分光光度法测定海洋河弧菌510脂多糖     

张运涛  顾谦群  方玉春  吕元萌  管华诗 《中国海洋大学学报(自然科学版)》2003,33(3):355-360

本文研究了直接测定海洋河弧菌 (vibrio fluvailis) 5 10脂多糖的分光光度法。利用 1,9-二甲基亚甲蓝 (DMB) ,在适当条件下同脂多糖发生染色反应 ,在 5 5 0 nm波长下 ,反应体系的吸光值同脂多糖含量呈线性相关。相关系数为 0 .9993,最低检出限为 10 μg/m L,相对标准偏差为 1.6 2 ,平均回收率为 (10 5± 2 .18) %。  相似文献   

均匀层浅海声场与海底反射损失的关系     

关定华 《海洋学报》1979,1(1):52-57

海底对浅海声传播的影响很大。海底有复杂的多层结构。在计算声场时如果把多层海底的影响都考虑进去,就会使计算复杂化,得不出一个清晰的物理图象。因此,在计算中往往使用海底反射损失系数来代替多层海底的模型。但海底反射损失与角度有复杂的依赖关系。为了计算方便,提出过不少简化模型。已经使用的模型有反射损失与角度无关、与角度一次方成正比;后来又提出反射损失在某一临界角之下与角度一次方成正比,在临界角之上与角度无关的三参数模型,克服了远场与近场衔接的问题[1,2]。但这些模型不一定在所有情况下都能反映海底反射损失与角度的关系的主要特征。本文提出一个近似方法,可以计算海底反射损失按任意规律随角度单调上升的情况下的声场。  相似文献   

Wave Decay and Its Characteristics in Surf Zone     

Gu Jialong Shen Xianrong Professor  Hohai University  Nanjing Engineer  The Investigation  Design Institute  Bureau of Communications of Zhejiang Province  Hangzhou 《中国海洋工程》1991,(2)

In accordance with the similarity between breaking waves and hydraulic jumps, the expressions for estimating wave decay and wave energy dissipation in the surf zone are derived based on the fundamental equations of fluid mechanics. Using the numerical solution of cnoidal wave theory, the various kinematic properties of waves in the surf zone, including the relative wave crest height, wave energy, and radiation stress are discussed. The values calculated with the method proposed in this paper are in good agreement with the experimental data gained by other researchers. The present expressions can be used in the studies of sediment transport on gently sloping beaches, especially on muddy beaches.  相似文献   

A New Pursuit Mode of Nonlinear Wave Surface     

孙大鹏李玉成  郭海滨 《中国海洋工程》2004,18(2):257-266

The numerical mode of nonlinear wave transformation based on both the Laplace equation for water field and the Bemoulli equation for water surface is a kind of time-domain boundary problem with initial conditions. And the basis for establishing the numerical mode of nonlinear wave in time domain is to trace the position of wave free surface and to calculale the instantaneous surface height and surface potential function. This paper firstly utilizes the ‘0-1‘ combined BEM to separate the boundary by means of discretization of Green‘ s integral equation based on the Laplace equation, then separates the free surface of wave with FEM and derives the FEM equation of wave surface that satisfies the nonlinear boundary conditions. By jointly solving the above BEM and FEM equations, the wave potential and surface height could be obtained with iteration in time domain. Thus a new kind of nonlinear numerical mode is established for calculating wave transformation. The wave test in the numerical wave tank shows that the numerical simulation with this mode is of high accuracy.  相似文献   

Characteristics of Scour and Deposition in Front of Breakwaters Under Irregular Broken Clapotis   总被引：1，自引：0，他引：1  

高学平张亚  李昌良 《中国海洋工程》2004,18(3):493-500

First the scour and deposition patterns of the sandy seabed in front of a vertical breakwater under the action of irregular broken clapotis are investigated experimentally and classified into five types: scour type Ⅰ , scour type Ⅱ, scour type Ⅲ, deposition type Ⅰ, and deposition type Ⅱ . Secondly, the processes of formation of scour and deposition patterns are probed in comparison with those induced by regular broken clapotis and standing waves. Thirdly, the criteria for distinguishing scour and deposition patterns under irregular broken clapotis are presented.  相似文献   

Modeling of Multi-Freedom Ship Motions in Irregular Waves with Fuzzy Neural Networks     

余建星  陆培毅  高喜峰  夏锦祝 《中国海洋工程》2003,17(2)

1 .IntroductionAshiptravelingatseaundergoesundesirablewave inducedmotions ,namely ,surge ,sway ,heav ing ,rolling ,pitchingandyaw .Thesemotionsoftencauseproblemstothecrew ,theonboardequip mentand ,intheworstcase ,thesafetyofthevessel.Tominimizethewave inducedshipmotions ,controlsystemsmaybeapplied .Theaccuratemodelingofshipmotionsisthereforeveryimportantforshipdesignanddesignofmotioncontrolsystems .Manyresearchershavedevelopedshipmotionpredictionmethodsbasedonthepotentialflowtheo ries (Dong ,…  相似文献   

A New Probability of Detection Model for Updating Crack Distribution of Offshore Structures     

李典庆  张圣坤  唐文勇 《中国海洋工程》2003,17(3)

1 .IntroductionNondestructiveinspection (NDI)isveryimportantforensuringthereliabilityofoffshorestructuresintheirservicelives (Lauraetal.,1 996 ) .Itiswellknownthatdetectionofflawsinvolvesconsider ablestatisticaluncertainties.Asaresult,theprobabilityofdetection (POD)forallflawsofagivensizehasbeenusedintheliteraturetodefinethecapabilityofaparticularNDItechniqueinagivenen vironment.SincethedataofPODusuallyscatterlargely ,itisdifficulttodeterminewhichmodelfitstheavailabledatabest.Thismodelun…  相似文献   

Action of Cnoidal Waves on Vertical Walls     

Qiu Dahong  Zang Jun  Jia Ying Academician of the Chinese Academy of Sciences  Professor  the State Key Laboratory of Coastal  Offshore Engineering 《中国海洋工程》1996,(2)

Based on the 2nd order cnoidal wave theory, the characters of shallow water standing waves and their action on vertical walls are studied in this paper. The theoretical expressions of the wave surface elevation in front of and the wave pressure on the vertical wall are obtained. In order to verify the theoretical results, model tests were made in the State Key Laboratory of Coastal and Offshore Engineering at DUT. For the wave surface elevation in front of the wall and the wave forces on the wall at the moment when the wave surface at the wall surface goes down to the bottom of the wave trough, the calculated results coincide quite well with the experimental results. For the wave forces on the wall at the moment when the wave surface at the wall surface goes up to the top of the wave crest, the theoretical expressions are modified by the experimental results. For the convenience of practical use, calculations are made for the wave conditions which usually occur in enginering practice by use of the inves  相似文献   

Relationship between biogeochemical features of biogenic elements and flocculation in the Changjiang Estuary   总被引：1，自引：3，他引：1  

Lin Yi''an  Tang Renyou  Li Yan  Dong Henglin  Guan Xuwei  Chen Yinzu 《海洋学报(英文版)》1995,14(2):225-234

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海湾扇贝肝素样多糖分离方法的研究     

王长云  管华诗  林洪 《海洋与湖沼》1995,26(S1):66-69

于1992年1月-1993年2月，对从海湾扇贝中提取获得的糖胺聚糖（GAG）用乙醇和溴化十六烷基三甲铵（CTAB）分级分离，并对所得制品用琼脂糖凝胶电泳和高效液相色谱验证，以研究一种简便、快速的分离提纯方法。结果表明，扇贝GAG水溶液在乙醇量60％时分离获得的级分，其GAG－GTAB给合物再用2.0 mol/LNaCl溶液解离得到的主要级分，琼脂糖凝胶电泳和高效液相色谱均显示出单一区带，红外光谱类似干肝素的。证明这一提纯过程是一简便、快速的分离提纯方法。  相似文献