抗凝血酶的结构、功能、表达和演化     

晁叶青  张士璀 《海洋科学》2008,32(7):71-74

包括人在内的哺乳动物凝血系统参与止血反应,是机体一种独特的保护性机能。凝血与溶血处于动态平衡状态,是一个精密控制的过程。血管破损血液必须快速准确地在破损处凝集,阻止血液外流。如果止血反应不够迅速,将血流不止,危及生命。相反,如果止血反应一直进行下去,也会导致血栓,同样危及生命。血凝过程是一个复杂的级联过程,参与反应的多种物质被称为凝血因子,其主要成分是蛋白质。  相似文献   

对虾血淋巴脂蛋白的生化特征及分子克隆研究进展     

李晓华  王宝杰  王雷  熊冬金  刘??梅  蒋克勇 《海洋科学》2008,32(11):80-83

脂类是海洋无脊椎动物包括对虾能量的主要来源,脂类在其生长发育的很多重要过程中起作用,如蜕皮和繁殖。虾蟹及鱼类优先使用脂类作为能源,因此,它们的血淋巴脂蛋白水平较高。脂蛋白是对虾体内一种重要的蛋白质,其主要作用是：参与组织或细胞间脂类的运输和重分配;参与免疫防御反应过程;能与细胞膜上的特异受体结合,影响脂类摄取。  相似文献   

细菌逃避补体识别和攻击的机制     

LI Zhi-Min  张士璀 《中国海洋大学学报(自然科学版)》2008,38(4)

补体作为和适应性免疫存在联系的先天性免疫系统组成部分,在抵制细菌感染中起着重要作用.近一个多世纪关于细菌和补体系统的平行研究,致病菌逃避补体介导的调理吞噬及直接杀伤的基本机理已经为人们所了解.特别是近二、三十年来,有关细菌逃避补体识别和攻击机理研究取得一些新进展,结合作者近几年来相关研究,对此做了简要评述.  相似文献   

LABORATORY OBSERVATION ON SPAWNING,FECUNDITY AND LARVAL DEVELOPMENT OF AMPHIOXUS (BRANCHIOSTOMA BELCHERI TSINGTAUNESE)   总被引：2，自引：0，他引：2  

吴贤汉  张士璀  王永元  张宝禄  曲艳梅  江新霁 《中国海洋湖沼学报》1994,(4)

Although amphioxus is widespread in temperate and tropical seas,its population is diminishing be-cause of environmental pollution.To keep the population of this evolutionarily important animal fromdiminishing,study on its reproduction and development is necessary.The main findings in this study onthe spawning and fecundity of the amphioxus reared in laboratory and its larval development are as fol-lows.1.Water temperature markedly affected the spawning.It spawned only when water temperature reached 21℃.2.Spawning of the amphioxus in laboratory was markedly extended.Initially,the amphioxus spawned at about 7:00 PM,but spawning time was postponed as spawning days went on.3.The number of eggs produced by a female ranged from 1400 to 12800,average of 5800.This al-so represents the fecundity of the amphioxus because it shedded all eggs within the ovary at a time.4.During the first few months of life of the amphioxus,its growth rate changed seasonally.Thegrowth rate in summer and fall was greater than that in  相似文献   

LOCALIZATION OF A PUTATIVE EPIBOLY-DETERMINING FACTOR IN OOCYTES OF THE GOLDFISH (CARASSIUS AURATUS)     

张士璀  吴贤汉 《中国海洋湖沼学报》1996,(3)

It is unknown whether cytoplasmic determinants in goldfish eggs are asymmetrically localized beforematuration. In this study fully grown goldfish oocytes with infact germinal vesicles (GVs) were ligated withbaby hair loops along desired planes into two parts, mature in vitro, and inseminated. Embryos developedfrom the animal halves with GV of oocytes ligated along the equator un unable to undergoepiboly, while those developed from the GV-containing parts of oocytes ligated along the animal-vegetal axiswere able to. Also, embryos developed from the animal halves obtained from fertilized eggs could undergo epiboly. From there results, it was postulated that the goldfish's premature oocyte possessed a putativefactor in the vegetal hemisphere which was responsible for epiboly of the embryonic cells.  相似文献   

IN VITRO STUDY ON CYTOTOXIC EFFECTS OF THE ORGANOPHOSPHOROUS PESTICIDE PROFENOFOS ON THE GILL CELL LINE,FG-9307, OF THE FLOUNDER （PARALICHIHYS OLIVACEUS）   总被引：2，自引：0，他引：2  

李红岩  张士璀姜明 汪岷 《中国海洋湖沼学报》2001,19(1):57-62

The FG-9307 cell line derived from the gill of flounder Paralichthys olivaceus was used in the present study to determine the acute cytotoxic effects of the organophosphorous pesticide, profenofos. It was found that the cell growth rate was markedly reduced by profenofos at the concentrations of 2.5, 5 and 7.5 mg/L tested; and that the fine structures of the cells were also altered by profenofos, as evidenced by dilation of nuclear membranes and mitochondria cristae, and presence of enlarged lysoscrneswith engulfed organelles and numerous vacuoles in the cytoplama. Probably, mitochondria, the cell ener-gy-generating sites, are the most prcrninent sites of profenofos cytotoxity in the cells. This seems to be the first report of the use of marine fish cell line for evaluation of the acute in vitro cytotoxicity of organophos-phorus pesticide.  相似文献   

IN VITRO STUDY ON CYTOTOXIC EFFECTS OF THE ORGANOPHO SPHOROUS PESTICIDE PROFENOFOS ON THE GILL CELL LINE, FG-9307, OF THE FLOUNDER (PARALICHTHYS OLIVACEUS)     

李红岩  张士璀  姜明  汪岷 《中国海洋湖沼学报》2001,(1)

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In vivo effects of 17-β-estradiol on plasma immunoglobulin levels and leukocyte density in zebrafish Danio rerio     

李磊  张士璀  仝舟  刘佳 《中国海洋湖沼学报》2010,(3)

Estradiol, or 17-β-estradiol (E2), the most potent naturally occurring estrogen, is involved in the hormone-immune system interaction in both mammals and fish. However, in vivo studies are largely limited, and little is known about whether E2 exerts similar effects on both female and male zebrafish (Danio rerio). Here, we show exposure of both sexes of D. rerio to 20 nmol/L E2 resulted in a significant increase in Vg1 expression, but caused little damage to the hepatocytes, suggesting that this is the optimum E2 concentration. Also, exposure to 20 nmol/L E2 for 20 days caused a marked increase in plasma IgM levels, but had little influence on the peripheral leukocyte density, providing the first evidence of a hormone-immune system interaction in this species.  相似文献   

毛蚶血清凝集素的凝集活性   总被引：2，自引：1，他引：2  

刘志鸿  张士璀  杨爱国  王长法  王清印 《海洋学报》2003,25(5):92-96

以毛蚶为实验材料,对其血清中的凝集素进行研究.毛蚶血清凝集素对人的A,B,O血型红细胞、鸡和小鼠的红细胞均有凝集作用,凝集效价分别为256,256,8,16,64,同时它还能对白色念珠菌、啤酒酵母、溶藻胶弧菌等微生物具有凝集作用.毛蚶血清凝集素具有一定的pH值稳定性和热稳定性.在选用的12种单糖和寡糖中,L-鼠李糖、L-树胶醛糖、麦芽糖、D-半乳糖、蔗糖对毛蚶血清的凝集活性有明显的抑制作用.用弧菌对毛蚶进行免疫刺激时,其血清对鸡红细胞的凝集效价明显增加.  相似文献   

凹顶藻属海藻化学成分研究进展   总被引：2，自引：0，他引：2  

史大永  贺?? 娟  许?? 凤  袁兆慧  范?? 晓  石建功  韩丽君 《海洋科学》2007,31(4):81-91

海洋生物通过生物链、化学信息传送、化学进攻、化学防御等机制相互依存、相对独立、维系着独特紧密的海洋生物体系,作为海洋物质代谢原动力的海藻为了对抗食藻动物的吞食,大多能产生一些有特色的代谢物质,其中凹顶藻属因富含萜类化合物著称,这类化合物含有异戊二烯结构单元,不  相似文献