Microalgae, a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical, bioenergy and food applications. This study aims to investigate the sugaring-out effect on the recovery of protein from wet green microalga, Chlorella sorokiniana CY1 which was assisted with sonication. A comparison of monosaccharides and disaccharides as one of the phaseforming constituents shows that the monosaccharides, glucose was the most suitable sugar in forming the phases with acetonitrile to enhance the production of protein(52% of protein). The protein productivity of microalgae was found to be significantly influenced by the volume ratio of both phases, as the yield of protein increased to 77%. The interval time between the sonication as well as the sonication modes were influencing the protein productivity as well. The optimum protein productivity was obtained with 10 s of resting time in between sonication. Pulse mode of sonication was suitable to break down the cell wall of microalgae compared to continuous mode as a lower protein yield was obtained with the application of continuous mode. The optimum condition for protein extraction were found as followed: 200 g/L glucose as bottom phase with volume ratio of I:1.25, 10 s of resting time for ultrasonication, 5 s of ultrasonication in pulse mode and 0.25 g of biomass weight. The high yield of protein about 81% could be obtained from microalgae which demonstrates the potential of this source and expected to play an important role in the future. 相似文献
Based on in-situ observation, satellite and reanalysis data, responses of the western North Pacific subtropical ocean(WNPSO) to the slow-moving category 5 super typhoon Nanmadol in 2011 are analyzed. The dynamical response is dominated by near-inertial currents and Ekman currents with maximum amplitude of 0.39 m/s and 0.15 m/s, respectively. The near-inertial currents concentrated around 100 m below the sea surface and had an e-folding timescale of 4 days. The near-inertial energy propagated both upward and downward, and the vertical phase speed and wavelength were estimated to be 5 m/h and 175 m, respectively. The frequency of the near-inertial currents was blue-shifted near the surface and redshifted in ocean interior which may relate to wave propagation and/or background vorticity. The resultant surface cooling reaches -4.35℃ and happens when translation speed of Nanmadol is smaller than 3.0 m/s.When Nanmadol reaches super typhoon intensity, the cooling is less than 3.0℃ suggesting that the typhoon translation speed plays important roles as well as typhoon intensity in surface cooling. Upwelling induced by the slow-moving typhoon wind leads to typhoon track confined cooling area and the right-hand bias of cooling is slight. The mixed layer cooling and thermocline warming are induced by wind-generated upwelling and vertical entrainment. Vertical entrainment also led to mixed layer salinity increase and thermocline salinity decrease, however, mixed layer salinity decrease occurs at certain stations as well. Our results suggest that typhoon translation speed is a vital factor responsible for the oceanic thermohaline and dynamical responses, and the small Mach number(slow typhoon translation speed) facilitate development of Ekman current and upwelling. 相似文献
Analysis of 2D seismic data over 4 500 km in length from the Madura Strait Basin in the East Java Sea reveals seismic reflection characteristics of reefs and associated sedimentary bodies, including asymmetrical or symmetrical dome reflections, slope progradational reflections, chaotic reflections and discontinuous strong reflections inside the reef, which onlap the flank of the reef. It is concluded that the developmental paleo-environment of most reefs is mainly conducive to shallow marine carbonate platform facies and platform margin facies, based on well core data, variations in seismic facies and strata thickness. The formation and evolution of all reefs are primarily influenced by the tectonic framework of the Madura Strait Basin. Platform margin reefs are principally controlled by two types of structures: one is a series of E-W trending Paleogene normal faults, and the other is an E-W trending Neogene inversion structures. In addition, wave actions, tidal currents and other ocean currents play an accelerated role in sorting, rounding and redeposition for the accumulation and evolution of reefs. Tertiary reefs in the MSB can be divided into four types: 1) an open platform coral reef of Late Oligocene to Early Miocene, 2) a platform margin coral reef controlled by normal faults in Late Oligocene to Early Miocene, 3) a platform margin Globigerina moundreef controlled by a “hidden” inversion structure in Early Pliocene, and 4) a platform margin Globigerina mound-reef controlled by thrust faults in the early Pliocene. Patterns of the formation and evolution of reefs are also suggested.
Journal of Oceanology and Limnology - Blepharipoda liberate Shen is a commercially valuable seafood species that has important ecological significance in Shandong Province, China. Although B.... 相似文献
Journal of Geographical Sciences - Rainfall interception is of great significance to the fully utilization of rainfall in water limited areas. Until now, studies on rainfall partitioning process of... 相似文献
Journal of Geographical Sciences - The Interconnected River System Network (IRSN) plays a crucial role in water resource allocation, water ecological restoration and water quality improvement. It... 相似文献
Existing spatial clustering methods primarily focus on points distributed in planar space. However, occurrence locations and background processes of most human mobility events within cities are constrained by the road network space. Here we describe a density-based clustering approach for objectively detecting clusters in network-constrained point events. First, the network-constrained Delaunay triangulation is constructed to facilitate the measurement of network distances between points. Then, a combination of network kernel density estimation and potential entropy is executed to determine the optimal neighbourhood size. Furthermore, all network-constrained events are tested under a null hypothesis to statistically identify core points with significantly high densities. Finally, spatial clusters can be formed by expanding from the identified core points. Experimental comparisons performed on the origin and destination points of taxis in Beijing demonstrate that the proposed method can ascertain network-constrained clusters precisely and significantly. The resulting time-dependent patterns of clusters will be informative for taxi route selections in the future. 相似文献