牛肺肝素寡糖的制备     

于广利  王群  管华诗  徐家敏 《中国海洋大学学报(自然科学版)》2002,32(2):231-235

利用肝素酶 (Heparinase I,EC4 .2 .2 .7)对牛肺肝素进行控制酶解 ,混合寡糖经超滤、凝胶渗透色谱和高压液相色谱技术分离制备后 ,得到聚合度为 2 ,4 ,6 ,8,10 ,12 ,14和 2 0的寡糖纯品。各寡糖纯度采用毛细管电泳和聚丙烯酰胺凝胶电泳进行检验 ,寡糖结构采用核磁共振氢谱证实。  相似文献   

Fluorometric Determination of Chlorophyll   总被引：32，自引：0，他引：32  

Holm-Hansen  Osmund; Lorenzen  Carl J.; Holmes  Robert W.; Strickland  John D. H. 《ICES Journal of Marine Science》1965,30(1):3-15

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Nutrient attenuation in a shallow,gravel-bed river. II. Spatial and temporal changes in nitrogen dynamics and community metabolism     

J. Christopher Rutherford  Roger G. Young  John M. Quinn  Robert J. Wilcock 《新西兰海洋与淡水研究杂志》2020,54(3):410-430

ABSTRACT

Instream processes alter the concentration and bioavailability of nutrients as they are transported downstream. By relating primary production and periphyton composition to changes in nutrient concentration in a gravel-bed river this study made inferences about recycling and attenuation. Where dissolved inorganic nitrogen (DIN) was abundant, concentrations decreased linearly with distance but by less than required to meet the nitrogen demand of primary production. Where DIN was barely measurable photosynthesis was reduced but only by 50%. We infer that recycling sustained primary production even when DIN concentrations were negligibly small. One implication is that DIN removal underestimates attenuation. Further experimental research on recycling and improved modelling is required to better quantify the length of streams adversely affected by nutrients.  相似文献   

Extreme value estimates for arbitrary bandwidth Gaussian processes using the analytic envelope     

Robert D. Pierce 《Ocean Engineering》1985,12(6):493-529

A procedure is presented for the estimation of extreme values of stationary Gaussian random processes with arbitrary bandwidths. This approach is based on the analytic envelope defined by the Hilbert Transform; this envelope is Rayleigh distributed regardless of bandwidth. For experimentally derived data that has been converted into digital form, the Hilbert Transform is approximated using algorithms implemented on a digital computer to produce samples of the envelope's time history. Next, the degree of correlation between these envelope samples is taken into account using a method developed from simulation studies of a series of synthetic Gaussian time histories with varying bandwidths. Once this correlation effect has been estimated, the standard methods of order statistics are applied to these samples using the Rayleigh probability density function. Examples of applying this procedure to experimentally derived data are presented.  相似文献   

Sonographs of submarine sediment failure caused by the 1980 earthquake off northern California     

Michael E. Field  Robert K. Hall 《Geo-Marine Letters》1982,2(3-4):135-141

In 1980, a large earthquake caused extensive sediment failure on the shallow continental shelf off the Klamath River in northern California. Side-scan sonography was used to complement detailed geophysical profiling in identifying specific features and resolving modes of failure. The features include a nearly flat failure terrace mantled with sand boils, collapse craters and sediment flows, and bounded on the seaward side by a meandering continuous toe ridge. Seaward of the terrace lies a compression zone delineated by small pressure ridges. Our findings indicate a temporal progression of failure from lique-faction of shallow subsurface sand to lateral spread of intact blocks to sediment collapse and flow.  相似文献   

A seasonal survey of the sterol composition of the cockle cerastoderma edule: A comparison of polluted and non-polluted environments     

James A Ballantine  Angela Lavis  Robert J Morris 《Marine environmental research》1983,9(2):111-122

The sterol composition of a cockle population from an unpolluted environment in South Wales was monitored by monthly sampling over a 12-month period. In spite of the likely variation in dietary sterols during this period, the component sterols of the cockles showed no significant seasonal variation, indicating that the cockle was able to exhibit a high degree of control over the composition of its functional sterols.Dramatic differences were observed in the sterol compositions of a nearby cockle population from a highly polluted environment, near a sewage outfall; only three out of the ten sterols present were common to the unpolluted cockles. Forty-five per cent of the sterols from the ‘polluted’ samples contained fully reduced systems and included three new alkylated C-29, C-30 and C-31 stanols. It is considered possible that these structural changes may affect the stereochemistry of the functional sterols and hence alter the permeability of the animal's membranes. This may reflect a biochemical response to the polluted environment.  相似文献   

Characterization of chromophoric dissolved organic matter (CDOM) in the Baltic Sea by excitation emission matrix fluorescence spectroscopy   总被引：11，自引：0，他引：11  

Piotr Kowalczuk  Joanna Sto&#x;-Egiert  William J. Cooper  Robert F. Whitehead  Michael J. Durako 《Marine Chemistry》2005,96(3-4):273-292

Chromophoric dissolved organic matter (CDOM) is the major light absorber in the Baltic Sea. In this study, excitation emission matrix (EEM) fluorescence spectra and UV–visible absorption spectra of CDOM are reported as a function of salinity. Samples from different locations and over different seasons were collected during four cruises in 2002 and 2003 in the Baltic Sea in both Pomeranian Bay and the Gulf of Gdansk. Absorption by CDOM decreased with increased distance from the riverine source and reached a relatively stable absorption background in the open sea. Regression analysis showed that fluorescence intensity was linearly related to absorption by CDOM at 375 nm and a_CDOM(375) absorption coefficients were inversely related to salinity. Analysis of CDOM-EEM spectra indicated that a change in composition of CDOM occurred along the salinity gradient in the Baltic Sea. Analysis of percent contribution of respective fluorophore groups to the total intensity of EEM spectra indicated that the fluorescence peaks associated with terrestrial humic components of the CDOM and total integrated fluorescence decreased with decreasing CDOM absorption. In contrast, the protein-like fraction of CDOM decreased to a lesser degree than the others. Analysis of the percent contribution of fluorescence peak intensities to the total fluorescence along the salinity gradient showed that the contribution of protein-like fluorophores increased from 2.6% to 5.1% in the high-salinity region of the transect. Fluorescence and absorption changes observed in the Baltic Sea were similar to those observed in similar transects that have been sampled elsewhere, e.g. in European estuaries, Gulf of Mexico, Mid-Atlantic Bight and the Cape Fear River plume in the South Atlantic Bight, although the changes in the Baltic Sea occurred over a much smaller salinity gradient.  相似文献   

Rocking buoy wave energy converter     

Robert E. Salomon 《Ocean Engineering》1989,16(3)

A device for converting ocean wave energy into electricity utilizing a rocking buoy and a hydrogen pressure cell is described. The moored buoy contains a pneumatic tube filled with a dense liquid which rocks back and forth as a result of the ocean wave activity. Hydrogen gas, maintained above the dense fluid is alternatively compressed and expanded by the moving fluid. The hydrogen gas communicates with a hydrogen pressure cell and the resulting pressure difference across the cell leads to an electrical voltage and under load, to electrical power.  相似文献   

Coping with disaster: Rehabilitating coastal livelihoods and communities   总被引：4，自引：0，他引：4  

Robert S. Pomeroy  Blake D. Ratner  Stephen J. Hall  Jate Pimoljinda  V. Vivekanandan 《Marine Policy》2006,30(6):786-793

This paper examines lessons from past approaches to natural disasters, as well as early lessons from the post-2004 Asian tsunami rehabilitation, to draw out general principles for rehabilitating livelihoods in poor coastal communities. We contend that avoiding the mistakes of the past requires: (1) a framework for understanding the diversity of coastal people's livelihood strategies and the sources of their vulnerability, (2) a process for designing interventions that build on this understanding in order to strengthen and revitalize coastal communities, including a means of assessing and selecting the most promising livelihood options, and (3) a focus on the longer-term challenge of building future resilience and sustainability in the communities by addressing the root causes of vulnerability.  相似文献   

Ecology of beach wrack in northern New England with special reference to Orchestia platensis     

Manaf I. Behbehani  Robert A. Croker   《Estuarine, Coastal and Shelf Science》1982,15(6)

The northern New England beach wrack community with special reference to the cosmopolitan amphipod crustacean, Orchestia platensis, was examined at estuarine and open coastal habitats. Beach wrack was dominated by the plant genera Ascophyllum, Zostera, Spartina and Chondrus, and was most abundant during spring and late summer. Animal community numbers, biomass and frequency in fresh to moderately decomposed wrack were dominated by O. platensis throughout the year at all habitats; oligochaetes and Collembola were also important. The abundance of O. platensis showed high spatial and temporal variability, with low abundance generally associated with decreased amounts of wrack during colder months. An exception was the winter presence of the species at one estuarine habitat, in patchy aggregations within gravel-cobble refuges. The abundance of O. platensis averaged 1280 (0.04 m²)⁻¹, with a maximum of 7040 (0.04 m²)⁻¹. The life cycle of O. platensis is bivoltine, with summer-hatched young reaching maturity within 1 month. Laboratory studies indicate females with up to 4 broods (30 days)⁻¹, averaging 18 eggs brood⁻¹.Orchestia platensis is omnivorous, eating fresh plant tissue, live oligochaetes, Limulus eggs and diatom ‘fuzz’. The rate of laboratory consumption of algae and Zostera was 0.05 mg plant mg⁻¹ wet body weight day⁻¹. Presumptive predators of O. platensis are juvenile green crab, Carcinus maenus, and the earwig. Anisolabis maritima. The mobility, aggregation and aggressiveness of O. platensis assist the species in establishing and maintaining populations in the rigorous wrack habitat. The general competitive superiority of O. platensis over its congener, O. gammarella, and the co-occurrence of these species on both eastern and western Atlantic shores is discussed.  相似文献