. By locating systematically all the quasi-bound levels, an enhancement by more than an order of magnitude of the rate coefficient for Li(2s) + H(1s) association above about 600 K is obtained. The effect on the LiH abundance in the early Universe is no more than an increase of a factor of 3 for limited ranges of values of the redshift. For the Li(2p) state the effect of narrow resonances appears to have been taken into account in previous calculations.  相似文献   

Ice‐cover variability on shallow lakes at high latitudes: model simulations and observations     

Claude R. Duguay  Greg M. Flato  Martin O. Jeffries  Patrick Mnard  Kim Morris  Wayne R. Rouse 《水文研究》2003,17(17):3465-3483

A one‐dimensional thermodynamic model for simulating lake‐ice phenology is presented and evaluated. The model can be driven with observed daily or hourly atmospheric forcing of air temperature, relative humidity, wind speed, cloud amount and snowfall. In addition to computing the energy balance components, key model output includes the temperature profile at an arbitrary number of levels within the ice/snow (or the water temperature if there is no ice) and ice thickness (clear ice and snow‐ice) on a daily basis, as well as freeze‐up and break‐up dates. The lake‐ice model is used to simulate ice‐growth processes on shallow lakes in arctic, sub‐arctic, and high‐boreal forest environments. Model output is compared with field and remote sensing observations gathered over several ice seasons. Simulated ice thickness, including snow‐ice formation, compares favourably with field measurements. Ice‐on and ice‐off dates are also well simulated when compared with field and satellite observations, with a mean absolute difference of 2 days. Model simulations and observations illustrate the key role that snow cover plays on the seasonal evolution of ice thickness and the timing of spring break‐up. It is also shown that lake morphometry, depth in particular, is a determinant of ice‐off dates for shallow lakes at high latitudes. Copyright © 2003 John Wiley & Sons, Ltd.  相似文献   

Baryon loading and the Weibel instability in gamma-ray bursts     

M. Fiore  L. O. Silva  C. Ren  M. A. Tzoufras  W. B. Mori 《Monthly notices of the Royal Astronomical Society》2006,372(4):1851-1855

The dynamics of two counter-streaming electron–positron–ion unmagnetized plasma shells with zero net charge is analysed in the context of magnetic field generation in gamma-ray burst internal shocks due to the Weibel instability. The effects of large thermal motion of plasma particles, arbitrary mixture of plasma species and space charge effects are taken into account. We show that, although thermal effects slow down the instability, baryon loading leads to a non-negligible growth rate even for large temperatures and different shell velocities, thus guaranteeing the robustness and the occurrence of the Weibel instability for a wide range of scenarios.  相似文献   

Identification of a 12–17 d time-scale in X-ray observations of GRS 1915+105     

J. Greenhough  S. C. Chapman  S. Chaty  †  R. O. Dendy  G. Rowlands 《Monthly notices of the Royal Astronomical Society》2003,340(3):851-855

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The ELAIS deep X-ray survey – I. Chandra source catalogue and first results     

J. C. Manners †  O. Johnson  O. Almaini  C. J. Willott  E. Gonzalez-Solares  A. Lawrence  R. G. Mann  I. Perez-Fournon  J. S. Dunlop  R. G. McMahon  S. J. Oliver  M. Rowan-Robinson  S. Serjeant 《Monthly notices of the Royal Astronomical Society》2003,343(1):293-305

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