Cloning and sequence analysis of a full-length cDNA of <Emphasis Type="Italic">SmPP1cb</Emphasis> encoding turbot protein phosphatase 1 beta catalytic subunit     

亓飞  郭华荣  王建 《中国海洋湖沼学报》2008,26(1):54-61

Reversible protein phosphorylation, catalyzed by protein kinases and phosphatases, is an important and versatile mechanism by which eukaryotic cells regulate almost all the signaling processes. Protein phosphatase 1 （PP1） is the first and well-characterized member of the protein serine/threonine phosphatase family. In the present study, a full-length cDNA encoding the beta isoform of the catalytic subunit of protein phosphatase l（PPlcb）, was for the first time isolated and sequenced from the skin tissue of flatfish turbot Scophthalmus maximus, designated SmPPlcb, by the rapid amplification of cDNA ends （RACE） technique. The cDNA sequence of SmPPlcb we obtained contains a 984 bp open reading frame （ORF）, flanked by a complete 39 bp 5＇ untranslated region and 462 bp 3＇ untranslated region. The ORF encodes a putative 327 amino acid protein, and the N-terminal section of this protein is highly acidic, Met-Ala-Glu-Gly-Glu-Leu-Asp-Val-Asp, a common feature for PP1 catalytic subunit but absent in protein phosphatase 2B （PP2B）. And its calculated molecular mass is 37 193 Da and pI 5.8. Sequence analysis indicated that, SmPPlcb is extremely conserved in both amino acid and nucleotide acid levels compared with the PPlcb of other vertebrates and invertebrates, and its Kozak motif contained in the 5＇UTR around ATG start codon is GXXAXXGXXATGG, which is different from mammalian in two positions A6 and G3, indicating the possibility of different initiation of translation in turbot, and also the 3＇UTR of SmPPlcb is highly diverse in the sequence similarity and length compared with other animals, especially zebraf＇lsh. The cloning and sequencing of SmPPlcb gene lays a good foundation for the future work on the biological functions of PP1 in the flatfish turbot.  相似文献   

Tidal current observation in the southern Yellow Sea in the summers of 2001 and 2003   总被引：1，自引：0，他引：1  

刘志亮  胡敦欣  唐晓晖 《中国海洋湖沼学报》2008,26(2):121-129

Direct current observations in the Yellow Sea interior are very scarce due to intense fishing and trawling activities. Most previous studies on tides in the area were based on coastal measurements or satellite altimeter sea levels and have not been rigorously compared with direct measurements. In this paper, tidal currents are studied with current profiles from three bottom-moored Sontek Acoustic Doppler Profilers （ADPs） deployed in the southern Yellow Sea in summer of 2001 and 2003. The measured current series were dominated by tidal currents. Maximum velocities are between 40-80 cm/s at the mooring stations. M2 current is the most dominant primary tidal constituent, while MS4 and M4 are the most significant shallow water tides with much smaller amplitudes than the primary tides.  相似文献   

Rossby waves with linear topography in barotropic fluids   总被引：1，自引：0，他引：1  

杨联贵  达朝究  宋建  张会琴  杨红丽  侯一筠 《中国海洋湖沼学报》2008,26(3):334-338

Rossby waves are the most important waves in the atmosphere and ocean, and are parts of a large-scale system in fluid. The theory and observation show that, they satisfy quasi-geostrophic and quasi-static equilibrium approximations. In this paper, solitary Rossby waves induced by linear topography in barotropic fluids with a shear flow are studied. In order to simplify the problem, the topography is taken as a linear function of latitude variable y, then employing a weakly nonlinear method and a perturbation method, a KdV （Korteweg-de Vries） equation describing evolution of the amplitude of solitary Rossby waves induced by linear topography is derived. The results show that the variation of linear topography can induce the solitary Rossby waves in barotropic fluids with a shear flow, and extend the classical geophysical theory of fluid dynamics.  相似文献   

Molecular phylogenetic analysis of attached Ulvaceae species and free-floating <Emphasis Type="Italic">Enteromorpha</Emphasis> from Qingdao coasts in 2007     

姜鹏  王金锋  崔玉琳  李友训  林瀚智  秦松 《中国海洋湖沼学报》2008,26(3):276-279

Based on the sequence data of the nuclear ribosomal DNA internal transcribed spacer (ITS) 1, 5.8 S, and ITS 2, the molecular phylogeny was analyzed on Ulvaceae species collected from Qingdao coasts in summer of 2007, including 15 attached Ulva and Enteromorpha samples from 10 locations and 10 free-floating Enteromorpha samples from seven locations. The result supported the monophyly of all free-floating Enteromorpha samples, implying the unialgal composition of the free-floating Enteromorpha, and the attached Ulvaceae species from Qingdao coasts were grouped into other five clades, suggesting that they were not the biogeographic origin of the free-floating Enteromorpha in that season. Supported by NSFC (40506030); the Innovative Key Project of the Chinese Academy of Sciences (KZCX2-YW-209); Science & Technology Project of Qingdao City (06-2-2-12-JCH)  相似文献   

Isotopic tracing of ore-forming source materials for Dexing porphyry copper deposit of Jiangxi, China     

References： 《东北亚地学研究》2008,11(1):25-31

Dexing copper deposit is the biggest porphyry copper deposit in China. By researching isotopes of C, Si and Cu from the samples of Tongchang and Fujiawu ore-field, the authors found that δ^13 CpDB values of siderite were close to the δ^13 CpDB value of original magma; δ^30Si values of the samples at the ore-forming stage were close to the δ^30 Si value range of magma, δ^30Si values of partial samples were far away from it; Cu isotopic compositions of massive chalcopyrite formed at the early ore-forming stage are higher than that of veinal chalcopyrite formed at the later ore-forming stage. The results show that ore-forming materials were mainly derived from the porphyry body, and part of them were from wall rock materials.  相似文献   

面向汉语的自然语言路径描述方法   总被引：2，自引：1，他引：1  

张雪英  闾国年  宦建 《地球信息科学学报》2008,10(6):757-762

自然语言路径描述是运用日常生活中人们习惯的语言方式和思维方式来表达路径信息的。而可视化描述和自然语言描述相结合,可以使电子地图向用户提供图文并茂的路径查询服务。目前,电子地图在移动设备和Internet日益普及,自然语言路径描述成为电子地图大众化和人性化服务的重要标志之一。因此,路径描述中需要重点解决空间关系的自然语言描述问题。自然语言中空间关系描述的语言特征与心理学和认知语言学有着非常密切的关系。本文描述了针对汉语的路径描述词汇库的结构与内容,并根据汉语特点设计了路径描述的单元和复合句法模式,以解决词汇受限和句法模式单一的问题,提出了道路名称、沿途标志性地物和方向判断等路径描述补充信息的获取方法,以增加路径描述的可理解性。并以南京市电子地图为例,开发了电子地图查询原型系统。该系统能够在地图上可视化显示最短路径的同时,自动生成该路径的自然语言描述。  相似文献   

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唐卫明  孙红星  陈江 《大地测量与地球动力学》2008,28(5):68-72

??LAMBDA????????GNSS????????????????????????????????????????????????????????????????????????Ratio??????????????????Ratio?????????????????????L1??L2?????λ????????????????????÷?????????????????????????????????????????????????????????  相似文献   

SIG中空间信息可视化与互操作实现研究   总被引：1，自引：0，他引：1  

黄方  刘定生  李国庆  王建  于文洋  赵灵军 《地理信息世界》2008,6(3)

在介绍SIG示范验证平台总体架构的基础上,提出了WMS服务实体在该架构下的有效布局、层次结构、服务模型、工作流程等;并介绍了利用开源软件MapServer构建这类WMS服务实体的关键技术.通过WMS服务实体在SIG示范验证平台中的应用实例与效率分析,表明该实现途径与技术是切实可行的.  相似文献   

Sequence analysis and quantitative detection of Norwalk-like viruses in cultured oysters of China     

Jun Wang  Qingjuan Tang  Zhiqin Yue  Zhaojie Li  Jin Zhang  Changhu Xue 《中国海洋大学学报(英文版)》2008,7(2):223-227

We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future.  相似文献   

基于星敏感器的航天器姿态估计     

王正亮  唐争气  安德笼 《现代测绘》2009,32(1)

星敏感器是一种高精度的姿态测量仪器,本文在分析星敏感器姿态测量原理的基础上,利用序贯平差方法确定航天器姿态并予以实现.仿真结果表明,该算法能满足实时姿态确定的精度要求,且降低了数据存储量,节省了存储单元,具有较强的灵活性.  相似文献