对虾一种无包涵体杆状病毒病原的PCR检测   总被引：4，自引：0，他引：4  

石拓  孔杰  刘萍  包振民  韩玲玲  徐怀恕 《海洋学报》2000,22(4):96-100

为建立对虾无包涵体杆状病毒的快速诊断技术,应用PCR技术分别对暴发性流行病发生前、流行中和发病后的对虾样品进行了检测.根据已克隆的对虾杆状病毒部分基因组序列而设计的引物能特异性地扩增出靶DNA片段,最低可以检测1pg的病素DNA.由典型发病症状对虾的胃、腮、肝胰腺、中肠、游泳足、肌内和心脏等器官和组织中成功地检测到病毒.不同组织和器官经扩增得到的信号强弱不同:病虾的胃扩增得到的信号最强,中肠、肌肉、心脏和游泳足次之,腮和肝胰腺信号最弱,说明病毒在不同组织和器官中数量及感染程度不同.在对虾发病前及发病后,用二次PCR还能检测表面不发病呈隐性感染状态的对虾携带的病毒;而对野生健康虾的检测结果为阴性.研究表明,PCR是检测对虾暴发性流行病快速、灵敏而准确的方法,对病毒病的早期诊断、防治和高健康对虾品种的选育具有指导意义.  相似文献   

冲绳海槽海底地形的补偿模式初步研究   总被引：1，自引：1，他引：1  

刘保华  刘忠臣 《海洋地质与第四纪地质》1998,18(4):29-34

从区域补偿模式和实验均衡理论出发,利用重力和地形资料计算了冲绳海槽的均衡响应函数,结果表明：冲绳海槽南段弹性板有效厚度和补偿深度明显大于中段,结合其它地质地球物理资料解释认为,产生这种差异的原因主要是南,中两段岩石圈温度和补偿机制的不同所致。  相似文献   

Sea surface height anomaly and geostrophic circulation variations in the South China Sea from TOPEX/POSEIDON altimetry     

Liu Kexiu  Ma Jirui  Xu Jianping  Han Guijun  Fan Zhenhua 《海洋学报(英文版)》2002,21(3):345-354

The sea surface height anomaly (SSHA) and geostrophic circulation in the South ChinaSea (SCS) are studied using TOPEX/POSE1DON (T/P) altimetry data. The SSHA, which is obtained after tidal correction based on the tidal results from T/P data, is predominated by seasonal alternating monsoons. The results reveal that the SSHA in the central part of the SCS is positive in spring and summer, but negative in autumn and winter. It is also found that the SSHA in the SCS can be approached with the sum of tidal constituents SA and SSA. The geostrophic circulations in the SCS are calculated according to sea surface dynamic topography, which is the sum of SSHA and mean sea surface height. It is suggested that the circulation in the upper layer of the SCS is generally cyclonic and notably western intensified during autumn and winter, while the western intensification is weak during spring and summer. It is also indicated that the Kuroshio intrudes into the northeastern SCS throuth the Luzon Strait in winter. But ther  相似文献   

Measurement of ²²⁴Ra, ²²⁶Ra and ²²⁸Ra in seawater using Mn-fiber adsorption-γ spectrum method   总被引：2，自引：1，他引：2  

Liu Guangshan  Huang Yipu  Chen Xinbao 《海洋学报(英文版)》2000,19(2):19-27

I~IOXThe half-lives of natural radioactive nuclides "'Ra, 226Ra and 228Ra are 3. 66 d, 5. 76 a,1600 a respectively, hence, the marine process with various time scales can be researched by taking these nuclides as tracers. Because these nuclides can be determined by measuring them or theirdaughters' a,g, y-rays, they have been applied extensively in oceanography research. Huang(1996), Huang et al. (1996 a,b) and Chen (1996) have given reviews on it.Since the contents of "'Ra, 226Ra and 228…  相似文献   

A dipole mode at thermocline layer in the tropical Indian Ocean     

Tian Jiwei  Liu Huiqing  Qian Weihong 《海洋学报(英文版)》2003,22(1):15-24

Temperature data at different layers of the past 45 years were studied and we found adiploe mode in the thermocline layer (DMT): anomalously cold sea temperature off the coast of Sumatra and warm sea temperature in the western Indian Ocean. First, we analyzed the temperature and the temperature anomaly (TA) along the equatorial Indian Ocean in different layers. This shows that stronger cold and warm TA signals appeared at subsurface than at the surface in the tropical Indian O-cean. This result shows that there may be a strong dipole mode pattern in the subsurface tropical Indian Ocean. Secondly we used Empirical Orthogonal Functions (EOF) to analyze the TA at thermocline layer. The first EOF pattern was a dipole mode pattern. Finally we analyzed the correlations between DMT and surface tropical dipole mode (SDM), DMT and Nino 3 SSTA, etc. and these correlations are strong.  相似文献   

Cyclone formation and development in the Antarctic Prydz Bay     

Xie Simei  Mei Shan  Liu Kewei  Wei Lixin 《海洋学报(英文版)》2002,21(1):45-54

Using meteorological data of field observation in 1990 - 2000, especially polar orbit high-resolution NOAA satellite cloud maps received from the Antarctic expedition vessel since 1997, the formation and development of the Prydz Bay cyclone are studied in this paper. Some new viewpoints are suggested such as: when surround-polar cyclone enters the Prydz Bay, it can also intensify and develop in summer; cyclone can also develop in the easterlies in this bay. These view points revise old uncom-plete view point that the Prydz Bay is a burial ground of cyclone, and also further consummate formation-development theory of surround-cyclone in the Antarctic westerlies and cyclone in the Antarctic easterlies. In this paper, the mechanism of ice-air-sea interaction in the Prydz Bay is studied, and the physical process of cyclone formation-development is explained. By use of wholly dynamic transportation method, an energy exchange case of a cyclone, which explosively developed after entering the Prydz Bay, is calcu  相似文献   

Measurement of nuclides of uranium and thorium series of disequilibrium using γ-spectroscopy   总被引：3，自引：0，他引：3  

Liu Guangshan  Huang Yipu  Li Jing  Ye Lin 《海洋学报(英文版)》2002,21(4):505-517

ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅｒａｄｉｏｎｕｃｌｉｄｅｓｏｆｎａｔｕｒａｌｕｒａｎｉｕｍａｎｄｔｈｏｒｉｕｍｓｅｒｉｅｓｃａｎｂｅｕｓｅｄｔｏｔｒａｃｅｍａｒｉｎｅｐｒｏ ｃｅｓｓｏｆｖａｒｉｏｕｓｔｅｍｐｏｒａｌａｎｄｓｐａｔｉａｌｓｃａｌｅａｎｄｐｒｏｐｅｒｔｉｅｓｔｈａｔｐｒｏｖｉｄｅｐｌｅｎｔｉｆｕｌｉｎｆｏｒｍａｔｉｏｎａｂｏｕｔｐｈｙｓｉｃｓ,ｃｈｅｍｉｓｔｒｙ ,ｇｅｏｓｃｉｅｎｃｅａｎｄｂｉｏｌｏｇｙｏｆｏｃｅａｎｂｅｃａｕｓｅｔｈｅｙｈａｖｅｄｉｆｆｅｒｅｎｔｃｈｅｍｉｃａｌｐｒｏ…  相似文献   

Variation in Water Masses on Shelf of Northern Bering Sea in September 2016-2018     

Yingjie Wang  Na Liu  Lina Lin  Yan He  Bin Kong  Zhanhai Zhang 《Marine Geodesy》2020,43(3):285-301

Abstract

Based on hydrological data obtained during the 7th to 9th Chinese National Arctic Research Expeditions in the summers of 2016–2018, the main water structure on the shelf of the northern Bering Sea and the volume and heat fluxes of the Bering Strait throughflow were analyzed. Distinct variability was identified in the three Pacific water masses feeding the strait - Anadyr Water (AW), Bering Shelf Water (BSW) and Alaskan coastal water (ACW). Overall, the temperature and salinity of the entire section increased each year, with 2018 showing significant anomalies, i.e., a temperature anomaly of up to 1?°C and a maximum salinity anomaly of 2. From 2016 to 2018, the extent of the ACW gradually narrowed in the eastern part of this section, while the AW expanded eastward each year. The net volume transport through each of the three sections increased poleward from 1.65?Sv to 2.76?Sv, with the AW increasing from 0?Sv to 1.03?Sv, the BSW varying between 0.52–1.65?Sv, and the ACW gradually decreasing from 1.04?Sv to disappearing completely. The net heat fluxes were also poleward, varying between 25.77 TW and 61.50 TW, and showing a significant increase. Significant variations in magnitude and extent were observed in each water mass of the Bering Strait throughflow, which could produce widespread effects in the Arctic Ocean and the global ocean beyond.  相似文献   

A semi-analytical solution for random wave-induced soil response and seabed liquefaction in marine sediments     

Haijiang Liu  Dong-Sheng Jeng   《Ocean Engineering》2007,34(8-9):1211-1224

In this study, unlike most previous investigations for wave-induced soil response, a simple semi-analytical model for the random wave-induced soil response is established for an unsaturated seabed of finite thickness. Two different wave spectra, the B-M and JONSWAP spectra, are considered in the new model. The influence of random wave loading on the soil response is investigated by comparing with the corresponding representative regular wave results through a parametric study, which includes the effect of the degree of saturation, soil permeability, wave height, wave period and seabed thickness. The maximum liquefaction depth under the random waves is also examined. The difference on the soil response under the two random wave types, B-M and JONSWAP frequency spectra, is also discussed in the present work.  相似文献   

A note on the effects of a thin visco-elastic mud layer on small amplitude water-wave propagation     

Philip L.-F. Liu  I-Chi Chan 《Coastal Engineering》2007

In this note we investigated the effects of a thin visco-elastic mud layer on wave propagation. Within the framework of linear water-wave theory, analytical solutions are obtained for damping rate, dispersion relation between wave frequency and wave number, and velocity components in the water column and mud layer. The wave attenuation rate reaches a maximum value when the mud layer thickness is about the same as the mud boundary layer thickness. Heavier mud has a weaker effect on the wave damping. However, the wave attenuation rate does not always decrease as the elastic shear modulus increases. In the range of small values for elastic shear modulus, the wave attenuation can be amplified quite significantly. The current solutions are compared with experimental data with different wave conditions and mud properties. In general, good agreements are observed.  相似文献