固定化鲅鱼乙酰胆碱酯酶的制备及部分性质测定   总被引：3，自引：0，他引：3  

孟范平  何东海  朱小山  马冬冬  杨正先  YANG Zheng-Xian 《中国海洋大学学报(自然科学版)》2005,35(6):1067-1071

酶的固定化是酶传感器制备过程中重要的1个环节.研究采用直接共价法固定鲅鱼乙酰胆碱酯酶（AChE）.制备方法为：0.1g CNBr活化的Sepharose 4B凝胶用1mmol/L的HCl充分溶胀后,与活力为10U的AChE溶液混合,于4℃下150r/min振荡8h.所制备的固定化酶活力回收率较高（96%）,对pH值和温度变化的适应能力均优于非固定化酶;在3个月保存期内,前者的活力损失13%,而后者的活力则下降89%.这说明固定过程能够大大提高AChE的抗逆性和保持酶活力的稳定,有利于酶传感器的制备.  相似文献   

Stability Design System of Submarine Pipelines     

Gao Yinli Yan Shuwang Wang Jinying Graduate Student  Tianjin University  Tianjin Professor  Tianjin University  Tianjin Senior Engineer  China Offshore Oil Engineering Co. 《中国海洋工程》1995,(1)

This paper introduces a stability design system of submarine pipelines. The system can provide a reasonable design procedure for users with reference to domestic and foreign methods and in the light of home engineering experience.  相似文献   

渤海湾沿岸泥炭沼泽形成机制与时空分布   总被引：4，自引：0，他引：4  

李汉鼎  王升忠 《海洋地质与第四纪地质》1995,15(2):11-16

渤海湾沿岸的海陆过渡带，优越的水文地理环境有利于泥炭沼泽的形成与发育。海洋海退导致的海岸线变迁，是控制泥炭沼泽发育时间和空间分布的主导因素。间冰期和冰后期的海面上升期间，自海向陆发育了海侵基底泥炭；中全新世高海面期，在山前扇缘洼地和沟谷有泥炭沼泽发育；晚全新世海面下降期，泥炭沼泽主要发育在沿岸的海湾、泻湖、三角洲汊河间洼地及平原上的河滩、阶地、古河道等地。  相似文献   

Relationship between biogeochemical features of biogenic elements and flocculation in the Changjiang Estuary   总被引：1，自引：3，他引：1  

Lin Yi''an  Tang Renyou  Li Yan  Dong Henglin  Guan Xuwei  Chen Yinzu 《海洋学报(英文版)》1995,14(2):225-234

ＲｅｌａｔｉｏｎｓｈｉｐｂｅｔｗｅｅｎｂｉｏｇｅｏｃｈｅｍｉｃａｌｆｅａｔｕｒｅｓｏｆｂｉｏｇｅｎｉｃｅｌｅｍｅｎｔｓａｎｄｆｌｏｃｃｕｌａｔｉｏｎｉｎｔｈｅＣｈａｎｇｊｉａｎｇＥｓｔｕａｒｙ￥ＬｉｎＹｉ＇ａｎ；ＴａｎｇＲｅｎｙｏｕ；ＬｉＹａｎ；Ｄｏ...  相似文献   

A New Approach to High-Order Boussinesq-Type Equations with Ambient Currents   总被引：6，自引：3，他引：6  

王亚玲张洪生  缪国平朱良生 《中国海洋工程》2005,19(1):49-60

A new approach to high-order Boussinesq-type equations with ambient currents is presented. The current velocity is assumed to be uniform over depth and of the same magnitude as the shallow water wave celerity. The wave velocity field is expressed in terms of the horizontal and vertical wave velocity components at an arbitrary water depth level. Linear operators are introduced to improve the accuracy of the kinematic condition at the sea bottom. The dynamic and kinematic conditions at the free surface are expressed in terms of wave velocity variables defined directly on the free surface. The new equations provide high accuracy of linear properties as well as nonlinear properties from shallow to deep water, and extend the applicable range of relative water depth in the case of opposing currents.  相似文献   

Variation of reactivity of particulate and sedimentary organic matter along the Zhujiang River Estuary   总被引：3，自引：0，他引：3  

Chen Jianfang  Jin Haiyan  Yin Kedong  Li Yan 《海洋学报(英文版)》2003,22(4):557-568

To investigate organic matter source and reactivity in the Zhujiang River (Pearl River)Estuary and its adjacent areas, particulate organic carbon (POC), particulate hydrolysable amino acids (PHAA), and Chl a during two cruises in July 1999 and July 2000 were measured. The highest POC and PHAA concentration was observed in the waters with maximum Chl a. The spectra distribution,relative content (dry weight in milligram per gram), PHAA-C% POC and other indicators such as the ratios of amino acids vs. amino sugars (AA/AS) and glucosamine vs. galactosamine (Glum/Gal) suggested that particulate amino acids in the water column and sediments in the Zhujiang River Estuary were mainly derived from biogenic processes rather than transported from terrestrial erosion. In inner estuary where high turbidity was often observable, organic matter was mainly contributed by re-suspension of bottom sediments with revealed zooplankton, microbial reworked characteristics, which suggest that these organic matters were relatively “old“. In the estuarine brackish region, organic matter in water column is mainly contributed by relatively fresh, easily degradable phytoplankton derived organic matter.During physical - biological processes within the eastuary, organic matter derived from phytoplankton was subjected to alteration by zooplankton grazing and bacterial reworking.  相似文献   

海洋声层析的基本原理和应用     

朱芸  吕连港 《海洋科学进展》2002,20(4):70-75

从射线声学和简正波声学的角度，概述了海洋声层析的基本理论，包括射线走时反演、简正波走时反演、简正波相位反演和简正波水平折射层析。海洋声层析以反演海水温度和流速为基础。还总结了声层析在海洋学研究中的应用。  相似文献   

筒型基础沉贯阻力估算方法   总被引：1，自引：0，他引：1  

何炎平 《海洋工程》2002,20(3):23-28

系统地总结国外有代表性工程实践对筒型基础沉贯阻力的研究 ,并将各种方法综合起来 ,提出了加权综合估算法 ,供今后的研究和工程实践参考  相似文献   

An Approach to Stability Analysis of Embedded Large-Diameter Cylinder Quay   总被引：3，自引：3，他引：3  

WANG  Yuanzhan 《中国海洋工程》2002,16(3):383-393

The large-diameter cylinder structure, which is made of large successive bottomless cylinders placed on foundation bed or partly driven into soil, is a recently developed retaining structure in China. It can be used in port, coastal and off-shore works. The method for stability analysis of the large-diameter cylinder structure, especially for stability analysis of the embedded large-diameter cylinder structure, is an important issue. In this paper, an idea is presented that is, em-bedded large-diameter cylinder quays can be divided into two types, i.e. the gravity wall type and the cylinder pile wall type. A method for stability analysis of the large-diameter cylinder quay of the cylinder pile wall type is developed and a method for stability analysis of the large-diameter cylinder quay of the gravity wall type is also proposed. The effect of sig-nificant parameters on the stability of the large-diameter cylinder quay of the cylinder pile wall type is investigated through numerical calculation.  相似文献   

冲绳海槽中段沉积岩芯碳酸盐和烧失量的古环境意义     

吴世迎  刘焱光  王湘芹  白黎明  吴军瑞 《海洋科学进展》2001,19(2):17-24

对冲绳海槽中段 3个沉积岩芯碳酸盐和烧失量的系统分析得知 ,岩芯沉积物的CaCO3含量一般 >1 0 % ,最高含量接近 30 % ,烧失量大都在 1 0 %～ 30 %之间变化 ,反映了该海区处于溶跃面之上钙质生物极为发育的生物地球化学环境。结合δ1 8O曲线所反映的气候环境变化 ,探讨了 1 2 0kaB .P .以来碳酸盐旋回的“大西洋型”演变特点 ,同时对该区沉积地层中的火山热液活动印记作了一定的探讨。  相似文献