Isolation,biodegradation ability and molecular detection of hydrocarbon degrading bacteria in petroleum samples from a Brazilian offshore basin     

Suzan Pantaroto de Vasconcellos  Elaine Crespim  Georgiana Feitosa da Cruz  Diego Barbosa Senatore  Karen Christina Marques Simioni  Eugênio Vaz dos Santos Neto  Anita Jocelyne Marsaioli  Valéria Maia de Oliveira 《Organic Geochemistry》2009,40(5):574-588

The detection of microorganisms with potential for biodeterioration and biodegradation in petroleum fields is of great relevance, since these organisms may be related to a decrease in petroleum quality in the reservoirs or damage in the production facilities. In this sense, petroleum formation water and oil samples were collected from the Campos Basin, Brazil, with the aim of isolating microorganisms and evaluating their ability to degrade distinct classes of hydrocarbon biomarkers (9,10-dihydrophenanthrene, phytane, nonadecanoic acid and 5α-cholestane). Twenty eight bacterial isolates were recovered and identified by sequencing their 16S rRNA genes. Biodegradation assays revealed that bacterial metabolism of hydrocarbons occurred through reactions based on oxidation, carbon–carbon bond cleavage and generation of new bonds or by the physical incorporation of hydrocarbons into microbial cell walls. Based on the biodegradation results, selective PCR-based systems were developed for direct detection in petroleum samples of bacterial groups of interest, namely Bacillus spp., Micrococcus spp., Achromobacter xylosoxidans, Dietzia spp. and Bacillus pumilus. Primer sets targeting 16S rRNA genes were designed and their specificity was confirmed in silico (i.e. computational analysis) and in PCR reactions using DNA from reference strains as positive and negative controls. Total DNA from oil was purified and the amplification tests revealed the presence of the target bacteria in the samples, unraveling a significant potential for petroleum deterioration in the reservoirs sampled, once proper conditions are present for hydrocarbon degradation. The application of molecular methods for rapid detection of specific microorganisms in environmental samples would be valuable as a supporting tool for the evaluation of oil quality in production reservoirs.  相似文献   

A new analysis of the GJ581 extrasolar planetary system     

M. Tadeu dos Santos  G. G. Silva  S. Ferraz-Mello  T. A. Michtchenko 《Celestial Mechanics and Dynamical Astronomy》2012,113(1):49-62

We have done a new analysis of the available observations of the GJ581 exoplanetary system. Today this system is controversial due to choices that can be done in the orbital determination. The main ones are the occurrence of aliases and the additional bodies??the planets f and g??announced in Vogt et?al. (Astrophys J 723:954?C965, 2010). Any dynamical study of exoplanets requires the good knowledge of the orbital elements and the investigations involving the planet g are particularly interesting, since this body would lie in the habitable zone (HZ) of the star GJ581. This region, for this system, is very attractive of the dynamical point of view due to several resonances of two and three bodies present there. In this work, we investigate the conditions under which the planet g may exist. We stress the fact that the planet g is intimately related with the orbital elements of the planet d; more precisely, we conclude that it is not possible to disconnect its existence from the determination of the eccentricity of the planet d. Concerning the planet f, we have found one solution with period ??450?days, but we are judicious about any affirmation concerning this body because its signal is in the threshold of detection and the high period is in a spectral region where the occurrence of aliases is very common. Besides, we outline some dynamical features of the HZ with the dynamical map and point out the role played by some resonances laying there.  相似文献   

Diversity and validity of stable-unstable transitions in the algorithmic weak stability boundary     

Priscilla A. Sousa Silva  Maisa O. Terra 《Celestial Mechanics and Dynamical Astronomy》2012,113(4):453-478

This paper is devoted to verify the consistency of the algorithmic Weak Stability Boundary definition concerning the achievement of capture-escape detection, through examining the transitions produced by the implementation of this definition. Our main goal is to show that many types of spurious transitions concerning capture-escape behavior are found besides the expected transitions due to the separatrix role of the hyperbolic invariant manifolds of the central manifold of the collinear equilibria of the Planar Circular Restricted Three-Body Problem. We identify and characterize authentic and spurious transitions and discuss their spatial distribution along the boundary for sets of initial conditions with high eccentricity, showing the frequent occurrence of spurious transitions and of collisional trajectories. Also, we investigate smooth and fractal-like portions of the boundary. Finally, we propose an alternative stability boundary definition based on the effective detection of capture-escape transitions.  相似文献   

Water Availability of Sub-Basins with Restrictions on Use under Climate Change Scenarios in Brazil     

Redivo  Polyana Comino  Sanches  Luciana  de Carvalho Alves  Marcelo  Barbosa da Silva  Jhonatan 《Water Resources》2021,48(6):905-913

Water Resources - The Queima Pé Stream Basin (QPSB) and Ararão River Basin (ARB) in central Brazil have had recurrent problems with water scarcity. This may get worse under anthropogenic...  相似文献   

Camacho inlet southern Brazil - Instability causes and user conflicts     

Guilherme Vieira da Silva João Luiz Baptista de Carvalho  Antonio Henrique da Fontoura Klein 《Ocean & Coastal Management》2011,54(8):569-576

Inlet stabilization is a common practice in Brazil, as it is worldwide. In this paper we present results of the analysis of the stabilization practices of the Camacho Inlet, located in Santa Catarina State, southern Brazil. This inlet is maintained intermittently, in accordance with a regime designed to benefit both local fishermen and rice farmers. Fishermen require this inlet to be open as a navigation channel during the fishing season; rice farmers needs it closed during the irrigation period to allow the pumping of fresh water from the Congonhas River which drains into the Camacho’s backbarrier lagoon. An additional complication arises from the tendency of this inlet to close naturally due to infilling by dune migration. Aerial images spanning a 65 year time period (1938-2003) were analyzed, demonstrating the variability in the inlet’s width between 0 (closed) and 360 m. The analysis of inlet stability and user needs indicate that an appropriate solution is the implementation of an annual inlet dredging program in the austral autumn. This would allow for the inlet to remain open from May to September and then close naturally for the rest of the year, providing for a backbarrier fresh water reservoir during the irrigation period.  相似文献   

A simple model to interpret the ultraviolet, optical and infrared emission from galaxies     

Elisabete da Cunha  Stéphane Charlot  David Elbaz 《Monthly notices of the Royal Astronomical Society》2008,388(4):1595-1617

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Binary collisions and the slingshot effect     

Amaro J. Rica da Silva  José P. S. Lemos 《Celestial Mechanics and Dynamical Astronomy》2008,100(3):191-208

We derive the equations for the gravity assist manoeuvre in the general 2D case without the constraints of circular planetary orbits or widely different masses as assumed by Broucke (AIAA/AAS 1988) and obtain the slingshot conditions and maximum energy gain for arbitrary mass ratios of two colliding rigid bodies. Using the geometric view developed in an earlier paper by the authors (Rica da Silva, A., Lemos, J.P.S.: Am. J. Phys. 74, 584–590, 2006) the possible trajectories are computed for both attractive or repulsive interactions yielding a further insight on the slingshot mechanics and its parametrization. . The general slingshot manoeuvre for arbitrary masses is explained as a particular case of the possible outcomes of attractive or repulsive binary collisions, and the correlation between asymptotic information and orbital parameters is obtained in general.  相似文献   

Stratigraphic evolution of the nearshore to fluvial plain of the Upper Cuyo Group,Neuquén,Argentina     

Henrique P. Kern  Ernesto L. C. Lavina  Paulo S. G. Paim  Hctor A. Leanza 《Sedimentology》2019,66(7):2686-2720

The upper portion of the Cuyo Group in the Zapala region, south‐eastern Neuquén Basin (Western Argentina), encompasses marine and transitional deposits (Lajas Formation) overlain by alluvial rocks (Challacó Formation). The Challacó Formation is covered by the Mendoza Group above a second‐order sequence boundary. The present study presents the stratigraphic framework and palaeophysiographic evolution of this Bajocian to Eo‐Calovian interval. The studied succession comprises the following genetic facies associations: (i) offshore and lower shoreface–offshore transition; (ii) lower shoreface; (iii) upper shoreface; iv) intertidal–subtidal; (v) supratidal–intertidal; (vi) braided fluvial to delta plain; (vii) meandering river; and (viii) braided river. The stratigraphic framework embraces four third‐order depositional sequences (C1 to C4) whose boundaries are characterized by the abrupt superposition of proximal over distal facies associations. Sequences C1 to C3 comprise mostly littoral deposits and display well‐defined, small‐scale transgressive–regressive cycles associated with fourth‐order depositional sequences. Such high‐frequency cycles are usually bounded by ravinement surfaces associated with transgressive lags. At last, the depositional sequence C4 delineates an important tectonic reorganization probably associated with an uplift of the Huincul Ridge. This is suggested by an inversion of the transport trend, north‐westward during the deposition of C1 to C3 depositional sequences (Lajas Formation) to a south‐west trend during the deposition of the braided fluvial strata related to the C4 depositional sequence (Challacó Formation).  相似文献   

Metals in eggs and hatchlings of Dermochelys coriacea (testudines) in Espírito Santo,Brazil and their relation to hatching and emergence success     

Jordana Borini Freire  Rafael M. Sousa  Karla Taufner  Maria TW Carneiro  Henrique R Filgueiras  Dominik Lenz  Paulo Dias Ferreira  Denise C Endringer 《Marine Ecology》2021,42(1):e12633

Certain concentration of metal may influence survival in embryonic stages, reducing the birthrate and hatching emergence. This study aimed to evaluate concentration of metal in eggs and hatchlings of Dermochelys coriacea and correlate metal concentrations in eggs with hatching and emergence success. The samples were collected at Biological Reserve Station, ES, Brazil. At the nests' opening, unhatched eggs and stillborn hatchlings were collected to survey the successful hatching and emergence of D. coriacea. The eggs shell, egg content, and dead hatchlings metal concentrations were compared to hatching and emergence success. A positive correlation was found between the concentration of copper (Cu) in the hatchlings and the success of the emergence (r² = .28, p < .05), and a moderate positive correlation between the concentrations of iron (Fe) and barium (Ba; r² = .44, p < .05) and success hatching. The concentrations of metals in eggs and hatchlings of D. coriacea in the Espírito Santo are below the levels that could be considered harmful to the species. In summary, there is a positive correlation between metals levels and hatching and emergence success.  相似文献   

Late Quaternary vegetation and climate dynamics in central-eastern Brazil: insights from a ~35k cal a bp peat record in the Cerrado biome     

Ingrid Horák-Terra  Antonio Martínez Cortizas  Cynthia Fernandes Pinto Da Luz  Alexandre Christófaro Silva  Tim Mighall  Plínio Barbosa De Camargo  Carlos Victor Mendonça-Filho  Paulo Eduardo De Oliveira  Francisco Willian Cruz  Pablo Vidal-Torrado 《第四纪科学杂志》2020,35(5):664-676

The late Quaternary evolution of central-eastern Brazil has been under-researched. Questions remain as to the origin of the Cerrado, a highly endangered biome, and other types of vegetation, such as the Capões – small vegetation islands of semi-deciduous and mountain forests. We investigated the factors that influenced the expansion and contraction of the Cerrado and Capões during the late Quaternary (last ~35 ka), using a multi-proxy approach: stable isotopes (δ¹³C, δ¹⁵N), geochemistry, pollen and multivariate statistics derived from a peat core (Pinheiro mire, Serra do Espinhaço Meridional). Five major shifts in precipitation, temperature, vegetation and landscape stability occurred at different timescales. Our study revealed that changes in the South Atlantic Convergence Zone (SACZ) seem to have been coeval with these shifts: from the Late Glacial Maximum to mid-Holocene the SACZ remained near (~29.6 to ~16.5k cal a bp ) and over (~16.5 to ~6.1 k cal a bp ) the study area, providing humidity to the region. This challenges previous research which suggested that climate was drier for this time period. At present, the Capões are likely to be a remnant of a more humid climate; meanwhile, the Cerrado biome seems to have stablished in the late Holocene, after ~3.1 k cal a bp .  相似文献