海带雌性原生质体的分离与培养     

秦松  王希华  童顺  曾呈奎 《海洋与湖沼》1995,26(S1):126-129

于1993年1月 - 1993年4月，为了制备遗传性纯一的基因工程受体，用酶法从海带雌配子体单性生殖系分离雌性单倍体原生质体。从皱纹盘鲍消化腺及消化器官提取出鲍酶，在25°C下、1％（W／V）的鲍酶与2％（W／V）的纤维素酶（Onozuka R－10）合用能有效解离单细胞和原生质体。海带雌性原生质体经2d培养再生了细胞壁。  相似文献   

薛家岛湾沉积动力学特征及海港开发研究   总被引：3，自引：0，他引：3  

李凡  林宝荣  吴永成  王从敏 《海洋与湖沼》1994,25(4):452-457

于１９９０年秋对胶州湾内的薛家岛湾进行海洋水动力条件及积动力学特征,泥沙运动规律研究,为开发建设港口提供科学依据。结果表明,湾内深大于１ｍ的海底泥沙发生“全面移动”的时间,一年内仅有１－２ｄ,其余处于稳定状态或“表面移动”状态。湾内泥沙来源很少,预计建港后,港池和航道年平均回淤量分别小于１６ｃｍ,１０ｃｍ对于开发中小型港口十分有利。  相似文献   

Three-dimensional numerical prediction of vertical temperature structure of the Huanghai and the Bohai Seas     

Jin Meibing  Wang Zongshan  Xu Bochang 《海洋学报(英文版)》1993,12(4):511-520

In the paper, the sea is divided into two layers with density jumping, assuming that the physical parameters in each layer are independent of depth. Two-layer flow field with tide and wind currents is calculated with extended ADI method, after the calculation for flow field is stable , coupled with temperature diffusion equations and thermohaline depth prediction equation, a four-day time prediction of the surface, bottom temperature and thermohaline depth of the Huanghai and the Bohai Seas. At the same time, three dimensional temperature field of sea water is predicted through vertical temperature distribution function. The result indicates that the prediction quality of the whole model and the fitting degree between the predicted result and the measured values are satisfactory.  相似文献   

Impact Buckling and Post-Buckling of Elasto-Viscoplastic Cylindrical Shell Under Torsion     

Wang Deyu Chen Tieyun Associate Professor 《中国海洋工程》1997,(1)

The dynamic torsional buckling and post-buckling of elasto-plastic circular cylindrical shell isstudied.By the usage of the Bodner-Partom constitutive relation,the present visco-plastic problem is treat-ed as an orthotropic elastic problem.Based on the nonlinear large deflection shell therory,the governingdynamic buckling equation is solved by the Runge-Kutta method,and the critical buckling stress is deter-mined by the B-R criterion.The present paper is mainly focused on the imperfection sensitivity,the strainrate sensitivity,and the dynamic post-buckling characters for the shell loaded dynamically with a constantshear strain rate.Numerical results show that the critical shear stress and imperfection sensitivity will in-crease with the increase of strain rate.It is found that the elastic buckling stress is more sensitive toimperfection than the visco-plastic buckling.The present research also reveals some dynamic post-bucklingcharacters for the cylindrical shell,and it is shown that the shell will vibra  相似文献   

扇贝的养殖环境及其体内的细菌学分析   总被引：1，自引：0，他引：1  

宋庆云  罗挽涛  王文兴  薛清刚 《海洋科学进展》1997,(3)

自1992年9月～1994年11月，对青岛胶州湾养殖的扇贝及环境中的细菌组成和某些生态学特性进行了调查。养殖区表层海水中异养菌和弧菌平均数各波动于（21．2～39．7）×102／cm3和（7．3～19．2）×102／cm3之间。其中1号和4号站位的菌数比其它越位都高，这和它们靠近码头，受到附近的排水污染有关。在扇贝的消化盲囊和性腺内也分离到异养菌和弧菌，分别为103～105个g和103～105个／g。这些细菌可能因扇贝遇到环境改变防御能力下降时，扩散到其它器官并大量繁殖，因而造成感染而死亡。从对分离菌的药物敏感试验结果看，近年一些菌株对原来敏感的药物产生了抗药性，如四环索和链霉素，今后在育苗过程中避免滥用抗菌药物是十分重要的。  相似文献   

大深度触底指示器Pinger换能器及其声反射系统     

王清池  许肖梅  许鹭芬  黄锡明 《海洋科学》1997,21(6):61-63

  相似文献   

副热带海域的海洋加热异常及其对副高的影响     

陈永利  赵永平  王爱莲 《海洋科学集刊》1997,(38):79-86

海洋在天气、气候及大气环流变化过程中起着重要作用,海洋热源的异常分布是造成长期天气异常的重要原因。海洋对大气的作用主要是通过海气界面的热量交换来实现的，因此,直接运用海气热量交换来考虑海洋对大气的加热作用,分析海洋加热异常对长期天气、气候及大气环流的影响,具有更明确的物理意义。 西北太平洋副热带高压是造成我国旱、涝及天气变化的主要副热带天气系统,对我国夏季气温、雨带位置及强度影响很大。西北太平洋中、低纬副热带地区是海洋向大气提供热量和水汽较多的地区,也是海气相互作用最强烈的海域,这一地区的海洋加热对西北太平洋副热带高压的变化将有直接影响。本文分析了冬季西北太平洋副热带海域海洋加热主要场的分布特征,指出了海洋加热与西北太平洋副热带高压的关系。结果表明,冬季该海域海洋加热较常年偏多时,冬、春至初夏副高偏弱,位置偏东、南(6月副高脊线位置除外);反之,海洋加热较常年偏少时,副高偏强,位置偏西、北(6月副高脊线位置除外)。该海域海洋加热对副热带地区大气环流,尤其是副热带高压环流的变化有着重要作用。本结果对预测西北太平洋副热带高压的变化,研究中纬度海洋加热对西北太平洋副热带高压及其大气环流的影响有十分重要的意义。  相似文献   

Shear Failure of a Clamped Dented Tubular Beam Under Lateral Impact   总被引：1，自引：0，他引：1  

Wang Deyu  Zhang Shanyuan Associate Prof.  Depart  of Naval Archi.  Ocean Engineering  Shanghai Jiaotong University  Shanghai Prof.  Research Institute of Applied Mechanics  Taiyuan University of Technology  Taiyuan 《中国海洋工程》1996,(2)

- The shear failure of a rigid-plastic dented clamped tubular beam under the lateral impact of a mass is investigated. Both the denting and the impact point are in the middle span of the beam. It is assumed that denting does not spread during the shear sliding. Numerical results show that the axial force and lateral deflection of the beam are very small at the moment of the occurence of shear failure, which means that the finite deformation effect can be neglected in the shear failure analysis. Also, some aspects of the initial impact energy are investigated.  相似文献   

大鹏澳海域水体磷的形态及其分布特征     

彭云辉  王肇鼎 《热带海洋学报》1996,(2)

根据１９９２年１２月（冬季）和１９９３年７月（夏季）对大鹏澳海域２４ｈ定点连续观测和夏季大面调查，统计了该海域各种形态磷的变化范围和平均含量；计算了各种形态磷分别占总磷（ＴＰ）和总溶解磷（ＴＤＰ）的百分比；讨论了各种形态磷之间的相互关系；分析了影响ＴＰ和ＴＤＰ分布的环境因素。  相似文献   

Cross-equatorial flow and its effects on the hurricanes occurrence over the Southern Hemisphere     

Qian Ping  Wang Jingyi 《海洋学报(英文版)》1989,8(2):307-313

Channels of cross - equatorial flow and their characteristics Winds at every 5 deg. grid point from 30°E to 180° over the equator at 850 hPa and 200hPa from December 1984 to February 1985 have been separated into U(zonal wind) andV(meridional wind).The V component has been used to represent cross- equatorial flow. FromFig. 1 we obtained that as during the Northern summer,there are also severa1 regions in which  相似文献