CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES     

郭华荣  张士璀  李红岩  童裳亮  相建海 《中国海洋湖沼学报》2002,20(3):226-231

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.  相似文献   

Technique for Controlling Spread of Limnotic Oncomelania   总被引：1，自引：0，他引：1  

李大美  王祥三  LAI Yonggen 《中国海洋湖沼学报》2003,21(3):251-260

Schistosomiasis is a parasitic disease mostly found in areas along the Changjiang River of China. The disease is spread solely through an intermediary named oncomelania, so its spread of schistosomiasis can be controlled by properly designing water intakes which prevent oncomelania from entering farming land or residential areas. This paper reports a successful design process and a new oncomelania-free intake device. The design of the new intake is based on a sound research program in which extensive experimental studies were carried out to gain knowledge of oncomelania eco-hydraulic behavior and detailed flow field information through CFD simulation.  相似文献   

未来的国际天球参考架     

金文敬  李东明  唐正宏  王叔和 《紫金山天文台台刊》2003,(1)

本文简述了国际天球参考架的发展历史和现在射电参考架的现状—基准源选择的标准和参考架的稳定性。描述了地面上光学观测在依巴谷参考架的维持和加密的一系列工作。介绍由天体测量卫星GAIA和SIM给出的天球参考架可能逵到的精度。详述了在今后十年中地面天体测量的作用以及正在开展有关天球参考架的研究课题 ,同时也列出了我国正在和即将开展天体测量的几个研究课题  相似文献   

Report on Pontoniinae shrimps （Crustacea：Decapoda）collected by Joint Chinese—German Marine Biology Expeditions to Hainan lsland ,South China Sea：Ⅱ.Harpiliopsis,Ischnopontonia,Jocaste,Palae—mone     

李新正  刘瑞玉 《中国海洋湖沼学报》2003,21(2):154-165

The present paper reports 9 species of pontoniine shrimps collected from Hainan IS-land ,South China Sea ,by the Joint Chinese-German Marine Biology Expeditions,in which Peri-climenaeus arabicus (Calman,1939) and Periclimenaeus hecate (Nobili,1904) are recorded for the first time from Hainan Island.  相似文献   

地倾斜台站数字化、模拟观测数据频率特性研究   总被引：2，自引：2，他引：0  

李正媛  陈鹏  林穗平  陈志遥 《大地测量与地球动力学》2003,23(1):74-77

分析了四川、云南的部分地倾斜数字化观测与模拟观测资料的特性，从频率范围，周期10天以上的FFT谱特性，固体潮波振幅，相位特征值等方面，论述了地倾斜数字化资料较模拟记录资料的信息组成变化，并对资料的分析与应用进行了初步探讨。  相似文献   

CORROSION AND ADHERENCE AT SEA OIL-PLATFORM   总被引：1，自引：0，他引：1  

马士德  李言涛  黄修明  郑国安  常伟  吴培辉 《中国海洋湖沼学报》1995,13(3):253-257

Measurements of the adhesive corrosion and protective potentials of 3 Bohai Sea oilplatforms under different cathodic protection showed corrosion still occurred on these platforms undertheir designed protective potentials. Preliminary conclusions from indoor experiments on the relation betweenadhesive corrosion and cathodic protection showed adhering organisms caused the corrosion onthese platforms.  相似文献   

由两块拼接反射镜组成的光学系统的衍射能量分布     

徐文莉 《中国天文和天体物理学报》1997,(1)

LAMOST的光学系统由反射施密特改正板MA 和球面主镜MB 组成 ,MA 和MB 均为对角径 1 .1m的六角形子镜的拼镜面 .本文计算了星在子午圈上时望远镜观测不同的天区零视场和有视场时的光学系统衍射能量分布 ,还计算了MA 不变MB 改为整块的 6m镜以及MA 和MB 均为对角径 1 .5m的六角形子镜的拼镜面的衍射能量分布 .对这些计算结果进行分析 ,论证了六角形子镜尺寸选择的合理性  相似文献   

关于网络企业经济效益的评价理论研究     

岳正华  李黎 《成都信息工程学院学报》2001,16(3):192-198

网络经济是相对于大工业经济而言的，其网络企业经济效益的评价，可以从网络企业成本及收益两个方面讨论，然后以投入产出比等五个方面来建立其评价指标体系。  相似文献   

太阳表面米粒结构观测对比度分析     

饶长辉  姜文汉  凌宁 《天文学报》2001,42(2):134-139

分析以观测系统焦面上和探测器测到的太阳表面米粒结构的对比度与观测系统口径，大气湍流相干长度以及系统探测灵敏度的关系，给出了不同口径，不同大气湍流相干长度以及不同系统探测灵敏度时的米粒结构对比值计算结果，此外还给出了实验结果。  相似文献   

Petroleum Hydrocarbon Distribution Features in Water and Sediment off Fujian Shore   总被引：1，自引：0，他引：1  

王宪  徐鲁荣  李文权  李凌云  钱爱红 《中国海洋湖沼学报》2003,21(2):187-192

This study on the distribution features of petroleum hydrocarbon in water and sedi-ment off the Fujian shore using data obtained from the baseline research on oceanic contamination in Fujian showed that:during the research preiod,petroleum hydrocarbon concentrations in water varied from 5.77μg/L to 37.28μg/L,averaged 14.48 μg/L;was lower in the wet seasson than in the dry season;and was highest in the Minjiang Estuary and Jiulong Estuary in both seasons ,The petorleum hydrocarbon concentrations in shore sediment varied from 14.48 mg/kg to 784.36 mg/kg,averaged 133.3 mg/kg ,and was closely related to sediment types (granularity).  相似文献