CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES     

郭华荣  张士璀  李红岩  童裳亮  相建海 《中国海洋湖沼学报》2002,20(3):226-231

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.  相似文献   

山东温泉旅游资源分析及科学发展思考   总被引：1，自引：0，他引：1  

李宇荣  王艳平 《山东国土资源》2008,24(3):35-38

山东省温泉资源分布广泛、储量大，利用历史十分悠久，在温泉资源开发应用过程中，应根据其分布、类型、开发利用现状，并结合国内外的科技成果而进行科学开发与综合利用，建设既有地方民俗文化特色又与国际接轨的可持续发展模式。  相似文献   

矢量数据栅格化的一种有效方法——环绕数法   总被引：4，自引：0，他引：4  

武广臣  左建章  刘艳  李丽 《测绘科学》2009,34(1)

矢量数据栅格化是GIS数据处理的一项重要任务。目前,已存在多种栅格化方法,但是每种方法都有各自的局限性。本文首次提出了环绕数法,它基于计算几何转角理论,通过计算跨越数来确定环绕数,是一种简单、高效、易于实现的栅格化方法。应用环绕数法,不但可以完成简单多边形的栅格化,而且可以完成自交多边形的栅格化。  相似文献   

土地征用制度改革的探索   总被引：1，自引：0，他引：1       下载免费PDF全文

秦泗彦  田宏红  张传亮 《山东国土资源》2003,19(6):29-31,41

征地补偿安置是当前群众反映最强烈并迫切要求解决的问题。要解决该问题必须进行土地征用制度改革．由政府按照市场公平原则向农民集体征购土地，实行统一的土地征购制度。  相似文献   

Technique for Controlling Spread of Limnotic Oncomelania   总被引：1，自引：0，他引：1  

李大美  王祥三  LAI Yonggen 《中国海洋湖沼学报》2003,21(3):251-260

Schistosomiasis is a parasitic disease mostly found in areas along the Changjiang River of China. The disease is spread solely through an intermediary named oncomelania, so its spread of schistosomiasis can be controlled by properly designing water intakes which prevent oncomelania from entering farming land or residential areas. This paper reports a successful design process and a new oncomelania-free intake device. The design of the new intake is based on a sound research program in which extensive experimental studies were carried out to gain knowledge of oncomelania eco-hydraulic behavior and detailed flow field information through CFD simulation.  相似文献   

未来的国际天球参考架     

金文敬  李东明  唐正宏  王叔和 《紫金山天文台台刊》2003,(1)

本文简述了国际天球参考架的发展历史和现在射电参考架的现状—基准源选择的标准和参考架的稳定性。描述了地面上光学观测在依巴谷参考架的维持和加密的一系列工作。介绍由天体测量卫星GAIA和SIM给出的天球参考架可能逵到的精度。详述了在今后十年中地面天体测量的作用以及正在开展有关天球参考架的研究课题 ,同时也列出了我国正在和即将开展天体测量的几个研究课题  相似文献   

Report on Pontoniinae shrimps （Crustacea：Decapoda）collected by Joint Chinese—German Marine Biology Expeditions to Hainan lsland ,South China Sea：Ⅱ.Harpiliopsis,Ischnopontonia,Jocaste,Palae—mone     

李新正  刘瑞玉 《中国海洋湖沼学报》2003,21(2):154-165

The present paper reports 9 species of pontoniine shrimps collected from Hainan IS-land ,South China Sea ,by the Joint Chinese-German Marine Biology Expeditions,in which Peri-climenaeus arabicus (Calman,1939) and Periclimenaeus hecate (Nobili,1904) are recorded for the first time from Hainan Island.  相似文献   

地倾斜台站数字化、模拟观测数据频率特性研究   总被引：2，自引：2，他引：0  

李正媛  陈鹏  林穗平  陈志遥 《大地测量与地球动力学》2003,23(1):74-77

分析了四川、云南的部分地倾斜数字化观测与模拟观测资料的特性，从频率范围，周期10天以上的FFT谱特性，固体潮波振幅，相位特征值等方面，论述了地倾斜数字化资料较模拟记录资料的信息组成变化，并对资料的分析与应用进行了初步探讨。  相似文献   

CORROSION AND ADHERENCE AT SEA OIL-PLATFORM   总被引：1，自引：0，他引：1  

马士德  李言涛  黄修明  郑国安  常伟  吴培辉 《中国海洋湖沼学报》1995,13(3):253-257

Measurements of the adhesive corrosion and protective potentials of 3 Bohai Sea oilplatforms under different cathodic protection showed corrosion still occurred on these platforms undertheir designed protective potentials. Preliminary conclusions from indoor experiments on the relation betweenadhesive corrosion and cathodic protection showed adhering organisms caused the corrosion onthese platforms.  相似文献   

沉积物粒度分析在厦门市第四纪环境研究和地层划分对比中的应用   总被引：6，自引：0，他引：6  

张璞  陈建强  田明中  朱金芳  黄宗林  江春亮 《地球科学与环境学报》2005,27(1):88-94

采用MS2000型激光粒度分析仪进行测试，利用计算机粒度分析软件对数据进行整理和计算，绘制出样品的频率曲线、概率累积曲线以及粒度众数位值曲线等图件并进行沉积环境分析。厦门地区第四纪环境演化经历了中更新世同安组(含泥中粗砂、含泥细中砂、砂为主)海积一冲积一更新统上部龙海组(含泥粗中砂、含泥细中砂、含泥粗砂、粗砂、细中砂、含细砾中粗砂、中细砂等)冲积一洪冲积一更新世上部东山组(砂、砂砾、含泥中粗砂、粗砂、泥质中细砂、细砂、中粗砂等)冲洪积及部分海积(粉砂、细粉砂、含碳质泥、粘土、淤泥质粘土、粉砂质粘土等)一全新世长乐组(粉砂，淤泥质粘土、粉砂质粘土、碳质粘土、含砂淤泥质粘土，粉砂质亚粘土、粉细砂亚粘土等)海湾沉积。在解释环境变化的同时，说明粒度变化曲线在一定程度上可以作为地层划分的依据之一，并以此对研究区地层进行了详细划分。  相似文献