鱼类淋巴囊肿病毒靶器官的组织病理研究   总被引：9，自引：3，他引：9  

绳秀珍  战文斌 《中国海洋大学学报(自然科学版)》2006,36(5):749-753

探讨病毒传播途径,运用组织病理学技术,对患淋巴囊肿病毒病的牙鲆和鲈鱼的组织进行显微观察,并对病毒感染的靶器官进行了研究。结果表明,在牙鲆体表皮下结缔组织中,当大量成纤维细胞膨大、变圆,大小为10~18μm时,细胞膜外未出现囊壁,少数细胞的细胞质中观察到嗜碱性物质;18~20μm的膨大细胞在细胞膜外出现囊壁,细胞质内出现嗜碱性物质;>20μm的细胞具有了淋巴囊肿细胞的典型特征。另外,在牙鲆鳃、肠上皮下结缔组织和粘膜下层中,脾、头肾边缘组织和表面系膜、肝表面系膜,以及鲈鱼的鳃等部位均观察到淋巴囊肿细胞,并在牙鲆和鲈鱼脾内观察到膨大细胞。因此,淋巴囊肿病毒的靶器官主要是鱼体表皮下结缔组织,其次是鳃、体腔膜、肠、脾和头肾。鱼体表皮下结缔组织是淋巴囊肿病毒病的检测、检疫和早期诊断的最佳样品。  相似文献   

大通湖中华绒螯蟹(Eriocheir sinensis)自然生产潜力估算模型探讨     

杨品红  邹万生  刘良国  徐黎明  王文彬  陈红文  唐琳  曹苗  黄春红  罗玉双  韩庆 《海洋与湖沼》2015,46(4):833-837

人放天养的中华绒螯蟹(Eriocheir sinensis)质量好、个体大、耐运输、肉质鲜美,是优质高档蟹的代表,但产量严重不足。建立一套湖泊中华绒螯蟹自然生产潜力估算的模型来估算湖泊中华绒螯蟹自然生产潜力,确定放养量,以达到湖泊中华绒螯蟹稳产已迫在眉睫。本文根据近20年来作者对湖泊中华绒螯蟹放养的经验,结合灰估理论,提出蟹自然生产潜力:W=[B1K1C1/R1+B2K2C2/R2]·(B1+B2)2/B12+B2和蟹种放养量:W0=P0[B1K1C1/R1+B2K2C2/R2]·U/P估算模型,并与生产实践进行求证。其结果与生产实践存在较好的一致性。其估算结果可作为指导湖泊中华绒螯蟹生产的依据。  相似文献   

紫锥菊提取物对大菱鲆(Scophthalmus maximus)的非特异性免疫功能的影响     

秦志华  董文宾  姜令绪  王仁杰  张启迪  单虎  雷霁霖 《海洋与湖沼》2015,46(3):665-669

本文旨在探讨肌肉注射不同浓度的紫锥菊提取物对大菱鲆(Scophthalmus maximus)的非特异性免疫功能的影响,试验选取体重(250±20)g的大菱鲆作为试验动物,分别注射10、20、40mg/m L浓度的紫锥菊提取物,试验共进行28d,每隔7d采集血液测试非特异性免疫指标,第28天用迟缓爱德华氏菌(Edwardsiella tarda)攻毒,连续观察7d,测定其免疫保护率。结果显示肌肉注射紫锥菊提取物对大菱鲆超氧化物歧化酶(SOD)活力和溶菌酶活力(LSZ)的影响极显著(P0.01);对吞噬细胞吞噬率(PP)的影响显著(0.01P0.05),对吞噬细胞呼吸爆发活力的影响极显著(P0.01)。攻毒试验表明,紫锥菊提取物能够降低迟缓爱德华氏菌攻毒后鱼的死亡率,提高其免疫保护率,尤其是40mg/m L组。本试验研究表明,紫锥菊提取物能显著的提高大菱鲆的非特异性免疫功能,且以注射浓度40mg/m L的效果最好。  相似文献   

On the hydro-spectrum of deep wells and the response ability of well-aquifer system     

Wen-Bin Jian  Bao-Ren Chen  Shu-Yun Liu 《地震学报(英文版)》1995,8(4):639-645

onclusions 1) A set of period components corresponding well to those of Earth tides and barometric pressure can be separated from the hydro-spectra of deep well. The well-aquifer system responds to these components in some degree, which shows that groundwater regime is influenced by Earth tides and barometric pressure. However, in different frequency bands a well-aquifer system possesses different transfer ability for various inputs. 2) The transfer function quantitatively describes the response ability of well-aquifer system to Earth tides and barometric pressure. It is a characteristic parameter that the system itself possesses. According to the transfer function value of well-aquifer system to a certain input factor, we can objectively understand the influence of the input on the groundwater regime in a deep well. This is of important significance for revealing the formation of groundwater regime in a deep well and for evaluating the seismic supervising well. The work was supported by National Natural Science Foundation of China (No. 49372149).  相似文献   

The preliminary interpretation of deep seismic sounding in western Yunnan   总被引：2，自引：0，他引：2  

Zhong-Yang Lin  Hong-Xiang Hu  Wen-Bin Zhang  Hui-Fen Zhang  Zheng-Qin He  Zhen-Ming Lin  Tao-Xing Qiu 《地震学报(英文版)》1993,6(4):867-881

The preliminary interpretation of Project western Yunnan 86–87 is presented here. It shows that there obviously exists lateral velocity heterogeneity from south to north in western Yunnan. The depth of Moho increases from 38 km in the southern end of the profile to 58 km in its northern end. The mean crustal velocity is low in the south, and high in the north, about 6.17–6.45 km/s. The consolidated crust is a 3-layer structure respectively, the upper, middle and lower layer. P ₁ ⁰ is a weak interface the upper crust, P ₂ ⁰ and P ₃ ⁰ are the interfaces of middle-upper crust and middle-lower crust respectively. Another weak interface P ₃ ^0′ can be locally traced in the interior of the lower crust. Interface Pg is 0–6 km deep, interface P ₁ ⁰ 9.2–16.5 km deep, and interfaces P ₂ ⁰ and P ₃ ⁰ respectively 17.0–26.5 km, 25.0–38.0 km deep. The velocity of the upper crust gradually increases from the south to the north, and reaches its maxmium between Nangaozhai and Zhiti, where the velocity of basement plane reaches 6.25–6.35 km/s, then it becomes small northward. The velocity of the middle crust varies little, the middle crust is a low velocity layer with the velocity of 6.30 km/s from Jinhe-Erhai fault to the north. The lower crust is a strong gradient layer. There exists respectively a low velocity layer in the upper mantle between Jinggu and Jingyunqiao, and between Wuliangshan and Lancangjiang fault, the velocity of Pn is only 7.70–7.80 km/s, it is also low to the north of Honghe fault, about 7.80 km/s. Interface P_6/0 can be traced on the top of the upper mantle, its depth is 65 km in the southern end of the profile, and 85 km in the northern end. The Chinese version of this paper appeared in the Chinese edition ofActa Seismologica Sinica,15, 427–440, 1993.  相似文献   

Enhancement of seasonal prediction of East Asian summer rainfall related to western tropical Pacific convection     

Doo Young Lee  Joong-Bae Ahn  Jin-Ho Yoo 《Climate Dynamics》2015,45(3-4):1025-1042

  相似文献   

大菱鲆mIgD重链基因的克隆与表达分析   总被引：1，自引：0，他引：1  

陈孔茂  唐小千  绳秀珍  邢婧  战文斌 《中国海洋大学学报(自然科学版)》2014,44(3)

利用RACE技术克隆获得大菱鲆(Scophthalmus maximus)膜结合型免疫球蛋白D(Membrane-bounded Ig,mIgD)重链基因的cDNA序列全长,该基因全长3 357bp,包含1个2 997bp的开放阅读框,编码999个氨基酸,基因结构为VDJ-μ1-δ1-δ2-δ3-δ4-δ5-δ6-δ7-TM。重链恒定区δ1~δ7氨基酸序列同源比对结果显示,其与庸鲽(78%)、鳜(71%)、牙鲆(68%)具有较高的同源性;多序列比对结果显示,大菱鲆mIgD的每个δ恒定区内均存在色氨酸与半胱氨酸保守位点。利用邻位相连法构建硬骨鱼类免疫球蛋白系统发育树,结果显示,鱼类IgD聚为一大支,大菱鲆IgD与庸鲽及牙鲆聚为一支。利用RT-PCR分析了大菱鲆IgD重链基因的组织差异表达,结果显示,其在外周血白细胞、脾脏、前肾及中肾组织中具有较高的表达。将大菱鲆腹腔注射福尔马林灭活鳗弧菌后,实时荧光定量PCR检测其前肾组织中mIgD重链基因应答表达量,结果显示,mIgD重链基因在注射鳗弧菌后呈显著下调趋势,在注射后8h时降至最低值,其相对表达量约为对照组的1/28,然后呈逐渐恢复上调趋势,在48h时mIgD相对表达量与对照组无显著差异。  相似文献   

First-Order Derivatives of Principal and Main Invariants of Magnetic Gradient Tensor of a Uniformly Magnetized Tesseroid and Spherical Shell     

Deng  Xiao-Le  Shen  Wen-Bin  Yang  Meng  Kuhn  Michael  Ran  Jiangjun 《Surveys in Geophysics》2022,43(4):1233-1262

Surveys in Geophysics - Similar to the gravitational curvatures in the gravity field, the magnetic curvatures (i.e., third-order derivatives of the magnetic potential) have been recently proposed...  相似文献   

东太平洋赤道海域茎柔鱼(Dosidicus gigas)小型群体资源分布及其渔场环境特征     

玄文丹  崔国辰  李哲  魏裙倚  陶迎新  刘连为  陈峰  陈新军  朱文斌 《海洋与湖沼》2022,53(5):1234-1241

茎柔鱼(Dosidicus gigas)是我国远洋渔业的重要捕捞对象。当前针对茎柔鱼渔场分布及其与环境关系的研究多集中于秘鲁海域,而在赤道海域则研究较少。根据2019年12月至2020年2月在赤道海域获取的茎柔鱼生物学数据,以及同期的渔业生产和环境数据,运用胴长-体重关系拟合、Arcgis地统计插值、广义可加模型(GAM)探究其资源分布及渔场环境状况。结果表明:东太平洋赤道海域茎柔鱼胴长范围为136~407 mm,体重范围为117~1 557 g; 2019年12月~2020年4月各月渔获量呈先增加后减小趋势, 2月渔获量最高; CPUE (单位捕捞努力量渔获量)除2月增加外,总体呈下降趋势;渔场集中分布于0°~3°S、105°~114°W海域,不同月份渔场中心经向变化明显;渔场最适SST范围是24.5~25.5 ℃,最适chl a范围是0.16~0.20 mg/m³,月份是影响茎柔鱼CPUE的主要因子。进一步分析表明:该海域茎柔鱼渔获主要为小型群体;小型群体生长发育期(2~3月)对渔场分布有重要影响,生长发育期前茎柔鱼集群度高,生长发育期后逐渐分散活动;单一影响因子与茎柔鱼CPUE相关性不显著,综合考虑其他环境因素及其交互影响是今后的研究方向。  相似文献   

3种益生素配伍对异育银鲫(Carassius auratus gibelio)生长、消化及肠道茵群组成的影响   总被引：4，自引：1，他引：4