Development of a real-time PCR method for Thalassiosira rotula rapid detection     

HE Shanying  YU Zhigang  MI Tiezhu 《海洋学报(英文版)》2007,26(2):133-139

Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.  相似文献   

A STUDY OF MICROTEKTITES IN THE PACIFIC OCEAN     

彭汉昌  于众  缪昕  庄世杰 《海洋学报(英文版)》1984,3(1):106-113

In 1979, we found many small colorless transparent and light yellow-green glassy spherules in the surface layer and core sediment samples taken from the west of the Central Pacific Occan, which are considered to be microtektites. This finding is worthy of further determination of the range of strewn field and origin of tektites.  相似文献   

Analytically derived wind-wave directional spectrum part 1. Derivation of the spectrum     

Sheng-Chang Wen  Pei-Fang Guo  Da-Cuo Zhang 《Journal of Oceanography》1993,49(2):131-147

In contrast with the usual method to obtain the wind-wave directional spectrum by multiplying the frequency spectrum with an empirical directional function, the authors attempt to derive analytically the directional spectrum by adopting proper spectral form and using effective parameters, namely, the zero order momentm ₀ of the wind-wave frequency spectrumS(), its peak frequency ₀ and the so-called peakness factorP=₀ S(₀)/m ₀, where is angular frequency. The directional spectrum is given in a form of frequency spectrum for each direction. The spectral directionality depends on, in addition to frequency, the wind-wave growth status, for the peakness factorP as introduced by the authors previously is a measure of the wave development stage. The salient features of the directional spectrum, comparison with existing formulas and the verification of the spectrum by observational data are to be given in the Part 2 of the paper.Project supported by the National Natural Science Foundation of China.  相似文献   

Sediment Transport in Rivers and Coastal Waters     

YANG  Shu-qing 《中国海洋工程》2003,17(4):527-540

Following Bagnold's approach, a relationship between sediment transport and energy dissipation is developed. The major assumption made in the study is that the near bed velocity plays a dominant role in the process of sediment transport. A general relationship between energy dissipation and sediment transport is first proposed. Then the equations for total sediment transport are derived by introducing the appropriate expression of energy dissipation rate under different conditions, such as open channel flows, combination of wave and current, as well as longshore sediment transport. Within the flows investigated, the derived relationships are fairly consistent with the available data over a wide range of conditions.  相似文献   

半潜式超大浮体连接器动力特性的一种时间序列分析方法   总被引：1，自引：2，他引：1  

王璞  余澜 《海洋工程》2002,20(3):9-13

根据刚性模块弹性连接器 (RMFC)假设研究由 3个模块连接而成的半潜式超大浮体MobileOffshoreBase(MOB)的波浪载荷动力响应。模块和连接器动力响应根据时间序列法计算 ,这种方法包括海况模拟 ,波浪载荷计算和结构响应计算三个主要部分。通过对结构的适当简化 ,根据Airy波理论 ,Morrison公式以及流体中的结构运动方程、弹性连接器变形方程 ,计算求得MOB在不规则海况下的动力响应 ,并将其与相关文献中的结果进行比较 ,表明该方法有良好的精确性  相似文献   

扇贝提取物对实验性高脂血症大鼠血脂及脂质过氧化的影响     

毛文君  李翊  李八方 《海洋科学》2002,26(4):69-71

硒与心血管的结构、功能及其疾病的发生关系密切。世界卫生组织和国际原子能协会已把硒列为与冠心病发病有关的５种元素之一而加以研究。近年来 ,人们逐渐认识到脂质过氧化机理在动脉粥样硬化发生发展中的作用 ,体内血脂代谢异常及氧化损伤是动脉粥样硬化发生发展的重要原因。硒作为自由基清除剂在一定程度上 ,有调节血脂代谢及抗氧化的作用。饮食摄入一定量的硒和体内维持一定水平的硒 ,对防止高脂血症具有积极意义。扇贝提取物是以扇贝为原料提取制备的富含微量元素硒及多种微量元素、维生素和蛋白质等生理活性物质的富硒提取物。本研究利…  相似文献   

Theoretical wind wave frequency spectra in deep water——Ⅰ. Form of spectrum   总被引：1，自引：0，他引：1  

Wen Shengchang 《海洋学报(英文版)》1988,7(1):1-16

In this part ot the paper theoretical wind-wave spectra nave been derived oy (I) expressing the spectrum in series composed of exponential terms; (2) assuming that the spectrum satisfies a high order linear ordinary differential equation; (3) introducing proper parameters in the spectrum; and (4) making use of some known charateristics of wind-wave spectrum, for instance, the law governing the equilibrium range. The spectrum obtained contains the zero order moment of the spectrum m0, the peak frequency ω0 and the ratio R =ω/ω0 (ω being the mean zero-crossing frequency) as parameters. The shape of the nondimensional spectrum S(ω) = ω0S(ω)/m0(ω=ω/ω0) changes with R and theoretically reduces to a Dirac delta function δ(ω-1) when R = 1. A spectrum of simplified form is given for practical uses, in which R is replaced by a peakness factor P=S(1).  相似文献   

伶仃洋L＿2和L＿（16）孔第四纪有孔虫群与孢粉化石带特征及其地质意义   总被引：5，自引：0，他引：5  

陈木宏  赵焕庭  温孝胜  张乔民  宋朝景 《海洋地质与第四纪地质》1994,(1)

本文所依据的分析样品是取自珠江最大的一个河口湾伶仃洋中部的水下钻孔岩芯，其底部到达花岗岩基底。通过对第四纪有孔虫群与孢粉化石带特征的分析，阐明在相同的钻孔岩芯中，有孔虫分布变化所反映的古沉积环境特征与孢粉分析的古气候特征较为吻合，并与相应的沉积相对应，结合￣（１４）Ｃ和￣（２３０）Ｔｈ／￣（２３２）Ｔｈ比值法测年数据，从而较好地重塑本区晚更新世中期以来的地质历史。  相似文献   

不同季节的企鹅珍珠贝游离珠植核效果比较   总被引：7，自引：1，他引：7  

毛勇  梁飞龙  余祥勇  叶富良 《海洋通报》2003,22(6):88-91

在雷州流沙港湛江海洋大学珍珠研究室实验基地分别于春、夏和秋季进行了企鹅珍珠贝游离珠植核实验。其中，春季插核的死亡率最低，为15.1％--16．2％，留核率最高，为10％左右：而夏季插核的死亡率最高，为63．56％，留核率也仅为2．22％：秋季插核的留核率最低，仅收珠1颗，留核率为0．35％，死亡率也较高，为54.4％。结果表明，春季是企鹅珍珠贝较适宜的插核季节。  相似文献   

Parameterization of Wave Breaking Probability and Whitecap Coverage     

李晶于定勇  刘华兴 《中国海洋工程》2006,20(3):411-418

1 .Introduction Wave breaking and associated whitecapping have long beeninteresting due totheir close relationto many fields of ocean study,including air-sea interaction,remote sensing,ocean engineering,aswell as wave dynamics .The breaking probabilityBan…  相似文献