Cloning,characterization,and expression analysis of the DEAD-box family genes,Fc-vasa and Fc-PL10a,in Chinese shrimp (Fenneropenaeus chinensis)     

周倩如  邵明瑜  秦贞奎  康庆浩  张志峰 《中国海洋湖沼学报》2010,(1)

RNA helicases of the DEAD-box and related families are involved in various cellular processes including DNA replication,DNA repair,and RNA processing.However,the function of DEAD-box proteins in aquaculture species is poorly understood at molecular level.We obtained the full-length cDNA sequences of two genes encoding helicase-related proteins,Fc-vasa and Fc-PL10a,from the testes of Chinese shrimp,Fenneropenaeus chinensis.The two predicted amino acid sequences contain all the conserved motifs characterized ...  相似文献   

Distribution and expression in vitro and in vivo of DNA vaccine against lymphocystis disease virus in Japanese flounder(Paralichthys olivaceus)   总被引：1，自引：0，他引：1  

郑风荣  孙修勤  刘洪展  吴兴安  钟楠  王波  周国栋 《中国海洋湖沼学报》2010,(1)

Lymphocystis disease,caused by the lymphocystis disease virus (LCDV),is a significant worldwide problem in fish industry causing substantial economic losses.In this study,we aimed to develop the DNA vaccine against LCDV,using DNA vaccination technology.We evaluated plasmid pEGFP-N2-LCDV1.3 kb as a DNA vaccine candidate.The plasmid DNA was transiently expressed after liposome transfection into the eukaryotic COS 7 cell line.The distribution and expression of the DNA vaccine (pEGFP-N2-LCDV1.3kb) were also ana...  相似文献   

Impacts of the Nakhodka heavy-oil spill on an intertidal ecosystem: an approach to impact evaluation using geographical information system     

Teruhisa K  Masahiro N  Hiroshi K  Tomoko Y  Kouichi O;Marine Life Research Group of Takeno 《Marine pollution bulletin》2003,47(1-6):99-104

A major heavy-oil spill from the Russian tanker Nakhodka occurred in the Sea of Japan on 2 January 1997. We investigated the impacts of this spill on a rocky intertidal ecosystem along the southern coast of the Sea of Japan. We selected Imago-Ura Cove as our study site to observe temporal changes along the oiled shore, because minimal cleaning effort was made in this area. Field surveys were conducted every autumn and spring from 1997 to 2000. We measured coverage by macroalgae in 1 x 1-m(2) quadrats and counted the animals in 5 x 5-m(2) quadrats along the intertidal zone. Changes in the ecosystem caused by the oil spill were analyzed by applying a geographical information system (GIS) to the Sea of Japan for the first time. The GIS showed that following the accident there were heavily oiled areas in sheltered regions, but these decreased over the three years. It also showed that coverage by macroalgae and the number of animals increased, although some species of algae with microscopic sporophyte generations, and some populations of perennial shellfish, remained stable or decreased during the study period. GIS was able to trace temporal changes in intertidal communities resulting from the impacts of heavy oil on flora and fauna at a spatial scale of 10-100 m. GIS is thus a practical tool for visualizing, analyzing, and monitoring changes in an ecosystem polluted by oil, taking into account topographic differences along the coastline.  相似文献   

New British unmanned submersible will slash underwater inspection costs     

Marine Unit Technology Ltd. 《Ocean Engineering》1979,6(3):341-343

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TTI介质相速度理论研究     

ZHAO Feng-fan HAN Li-guo LIN Yan XUAN Yi-hua .College of GeoExploration of Science  Technology  Jilin University  Changchun  China .Third Marine Geology Survey Party  Shanghai Offshore Petroleum Bureau  SINOPEC  Shanghai  China 《吉林大学学报(地球科学版)》2006,(Z2)

依据三维TTI介质的相速度表达式,模拟并分析了三维TTI介质相速度图像。并且考查了不同的各向异性系数对TTI介质相速度的影响,为三维的TTI介质相速度的分析提供了理论依据,具有一定的实际应用价值。  相似文献   

The Paleoenvironment and Lithic Taphonomy of Shi’Bat Dihya 1, a Middle Paleolithic Site in Wadi Surdud,Yemen     

Luca Sitzia  Pascal Bertran  Stphane Boulogne  Michel Brenet  Rmy Crassard  Anne Delagnes  Marine Frouin  Christine Hatt  Jacques Jaubert  Lamya Khalidi  Erwan Messager  Norbert Mercier  Alain Meunier  Stphane Peign  Alain Queffelec  Chantal Tribolo  Roberto Macchiarelli 《Geoarchaeology》2012,27(6):471-491

The Shi'bat Dihya 1 site in western Yemen, dated by optically stimulated luminescence to 55 ka, provides insight into the Middle Paleolithic peopling of the Arabian Peninsula. The archaeological layer is interstratified within thick, sandy silt floodplain deposits filling a piedmont basin. Luminescence dates, lack of soil development, and gypsum precipitation indicate a high accretion rate of the floodplain during Marine Isotope Stage 3, in connection with a (semi)‐arid environment. Rapid overbank sedimentation was likely a result of the remobilization of loess material deposited on the Yemeni Great Escarpment at the periphery of the adjacent Tihama coastal sand desert or of other sources. Fabric and size analyses of the lithic artifacts, together with spatial projections, indicate site modifications by floods. Primary modifications include (1) selective accumulation of medium‐sized lithic pieces as a result of hydraulic sorting, (2) bimodal orientation of artifacts, and (3) ripple‐like arrangement of lithics and bone/tooth fragments. The overrepresentation of teeth may also be a consequence of sorting. Although floods have distorted the original site patterning, long‐distance transport of artifacts by water can be excluded, as indicated by relatively high refitting rate, close proximity of artifacts derived from the same block of raw material, and lack of abrasion of the pieces. Therefore, the site is considered “geologically” in situ because its remobilization by water occurred shortly after human abandonment. This study also stresses that the effective preservation of a site cannot be assessed without careful taphonomic study, even in a potentially favorable depositional context such as silty alluvium.  相似文献   

Screen and effect analysis of immunostimulants for sea cucumber,Apostichopus japonicus     

李继业  孙修勤  郑风荣  郝林华 《中国海洋湖沼学报》2009,27(1)

Immunostimulants may improve disease resistance of aquaculture animals by promoting the nonspecific immunity response of the organisms. Five types of saccharides, including chitosan, yeast polysaccharide, burdock oligosaccharide, seaweed polysaccharide and lentinus edodes polysaccharide, were screened for potential use as immunostimulants by using spectrophotometry. The saccharides were injected into Apostichopus japonicus, a sea cucumber, and the lysozyme and superoxide dismutase (SOD) activities of the coelomic fluid and epidermal slime were monitored in six consecutive days. The results show that the lysozyme activity of the animal's coelomic fluid was significantly stimulated on day 2, day 4 and day 6 after the injection of the saccharides (P<0.05). The effects of chitosan and yeast polysaccharide were the most notable. The lysozyme activity of the epidermal slime was significantly increased by chitosana, yeast polysaccharide, seaweed polysaccharide, and burdock oligosaccharide on day 1 and day 2 after the injection (P<0.05). The SOD activity of the coelomic fluid was significantly promoted by the saccharides on day 2 and day 4 post-injection (P<0.05), while the SOD activity of the epidermal slime increased on day 2. These findings indicate that chitosan and yeast polysaccharide are the most effective immunostimulants and potential healthy anti-disease feedstuff for A. japonicus.  相似文献