菱形藻株系MD1的脂肪酸组成及其在NaHCO3、NaCl和pH值影响下的生长特征     

苏娇娇  向文洲  萧邶  徐少琨  何慧 《热带海洋学报》2010,29(6)

研究了具有嗜碱特性的菱形藻Nitzschia sp.(MD1)株系的脂肪酸组分、含量以及不同NaHCO3、NaCl和pH值水平下对其生长的影响.GC-MS测定结果显示,在NaHCO3浓度为0.2mol·L-1、NaCl浓度为30g·L-1、初始pH值为9.5时,MD1的EPA含量占干重的3.02%,占总脂肪酸的比例为17.93%.生长测定结果表明,MD1具有极强的耐碱能力,其生长适宜的NaHCO3浓度为0.1-0.2mol·L-1,在0.3mol·L-1的NaHCO3浓度下仍能良好生长;MD1最适的起始pH值范围为7.5-9.5,其生长速率在pH7.5与pH9.5之间没有显著性差异(P>0.05),可以耐受pH值为11.5的高碱性环境并保持较好的生长;MD1显示广盐适应性,NaCl浓度在0g·L-1时获得最大比生长速率,在30-40g·L-1时对其生长没有显著影响(P>0.05),在NaCl浓度30g·L-1、pH 8.5-9.5及NaHCO3 0.2mol·L-1时,获得了最大比生长速率0.56d-1.由此可见,MD1可望具有进行大规模培养开发EPA的前景.  相似文献   

SESAM锁模激光器在卫星激光测距领域应用研究     

卫志斌  瞿锋  项清革  李谦 《测绘科学》2009,34(6)

本文在分析SESAM(Semiconductor Saturable Absorber Mirrors)锁模激光器原理及技术特点、描述其工作流程的基础上,展示了SESAM锁模激光器用于卫星激光测距的实测结果,通过对卫星激光测距数据数量及质量分析,得出了SESAM锁模激光器以窄脉冲宽度、高能量密度、输出稳定等优势首次在国内应用于卫星激光测距系统后,使系统整体性能得到明显提升的结论。  相似文献   

云参数法干旱遥感监测模型的完善   总被引：4，自引：0，他引：4  

刘良明  向大享  文雄飞  杨娜 《武汉大学学报(信息科学版)》2009,34(2)

在原有的云参数法干旱遥感监测模型的基础上,对云参数进行时间空间修正,得到了具有时间空间普适性的新型云参数法干旱遥感监测模型.首先分析了云参数随时空变化的规律即云参数影响函数,并分析确定修正函教的函数形式,然后联合遥感数据和同步地面实测20 cm深度土壤湿度数据确定修正函数,最后通过定权方式确定改进型干旱遥感监测模型.利用FY-2C数据进行了全国范围干旱遥感监测实验,同时与传统方法的监测结果进行对比分析,认为新型模型对空间时间大尺度条件下的干旱监测结果更准确可信.  相似文献   

The investigation on particulate organic carbon fluxes with disequilibria between thorium-234 and uranium-238 in the Prydz Bay, the Southern Ocean   总被引：1，自引：0，他引：1  

HE Jianhu  MA Hao  CHEN Liqi  XIANG Baoqiang  ZENG Xianzhang  YIN Mingduan  ZENG Wenyi 《海洋学报(英文版)》2008,27(2):21-29

Dissolved and particulate thorium-234, particulate organic carbon in the upper 150 m of water columns from five stations in the Prydz Bay, the Southern Ocean were determined during the 22nd Chinese National Antarctic Research Expedition （from November 2005 to March 2006 ）. The disequilibria between thorium-234 and its parent uranium-238 in upper layer was used to derive the averaged residence time of thorium-234, which decreased along with the latitude to the south and a minimum value, 1 - 8 d for particulate thorium-234 and 29 - 48 d for dissolved thorium-234, appeared at the medium latitude station, and the export fluxes of thorium-234 were calculated too and ＇a maximum value, 0. 35 -0. 63 Bq/（m^3 · d） for the particulate thorium-234 and 0. 44 -0. 65 Bq/ （m^3 ·d） for the dissolved thorium-234, appeared at the same station. The export fluxes of particulate organic carbon at different water columns were derived by two methods with irreversible scavenging model, and the averaged values were 104. 7 mmol/ （ m2 · d ） （ E method） and 120. 6 mmol/（ m2·d ） （ B method ）, respectively, indicating that a relatively high new production would exist in summer in the Prydz Bay where it will play a potential significant role in sequestering the absorption CO2 to deeper ocean.  相似文献   

中国与周边国家地缘经济关系的双向互动分析     

熊琛然  王礼茂  屈秋实  向宁  王博 《世界地理研究》2019,28(5):11-23

贸易关系可用来刻画中国与周边国家地缘经济关系的双向互动。本文通过构建源—汇互动矩阵分析框架，借助商品贸易数据，从出口与进口两个断面，选取偏好率、市场占有率和集中度3个指标分析了中国与周边国家地缘经济关系双向互动时序变化。结果表明：①周边国家出口到中国偏好率整体上升，且高于中国出口到周边国家偏好率；②中国出口到周边国家市场占有率远高于周边国家出口到中国市场占有率；③周边国家从中国进口偏好率高于中国从周边国家进口偏好率，中国从周边国家进口的偏好率主要集中在周边发达国家以及部分东盟国家；④中国从周边国家进口市场占有率总体上呈增长趋势，且高于周边国家从中国进口市场占有率；⑤中国与周边国家地缘经济关系双向互动主要集中在日本、韩国和新加坡等发展经济体以及东盟中的经济发展水平较高国家。周边国家和地区经济发展水平差异巨大，需要进一步探讨中国与周边国家和地区的地缘经济合作差异和演化规律，服务于“一带一路”建设和提升“周边是首要”的地缘边际效应。  相似文献   

三维激光扫描单点定位精度评定方法研究   总被引：3，自引：0，他引：3  

向娟  李钢  黄承亮  蒲玲媛 《海洋测绘》2009,29(3):68-70

三维激光扫描技术是当前的一种新技术，其仪器的精度评定方法还不完善，提出两种三维激光扫描单点定位精度评价方法，即外部符合精度评价方法和内部符合精度评价方法。介绍了两种方案的实施过程，并且通过实验进行了分析，得到了较好的评价结果。  相似文献   

Detection of the End Point Temperature of Thermal Denatured Protein in Fish and Chicken Meat Through SDS-PAGE Eiectrophoresis     

GAO Hongwei MAO Mao LIANG Chengzhu LIN Chao XIANG Jianhai 《中国海洋大学学报(英文版)》2009,8(1)

  相似文献   

Detection of the end point temperature of thermal denatured protein in fish and chicken meat through SDS-PAGE electrophoresis     

GAO Hongwei）  ）*  MAO Mao）  LIANG Chengzhu）  LIN Chao）    XIANG Jianhai）） Institute of Oceanology  Chinese Academy of Sciences  Qingdao  P. R. China ） College of Food Science  Engineering  Ocean University of China  P. R. China ） Sh  ong Entry-Exit Inspection  Quarantine Bureau of P. R. China  P. R. China 《中国海洋大学学报(英文版)》2009,8(1):95-99

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied in the detection of the end point temperature (EPT) of thermal denatured protein in fish and meat in this study. It was also used in studying the thermal denatured temperature range of proteins in salmon and chicken meat. The results show that the temperature ranges of denatured proteins were from 65 ℃ to 75 ℃ , and these temperature ranges were influenced by the processing methods. Through SDS-PAGE, the features of repeated he...  相似文献   

海水螺旋藻C-藻蓝蛋白富硒及其抗氧化特性   总被引：1，自引：0，他引：1  

成华  向文洲  吴华莲  彭娟  何慧  肖伟 《热带海洋学报》2008,27(5)

对海水钝顶螺旋藻Spirulina platensis C-藻蓝蛋白的富硒能力及含硒c-藻蓝蛋白对超氧阴离子(O2·)和羟基自由基(·OH)的清除作用进行了研究.结果表明,在添加低浓度硒(40-80mg·L-1)培养时,海水钝顶螺旋藻c.藻蓝蛋白对硒的富集效果显著强于淡水螺旋藻C-藻蓝蛋白.硒浓度为40mg·L-1时,C-藻蓝蛋白对硒的利用率最高,富硒系数最大(O.9%);硒浓度为60mg·L-1时,C-藻蓝蛋白含硒量最高(402mg·kg-1).含硒C-藻蓝蛋白比CJ藻蓝蛋白对超氧阴离子和羟基自由基的清除作用都有所加强,清除效应与C-藻蓝蛋白的含硒量及蛋白浓度呈正相关.C-藻蓝蛋白含硒量最高组(402mg·kg-1)在浓度为180μg·ml-1时,对超氧阴离子和羟基自由基的清除率分别可达到83%和35%,远高于同样条件下其他淡水种类相应蛋白的清除作用.研究结果显示,利用海水培养的螺旋藻能显著提高C-藻蓝蛋白的富硒能力和含硒C-藻蓝蛋白清除超氧阴离子的活性.此研究对开发具有抗氧化功能的含硒C-藻蓝蛋白显示出独特的技术优势和较大的应用潜力.  相似文献   

Molecular markers for identifying a new selected variety of Pacific white shrimp Litopenaeus vannamei     

于洋  张晓军  刘敬文  李富花  黄皓  李义军  刘小林  相建海 《中国海洋湖沼学报》2015,33(1):1-10

Selective breeding of the Pacific white shrimp Litopenaeus vannamei during the last decade has produced new varieties exhibiting high growth rates and disease resistance.However,the identification of new varieties of shrimps from their phenotypic characters is difficult.This study introduces a new approach for identifying varieties of shrimps using molecular markers of microsatellites and mitochondrial control region sequences.The method was employed to identify a new selected variety,Kehai No.1(KH-1),from three representative stocks(control group):Zhengda;Tongwei;and a stock collected from Fujian Province,which is now cultured in mainland China.By pooled genotyping of KH-1 and the control group,five microsatellites showing differences between KH-1 and the control group were screened out.Individual genotyping data confirmed the results from pooled genotyping.The genotyping data for the five microsatellites were applied to the assignment analysis of the KH-1 group and the control group using the partial Bayesian assignment method in GENECLASS2.By sequencing the mitochondrial control regions of individuals from the KH-1 and control group,four haplotypes were observed in the KH-1 group,whereas14 haplotypes were obtained in the control group.By combining the microsatellite assignment analysis with mitochondrial control region analysis,the average accuracy of identification of individuals in the KH-1group and control group reached 89%.The five selected microsatellite loci and mitochondrial control region sequences were highly polymorphic and could be used to distinguish new selected varieties of L.vannamei from other populations cultured in China.  相似文献