Molecular characterization and expression analysis of Lily-type lectin (SmLTL) in turbot Scophthalmus maximus,and its response to Vibrio anguillarum     

夏丹丹  马爱军  黄智慧  商晓梅  崔文晓  杨志  曲江波 《中国海洋湖沼学报》2018,36(2):508-518

A full-length lily-type lectin(Sm LTL) was identified from turbot(Scophthalmus maximus) in this study. By searching database for protein identification and function prediction, Sm LTL were confirmed. The full-length c DNA of Sm LTL is composed of 569 bp and contains a 339 bp ORF that encodes 112 amino acid residues. The Sm LTL peptide is characterized by a specific β-prism architecture and contains three mannose binding sites in a three-fold internal repeat between amino acids 30–99; two of the repeats share the classical mannose binding domain(Qx Dx Nx Vx Y) while the third binding site was similar to other fish-specific binding motifs(Tx Tx Gx Rx V). The primary, secondary, and tertiary structures of Sm LTL were predicted and analyzed, indicating that the S m LTL protein was hydrophilic, contained 5.36% α-helices, 39.29% extended strands, 16.07% β-folds, and 39.29% random coils, and three β-folds. Quantitative realtime polymerase chain reaction(qPCR) analysis revealed that the Sm LTL mRNA was abundantly expressed in skin, gill, and intestine. Low levels of Sm LTL expression were observed in other tissues. The expression of Sm LTL in gill, skin and intestine increased at m RNA level after stimulation of V ibrio anguillarum, our results suggest that Sm LTL serve as the first line of defence against microbial infections and play a pivotal role in the innate mucosal immune system. The current study indicates that Sm LTL is a member of the lilytype lectin family and the information reported here will provide an important foundation for future research on the role of this protein.  相似文献   

大菱鲆(Scophthalmus maximus)幼鱼生长性状的遗传力及其相关性分析   总被引：10，自引：1，他引：9       下载免费PDF全文

马爱军  王新安  杨志  曲江波  雷霁霖 《海洋与湖沼》2008,39(5):499-504

以4种不同地理群体大菱鲆为亲本，采用巢式设计方法和人工采卵授精技术，按照1雄配2雌的原则，构建了28个父系半同胞家系和56个母系全同胞家系，分别测定了每个母系生长到6月龄的全同胞个体60个后代的体长、全长、体高、体重，应用数量遗传学原理，利用全同胞资料，采用方差、协方差分析的方法，研究了大菱鲆6月龄生长性状的遗传力及性状间的遗传相关和表型相关。对性状遗传力研究结果表明，雌性遗传方差组分均大于雄性遗传方差组分，雌性遗传方差组分存在显著的母性效应。基于父系半同胞组内相关法估计的遗传力是大菱鲆体长、全长、体高、体重遗传力的无偏估计值，估计的遗传力准确可靠，估计值分别为0．282、0．251、0．283、0．450，为中高等遗传力，显示对大菱鲆生长性状进行选择育种具有很大的潜力。对性状间相关性分析结果表明，依据父系半同胞遗传协方差组分和表型协方差分别估计体长．全长、体长一体高、体长．体重、全长。体高、全长一体重、体高一体重间的遗传相关和表型相关，遗传相关在0．888-0．985，表型相关在0．864—0．957，各性状间遗传相关和表型相关经t检验均达到极显著水平（P〈0．01）。  相似文献   

七带石斑鱼（Epinephelus septemfasciatus）两个野生群体形态差异分析   总被引：5，自引：2，他引：3  

王新安  马爱军  陈超  杨志  曲江波 《海洋与湖沼》2008,39(6)

对2种不同地理种群七带石斑鱼进行14项形态性状的测定，采用2种多元统计分析方法和t-检验方法，比较了2个群体的外部形态特征。t-检验结果表明，2群体在14个性状中有12个性状表现出极其显著差异（P〈0．01），1个性状表现出差异显著（P〈0．05）。主成分分析构建了3个反映形态特征信息的综合性指标—主成分1、主成分2和主成分3，三者的贡献率分别为29．873％、25．091％和16．875％，三个主成分的累积贡献率为71．804％，2群体之间存在明显的偏离态势，形成两个不同的类群，2群体在主成分1轴上的差异较在主成分2轴上的差异更为明显，主成分1主要由经卜检验差异指标数量多和差异程度大的性状组成。判别分析表明，2个七带石斑鱼群体的形态差异显著（P〈0.0001），建立了2个群体的判别函数，其判别准确率及综合判别率均为100％，可以认为逐步判别法对七带石斑鱼不同群体的初步鉴定是可行的。差异系数（C.D）计算的结果表明，尽管2群体在形态上存在一定的差异，但尚未达到亚种水平。主成分分析、t-检验和判别分析的结论基本上是类似的，它们从不同的角度反映了群体间的形态学差异。  相似文献   

大菱鲆体重和形态性状的遗传动态分析     

王新安  马爱军  马得友 《海洋学报(英文版)》2015,34(2):55-62

In order to elucidate the genetic mechanism of growth traits in turbot during ontogeny, developmental genetic analysis of the body weights, total lengths, standard lengths and body heights of turbots was conducted by mixed genetic models with additive-dominance effects, based on complete diallel crosses with four different strains of Scophthalmus maximus from Denmark, Norway, Britain, and France. Unconditional genetic analysis revealed that the unconditional additive effects for the four traits were more significant than unconditional dominance effects, meanwhile, the alternative expressions were also observed between the additive and dominant effects for body weights, total lengths and standard lengths. Conditional analysis showed that the developmental periods with active gene expression for body weights, total lengths, standard lengths and body heights were 15–18, 15 and 21–24, 15 and 24, and 21 and 27 months of age, respectively. The proportions of unconditional/conditional variances indicated that the narrow-sense heritabilities of body weights, total lengths and standard lengths were all increased systematically. The accumulative effects of genes controlling the four quantitative traits were mainly additive effects, suggesting that the selection is more efficient for the genetic improvement of turbots. The conditional genetic procedure is a useful tool to understand the expression of genes controlling developmental quantitative traits at a specific developmental period(t-1→t) during ontogeny. It is also important to determine the appropriate developmental period(t-1→t) for trait measurement in developmental quantitative genetic analysis in fish.  相似文献   

都庞岭-九嶷山地区早元古代地壳存在证据     

彭学军  刘耀荣  李泽泓  郑基俭  马爱军 《华南地质与矿产》2005,(4):18-22

都庞岭-九嶷山地区出露的南华纪至第四纪地层最大厚度累加为18.5km,物探推测的地壳厚度为32~40km,两者相减尚应有13.5~18.5km厚的硅铝质地壳物质;地表基性-超基性火山岩中已发现有年龄值为1141~2707M a的片麻岩、麻粒岩、辉长岩、尖晶石二辉橄榄岩、片岩等沉积岩和岩浆岩变质结晶岩类;源岩主要属地壳成分的花岗岩,钕同位素t2DM值为1790~1380M a;花岗岩内磨圆状残留锆石SHRIMP法年龄值912~2669M a。推测南华系-奥陶系仅属上地壳基底,南华系之下还存在元古代甚至太古代的变质结晶基底。  相似文献   

转录组数据的大菱鲆(Scophthalmus maximus) SNP标记开发及多态性分析   总被引：1，自引：0，他引：1  

王婷  黄智慧  马爱军  马得友  王新安  夏丹丹  马本贺 《海洋与湖沼》2014,45(6):1300-1307

大菱鲆(Scophthalmus maximus)是最具商业价值和养殖前途的海水鱼类之一, 雌雄间生长有一定的差异。其高通量雌雄转录组测序的完成为大规模鉴定和开发SNP标记提供了参考序列。本研究基于大菱鲆雌雄转录组测序数据, 选择其中45个SNP位点, 设计引物63组, 其中21个位点(46.7%)应用小片段高分辨率熔解曲线(HRM)技术分型成功。对其进行多态性检测, 21个位点均具有二个单倍型。观测杂合度Ho的分布范围为0.256—1.000, 期望杂合度He的范围为0.276—0.518, 19个位点符合Hardy-Weinberg平衡。14个SNP位点位于基因编码区, 其中3个属于非同义突变。含有这些SNP位点的基因大多与信号转导和转录翻译相关。本研究结果表明, 高通量转录组测序和小片段HRM适合大规模SNP标记开发, 为大菱鲆的分子遗传育种提供了候选标记资源。  相似文献   

我国植物食性海水鱼类养殖发展前景     

丁福红  毛守康  雷霁霖  庄志猛  马爱军  徐宝荣 《海洋科学》2014,38(11):131

<正>近50年来,我国水产养殖业快速发展,从过去追求养殖面积扩大和养殖产量增加,向更加注重品种结构的调整和产品质量的提高。新的养殖技术和新的养殖品种不断推出,工厂化养殖、生态健康养殖模式迅速发展,2012年我国海水鱼养殖产量为102.84万t,年增长6.66%[1],品种达80余种。养殖模式方面,工厂化养殖、网箱养殖、池塘养殖等多种  相似文献   

大菱鲆（Scophthalmus maximus）大规模家系选育优良家系的生长发育规律   总被引：3，自引：1，他引：2       下载免费PDF全文

王新安  马爱军  黄智慧  刘庆明  郭黎  杨志  曲江波 《海洋与湖沼》2011,42(2):266-273

运用Logistic、Gompertz和von Bertalanffy3种生长模型分别对大菱鲆的5个优良家系E♂1×E♀2、F♂4×N♀3、F♂2×E♀4、E♂2×F♀1、F♂1×F♀4及对照组群体的生长曲线进行了拟合和比较分析。结果表明,家系E♂1×E♀2、F♂2×E♀4和E♂2×F♀1的最优拟合模型为Gompert...  相似文献   

云纹石斑鱼（Epinephelus moara）早期发育阶段的摄食与生长特性   总被引：2，自引：0，他引：2  

陆丽君  陈超  马爱军  翟介明  王新安  李伟业 《海洋与湖沼》2011,42(6):822-829

采用随机取样实验生态学方法,对人工培育的云纹石斑鱼的仔、稚、幼鱼各发育阶段的形态特征及摄食变化状况进行了观察和描述。结果表明,云纹石斑鱼仔、稚鱼生长期间,分别以牡蛎幼体、轮虫、卤虫为饵料,幼鱼摄食配合饲料。形态学数据测量结果表明,云纹石斑鱼仔、稚、幼鱼的全长、体重和日龄三者之间存在相关关系。全长与日龄的关系式为L=2....  相似文献   

Turbot Scophthalmus maximus：stocking density on growth, pigmentation and feed conversion   总被引：1，自引：1，他引：0  

马爱军 陈超雷霁霖  陈四清庄志猛 王印庚 《中国海洋湖沼学报》2006,24(3):307-312

1 INTRODUCTION Turbot is a type of marine flatfish naturally distributing along the coastal area of European. It is highly demanded for good taste. The price therefore, is very expensive. To meet the consump- tion, large-scale turbot cultures were perform…  相似文献