Isolation, distribution and evaluation of cytotoxic and antioxidant activity of cultivable actinobacteria from the Oman Sea sediments     

Gozari Mohsen  Bahador Nim  Jassbi Amir Rez  Mortazavi Mohammad Seddiq  Hamzehei Sam  Eftekhar Ebrahim 《海洋学报(英文版)》2019,38(12):84-90

Screening bioactive natural products from bacteria is a determinative step in the drug discovery programs. The present study aim to isolate actinobacteria from the Oman Sea sediments for determining the effects of different culture media and treatments on the yield of the isolation process, and measure the DPPH radical scavenging and Artemia cytotoxic activity of culture extracts of the actinobacterial isolates. A total of 290 actinobacterial isolates were collected from 14 sediment samples. Heat treatment(40.68%) and M_4 medium(29.31%) exhibited the maximum isolation rates of actinobacteria. Streptomyces isolates were dominantly distributed in all of the investigated stations according to 16 S rRNA gene sequencing. The distribution pattern of Streptomyces followed a depth-dependent frequency trend, whereas the members of rare genera including Micromonospora, Nocardia Actinoplanes, Nocardiopsis, Saccharopolyspora and Crossiella were distributed in deeper stations. Approximately,25% of the examined isolates could scavenge 90% of 10~(–4) mol/L DPPH solutions at 1 250 μg/mL final concentration of their ethyl acetate culture extracts. Furthermore, the most potent extracts could scavenge DPPH radicals with IC50 ranges from 356.8 to 566.4 μg/mL. Brine shrimp cytotoxicity tests showed that 38.88% of the examined culture extracts exhibited LC_(50) lower than 1 000 μg/mL against the Artemia cells. Moreover, the most potent culture extracts exhibited LC_(50) range from 335.4 to 534.4 μg/mL. Phylogenetic analysis by 16 S rRNA gene sequence revealed that the OS 005, OS 263 and OS 157 closely related to Streptomyces djakartensis, Streptomyces olivaceus and Nocardiopsis dassonvillei respectively. These results suggested the widespread distribution of the antioxidant and cytotoxic producing actinobacteria in the Oman Sea sediments, which could be considered as promising candidates for the discovery of microbial bioactive compounds.  相似文献   

Transient seepage analysis in zoned anisotropic soils based on the scaled boundary finite‐element method          下载免费PDF全文

Mohammad Hossein Bazyar  Abbas Talebi 《国际地质力学数值与分析法杂志》2015,39(1):1-22

The scaled boundary finite‐element method, a semi‐analytical computational scheme primarily developed for dynamic stiffness of unbounded domains, is applied to the analysis of unsteady seepage flow problems. This method is based on the finite‐element technology and gains the advantages of the boundary element method as well. Only boundary of the domain is discretized, no fundamental solution is required and singularity problems can be modeled rigorously. Anisotropic and non‐homogeneous materials satisfying similarity are modeled with no additional efforts. In this study, firstly, formulation of the method for the transient seepage flow problems is derived followed by its solution procedures. The accuracy, simplicity and applicability of the method are demonstrated via four numerical examples of transient seepage flow – three of them are available in the literature. Homogenous, non‐homogenous, isotropic and anisotropic material properties are considered to show the versatility of the technique. Excellent agreement with the finite‐element method is observed. The method out‐performs the finite‐element method in modeling singularity points. Copyright © 2014 John Wiley & Sons, Ltd.  相似文献   

Estimation of daily stream water temperatures with a Bayesian regression approach          下载免费PDF全文

Mohammad M. Sohrabi  Rohan Benjankar  Daniele Tonina  Seth J. Wenger  Daniel J. Isaak 《水文研究》2017,31(9):1719-1733

Stream water temperature plays a significant role in aquatic ecosystems where it controls many important biological and physical processes. Reliable estimates of water temperature at the daily time step are critical in managing water resources. We developed a parsimonious piecewise Bayesian model for estimating daily stream water temperatures that account for temporal autocorrelation and both linear and nonlinear relationships with air temperature and discharge. The model was tested at 8 climatically different basins of the USA and at 34 sites within the mountainous Boise River Basin (Idaho, USA). The results show that the proposed model is robust with an average root mean square error of 1.25 °C and Nash–Sutcliffe coefficient of 0.92 over a 2‐year period. Our approach can be used to predict historic daily stream water temperatures in any location using observed daily stream temperature and regional air temperature data.  相似文献   

Integration of aquifer geology,groundwater flow and arsenic distribution in deltaic aquifers – A unifying concept          下载免费PDF全文

Mohammad A. Hoque  William G. Burgess  Kazi M. Ahmed 《水文研究》2017,31(11):2095-2109

Groundwater arsenic (As) presents a public health risk of great magnitude in densely populated Asian delta regions, most acutely in the Bengal Basin (West Bengal, India and Bangladesh). Research has focused on the sources, mobilisation, and heterogeneity of groundwater As, but a consistent explanation of As distribution from local to basin scale remains elusive. We show for the Bengal Aquifer System that the numerous, discontinuous silt‐clay layers together with surface topography impose a hierarchical pattern of groundwater flow, which constrains As penetration into the aquifer and controls its redistribution towards discharge zones, where it is re‐sequestered to solid phases. This is particularly so for the discrete periods of As release to groundwater in the shallow subsurface associated with sea level high‐stand conditions of Quaternary inter‐glacial periods. We propose a hypothesis concerning groundwater flow ( S ilt‐clay layers I mpose H ierarchical groundwater flow patterns constraining A rsenic progression [SIHA]), which links consensus views on the As source and history of sedimentation in the basin to the variety of spatial and depth distributions of groundwater As reported in the literature. SIHA reconciles apparent inconsistencies between independent, in some cases contrasting, field observations. We infer that lithological and topographic controls on groundwater flow, inherent to SIHA, apply more generally to deltaic aquifers elsewhere. The analysis suggests that groundwater As may persist in the aquifers of Asian deltas over thousands of years, but in certain regions, particularly at deeper levels, As will not exceed low background concentrations unless groundwater flow systems are short‐circuited by excessive pumping.  相似文献   

A study of crude oil‐degrading bacteria from mangrove forests in the Persian Gulf     

Iman Fakhrzadegan  Mehdi Hassanshahian  Majid Askari Hesni  Amir Saadatfar 《Marine Ecology》2019,40(2)

Mangroves are coastal ecosystems, found in tropical and subtropical regions around the world. They are found in the transitional zones between land, sea, and rivers. Petroleum hydrocarbons are the most common environmental pollutants, and oil spills pose a great hazard to mangroves forests. This research was focused on the isolation and characterization of crude oil‐degrading bacteria from mangrove ecosystems at the Persian Gulf. Sixty‐one crude oil‐degrading bacteria were isolated from mangrove samples (plant, sediment, and seawater) that enriched in ONR7a medium with crude oil as only carbon source. Some screening tests such as growth at high concentration of crude oil, bioemulsifier production, and surface hydrophobicity were done to select the most efficient strains for crude oil degradation. Molecular identification of strains was carried out by amplification of the 16S rRNA gene by PCR. The results of this study were indicated that the quantity of crude oil‐degrading bacteria was higher in the root of mangrove plants compare to other mangrove samples (sediment and seawater). Also, identification results confirmed that these isolated strains belong to Vibrio sp. strain NW4, Idiomarina sp. strain BW32, Kangiella sp. strain DP40, Marinobacter sp. strain DW44, Halomonas sp. strain BS53, and Vibrio sp. strain DS35. The application of bioremediation strategies with these bacteria can reduce crude oil pollution in this important marine environment.  相似文献   

Petrochemistry and tectonic setting of the Davarzan-Abbasabad Eocene Volcanic (DAEV) rocks,NE Iran     

Habibollah Ghasemi  Mehdi Rezaei-Kahkhaei 《Mineralogy and Petrology》2015,109(2):235-252

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A probabilistic approach for computing water retention of particulate systems from statistics of grain size and tessellated pore network     

Richard Wan  Mehdi Pouragha  Mahdad Eghbalian  Jérôme Duriez  Tai Wong 《国际地质力学数值与分析法杂志》2019,43(5):956-973

The paper offers an analytical determination of the hydraulic properties of an unsaturated soil with reference to its retention curve, which describes the relationship between the volumetric water content and capillarity through matric suction. The analysis combines a particulate approach focused on the physics at the pore scale, including microstructural aspects, with a probabilistic approach where the void space and grain size are considered as random variables. In the end, the soil water characteristic curve of an unsaturated granular medium along a drying path can be derived analytically based on the sole information of particle size distribution. The analysis hinges on the tessellation of a wet granular system into an assemblage of tetrahedral unit cells revealing a pore network upon which capillary physics are computed with respect to pore throat invasion by a non-wetting fluid with evolving pendular capillary bridges. The crux of the paper is to pass from particle size probability distribution to a matching void space distribution to eventually reveal key information such as void cell and solid volume statistics. Making reasonable statistically based assumptions to render calculations tractable, the water retention curve can be readily constructed. Model predictions compare quite favourably with experimental data available for actual soils, especially in the high saturation range. Having a sound scientific basis, the model can be made amenable to address a variety of soils with a wider range of particle sizes.  相似文献   

Zoning Iran based on earthquake precursor importance and introducing a main zone using a data-mining process     

Pooyan Ramezani Besheli  Mehdi Zare  Ramezan Ramezani Umali  Gholamreza Nakhaeezadeh 《Natural Hazards》2015,78(2):821-835

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Micromechanical approach to swelling behavior of capillary-porous media with coupled physics     

Mahdad Eghbalian  Mehdi Pouragha  Richard Wan 《国际地质力学数值与分析法杂志》2019,43(1):353-380

A detailed multiscale analysis is presented of the swelling phenomenon in unsaturated clay-rich materials in the linear regime through homogenization. Herein, the structural complexity of the material is formulated as a three-scale, triple porosity medium within which microstructural information is transmitted across the various scales, leading ultimately to an enriched stress-deformation relation at the macroscopic scale. As a side note, such derived relationship leads to a tensorial stress partitioning that is reminiscent of a Terzaghi-like effective stress measure. Otherwise, a major result that stands out from previous works is the explicit expression of swelling stress and capillary stress in terms of micromechanical interactions at the very fine scale down to the clay platelet level, along with capillary stress emerging due to interactions between fluid phases at the different scales, including surface tension, pore size, and morphology. More importantly, the swelling stress is correlated with the disjoining forces due to electrochemical effects of charged ions on clay minerals and van der Waals forces at the nanoscale. The resulting analytical expressions also elucidate the role of the various physics in the deformational behavior of clayey material. Finally, the capability of the proposed formulation in capturing salient behaviors of unsaturated expansive clays is illustrated through some numerical examples.  相似文献   

Introducing a new conceptual instantaneous unit hydrograph model based on a hydraulic approach     

Setareh Khaleghi  Mohammad Parvin Nia 《水文科学杂志》2013,58(13-14):1967-1975

ABSTRACT

A novel approach is introduced for simulation of instantaneous unit hydrographs (IUHs). The model consists of a series of linear reservoirs that are connected to each other, and is referred to as the inter-connected linear reservoir model (ICLRM). By assuming the flow between two reservoirs is a linear function of the difference between the water levels in the reservoirs, a system of first-order linear differential equations is obtained as the ICLRM governing equation. By solving the equations, the discharge from the last reservoir is considered as an IUH. A small-scale laboratory device was constructed for the simulation of IUHs using the model. By studying four hydrographs extracted from the literature, and simulating them using both the ICLRM and the Nash model, it is concluded that the ICLRM can predict these hydrographs more accurately than the Nash model. Due to the simplicity of the construction and operation of the ICLRM and, more importantly, its visual aspect, the ICLRM may be considered as an effective educational tool for studying IUHs.  相似文献