Maternal transfer of bioactive polychlorinated aromatic hydrocarbons in spawning chinook salmon (Oncorhynchus tschawytscha)     

Gerald T. Ankley  Donald E. Tillitt  John P. Giesy 《Marine environmental research》1989,28(1-4)

The biological potency (relative to 2,3,7,8-tetrachlorodibenzo-p-dioxin. TCDD) of planar polychlorinated aromatic hydrocarbons (PCHs) in extracts of eggs and flesh from spawning female chinook salmon (Oncorhynchus tschawytscha) from Lake Michigan was determined by measuring the induction of 7-ethoxyresorufin O-deethylase activity in H-4-II-E rat hepatoma cells. TCDD-equivalents in flesh and egg samples ranged from 0 to 115·8 pg/g, and were approximately 5-fold greater in eggs than in flesh. These results suggest that the maternal transfer of PCHs may play a role in determining the reproductive success of Lake Michigan chinook salmon.  相似文献   

Transient soil response in a porous seabed with variable permeability     

Brian R. Seymour  Dong S. Jeng  John R.C. Hsu 《Ocean Engineering》1996,23(1):27-46

The soil permeability of many natural marine sediments decreases with depth because of consolidation under overburden pressure. This is accompanied by a decrease in porosity and void ratio that also affect the permeability. Conventional theories for wave-induced soil response have assumed a homogeneous porous seabed. This paper presents a new approach for the wave-induced response in a soil matrix, with variable permeability as a function of burial depth. The soil matrix considered is unsaturated and anisotropic, and is subject to a three-dimensional wave system. The pore pressure and effective stresses induced by such a system are obtained from a set of equations incorporating a variable permeability. Verification is available through reduction to the simple case of uniform permeability. The results indicate that the effect of variable soil permeability on pore pressure and vertical effective stress may be significant, especially in a gravelled seabed and for unsaturated sandy soils.  相似文献   

Government response to marine pollution from ships     

John Bywater  Commander 《Marine Policy》1995,19(6)

The Marine Pollution Control Unit (MPCU) is the competent national authority for discharging the Secretary of State for Transport responsibility for pollution from ships under the Prevention of Oil Pollution Act 1971 and subsequent legislation covering other hazardous substances. It is an integral part of The Coastguard Agency, an executive agency of The Department of Transport. MPCU is responsible for maintaining the National Contingency Plan, for monitoring and assessing all pollution incidents within UK waters including dealing with pollution from ships and for advising on/co-ordinating shoreline counter pollution operations, and for fostering and maintaining international relationships for mutual support.  相似文献   

Effects of organic pollutants on methanogenesis,sulfate reduction and carbon dioxide evolution in salt marsh sediments     

Ronald P. Kiene  Douglas G. Capone 《Marine environmental research》1984,13(2):141-160

Anaerobic salt marsh sediments were amended with a variety of organic pollutants and the effects on methanogenesis, sulfate reduction and carbon dioxide evolution were examined. Addition of 1000 μg g^?1 (dry weight sediment) Arochlor 1221, lindane, endrin, benzene and phenanthrene resulted in no significant effects on the activities studied. Methanogenesis was inhibited by 1000 μg g^?1toxaphene, PCP, chlordane, naphthalene, DDT, Kepone and heptachlor and by 100 μg g^?1 PCP and toxaphene. At 1000 μg g^?1 naphthalene and toxaphene and 100 μg g^?1 PCP, a period of initial inhibition of methanogenesis was followed by stimulation relative to controls. Arochlor 1254 (1000 μg g^?1) and Temik (500 and 10 μg g^?1) stimulated methanogenesis from the outset. Temik at 500 μg g^?1 gave the greatest stimulation of methanogenesis (900% of controls) of any of the compounds studied. Sulfate reduction was inhibited by 1000 μg g^?1 PCP, toxaphene, naphthalene and chlordane and by 500 μg g^?1 atrazine and 100 μg g^?1 heptachlor. Sustained inhibition of sulfate reduction by naphthalene, toxaphene and PCP may have contributed to the stimulation of methanogenesis. Carbon dioxide evolution was not significantly affected by most of the compounds studied except for 100 μg g^?1 PCP and 1000 μg g^?1 aphthalene, each of which gave significant inhibition in only one of three experiments.Concentrations of individual organic pollutants required to cause observable effects were high. It is concluded that, except for highly polluted sediments, methanogenesis, sulfate reduction and CO₂ evolution would not be affected by the compounds studied here at concentrations typically found in the environment.  相似文献   

CO₂ cycling in the coastal ocean. I – A numerical analysis of the southeastern Bering Sea with applications to the Chukchi Sea and the northern Gulf of Mexico     

John J. Walsh  Dwight A. Dieterle 《Progress in Oceanography》1994,34(4)

A quasi-two dimensional model of the carbon and nitrogen cycling above the 70m isobath of the southeastern Bering Sea at 57°N replicates the observed seasonal cycles of nitrate, ammonium, ΣCO₂, pCO₂, light penetration, chlorophyll, phytoplankton growth rate, and primary production, as constrained by changes in wind, incident radiation, temperature, ice cover, vertical and lateral mixing, grazing stress, benthic processing of phytodetritus and zooplankton fecal pellets, and the pelagic microbial loop of DOC, bacteria, and their predators. About half of the seasonal resupply of nitrate stocks to their initial winter conditions is derived from in situ nitrification, with the rest obtained from deep-sea influxes. Under the present conditions of atmospheric forcing, shelf-break exchange, and food web structure, this shelf ecosystem serves as a sink for atmospheric CO₂, with storage in the forms of exported DOC, DIC, and unutilized POC (phytoplankton, bacteria, and fecal pellets).As a consequence of just the rising levels of atmospheric pCO₂ since the the Industrial Revolution, however, the biophysical CO₂ status of the Southeastern Bering Sea shelf may have switched over the last 250 years, from a prior source to the present sink, since this relatively pristine ecosystem has unergone little eutrophication. Such fluctuations of CO₂ status may thus be reversed by the physical processes of : (1) reduction of atmospheric pCO₂, (2) increased on welling of deep-sea ΣCO₂, and (3) warming of shelf waters. Based on our application of this model to the Chukchi Sea and the Gulf of Mexico, about 1.0–1.2 gigatons C y^-1 of atmospheric CO₂ may now be sequestered by temperate and polar shelf ecosystems. When tropical systems are included, however, a positive net sink of only 0.6–0.8. × 10¹⁵g C y⁻¹ may prevail over all shelves.  相似文献   

Threshold of motion of coarse-grained sediment under waves in shallow water   总被引：1，自引：0，他引：1  

John C. Morfett   《Coastal Engineering》1992,18(3-4)

This paper considers the threshold of motion of sediment in shallow coastal waters under breaking and non-breaking waves. A simple model, representing conditions at the fluid/sediment interface, is developed. The representation of a breaking wave is based on bore theory, and the shear stress at the bed is based on the friction formula of O'Connor and Yoo. A threshold formula is presented based on the use of energy dissipation. The threshold data is also related to the Shields threshold criterion.  相似文献   

The distribution of tritium and CFCs in the Weddell Sea during the mid-1980s     

Manfred Mensch  Reinhold Bayer  John L. Bullister  Peter Schlosser  Ray F. Weiss 《Progress in Oceanography》1996,38(4):377-388

Transient tracer data (tritium, CFC11 and CFC12) from the southern, central and northwestern Weddell Sea collected during Polarstern cruises ANT III-3, ANT V-2/3/4 and during Andenes cruise NARE 85 are presented and discussed in the context of hydrographic observations. A kinematic, time-dependent, multi-box model is used to estimate mean residence times and formation rates of several water masses observed in the Weddell Sea.Ice Shelf Water is marked by higher tritium and lower CFC concentrations compared to surface waters. The tracer signature of Ice Shelf Water can only be explained by assuming that its source water mass, Western Shelf Water, has characteristics different from those of surface waters. Using the transient nature of tritium and the CFCs, the mean residence time of Western Shelf Water on the shelf is estimated to be approximately 5 years. Ice Shelf Water is renewed on a time scale of about 14 years from Western Shelf Water by interaction of this water mass with glacial ice underneath the Filchner-Ronne Ice shelf. The Ice Shelf Water signature can be traced across the sill of the Filchner Depression and down the continental slope of the southern Weddell Sea. On the continental slope, new Weddell Sea Bottom Water is formed by entrainment of Weddell Deep Water and Weddell Sea Deep Water into the Ice Shelf Water plume. In the northwestern Weddell Sea, new Weddell Sea Bottom Water is observed in two narrow, deep boundary currents flowing along the base of the continental slope. Classically defined Weddell Sea Bottom Water (θ ≤ −0.7°C) and Weddell Sea Deep Water (−0.7°C ≤ θ ≤ 0°C) are ventilated from the deeper of these boundary currents by lateral spreading and mixing. Model-based estimates yield a total formation rate of 3.5Sv for new Weddell Sea Bottom Water (θ = −1.0°C) and a formation rate of at least 11Sv for Antarctic Bottom Water (θ = −0.5°C).  相似文献   

Photoadaptation of carboxylating enzymes and photosynthesis during a spring bloom     

John Clegg Smith  Trevor Platt  William G. Harrison 《Progress in Oceanography》1983,12(4):425-459

Properties of the light saturation curve of photosynthesis and ribulose-1,5-bisphosphate carboxylase (RuBPC) activity are shown to change qualitatively in a natural population of marine phytoplankton during a spring bloom. Evidence is presented to show that these changes constitute photoadapative responses to increasing irradiance. As irradiance increased during the bloom, both the level of light-saturated photosynthesis (P_m) and the initial slope of the light saturation curve (α = photosynthetic efficiency) increased whether those parameters were normalized to chlorophyll a concentration (P_m^b, α^b) or to cell numbers (P_m^c, α^c). The magnitudes of these changes were such that I_k (= P_m/α, the photoadaptation parameter) did not change, but I_m, the light intensity at which photosynthesis becomes saturated, increased. RuBPC activity, both chlorophyll a (RuBPC^b) and cell number normalized (RuBPC^c), also increased during the bloom. We suggest that these adaptations were achieved by simultaneously increasing the number of photosynthetic units, proportionately decreasing the photosynthetic unit size, and increasing both the concentrations of the enzymes of the dark reactions and possibly also of photosynthetic electron transport components.We also observed diminished levels of photoinhibition in the high light adapted cells late in the bloom and have suggested that this was a consequence of the same suite of physiological changes.In situ carbon fixation per cell increased during the bloom whereas no change occurred in this parameter when normalized to chlorophyll a concentration. Although these photoadaptive responses thus permitted carbon to be fixed in situ more rapidly per cell, at a constant efficiency with respect to investment of energy in the photosynthetic apparatus, they did not result in a change in growth rate. Based on consideratios of the role of time scale in physiological adaptation, however, it is suggested that the observed alterations in photosynthesis with increasing irradiance might permit a cell to more rapidly fill an energy quota for division, possibly an advantage in a mixing environment in which energy is patchily distributed, both spatially and temporalyy.Phosphoenolpyruvate carboxylase activity when normalized to chlorophyll a (PEPC^b) did not change during the bloom while chlorophyll a normalized dark carbon fixation decreased sharply and was quantitatively small compared to PEPC^b. On this basis and considering that RuBPC^b increased during the bloom, it is suggested that, although PEPC may be involved in dark carbon fixation, its most important quantitative role is probably an indirect one in light dependent photosynthesis.We have also considered the relevance of laboratory results on photoadaptation to interpretations of field studies and have suggested that batch culture studies must be treated with caution but that turbidistat and semi-continuous methods provide reasonable simulations of natural conditions.  相似文献   

Simultaneous exposure of English sole (Parophrys vetulus) to sediment-associated xenobiotics: Part 1—uptake and disposition of ¹⁴C-polychlorinated biphenyls and ³H-benzo[a]pyrene     

John E. Stein  Tom Hom  Usha Varanasi 《Marine environmental research》1984,13(2):97-119

English sole (Parophrys vetulus) were exposed to environmentally realistic levels of sediment-associated ³H-benzo[a]pyrene (3 μg BaP/g sediment, dry weight) and ¹⁴C-Aroclor 1254 (1 μg PCBs/g), separately and together, for up to 10 days. BaP and its metabolites in tissues reached steady-state concentrations by the first day of the exposure, whereas PCBs did not approach steady-state concentrations until the tenth day of exposure. Simultaneous exposure of sole to BaP and PCBs, relative to separate exposure to the xenobiotics, significantly increased the concentrations of BaP-derived radioactivity and decreased the concentrations of PCB-derived radioactivity in some tissues and bile. Accumulation of PCB-derived radioactivity, estimated as the burden in tissues, was significantly greater (4- to 13-fold) than that of BaP-derived radioactivity throughout the experiments and regardless of the type of exposure. The rank of the concentrations of PCB-derived radioactivity in tissues and bile was: bile ～ liver > brain > kidney ～ gill > skin ～ blood ～ muscle and for BaP-derived radioactivity the order was: bile > liver > gill > kidney > skin ～ blood > muscle > brain. BaP-derived radioactivity in liver and bile was present primary (85–99%) as metabolites, whereas PCB-derived radioactivity was present equally as parent compounds and metabolites in bile and primarily (98 %) as parent compounds in liver. Hydrolysis of bile from PCB/BaP-exposed sole with β-glucuronidase/arylsulfatase released 35 % of the BaP-derived radioactivity and 32 % of the PCB-derived radioactivity as primary metabolites. A much higher proportion of the BaP-derived radioactivity (64 %) than the PCB-derived radioactivity (13 %) in bile was unaffected by the enzyme treatment, indicating differences in the conjugation of the primary metabolites of PCBs and BaP. The results suggest that in contaminated environments the tissue to sediment concentration ratios for PCBs would exceed that for BaP and its metabolites; however, BaP would be continually absorbed and metabolized by sole to potentially carcinogenic and mutagenic compounds. In addition, the tissue levels of these toxic compounds may be increased by simultaneous exposure to PCBs.  相似文献   

A fish hepatocyte model for the investigation of the effects of environmental contaminants     

Sandra M. Baksi  John M. Frazier 《Marine environmental research》1988,24(1-4)

An isolated fish hepatocyte culture system was developed as a models ystem to investigate the mechanisms of action of environmental contaminants. Hepatocytes were isolated from striped bass (Morone saxatilis) by an adaptation of the two stage perfusion technique of Seglen.¹ The system was used to evaluate metal binding protein (MBP) induction in response to cadmium, a primary inducer of metallothionein (MT) in rat hepatocytes. Striped bass hepatocytes appeared to be refractory to the induction of MBP by cadmium, since there was no significant increase in the synthesis of MBP for any of the doses at any of the time points investigated. However, when a similar experiment was performed using rat hepatocytes there was induction of MBP that was related to both dose and time. These comparative experiments indicate that although there are similarities between the hepatocytes of the two species in regard to ³⁵S incorporation into low molecular weight metal-binding proteins, there appear to be significant quantitative differences as well in regard to MBP kinetics. This in vitro model system could potentially be utilized to investigate the toxicological properties of other environmental contaminants.  相似文献