Encystment and excystment of <Emphasis Type="Italic">Gyrodinium instriatum</Emphasis> Freudenthal et Lee     

Tomoyuki Shikata  Sou Nagasoe  Tadashi Matsubara  Yasuhiro Yamasaki  Yohei Shimasaki  Yuji Oshima  Takuji Uchida  Ian R. Jenkinson  Tsuneo Honjo 《Journal of Oceanography》2008,64(3):355-365

In the present study, we have investigated the conditions influencing encystment and excystment in the dinoflagellate Gyrodinium instriatum under laboratory conditions. We incubated G. instriatum in modified whole SWM-3 culture medium and in versions of modified SWM-3 from which NO₃ ⁻, PO₄ ³⁻, NO₃ ⁻ + PO₄ ³⁻, or Si had been omitted and observed encystment. Percentage encystment was high in the media without N and without P, while the percentage encystment in the medium lacking both N and P was highest. Moreover, to investigate N or P concentration which induced the encystment, Gyrodinium instriatum was also incubated in media with different concentrations of inorganic N and P; the concentrations of NO₂ ⁻ + NO₃ ⁻ and PO₄ ³⁻ were measured over time. The precursors of cysts appeared within 2 or 3 days of a decrease in NO₂ ⁻ + NO₃ ⁻ or PO₄ ³⁻ concentration to values lower than 1 μM or 0.2 μM, respectively. When cysts produced in the laboratory were incubated, we observed excystment after 8–37 days, without a mandatory period of darkness or low temperature. We incubated cysts collected from nature at different temperatures or in the dark or light and observed excystments. Natural cysts excysted at temperatures from 10 to 30°C, in both light and dark, but excystment was delayed at low temperatures. These studies indicate that G. instriatum encysts in low N or P concentration and excysts over a wide temperature range, regardless of light conditions, after short dormancy periods.  相似文献   

Upwelling of oxygen-depleted water (Sumishio) in Omura Bay,Japan     

Tetsuya Takahashi  Hideaki Nakata  Keiji Hirano  Kazumi Matsuoka  Mitsunori Iwataki  Hitoshi Yamaguchi  Tomoyuki Kasuya 《Journal of Oceanography》2009,65(1):113-120

Wind-induced circulation and the distribution of hypoxia corresponding to the upwelling of oxygen-depleted water (called “Aoshio” in Japan and “Sumishio” locally in Omura Bay) in Omura Bay, Japan, was examined field observations and by three-dimensional modeling. During the calm weather in summer, well-mixed strait water, in rich oxygen at the mouth of the bay intruded into the middle layer of the bay, southward and northward along the west and east coast, respectively, forming basin-scale cyclonic circulation. A stagnant water mass was formed below the center of this cyclonic circulation, and it became hypoxic water. As a result of the prevailing strong southeast (northeast) wind, the bottom hypoxia moved in a southeasterly (northeasterly) direction. This induces the upwelling of hypoxic water, accompanied by mass mortality of marine organisms.  相似文献   

Constraining paleo-latitude of a biogeographic boundary in mid-Panthalassa: Fusuline province shift on the Late Guadalupian (Permian) migrating seamount     

Akihisa Kasuya  Yukio Isozaki  Hisayoshi Igo 《Gondwana Research》2012,21(2-3):611-623

The Guadalupian paleo-atoll limestone (Iwato Formation) in SW Japan was primarily formed in low-latitude mid-Panthalassa and was later tectonically accreted to South China (Japan) margin during the Jurassic. The present biostratigraphic study clarified that the Iwato Formation consists of 5 biostratigraphical intervals; i.e. four fusuline assemblage zones (Assemblage zones 1 to 4) and a barren interval on the top. Assemblage zones 1 to 4 correspond to the Neoschwagerina craticulifera Zone, N. margaritae Zone, Yabeina globosa Zone, and Lepidolina multiseptata Zone of the conventional Tethyan fusuline stratigraphy, respectively. The present study newly clarified the following significant aspects of paleobiogeography of the Permian fusulines as to the extinction of large-tested taxa in the latest Guadalupian. 1) The long unknown stratigraphic relationship was documented for the first time between the Yabeina-dominant interval and the overlying Lepidolina-dominant one within a single limestone unit. 2) The occurrence of Lepidolina cf. kumaensis Kanmera, the unique last runner of large-tested fusuine, was detected for the first time in mid-oceanic paleo-atoll limestones. 3) With respect to the northbound migration history of the paleo-seamount capped by the Iwato Formation, the development of the two coeval fusuline biogeographic territories in the low-latitude Panthalassa, i.e., the Yabeina territory on the south and the Lepidolina territory on the north, was confirmed. 4) The paleo-latitude of the biogeographic boundary between the Yabeina and Lepidolina territories is constrained around 12° in the southern hemisphere on the basis of the latest geomagnetic data from the same limestone. This new approach utilizing biostratigraphy on ancient migrating seamounts coupled with geomagnetic paleo-latitude data is applicable to other cases in different time-space co-ordinates and of other fossil groups for constraining position of ancient biogeographic boundaries within lost oceanic domains of deep past.  相似文献   

Large-scale landslides in Toyama Prefecture,central Japan,and their probable relationship with earthquakes     

Satoru Kojima  Tamotsu Nozaki  Hidehisa Nagata  Ryota Tanahashi  Ryoich Kondo  Noriaki Okamura  Kazuhiro Suzuki  Akiko Ikeda  Toshio Nakamura  Tomoyuki Ohtani 《Environmental Earth Sciences》2014,71(6):2753-2763

Large-scale landslides along the Kubusu and Besso rivers in Toyama Prefecture are developed in the Miocene Iwaine Formation, which is composed of andesitic lava, tuff, and tuff breccia. In the middle member of this formation, the tuff is easily altered to montmorillonite-bearing rock, and subsequently plays an important role in the development of landslides events, which tend to be large-scale events, as the massive lava of the upper member forms a cap rock over the tuff. The Kiritani and Koinami basins, which are flat intermontane basins located along the Kubusu and Besso rivers, respectively, are interpreted as landslide-dammed lakes, later filled with sediment. Accelerator mass spectrometry ¹⁴C ages show that the landslides forming each dam occurred simultaneously, at approximately 2500 BP. These ages were measured from wood fragments embedded in the landslide material of Kiritani, and from an in situ stump drowned during the impoundment of Koinami. If the trigger of these landslides was an earthquake, it is most likely to have been the penultimate event along the Atotsugawa fault zone.  相似文献   

Generation of tidal bedding in a circular flume experiment: formation process and preservation potential of mud drapes     

Tomoyuki Sato  Keisuke Taniguchi  Tomohiro Takagawa  Fujio Masuda 《Geo-Marine Letters》2011,31(2):101-108

Flume experiments aimed to produce flaser bedding were conducted using fine sand and clay in a circular flume. The formation process of mud drapes during the slack-water stage was revealed in detail. When the tidal current declines, a uniform mobile mud layer initially settles from suspension and drapes the entire rippled sand bed (type A mud). When the remaining flow velocity is very low, a more fluid mud begins to settle out (type B mud) that preferentially fills the ripple troughs, the ripples and mud together forming a flat surface. At slack tide, the two-phase mud drape is temporarily stationary. After the onset of the reversed flow phase, most of the type B mud is resuspended, while the type A mud is eroded from the crests, leaving behind a remnant mud drape (flaser) in the troughs that is subsequently buried by migrating ripples. Type B mud generally contains variable amounts of sand derived from eroded ripple crests, but does not show any visible internal sedimentary structures. Type A mud represents the ‘mud drapes’ commonly described in the literature, the temporary existence of type B mud having gone unnoticed because of its low preservation potential. When present, it can be recognized by its sand content and the occurrence of flame structures in ripple troughs. Tidal deposits reflecting the existence and depositional characteristics of both type A and type B mud are found in, for example, the macrotidal Oligocene Ashiya Group, Japan.  相似文献   

Cell contact-dependent lethal effect of the dinoflagellate Heterocapsa circularisquama on phytoplankton–phytoplankton interactions     

Yasuhiro Yamasaki  Yanan Zou  Jyoji Go  Tomoyuki Shikata  Yukihiko Matsuyama  Kiyohito Nagai  Yohei Shimasaki  Kenichi Yamaguchi  Yuji Oshima  Tatsuya Oda  Tsuneo Honjo 《Journal of Sea Research》2011,65(1):76-83

We used bi-algal culture experiments to investigate and verify the roles of growth interaction between Heterocapsa circularisquama and Prorocentrum dentatum in monospecific bloom formation. Growth of H. circularisquama was slightly inhibited when inoculated at 10² cells mL^–1 along with P. dentatum at 10⁴ cells mL^–1. In other combinations of inoculation densities, P. dentatum density rapidly decreased to extremely low levels in the presence of H. circularisquama. We used a mathematical model to simulate growth and interactions of H. circularisquama and P. dentatum in bi-algal cultures. The model indicates that one species will always inhibit the growth of the other and that the relative initial cell densities of the species are critical in determining the outcome. When cultured together under conditions without cell contact, growth of H. circularisquama and P. dentatum was not inhibited. As with P. dentatum, the growth of Heterosigma akashiwo and Skeletonema costatum was inhibited in intact cell suspensions with H. circularisquama, but a nontoxic species, Heterocapsa triquetra, did not affect the growth of P. dentatum or the other species. Similarly, cell suspensions of H. circularisquama showed hemolytic activity toward rabbit erythrocytes, but those of H. triquetra did not. In addition, the cell-free supernatant of H. circularisquama cultures showed no significant hemolytic activity. These results suggest that H. circularisquama causes lethality in P. dentatum by direct cell contact in which live-cell-mediated hemolytic activity might be a contributing factor.  相似文献   

Brownish discoloration of the summit crater lake of Mt. Shinmoe-dake,Kirishima Volcano,Japan: volcanic–microbial coupled origin     

Shinji Ohsawa  Kenji Sugimori  Hiroshi Yamauchi  Tomoyuki Koeda  Hiroaki Inaba  Yoshihisa Kataoka  Tsuneomi Kagiyama 《Bulletin of Volcanology》2014,76(5):1-10

A drastic change in lake water color from blue-green to brown was observed in the summit crater lake of Mt. Shinmoe-dake, Kirishima Volcano about 8 months after its 2008 eruption. The color change lasted for about 2 months (April–June 2009). The discoloration was attributed to a brownish color suspension that had formed in the lake water. X-ray fluorescence and Fourier transform infrared analyses of a sample of the suspension identified schwertmannite (Fe₈O₈(OH)₆(SO₄)). A cultivation test of iron-oxidizing bacteria for the sampled lake water with lakebed sediment revealed that the crater lake hosts iron-oxidizing bacteria, which likely participated in schwertmannite formation. We suggest that pyrite (FeS₂) provided an energy source for the iron-oxidizing bacteria since the mineral was identified in hydrothermally altered tephra ejected by the August 2008 eruption. From consideration of these and other factors, the brownish discoloration of the summit crater lake of Mt. Shinmoe-dake was inferred to have resulted from a combined volcanic–microbial process.  相似文献   

Experimental Study of Sulfur Solubility in Silicate Melts Coexisting with Graphite as a Function of Silicate Melt Composition     

Tomoyuki Tsujimura  Arashi Kitakaze 《Resource Geology》2005,55(1):55-60

Abstract. Sulfur (S) solubility is determined in silicate melts, in particular with low-FeO content coexisting with graphite. In a high FeO range (>10 mol %), the S solubility is strongly dependent on the FeO content in silicate melt, which is in good agreement with previous works. In an intermediate FeO range (1∼10 mol %), the S solubility in melts with a multi-component system is higher than that in melts with a simple system. The SKα shift of the electron microprobe shows the dissolve sulfur is all sulfide. A positive dependence is observed between CaO and S content in the melt undoped with FeO, but the dissolved sulfur is sulfate. The relationship between melt composition, S content, and S oxidation state is discussed.  相似文献   

Electrical conductivity measurement of gneiss under mid- to lower crustal P–T conditions     

Kiyoshi Fuji-ta  Tomoo Katsura  Takuya Matsuzaki  Masahiro Ichiki  Tomoyuki Kobayashi 《Tectonophysics》2007,434(1-4):93-101

We conducted electrical conductivity measurements perpendicular and parallel to mineral foliation in dry gneiss at up to 1000 K and a constant pressure of 1 GPa. The analyzed gneisses were obtained from the Higo metamorphic belt, Kyushu, Japan. As the metamorphic conditions of these rocks have been well determined by previous studies, we were able to select samples that were representative of the middle to lower crust. Prior to the conductivity measurements, the samples were maintained at the maximum temperature for a long period, until the electrical conductivity had stabilized. Our experiment results reveal linear and reproducible conductivity data between temperatures of 600 and 1000 K. Conductivity measured perpendicular and parallel to foliation differ by an order of magnitude over the same temperature window. A plausible explanation for this discrepancy in conductivity is the contrasting configuration of minerals in the two sample orientations, as observed by backscattered electron image (BEI) and electron probe microanalysis (EPMA). We evaluated the conductivity and computed activation energy for each of the samples and compared the results with those of previous studies; our results are consistent with the conductivity values reported for other types of rocks. We also compared the experiment results with data derived from electromagnetic (EM) soundings. Electrical conductivity measurements undertaken perpendicular to foliation can account for the subsurface conductivity structure beneath central Kyushu, Japan.  相似文献   

Detailed Observation of Spatial Abundance of Clam Larva Ruditapes philippinarum in Tokyo Bay, Central Japan     

Tomoyuki Kasuya  Masami Hamaguchi  Keita Furukawa 《Journal of Oceanography》2004,60(3):631-636

The spatial distribution of the larval abundance of the clam Ruditapes philippinarum has been investigated at 65 stations throughout Tokyo Bay on August 2, 2001. The large number of small D-shaped larvae that were found shortly after hatching in the waters around the Banzu, Futtu, and Sanmaizu-Haneda areas indicates that spawning populations in these areas probably contribute greatly to the larval supply in the bay. Small larvae also occurred abundantly around the Yokohama and Ichihara port areas, suggesting that these port regions play a role in the transport of larvae into Tokyo Bay. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献