Spatial profiles of hsp70 proteins in Asian clam (Potamocorbula amurensis) in northern San Francisco Bay may be linked to natural rather than anthropogenic stressors.   总被引：1，自引：0，他引：1  

I Werner  D E Hinton 《Marine environmental research》2000,50(1-5):379-384

Multi-year investigations in northern San Francisco Bay by United States Geological Survey have linked reduced condition indices in populations of Asian clam (Potamocorbula amurensis) with elevated cadmium tissue concentrations. Our study seeks to determine whether levels of hsp70 proteins in P. amurensis can be correlated with these findings, and/or are related to histopathologic alterations and concentrations of metallothionein-like proteins. Here we present our results on stress proteins in clams collected monthly from four field stations between July 1996 and January 1998. In addition, animals were exposed in the laboratory to a range of salinities. Stress proteins were analyzed by Western blotting using monoclonal antibodies. Hsp70 protein levels in field-collected clams were significantly higher at the seaward (high salinity/low cadmium) stations (12.5, 8.1) than at the landward (low salinity/high cadmium) stations (6.1, 4.1). Laboratory studies showed that clams exposed to 0.1 ppt salinity had markedly lower hsp70 levels than clams exposed to higher salinities. In view of our previous laboratory studies showing that cadmium induces hsp70 in P. amurensis, our present results indicate that reduced hsp70 protein levels in field-collected clams may be linked to salinity effects rather than cadmium tissue concentrations.  相似文献   

The discovery of photospheric nickel in the hot DO white dwarf REJ 0503−289     

M. A. Barstow  S. Dreizler  J. B. Holberg  D. S. Finley  K. Werner  I. Hubeny  E. M. Sion 《Monthly notices of the Royal Astronomical Society》2000,314(1):109-122

We present the first evidence for the direct detection of nickel in the photosphere of the hot DO white dwarf REJ 0503−289. While this element has been seen previously in the atmospheres of hot H-rich white dwarfs, this is one of the first similar discoveries in a He-rich object. Intriguingly, iron, which is observed to be more abundant than Ni in the hot DA stars, is not detected, the upper limit to its abundance (Fe/He=10⁻⁶) implying an Fe/Ni ratio a factor of 10 lower than seen in the H-rich objects (Ni/He=10⁻⁵ for REJ 0503−289). The abundances of nickel and various other elements heavier than He were determined from Goddard High Resolution Spectrograph spectra. We used two completely independent sets of non-local thermodynamic equilibrium model atmospheres, which both provide the same results. This not only reduces the possibility of systematic errors in our analysis, but is also an important consistency check for both model atmosphere codes.
We have also developed a more objective method of determining T _eff and log  g , from the He lines in the optical spectrum, in the form of a formal fitting of the line profiles to a grid of model spectra, an analogue of the standard procedure utilizing the Balmer lines in DA white dwarfs. This gives the assigned uncertainties in T _eff and log  g a firm statistical basis and allows us to demonstrate that inclusion of elements heavier than H, He and C in the spectral calculations, exclusively considered in most published optical analyses, yields a systematic downward shift in the measured value of T _eff.  相似文献   

Moisture origin and stable isotope characteristics of precipitation in southeast Siberia     

Svetlana S. Kostrova  Hanno Meyer  Francisco Fernandoy  Martin Werner  Pavel E. Tarasov 《水文研究》2020,34(1):51-67

The paper presents oxygen and hydrogen isotopes of 284 precipitation event samples systematically collected in Irkutsk, in the Baikal region (southeast Siberia), between June 2011 and April 2017. This is the first high-resolution dataset of stable isotopes of precipitation from this poorly studied region of continental Asia, which has a high potential for isotope-based palaeoclimate research. The dataset revealed distinct seasonal variations: relatively high δ¹⁸O (up to −4‰) and δD (up to −40‰) values characterize summer air masses, and lighter isotope composition (−41‰ for δ¹⁸O and −322‰ for δD) is characteristic of winter precipitation. Our results show that air temperature mainly affects the isotope composition of precipitation, and no significant correlations were obtained for precipitation amount and relative humidity. A new temperature dependence was established for weighted mean monthly precipitation: +0.50‰/°C (r² = 0.83; p <.01; n = 55) for δ¹⁸O and +3.8‰/°C (r² = 0.83, p < 0.01; n = 55) for δD. Secondary fractionation processes (e.g., contribution of recycled moisture) were identified mainly in summer from low d excess. Backward trajectories assessed with the Hybrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT) model indicate that precipitation with the lowest mean δ¹⁸O and δD values reaches Irkutsk in winter related to moisture transport from the Arctic. Precipitation originating from the west/southwest with the heaviest mean isotope composition reaches Irkutsk in summer, thus representing moisture transport across Eurasia. Generally, moisture transport from the west, that is, the Atlantic Ocean predominates throughout the year. A comparison of our new isotope dataset with simulation results using the European Centre/Hamburg version 5 (ECHAM5)-wiso climate model reveals a good agreement of variations in δ¹⁸O (r² = 0.87; p <.01; n = 55) and air temperature (r² = 0.99; p <.01; n = 71). However, the ECHAM5-wiso model fails to capture observed variations in d excess (r² = 0.14; p < 0.01; n = 55). This disagreement can be partly explained by a model deficit of capturing regional hydrological processes associated with secondary moisture supply in summer.  相似文献   

Transient Recharge Estimability Through Field‐Scale Groundwater Model Calibration          下载免费PDF全文

Matthew J. Knowling  Adrian D. Werner 《Ground water》2017,55(6):827-840

The estimation of recharge through groundwater model calibration is hampered by the nonuniqueness of recharge and aquifer parameter values. It has been shown recently that the estimability of spatially distributed recharge through calibration of steady‐state models for practical situations (i.e., real‐world, field‐scale aquifer settings) is limited by the need for excessive amounts of hydraulic‐parameter and groundwater‐level data. However, the extent to which temporal recharge variability can be informed through transient model calibration, which involves larger water‐level datasets, but requires the additional consideration of storage parameters, is presently unknown for practical situations. In this study, time‐varying recharge estimates, inferred through calibration of a field‐scale highly parameterized groundwater model, are systematically investigated subject to changes in (1) the degree to which hydraulic parameters including hydraulic conductivity (K) and specific yield (S_y) are constrained, (2) the number of water‐level calibration targets, and (3) the temporal resolution (up to monthly time steps) at which recharge is estimated. The analysis involves the use of a synthetic reality (a reference model) based on a groundwater model of Uley South Basin, South Australia. Identifiability statistics are used to evaluate the ability of recharge and hydraulic parameters to be estimated uniquely. Results show that reasonable estimates of monthly recharge (<30% recharge root‐mean‐squared error) require a considerable amount of transient water‐level data, and that the spatial distribution of K is known. Joint estimation of recharge, S_y and K, however, precludes reasonable inference of recharge and hydraulic parameter values. We conclude that the estimation of temporal recharge variability through calibration may be impractical for real‐world settings.  相似文献   

Zielvorgaben für Pflanzenschutzmittelwirkstoffe und andere Schadstoffe in Oberflächengewässern     

Jutta Jahnel  Christian Zwiener  Thomas J. Gremm  Gudrun Abbt‐Braun  Fritz H. Frimmel  Carola Kussatz  Dieter Schudoma  Werner Rocker 《洁净——土壤、空气、水》2001,29(4):246-253

Quality Targets for Pesticides and Other Pollutants in Surface Waters Surface waters are not only adversely affected by industrial and municipal wastewaters but also by diffuse sources. For the control of the ecological water quality, so‐called quality targets are set. The quality targets are threshold concentrations for hazardous substances related to the protected goods like water ecosystems, drinking water supply, sediments, or fishing. In this paper, quality targets for the protection of aquatic life in surface waters were derived on the basis of a concept developed by the International Commission for the Protection of the Rhine (Internationale Kommission zum Schutze des Rheins – IKSR). The quality targets for the selected hazardous substances, which mainly belong to the chemical class of pesticides, were calculated using ecotoxicological results for species of bacteria, algae, fish, and small crustaceans, as the four trophic standards of the water ecosystem. In cooperation with the Federal Environmental Agency (Umweltbundesamt – UBA) the effect data were taken from ecotoxicological data bases. According to the concept of the IKSR, the lowest test result for the most sensitive species was multiplied by an assessment factor. This proceeding concerns the difficulty in describing the complex interactions in water ecosystems with toxicity data of single laboratory experiments for a few organisms. For seven pesticides quality targets below 0.1 μg/L were proposed. These results show that the ecotoxicologically based quality targets might be even lower than the limit values for pesticides in drinking water of 0.1 μg/L. But for most of the substances the determined values are significantly higher. The great concentration range of quality targets demonstrates distinctly that one standard concentration for all pesticides could not be given with regard to the different effects on aquatic organisms.  相似文献