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101.
Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection. 相似文献
102.
中国第22次南极科学考察(2005年11月至2006年3月)期间,测定了南极普里兹湾海域5个站位的从表层至150 m水深的不同层位水样中溶解态和颗粒态234Th,238U的放射性比活度以及颗粒有机碳.利用234Th/238U在上层水体中的不平衡,计算了南极普里兹湾上层水体中234Th的平均停留时间和输出通量.结果显示,随着纬度的增加,上层水体中颗粒态和溶解态234Th的平均停留时间总体趋向减小,并在中纬度站位出现了最低值,分别为1~8和29~48 d,而颗粒态和溶解态234Th的输出通量则在中纬度站位出现了最大值,分别为21~38和26~39 dpm/(m3·d).运用箱型清除模式,利用两种不同的方法估算了各水柱中从真光层底部输出的POC通量,平均值分别达到104.7 mmol/(m2·d)(E法)和120.6 mmol/(m2·d)(B法),表明南极普里兹湾夏季存在很高的新生产力,它将会对该海域碳的生物泵过程产生重要作用. 相似文献
103.
区域海洋地质数据库建设纲要 总被引:5,自引:0,他引:5
区域海洋地质数据库是中国地质调查局基础数据库的一个重要组成部分,是实现地质调查基础性与公益性战略部署的依托。根据区域海洋地质数据库建设的目标与任务,在数据结构和实施步骤等方面进行了初步研究。区域海洋地质数据库及其管理信息系统适合采取基于Web系统的三层体系结构,在数据构成上亦分为区域海洋地质调查原始资料与成果资料,其中元数据库的建设对数据库的应用非常重要,用户可以通过元数据了解数据的存在性、适宜性、有效性等,确定数据的使用方法,因此,元数据库的建设应采用国际已经普遍认可的ISO/TC211发布的ISO19115空间元数据标准。 相似文献
104.
Based on Hong‘s theory, previous random models, and a generalized expression suitable for FIT calculation, the interaction between irregular waves and vertical walls is numerically simulated. The results of simulation demonstrate that the wave energy changes with the incidence angle and the distance from the wall. Particularly, the Mach effect and the combined wave spectrum characteristics are analyzed in detail, which are significant in both theory and practice. 相似文献
105.
Analysis of Dynamic Characteristics of Submarine Free Spanning Pipelines by Complex Damping Method 总被引:2,自引:0,他引:2
Considering the effect of the internal flowing fluid and the external marine environmental condition, the differential equation for the vortex-induced vibration (V1V) of the free spanning pipeline is derived and is discretized by the Hermit interpolation function. The free vibration equation with the damping term is solved by the complex damping method for the natural frequency, and then the effect of fluid damping on the natural frequency of the free spanning pipeline is analyzed.The results show that fluid damping has a significant influence on the damped natural frequency of the free spanning pipeline in the lock-in state, while it has little influence when the pipeline is out of the lock-in state. In the meantime,the change of the free span length has the same effect on the damped natural frequency and the undamped natural frequency. 相似文献
106.
An optimization mathematical model of the pile forces for piled breasting dolphins in the open sea under various loading conditions is presented. The optimum layout with the well distributed pile forces and the least number of piles is achieved by the multiplier penalty function method. Several engineering cases have been calculated and compared with the result of the conventional design method. It is shown that the number of piles can be reduced at least by 10%—20% and the piles‘ bearing state is improved greatly. 相似文献
107.
Zhang Changkuan Wang Zhen Zhang Dongsheng
Associate Professor Dept. of Navigation Ocean Engineering Hohai University Nanjing
Lecturer Dept. of Navigation Ocean Engineering Hohai University Nanjing
Professor Dept. of Navigation Ocean Engineering Hohai University Nanjing 《中国海洋工程》1995,(2)
A numerical model of shoreline change of sand beaches based on long-term field wave data is proposed, the explicit and implicit finite difference forms of the model are described, and an application of the model is presented. Results of the application indicate that the model is sensitive to the order of the input wave data, and that the effects of long-term wave series and the effects of the mean annual wave conditions on the model are different. Instead of a single wave condition, the wave series will make the calibration and the verification of the model more practical and the results of the model more reasonable. 相似文献
108.
Liu Shuxue Yu Yuxiu
Assistant Researcher State Key Laboratory of Coastal Offshore Engineering Dalian University of Technology 《中国海洋工程》1995,(1)
The directional spectrum is one of the basic characteristics of sea waves. The observations of directional spectrum of sea waves were successfully conducted at platform Bohai 8 during 1991 and 1992 using a wave gage array for the first time in China. Based on the field data, the directional spectrum which depends on the wave growth is given in this paper. Before observations, the effects of the type of gage array, the distance between the gages and the platform itself on the measured results and the precision of some methods for estimating the directional spectrum were investigated and compared with the methods of numerical simulations and model tests of multi-direcitonal irregular waves. This ensures the quality of the observations and estimations of the directional spectrum. 相似文献
109.
阐述把维生素B12生产菌添加到培养水中培养褶皱臂尾轮虫BrachionusPlicatilis的买验。共18株细菌分离于轮虫培养池,其中,有一株产维生素B12的假单胞杆菌TP4对轮虫的生长繁殖有明显的促进作用。把TP4菌株培养后,加入到2L的烧杯和500L的水槽中培养泰国S型轮虫时.在9d(天)和6d(天)中,轮虫密度从124~139和242~288个体/ml增殖到4,417~5,540和1,017~1,254个体/ml,分别比对照组增加了4~6及2~3倍。 相似文献
110.
我国海域分等定级指标体系研究 总被引:3,自引:0,他引:3
长期以来,海域的无偿使用导致海洋开发活动秩序混乱、海洋环境污染加剧、海洋生物资源枯竭、海洋生态环境遭到破坏,严重阻碍和制约沿海地区的可持续发展.本文试图根据经济学的“地租级差”理论和运筹学中的“层次分析法”并结合我国沿海11个省、市和自治区的社会、经济、基础设施、自然资源等因素进行理论探讨,给出了我国管辖海域的等级.为今后我国制定海域有偿使用金的征收标准奠定基础. 相似文献