Isolation of CYP17 Ⅰ, 3β-HSD and AR Genes from Spotted Sea Bass (Lateolabrax maculatus) Testis and Their Responses to Hormones and Salinity Stimulations     

CHI Meili  NI Meng  JIA Yongyi  GU Zhimin  WEN Haishen 《中国海洋大学学报(英文版)》2019,(2):420-430

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Assessing effects of “source-sink” landscape on non-point source pollution based on cell units of a small agricultural catchment     

WANG Jin-liang  CHEN Cheng-long  NI Jiu-pai  XIE De-ti  GUO Xi  LUO Zhi-jun  ZHAO Yue 《山地科学学报》2019,(9)

Ascertaining the relationship between "source-sink" landscape and non-point source(NPS) pollution is crucial for reducing NPS pollution, however, it is not easy to realize this target on cell unit scale. To reveal the relationships between "sourcesink" landscape and NPS pollution based on cell units of a small catchment in the Three Gorges Reservoir Region(TGRR), the runoff and nutrient yields were simulated first by rainfall events on a cell unit scale based on the Annualized AGricultural Non-Point Source Pollution Model(AnnAGNPS). Landscape structure and pattern were quantified with "sourcesink" landscape indicators based on cell units including landscape area indices and locationweighted landscape indices. The results showed that:the study case of small Wangjiagou catchment highlighted a good prediction capability of runoff and nutrient export by the AnnAGNPS model. Throughout the catchment, the spatial distribution trends of four location-weighted landscape indices were similar to the trends of simulated total nitrogen(TN) and total phosphorus(TP), which highlighted the importance of spatial arrangement of "source" and "sink" landscape types in a catchment when estimating pollutant loads. Results by Pearson correlation analysis indicated that the location-weighted landscape index provided a more comprehensive account of multiple factors, and can better reflect NPS-related nutrient loss than other landscape indices applied in single-factor analysis. This study provides new findings for applying the "source-sink" landscape indices based on cell units in small catchments to explain the effect of "source-sink" landscape on nutrient export based on cell unit, and helps improve the understanding of the correlation between "source-sink" landscape and NPS pollution.  相似文献   

产新琼四糖的海洋微泡菌属AG1热稳定性β-琼胶酶的过量表达与鉴定     

朱艳冰  高贺  李鹤宾  倪辉  姜泽东  李利君  肖安风 《海洋学报(英文版)》2019,38(2):96-106

从微泡菌属AG1（Microbulbifer sp. AG1）克隆得到1302 bp大小的琼胶酶基因,该基因编码产物为一个成熟蛋白（413个氨基酸残基）外加一个信号肽（20个残基）。将不含信号肽片段的琼胶酶在E. coli BL21 （DE3）中进行了异源表达和纯化。使用琼脂糖作为底物,该重组琼胶酶的最适反应温度和pH分别为60℃和7.5。该重组酶表现出优良的热稳定性,在50℃和60℃下处理1 h,重组琼胶酶仍能分别保持67%和19%的残余酶活力。除了SDS,重组琼胶酶对于其他测试的抑制剂、去垢剂和尿素变性剂有着较好的抗性。利用薄层色谱和以对硝基苯-α/β-D-吡喃半乳糖苷为底物的酶活力分析结果表明,该重组琼胶酶为β型琼胶酶,它水解琼脂糖的主要终产物为新琼四糖,而且不同聚合度的酶解产物具有抗氧化活性。  相似文献   

GPS/PWV时间序列特征提取方法的研究     

胡广保  叶世榕  张彦祥  夏朋飞  夏凤雨 《大地测量与地球动力学》2019,39(1):36-40

针对GAMIT/GLOBK软件解算得到的4 a GPS/PWV时间序列的特征提取问题,提出基于滤波辅助的部分集成经验模态分解（PEEMD）与Hilbert谱分析相结合的特征提取方法。首先,在PEEMD方法的基础上,结合滤波辅助的PEEMD方法与Hilbert谱分析,建立GPS/PWV时间序列特征提取模型;然后,将所提出的方法应用于TNML测站4 a的GPS/PWV长时间序列和7 d的GPS/PWV短时间序列分析中,并将滤波辅助的PEEMD结果与传统的小波分解结果进行对比。结果表明,该特征提取方法能准确有效地提取出 GPS/PWV时间序列中的周年周期和日周期特征分量,滤波辅助的PEEMD分解结果与小波分解结果一致,且提取的特征分量与原始信号更加吻合。  相似文献   

基于渔获群落多样性指标的深水流刺网渔具作业性能分析   总被引：1，自引：0，他引：1  

徐国强  李鹏飞  张洪亮  李德伟  朱文斌 《海洋湖沼通报》2019,(2):70-80

根据在东海区开展的3种型深水流刺网选择性试验数据,对3种型深水流网渔获组成、多样性及渔获性能进行了分析比较研究。结果表明:(1)本次试验共捕获33种渔获物,包括鱼类28种、头足类3种、甲壳类2种,其中3种型刺网均捕获种类8种;(2)3种型刺网优势种存在差异,日本方头鱼和黄鲷为共同优势种;(3)3种型刺网捕获种类多样性指数(D值、H’值、J’值)均不存在显著性差异(P>0.05),但渔获种类差异明显,3种型刺网两两比较种类Sorensen相似度均低于50%;(4)同型刺网相邻网目尺寸Bray-Curtis相似性较高,随着网目间距增加,相似度逐渐减低,以重量和尾数计算的相似性基本趋于一致;(5)3种型刺网随网目尺寸的增加(50～70mm),其W统计值(-0.113～0.040)总体上呈现依次增大的趋势,干扰程度逐渐较低。从生态多样性保护角度,增大网目尺寸是目前保护东海区底层生物多样性最有效的方式之一。  相似文献   

北斗三号系统与GPS定位性能仿真对比分析     

倪煜淮  许杰  邹永刚  李厚朴 《海洋测绘》2019,39(4):71-74

为了解北斗三号系统在国内外及南北极地区的定位性能,在STK仿真平台下建立了北斗三号系统30颗星(3GEO、3IGSO和24MEO)的星座模型,对北京、三亚、新加坡、南北极点等11个站点的平均可见星数和几何精度因子GDOP值进行了分析,并与GPS进行了对比。结果表明,北斗三号系统较GPS在国内外及南北极地区典型站点的定位性能更优。  相似文献   

基于点云数据的改进四叉树空间索引研究     

刘中华  李鹏飞  许章平 《测绘与空间地理信息》2019,42(5):38-39,42,47

随着测绘行业的不断发展,三维激光扫描技术已经成为地理信息产业中不可或缺的重要组成部分^[1],然而点云庞大的数据量给处理带来了一定的困难。本文针对运用车载移动测量系统所获取的海量点云数据,采用了一种基于编码改进四叉树索引的点云数据组织处理方法。结果证明该方法提高了创建索引的速度,减少了树的深度以及数据的冗余量,并基于此编码进行最邻近查找,具有可行性和有效性。  相似文献   

多维度动态农村土地承包经营权业务权限的设计与实现     

万术海  倪慧珠  王飞龙  刘校妍  张傲敏 《测绘与空间地理信息》2019,42(3):152-155,161

为满足浙江省各级农经管理部门在农村土地承包经营权管理系统中复杂的权限管理需要,基于RBAC模型实现了以Apache Shiro安全框架为支撑的多维度动态业务权限管理。该模型从角色维度、部门维度、空间维度、时间维度上分别对用户的资源菜单、工作流、数据操作空间、数据历史回溯4个方面的权限进行细粒度控制。在浙江省农村土地承包管理信息系统中实际运行表明,设计的业务权限系统可满足省市县各级农经管理部门在管理系统的权限要求。  相似文献   

Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses     

YOU Feng  WANG Wei  XU Dongdong  ZHU Xiangping  NI Jing  WU Zhihao  XU Yongli  WANG Xincheng  ZHANG Peijun 《中国海洋湖沼学报》2009,27(2)

The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder Sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes,12 confirmed hybridization of the paternal and matemal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.  相似文献   

ETM遥感图像在措勤东部第四系含水性研究中的应用     

赵银兵  何政伟  倪忠云 《测绘科学》2009,34(5)

措勤东部属于极端缺水的地区,利用遥感技术开展第四系松散堆积物含水性调查具有重要作用。本文选用ETM753与8波段融合图像作为遥感解译底图,结合地形地貌、地质和气象影响,建立残积、冲积、洪积、冲洪积、冰川永久积雪、湖积、沼泽和坡积的识别知识体系,划定第四系松散堆积物的高、中、低、差四个含水等级,利用G IS实现第四系松散堆积物及其含水性的高程、坡度和坡向分布,为区内水资源的深入调查提供有力支持。  相似文献