固定化鲅鱼乙酰胆碱酯酶的制备及部分性质测定   总被引：3，自引：0，他引：3  

孟范平  何东海  朱小山  马冬冬  杨正先  YANG Zheng-Xian 《中国海洋大学学报(自然科学版)》2005,35(6):1067-1071

酶的固定化是酶传感器制备过程中重要的1个环节.研究采用直接共价法固定鲅鱼乙酰胆碱酯酶（AChE）.制备方法为：0.1g CNBr活化的Sepharose 4B凝胶用1mmol/L的HCl充分溶胀后,与活力为10U的AChE溶液混合,于4℃下150r/min振荡8h.所制备的固定化酶活力回收率较高（96%）,对pH值和温度变化的适应能力均优于非固定化酶;在3个月保存期内,前者的活力损失13%,而后者的活力则下降89%.这说明固定过程能够大大提高AChE的抗逆性和保持酶活力的稳定,有利于酶传感器的制备.  相似文献   

南麂站定常波风浪波高与风速的经验关系     

黄树生 《海洋学研究》1994,12(4):1-8

本文以南麂海洋站１９８３～１９９０年风、浪的实测资料为依据，建立了南麂海城春、夏、秋、冬季定常波风浪波高与风速的经验关系式。检验结果表明，曲线回归显著，计算值与实测值吻合良好。文中还对偏ＮＮＥ向和偏ＳＳＷ向计算波高随风速增大的快慢，同一方向在同一风速作用下计算波高的季节变化及其机理作了初浅的讨论。  相似文献   

云贵高原湖泊沉积物─水界面碱度扩散通量研究     

吴丰昌  万国江  黄荣贵 《海洋与湖沼》1997,(1)

于1991-1995年间5次在云贵高原泸沽湖、洱海湖和贵州阿哈湖、百花湖的湖心采集沉积物柱芯、界面水和湖水样品，通过其pH值和HCO-3浓度剖面及界面碱度扩散通量的研究，首次定量评估高原湖泊界面扩散作用上覆水体碱度的影响程度。研究结果表明，云贵高原某些湖水寄宿时间相对较长、湖水深度相对小的湖泊，界面扩散作用是水体碱度的重要来源之一;湖水寄宿时间较短、深度较小的湖泊，界面扩散对上覆水体的影响可以忽略不计。  相似文献   

大鹏湾夜光藻种群的季节变化和分布特征     

黄长江  齐雨藻  杞桑  吕颂辉  江天久 《海洋与湖沼》1996,27(5):493-498

于１９９０年３月－１９９２年６月对中国南海大鹏湾盐田附近海域的夜光藻种群动态及时空分布进行了综合调查，结果表明，夜光藻种群的出现期间一般为每年的１－６月，最早是１９９１年的１２月，娄量高峰期为３－５月，种群密度的波动极为显著；调查期间共发生８次赤潮，且都发生在３月初至５月初；大鹏湾夜光藻赤潮的发生特点是，赤潮的发生和消失突然，持续时间短，一般不超过３ｄ。  相似文献   

柴达木盆地昆特依盐湖的地球化学演化与古气候变化   总被引：9，自引：1，他引：9  

韩凤清  黄麒  王克俊  王华安  原力 《海洋与湖沼》1995,26(5):502-508

１９９０年用铀系年代学及古地磁对昆特依盐湖沉积物进行年代测定，探讨该湖区地球化学演化与古气候变化关系。结果表明，该区最老的盐层形成于早更新世晚期（大于７３０ｋａ），大量的盐类沉积则始于３００ｋａ左右；盐湖沉积物中元素含量变化主要受古盐度、矿物、类质同象和吸附控制；沉积物水溶Ｆ／Ｃｌ比值与古盐度成反比关系；该湖２０００ｋａ以来的古环境演变：盐层形成于干冷、偏酸性的氧化环境，泥岩层形成于湿暖、偏碱性的  相似文献   

Representation of double-peaked sea wave spectra in Jiaozhou Bay   总被引：1，自引：0，他引：1  

Huang Peiji  Hu Zejian 《海洋学报(英文版)》1989,8(1):41-50

The spectrum shape characteristics of double-peaked sea wave spectia actually measured in the Jiaozhou Bay were analysed and one representation of double-peaked sea wave spectra with six-parameters which can be determined from a given sea wave spectrum is proposed in this paper. After verifying by fitting process with the data sets of double-peaked sea wave spectra measured in the Jiaozhou Bay, it can be seen from the results that the representation mentioned above may be used to express the double-peaked sea wave spectra generated in the Jiaozhou Bay under various sea states, and that the absolute values of maximum deviation index (D.I.) do not exceed 30. 0.  相似文献   

Study of zooplankton ecology in Zhejiang coastal upwelling system-Zooplankton biomass and abundance of major groups   总被引：1，自引：0，他引：1  

He Dehu  Yang Guanming  Fang Shaojin  Shen Weilin  Liu Hongbin  Gao Aigen  Huang Shuxin 《海洋学报(英文版)》1988,7(4):607-620

The studied area is a shallow water area that belongs to the East China Sea continent shelf. The distribution of zooplankton biomass is higher inshore than offshore. The maximum abundance is in the inshore edge of the centre of upwelling, which is the superposed area of the salinity front and temperature front, due to the fact that the mixture of three different waters has brought about a concentration of nutrients. The herbivorous Euphausia, Copepoda and Tunicata are the major groups of the abundant area. Tunicata possess the possibility to compete against other kinds of herbivorous zooplankton. This means that there is negative correlation between the distribution of Tunicata and that of Copepoda and Euphausia. The positions of maximum areas of zooplankton biomass, phytoplankton individual and the concentration of phosphates and chlorophyll-a overlap one another. In the centre of upwelling, zooplankton can not adapt itself to the environment of lower temperature and less oxygen even with rich nutrient  相似文献   

A New Approach to High-Order Boussinesq-Type Equations with Ambient Currents   总被引：6，自引：3，他引：6  

王亚玲张洪生  缪国平朱良生 《中国海洋工程》2005,19(1):49-60

A new approach to high-order Boussinesq-type equations with ambient currents is presented. The current velocity is assumed to be uniform over depth and of the same magnitude as the shallow water wave celerity. The wave velocity field is expressed in terms of the horizontal and vertical wave velocity components at an arbitrary water depth level. Linear operators are introduced to improve the accuracy of the kinematic condition at the sea bottom. The dynamic and kinematic conditions at the free surface are expressed in terms of wave velocity variables defined directly on the free surface. The new equations provide high accuracy of linear properties as well as nonlinear properties from shallow to deep water, and extend the applicable range of relative water depth in the case of opposing currents.  相似文献   

On the recovery of surface wave by pressure transfer function     

Cheng-Han Tsai  Min-Chih Huang  Fu-Ji Young  Yin-Chern Lin  Hsien-Wen Li 《Ocean Engineering》2005,32(10):4936-1259

Except the commonly selected pressure transfer function derived from the linear wave theory, a previous study on the pressure transfer function for recovering surface wave from underwater pressure transducer suggested that the pressure transfer function is a function of frequency parameter only. With careful analysis, this study showed that the pressure transfer function should include a transducer submergence parameter as that given by the linear theory. It was found that the previously suggested empirical formula should be restricted to measurements with the pressure transducer close to the surface; otherwise overestimation of wave height would result. Field measurements were carried out with an acoustic wave gauge and a synchronized pressure transducer located at various depths with submergence parameter close to 1 (near the sea floor). It was shown that the previous one-parameter empirical formula might overestimate the significant wave height by more than 30%. This study found that with deep-water wave bursts excluded, the transfer function based on the linear wave theory provided a fairly good estimation on the significant wave heights, with an average deviation of 3.6%.  相似文献   

Distribution of dissolved organic carbon in and near the Prydz Bay, Antarctica     

Qiu Yusheng  Chen Min  Huang Yipu  Liu Guangshan 《海洋学报(英文版)》2003,22(4):547-556

INTRODUCTIONDissolvedorganiccarbon (DOC)makesupthesecondlargestofthebioactivepoolsofcar bonintheocean ,secondtothelargestpoolofdissolvedinorganiccarbon .Theglobaldissolvedorganiccarbonpoolisestimatedtobe 6 85Gt,avaluecomparabletothemassofCO2 intheat mosphere (Hedges,1 992 ) .Thesizeofthereservoir,aswellasitsdynamics ,indicatesthatDOCplaysacentralroleintheoceancarboncycle .AsitrelatestogreenhousegasessuchasCO2 andassociatesclimatecycle ,oceanicDOCbiogeochemicalcycleshavebeenoneoftheh…  相似文献