Assessing neuroprotective P-glycoprotein activity at the blood-brain barrier in killifish (Fundulus heteroclitus) using behavioural profiles     

Bard SM  Gadbois S 《Marine environmental research》2007,64(5):679-682

The neuroprotective role of P-glycoprotein, a multixenobiotic resistance transporter (ABCB1/MDR1), in the blood-brain barrier in fish was examined using behavioural toxicological assays. P-glycoprotein acts as cellular efflux pump to prevent substrates from accumulating in the brain, including environmental contaminants such as ivermectin, a common aquaculture pesticide and mammalian anti-parasitic drug. The behavioural toxicological assays were developed to determine the neuropathological effect of ivermectin in killifish (Fundulus heteroclitus). P-glycoprotein function and thus blood-brain barrier integrity can be compromised by chemosensitizers that inhibit transport activity. Fish treated with ivermectin and the P-glycoprotein inhibitor cyclosporin A were significantly more sensitive and succumbed more rapidly to tilting, lethargy, slowing of pectoral-fin movement and loss of haptic-reactivity compared to fish treated with ivermectin-only. P-glycoprotein inhibition is associated with significantly earlier onset and increased mortality in ivermectin-exposed fish. Our results suggest that P-glycoprotein confers resistance against ivermectin-induced behavioural neuropathology and mortality in fish. This assay provides us with a non-invasive tool to study P-glycoprotein function in the blood-brain barrier and evaluate the behavioural effects of potential environmental neurotoxins.  相似文献   

Estimating a Lagrangian Length Scale Using Measurements of CO2 in a Plant Canopy     

Shannon E. Brown  Jon S. Warland  Eduardo A. Santos  Claudia Wagner-Riddle  Ralf Staebler  Meaghan Wilton 《Boundary-Layer Meteorology》2013,147(1):83-102

Analytical Lagrangian equations capable of predicting concentration profiles from known source distributions offer the opportunity to calculate source/sink distributions through inverted forms of these equations. Inverse analytical Lagrangian equations provide a practical means of estimating source profiles using concentration and turbulence measurements. Uncertainty concerning estimates of the essentially immeasurable Lagrangian length scale ( ${\mathcal{L}}$ ), a key input, impedes the operational practicality of this method. The present study evaluates ${\mathcal{L}}$ within a corn canopy by using field measurements to constrain an analytical Lagrangian equation. Measurements of net CO₂ flux, soil-to-atmosphere CO₂ flux, and in-canopy profiles of CO₂ concentration provided the information required to solve for ${\mathcal{L}}$ in a global optimization algorithm for 30-min time intervals. For days when the canopy was a strong CO₂ sink, the optimization frequently located ${\mathcal{L}}$ profiles that follow a convex shape. A constrained optimization then fit the profile shape to a smooth sigmoidal equation. Inputting the optimized ${\mathcal{L}}$ profiles in the forward and inverse Lagrangian equations leads to strong correlations between measured and calculated concentrations and fluxes. Coefficients of the sigmoidal equation were specific to each 30-min period and did not scale with any measured variable. Plausible looking ${\mathcal{L}}$ profiles were associated with negative bulk Richardson number values. Once the canopy senesced, a simple eddy diffusivity profile sufficed to relate concentrations and sources in the analytical Lagrangian equations.  相似文献   

Sampling Strategies for Volatile Organic Compounds at Three Karst Springs in Tennessee     

Shannon D. Williams  William J. Wolfe  James J. Farmer 《Ground Water Monitoring & Remediation》2006,26(1):53-62

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Surficial properties in Melas Chasma, Mars, from Mars Odyssey THEMIS data     

Shannon M Pelkey  Bruce M Jakosky  Philip R Christensen 《Icarus》2003,165(1):68-89

We examine the nature of the surface layer in a small area of the Melas Chasma region as determined from high-resolution thermal and visible Mars Odyssey Thermal Emission Imaging System (THEMIS) data as well as how our conclusions compare to past analyses. At THEMIS resolution, the thermal structure is dominated by local control and all significant thermal variations can be linked to morphology. Thus, THEMIS provides us with detailed images that contain thermophysical information as well, allowing us to create a surficial geologic map intended to reflect the surface structure of the region. Eight units have been defined: (i) blanketed plateaus with thermally distinct craters and fractures, (ii) blanketed canyon walls with rocky edges, (iii) indurated and/or rocky canyon wall slide material partially covered by aeolian material, (iv) an anomalous wall region with fluvial-like depressions partially filled with particulate material, (v) indurated and/or rocky ridged and non-ridged canyon floor landslide material mingled with aeolian material, (vi) sand sheets, (vii) indurated and/or rocky rounded blocks intermingled with small areas of aeolian material, and (viii) transverse dunes. The THEMIS thermal data support conclusions from previous studies but also reveal much more structure than was seen in the past. We have found that all significant thermal variations in this region can be linked to morphology but all morphological variations cannot be linked to significant thermal variations. THEMIS visible images provide an intermediate resolution that bridges the gap between MOC and Viking and allow for a more meaningful interpretation of the geologic context of a region. Surfaces indicate that landslides were an important geologic process long ago, shaping the canyon walls and floor, while aeolian processes have subsequently altered the surface layer in many locations and may still be active.  相似文献   

Anatomy of a closing window: Vulnerability to changing seasonality in Interior Alaska   总被引：1，自引：0，他引：1  

Shannon M. McNeeley  Martha D. Shulski 《Global Environmental Change》2011,21(2):464-473

Alaska is among the fastest warming places on Earth, and the Interior region is warming the most statewide. Significant regional-scale ecosystem services disruptions are affecting Alaska Natives’ subsistence hunting and harvest success. The well-being of rural native communities is still highly dependent on access and ability to harvest wild foods such as salmon and moose (Alces alces gigas) among many others. Over the last decade communities in the Koyukuk-Middle Yukon (KMY) region of Interior Alaska report an inability to satisfy their needs for harvesting moose before the hunting season closes, citing warmer falls, changing precipitation and water levels, and the regulatory framework as primary causes. Through the integration of ethnographic methods to record indigenous observations and understanding of climate (IC) with analysis of meteorological data, we provide a comprehensive picture of vulnerability to recent warming trends in the Koyukuk-Middle Yukon region of Interior Alaska, one that captures more than statistical analysis of “norms” can provide. We will demonstrate how low exposure resulting in a small shift in seasonality has truly socially significant effects to people “on the ground” when community sensitivity is high because of the convergence of multiple social-ecological stressors. In this case, a seemingly small climatic exposure when combined with high social-ecological system sensitivity results in vulnerability to this climate change-related seasonality shift because of: (a) the effects on moose and the social-ecological dynamics of the system, and (b) the importance of this time of the year to meeting annual subsistence needs.  相似文献   

Quaternary eolian dunes in the Savannah River valley,Jasper County,South Carolina,USA     

Christopher S. Swezey  Arthur P. Schultz  Wilma Alemán González  Christopher E. Bernhardt  William R. Doar III  Christopher P. Garrity  Shannon A. Mahan  John P. McGeehin 《Quaternary Research》2013

Sand hills in the Savannah River valley in Jasper County (South Carolina, USA) are interpreted as the remnants of parabolic eolian dunes composed of sand derived from the Savannah River and stabilized by vegetation under prevailing climate conditions. Optically stimulated luminescence ages reveal that most of the dunes were active ca. 40 to 19 ka ago, coincident with the last glacial maximum (LGM) through early deglaciation. Modern surface winds are not sufficient for sustained eolian sand transport. When the dunes were active, winds blew at velocities of at least 4 m/s from west to east, and some vegetation was present. The ratio of annual precipitation to potential evapotranspiration (P:PE) was less than the modern ratio of 1.23 and may have been < 0.30, caused by stronger winds (which would have resulted in greater evaporation) and/or reduced precipitation. The Savannah River dunes are part of a larger assemblage of eolian dunes that were active in the eastern United States during and immediately after the LGM, suggesting that eolian sediment behavior in this region has been controlled by regional forcing mechanisms during the Quaternary.  相似文献   

Desert Pavement Disturbance and Artifact Taphonomy: A Case Study from the Eastern Libyan Plateau,Egypt     

Katherine A. Adelsberger  Jennifer R. Smith  Shannon P. McPherron  Harold L. Dibble  Deborah I. Olszewski  Utsav A. Schurmans  Laurent Chiotti 《Geoarchaeology》2013,28(2):112-130

Accretionary desert pavements on the eastern Libyan Plateau of central Egypt support a rich Middle and Upper Paleolithic artifact assemblage exhibiting intensive blank production and minimal tool production. These assemblages appear to be in primary context with numerous examples of lithic refits showing on‐site lithic production. However, the smallest (length ≤2.5 cm) archaeological fragments are recovered at a much lower rate on this desert pavement surface than expected given comparable data from lithic assemblages in cave and shelter contexts in France. Excavation of archaeological contexts on the Libyan Plateau reveals the loss of small artifact fragments into the subsurface due to aeolian accumulation of silts, whereas geomorphic examination of desert pavement surfaces suggests a potential for relatively isolated bioturbation as a source of lateral and vertical disturbance of desert pavement surfaces over small areas. Archaeologists should be aware of the potential for long‐term assemblage stability as well as small artifact burial in surficial desert pavement contexts.  相似文献   

Measuring Change in Place Values for Environmental and Natural Resource Planning Using Public Participation GIS (PPGIS): Results and Challenges for Longitudinal Research   总被引：2，自引：0，他引：2  

Greg Brown  Shannon Donovan 《社会与自然资源》2014,27(1):36-54

Landscape values are a type of place value and are identified and mapped using public participation GIS (PPGIS). PPGIS engages nonexperts to identify important spatial information for environmental or natural resource planning. In 1998, we used PPGIS to identify landscape values for the Chugach National Forest (Alaska) plan revision process. In 2012, we conducted a longitudinal study of the same national forest using Internet PPGIS to identify changes in landscape values. The empirical results indicate stability in landscape values both in importance and spatial distribution. However, the use of different PPGIS methods (paper map vs. Internet) in the longitudinal study also introduced challenges in interpreting and explaining the spatial results. We discuss trade-offs in conducting longitudinal PPGIS research using mixed methods. PPGIS appears well suited for public lands planning, and national forest planning in particular, but barriers to use, such as regulatory approval, remain formidable.  相似文献   

Reductive dissolution of goethite and the subsequent transformation of 4-cyanonitrobenzene: Role of ascorbic acid and pH     

Jean M. Smolen  Megan A. McLaughlin  Michael J. McNevin  Angela Haberle  Shannon Swantek 《Aquatic Sciences - Research Across Boundaries》2003,65(3):308-315

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VARNET-96: three-dimensional upper crustal velocity structure of SW Ireland   总被引：2，自引：0，他引：2  

M. Landes  B. M. O'Reilly  P. W. Readman  P. M. Shannon  C. Prodehl 《Geophysical Journal International》2003,153(2):424-442

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