A new method to detect viable bacteria in natural seawater using 16SrRNA oligonucleotide probe     

Masahiko Nishimura  Kumiko Kita-Tsukamoto  Kazuhiro Kogure  Kouichi Ohwada  Usio Simidu 《Journal of Oceanography》1993,49(1):51-56

Fluorescent oligonucleotide probes were used to detectVibrio cholerae directly under epifluorescence microscope. The probe which is complementary to the specific 16SrRNA sequence ofVibrio cholerae was labelled with Texas-Red, whereas the universal probe was labelled with Fluorescein. These probes allowed the distinctionVibrio cholerae from other eubacteria under the same microscopic field. In order to detect and enumerate specific bacteria in natural seawater, this method was combined with the direct viable count (DVC) technique. The combined method increased intracellular rRNA levels in the sample, and made it possible to detect the target bacteria with the specific gene probe. The applicability of this new method was confirmed both for the laboratory mixed culture system and natural seawater.  相似文献   

The Nobeyama Millimeter Array Survey for protoplanetary disks around protostar candidates and T Tauri stars in Taurus     

Nagayoshi Ohashi  Ryohei Kawabe  Masato Ishiguro  Masahiko Hayashi 《Astrophysics and Space Science》1994,212(1-2):239-250

The Nobeyama Millimeter Array Survey for protoplanetary disks has been made for 19 protostellar IRAS sources in Taurus; 13 of them were optically invisible protostars and 6 were young T Tauri stars. We observed 98-GHz continuum and CS(J = 2 – 1) line emissions simultaneously with spatial resolutions of 2 _. 8-8 _. 8 (360-1,200 AU). The continuum emission was detected from 5 out of 6 T Tauri stars and 2 out of 13 protostar candidates: the emission was not spatially resolved and was consistent with being originated from compact circumstellar disks. Extended CS emission was detected around 2 T Tauri stars and 11 protostar candidates. There is a remarkable tendency for the detectability of the 98-GHz continuum emission to be small for protostar candidates. This tendency is explained if the mass of protoplanetary disks around protostars is not as large as that around T Tauri stars; the disk mass may increase with the increase of central stellar mass by dynamical accretion in the course of evolution from protostars to T Tauri stars.Paper presented at the Conference onPlanetary Systems: Formation, Evolution, and Detection held 7–10 December, 1992 at CalTech, Pasadena, California, U.S.A.  相似文献   

Redistribution and export of contaminated sediment within eastern Fukushima Prefecture due to typhoon flooding          下载免费PDF全文

Akihiro Kitamura  Hiroshi Kurikami  Kazuyuki Sakuma  Alex Malins  Masahiko Okumura  Masahiko Machida  Koji Mori  Kazuhiro Tada  Yasuhiro Tawara  Takamaru Kobayashi  Takafumi Yoshida  Hiroyuki Tosaka 《地球表面变化过程与地形》2016,41(12):1708-1726

Tropical cyclones expose river basins to heavy rainfall and flooding, and cause substantial soil erosion and sediment transport. There is heightened interest in the effects of typhoon floods on river basins in northeast Japan, as the migration of radiocaesium‐bearing soils contaminated by the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident will affect future radiation levels. The five main catchments surrounding FDNPP are the Odaka, Ukedo, Maeda, Kuma and Tomioka basins, but little quantitative modelling has been undertaken to identify the sediment redistribution patterns and controlling processes across these basins. Here we address this issue and report catchment‐scale modelling of the five basins using the GETFLOWS simulation code. The three‐dimensional (3D) models of the basins incorporated details of the geology, soil type, land cover, and used data from meteorological records as inputs. The simulation results were checked against field monitoring data for water flow rates, suspended sediment concentrations and accumulated sediment erosion and deposition. The results show that the majority of annual sediment migration in the basins occurs over storm periods, thus making typhoons the main vectors for redistribution. The Ukedo and Tomioka basins are the most important basins in the region in terms of overall sediment transport, followed by the other three basins each with similar discharge amounts. Erosion is strongly correlated with the underlying geology and the surface topography in the study area. A low permeability Pliocene Dainenji formation in the coastal area causes high surface water flow rates and soil erosion. Conversely, erosion is lower in an area with high permeability granite basement rocks between the Hatagawa and Futaba faults in the centre of the study area. Land cover is also a factor controlling differences in erosion and transport rates between forested areas in the west of the study area and predominantly agricultural areas towards the east. The largest sediment depositions occur in the Ogaki and Takigawa Dams, at the confluence of the Takase and Ukedo Rivers, and at the Ukedo River mouth. Having clarified the sediment redistribution patterns and controlling processes, these results can assist the ongoing task of monitoring radioactive caesium redistribution within Fukushima Prefecture, and contribute to the design and implementation of measures to protect health and the environment. Copyright © 2016 John Wiley & Sons, Ltd.  相似文献   

Rate-dependent strength of porous ice–silica mixtures and its implications for the shape of small to middle-sized icy satellites     

Minami Yasui  Masahiko Arakawa 《Icarus》2010,210(2):956-967

Porosity is one of the most important physical properties in the rheology of small icy satellites composed of ice–silicate mixtures. Deformation experiments involving ice and 1 μm silica bead mixtures were conducted to clarify the effect of porosity on the flow law of ice–silica mixtures. Mixtures with silica mass contents of 0, 30, and 50 wt.% were used for the experiments, and the porosity was changed from 0% to 25% in each mixture. The temperature ranged from −10 to −20 °C, and the strain rate was changed from 1.2 × 10⁻⁶ to 4.2 × 10⁻⁴ s⁻¹. As a result, it was found that the ice–silica mixtures deformed plastically, and that the relationship between the maximum stress, σ_max, on the stress–strain curve and the applied strain rate, , could be described by the following flow law: . The mixture became softer as the porosity or silica mass content increased, and the stress exponent n and activation energy Q were independent of porosity, depending only on the silica mass content. Furthermore, the parameter A₀ could be written as A₀ = B(1 − ?)^−α, where ? is the porosity. The constants B and α also depended only on the silica mass content, and they increased with the increase in this content. The Maxwell relaxation time was calculated in order to estimate the conditions for topographic relaxation of icy satellites, and it was found that topographic relaxation occurred at temperatures higher than 160 K in the case of icy satellites with mean radii of 200 km.  相似文献   

Experimental study on the collisional disruption of porous gypsum spheres     

Chisato Okamoto  Masahiko Arakawa 《Meteoritics & planetary science》2009,44(12):1947-1954

Abstract— In order to study the catastrophic disruption of porous bodies such as asteroids and planetesimals, we conducted several impact experiments using porous gypsum spheres (porosity: 50%). We investigated the fragment mass and velocity of disrupted gypsum spheres over a wide range of specific energies from 3 times 10³ J/kg to 5 times 10⁴ J/kg. We compared the largest fragment mass (m₁/M_t) and the antipodal velocity (V_a) of gypsum with those of non‐porous materials such as basalt and ice. The results showed that the impact strength of gypsum was notably higher than that of the non‐porous bodies; however, the fragment velocity of gypsum was slower than that of the non‐porous bodies. This was because the micro‐pores dispersed in the gypsum spheres caused a rapid attenuation of shock pressure in them. From these results, we expect that the collisional disruption of porous bodies could be significantly different from that of non‐porous bodies.  相似文献   

Characterization of drying-induced deformation behaviour of Opalinus Clay and tuff in no-stress regime   总被引：1，自引：0，他引：1  

Aung Ko Ko Soe  Masahiko Osada  Manabu Takahashi  Tai Sasaki 《Environmental Geology》2009,58(6):1215-1225

The drying-induced deformation behaviour of Opalinus Clay and tuff, which are being investigated under international and local collaborative projects for nuclear waste disposal in Switzerland and Japan, was investigated under a no-stress condition in the laboratory to evaluate their generic susceptibility to the formation of excavation damaged zone. The cylindrical core samples of Opalinus Clay and tuff were prepared to a one-dimensional drying condition and submitted to an uncontrolled laboratory environment. The strain evolution, evaporative water loss and environmental entities, such as temperature and relative humidity, were recorded simultaneously and quasi-continuously. It was observed that the drying phase induced significant strain magnitude and damage in Opalinus Clay samples, which was evidenced by the formation of hairy cracks on the surface parallel to the bedding. On the contrary, the strain occurrences in tuff samples were relatively insignificant, and no tendency of cracking was observed. In addition, the quasi-continuous availability of volumetric strains was further used in poroelastic relation for the estimation of capillary suction evolution. The calculated results were validated with pore size distributions obtained from mercury intrusion porosimetry.  相似文献   

High‐pressure polymorphs in Yamato‐790729 L6 chondrite and their significance for collisional conditions          下载免费PDF全文

Yukako Kato  Toshimori Sekine  Masahiko Kayama  Masaaki Miyahara  Akira Yamaguchi 《Meteoritics & planetary science》2017,52(12):2570-2585

Shock pressure recorded in Yamato (Y)‐790729, classified as L6 type ordinary chondrite, was evaluated based on high‐pressure polymorph assemblages and cathodoluminescence (CL) spectra of maskelynite. The host‐rock of Y‐790729 consists mainly of olivine, low‐Ca pyroxene, plagioclase, metallic Fe‐Ni, and iron‐sulfide with minor amounts of phosphate and chromite. A shock‐melt vein was observed in the hostrock. Ringwoodite, majorite, akimotoite, lingunite, tuite, and xieite occurred in and around the shock‐melt vein. The shock pressure in the shock‐melt vein is about 14–23 GPa based on the phase equilibrium diagrams of high‐pressure polymorphs. Some plagioclase portions in the host‐rock occurred as maskelynite. Sixteen different CL spectra of maskelynite portions were deconvolved using three assigned emission components (centered at 2.95, 3.26, and 3.88 eV). The intensity of emission component at 2.95 eV was selected as a calibrated barometer to estimate shock pressure, and the results indicate pressures of about 11–19 GPa. The difference in pressure between the shock‐melt vein and host‐rock might suggest heterogeneous shock conditions. Assuming an average shock pressure of 18 GPa, the impact velocity of the parent‐body of Y‐790729 is calculated to be ~1.90 km s^?1. The parent‐body would be at least ~10 km in size based on the incoherent formation mechanism of ringwoodite in Y‐790729.  相似文献   

A method of artificial generation of earthquake ground motion     

Masahiko Kimura  Masanori Izumi 《地震工程与结构动力学》1989,18(6):867-874

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Regional metamorphism and hydrothermal alteration related to Miocene submarine volcanism ('green tuff') in the Yurihara oil and gas field, northwest Honshu Island, Japan     

Masahiko  Yagi 《Island Arc》1993,2(4):240-261

Abstract Alteration of reservoir rocks in the Yurihara Oil and Gas Field, hereafter referred to as the ‘Yurihara field’, have been examined by using samples from six wells. These rocks are basalts in the lowermost part of the basin-fills (‘green tuff’ Formation). These basalts were produced in many eruptions in a submarine environment during the early to middle Miocene, and they underwent continuous intensive alteration genetically associated with Miocene submarine volcanism. The alteration of the basalts is of two types: low grade metamorphism and hydrothermal. The former belongs to the type of ocean floor metamorphism and comprises two subgroups: zeolite (zone I) and prehnite-pumpellyite (zones IIa: vein and amygdule occurrence, and IIb: replacing plagioclase). The latter is characterized by potassic metasomatism accompanied by adularia, quartz and calcite veins (zones IIIa: center and IIIb: margin of the metasomatism). This overprints the low grade metamorphic alteration. The central zone of hydrothermal alteration coincides with a major estimated fault, so that fluids probably assent along the fault. The basalts erupted during 16.5-15.5 Ma, determined by planktonic foraminifera assemblages of inter-bedded shales, then underwent successive low grade metamorphism. In time, the hydrothermal alteration that overprints low grade metamorphism occurred. Adularia veins of the altered rocks located in the hydrothermal alteration zones (zone IIIa and IIIb) have been dated as 9 Ma determined by the K-Ar method. This fact indicates that the activity of low grade metamorphism had already crossed the peak before hydrothermal alteration occurred at 9 Ma. The shape of isotherms of fluid inclusion homogenization temperatures (Th) and that of isolines of apparent salinity (Tm) almost coincide with each other, and these also coincide with the distribution of hydrothermal alteration (zones IIIa and IIIb). This indicates that the fluid inclusions formed at the same time as ascending fluids produced the potassic metasomatism. The maximum Th of the fluid inclusions is 222°C and Tm indicates trapped fluids of up to 3.3 wt% equivalent NaCl (i.e. almost the same as seawater). A Th versus Tm plot indicates mixing occurred between hydrothermal fluids and formation water that has low salinity. Corrensite and chlorite form veins, and the temperatures of their formation, estimated by the extent of aluminium substitution into the tetrahedral site of chlorite, ranges between 165 and 245°C in the centre of the hydrothermal alteration zone (zone IIIa). This is consistent with the result of Th analyses. The deposition temperature of chlorite associated with prehnite in veins ranges between 190 and 215°C in zones IIa and IIb.  相似文献   

Reducing hillslope size in digital elevation models at various scales and the effects on slope gradient estimation     

Chatchai Tantasirin  Masahiko Nagai  Taravudh Tipdecho  Nitin K. Tripathi 《国际地球制图》2016,31(2):140-157

Several models simulate watershed areas by delineating hillslopes. Hillslope size depends on the length of stream tributaries, which are affected by the drainage area threshold (DAT). There is no universal approach to identify the appropriate DAT. Therefore, a method to derive the DAT and a series of steps to delineate a watershed into smaller sizes were proposed in this study, and the impact of hillslope size on slope gradient estimation was investigated. The DAT obtained in this study was smaller than that obtained using other methods, resulting in a shorter length of the tributaries. Dividing these tributaries into equal short segments and using them to delineate the study area reduced the size of the hillslope. The results revealed that the shorter the length of the tributaries, the smaller the hillslope size. The accuracy of gradient estimation increased when the size of the hillslope was reduced.  相似文献