Based on the three-dimensional digital image correlation (3D-DIC) technique, the stereovision system has been applied to the improved triaxial apparatus to obtain 3D full-field deformation of the specimen during triaxial testing. Through the calibration process, the 3D-DIC technique can obtain the accurate specimen’s spatial displacement deformation. Meanwhile, a subpixel edge detection algorithm has been combined with 3D-DIC technique to calculate the radial strain and the volume strain of the specimen directly. Furthermore, a series of consolidated drained and undrained triaxial tests were carried out on Hainan (China) sand specimens and measured by the conventional and the image measurement methods. Compared to the results measured by the conventional method, the image measurement technique can obtain the more experimental data, such as the 3D displacement field of the whole specimen, the local strain distribution, and so on. The measurement results also show the conventional method would be disturbed by the end constraints in triaxial tests so that the strength of the soil would be overestimated. Meanwhile, the middle of the specimen would be selected to calculate the stress–strain relationship without the influence of the end constraints in the proposed method. Based on the image measurement results, the proposed method has the potential to be used in geotechnical tests for exploring the soil’s progressive failure behaviors, inhomogeneous deformation and mechanical characteristics.
Planktonic bacteria are abundant in the Chukchi Borderland region. However, little is known about their diversity and the roles of various bacteria in the ocean. Seawater samples were collected from two stations K2S and K4S where sea ice was melting obviously. The analysis of water samples with fluorescence in situ hybridization (FISH) showed that DMSP-degrading bacteria accounted for 13% of the total bacteria at the station K2S. No aerobic anoxygenic phototrophic (AAP) bacteria were detected in both samples. The bacterial communities were characterized by two 16S rRNA gene clone libraries. Sequences fell into four major lineages of the domain Bacteria, including Proteobacteria (Alpha, Beta and Gamma subclasses), Bacteroidetes, Actinobacteria and Firmicutes. No significant difference was found between the two clone libraries. SAR11 and Rhodobacteraceae clades of Alphaproteobacteria and Pseudoalteromonas of Gammapro-teobacteria constituted three dominant fractions in the clone libraries. A total of 191 heterotrophic bacterial strains were isolated and 76% showed extracellular proteolytic activity. Phylogenetic analysis reveals that the isolates fell into Gammaproteobacteria, Bacteroidetes, Actinobacteria and Firmicutes. The most common genus in both the bacterial isolates and protease-producing bacteria was Pseudoalteromonas. UniFrac data showed suggestive differences in bacterial communities between the Chukchi Borderland and the northern Bering Sea. 相似文献
