不同年度收存的褶皱臂尾轮虫休眠卵的孵化及滞育胚胎“激活”效果的研究   总被引：3，自引：0，他引：3  

周利  相建海  郑严 《海洋与湖沼》2002,33(6):648-656

比较了 1 985— 1 998年间由同一褶皱臂尾轮虫 (Brachionusplicatilis)种群在实验室内历年产生并收存的休眠卵的孵化效果 ,并初步探讨了胚胎“激活”处理对孵化的影响。结果表明 ,休眠卵的收存年度或时间对其在一定孵育期内的总孵化率 (THR)和日孵化率 (DHR)有极显著影响 (x2 检验 ,p<0 .0 0 1 ) ,但THR和DHR与储存时间间均无显著的相关性 ,而是呈极大波动。同时发现“激活”作用具有促使孵化高峰提前到来、增强孵化同步性的双重功效 ,从而可明显缩短孵育时间 ,提高孵化速率。但“激活”前后多数卵样的总孵化率无显著变化 (p >0 .0 5 )。详细讨论了可能导致孵化率波动的多种因素 ,例如遗传变异、培养历史、储存及孵化的环境条件等。  相似文献   

山东近海牙鲆(Paralichthys olivaceus)自然和养殖群体10个微卫星基因座位的遗传多态性分析   总被引：26，自引：7，他引：26  

王伟  尤锋  高天翔  张培军 《海洋与湖沼》2004,35(6):530-537

采用微卫星遗传标记技术对山东近海牙鲆自然群体和养殖群体的遗传多样性进行了研究。实验所用牙鲆于2003年5月分别采自山东近海和青岛胶南养鱼场，各20尾，取全血或肌肉组织，以酚-仿抽提方法提取基因组DNA，利用筛选获得的10对微卫星引物进行PCR扩增，反应产物经8％非变性聚丙烯酰胺凝胶电泳分离、EB显色，用ImageMasterl D Elite(Version 3．01)软件分析电泳结果，并计算了相应的遗传学参数。结果表明，在自然和养殖群体中，10个基因座位的等位基因平均数(α)分别为6．7和6．1，每个基因座位有效等位基因数(αe)分别为1．8—6．8和2．5—6．7，群体平均杂合度(H)分别为0．8120和0．7310；两个群体间的遗传相似性系数、遗传距离和基因分化系数为0．8558、0．1557和0．0558；自然群体内每个座位上的多态信息含量(PIC)为0．59—0．84、个体识别率(DP)为0．54—0．86、非父排除率(PPE)为0．41—0．72，其累积个体识别率和非父排除率均达到0．9999，表明所选座位属中高识别力的遗传标记，可以将它们应用于今后牙鲆雌核发育群体的遗传变异分析以及进一步的遗传育种的研究中。  相似文献   

Photoinduced toxicity single and binary mixtures of four polycyclic aromatic hydrocarbons to the marine diatom Skeletonema costatum   总被引：1，自引：0，他引：1  

MENG Wei  WANG Liping  ZHENG Binghui 《海洋学报(英文版)》2007,26(6):41-50

Diatom Skeletonema costatum Cleve is one of the main predominant phytoplankton species in the Changjiang Estuary in China. In order to provide some basic information for future assessment of the potential risk on phytoplankton communities in this estuary caused by polycyclic aromatic hydrocarbons(PAHs), this alga was selected as a representative to investigate the photoinduced toxicity of PAHs, in single and mixture. Four PAHs including three-ring phenanthrene and anthracene, four-ring fluoranthene and pyrene were tested in the laboratory. The single toxicity of each PAH on this microalga was compared with and without the simulated solar UV radiation. The results showed that this microalga was sensitive to PAH's photoinduced toxicity. Ratios of the 72 h median effect concentration obtained for fluorescent and UV light tests were about 8.4 for phenanthrene, 13.0 for anthracene, 6.5 for fluoranthene, and 5.7 for pyrene, indicating that UV light enhanced the PAH toxicity to this alga significantly. Under the fluorescent radiation (lacking UV), the dose-response curves based on chemical concentrations revealed that the order of toxic strength was fluoranthene greater than pyrene greater than anthracene greater than phenanthrene; while under the UV radiation (476 μW/cm2 for UVA, 6.5 μW/cm2 for UVB) it became fluoranthene approximately equaling anthracene greater than pyrene greater than phenanthrene, indicating that the UV light also changed its relative toxicity to this alga. The photoinduced toxicity of PAHs to the marine diatom S. costatum might be a synergistic effect of photosensitization reactions (e.g., generation of single-state oxygen) and photomodification (photooxidation and/or photolysis).The combined effects of six binary mixtures on the marine diatom S. costatum were investigated using the additive-index method. Four binary-mixtures (phenanthrene plus anthracene; phenanthrene plus pyrene; anthracene plus fluoranthene; anthracene plus pyrene) were found to be synergistic joint action mode, while two binary-mixtures (phenanthrene plus fluoranthene; fluoranthene plus pyrene) displayed antagonist, revealing a complex pattern of possible interactions. The combined action mode of PAHs might be related to various factors such as the mixture compounds, mixture ratios and test conditions, etc.  相似文献   

软粘土中插入式大直径薄壁圆筒结构的一种极限状态分析模型     

王乐芹  周锡礽  张伟 《海洋技术学报》2005,24(1):95-100

根据插入式大直径薄壁圆筒结构的工作特点建立了其合理的破坏机制，以此为基础建立了受横向荷载作用的插入式大直径薄壁圆筒结构的上限法极限分析模型，从中可以得到作用于插入式大直径薄壁圆筒结构上的横向极限荷载近似的上限解；并根据上限法模型计算结果，提出了计算受横向荷载作用的插入式大圆筒结构侧壁极限抗力的简化修正模型，利用此修正模型，基于极限平衡法，可以求解作用于插入式大直径薄壁圆筒结构上的横向极限荷载。  相似文献   

Application of LICOM to the numerical study of the water exchangebetween the South China Sea and its adjacent oceans   总被引：8，自引：1，他引：8  

CAI Shuqun  LIU Hailong  LI Wei  LONG Xiaomin 《海洋学报(英文版)》2005,24(4):10-19

1 IntroductionThe South China Sea (SCS) is the largestmarginal sea in the western Pacific (see Fig. 1). It con-nects with the SCS through the Taiwan Strait, with thePacific through the Luzon Strait, with the Sulu Seathrough the Mindoro and Balabac Straits and with theJava Sea and Andaman Sea through the Sunda Shelf(For convenience, here we refer to the section at 1.5°N,Fig. 2). It is shown that the seasonal SCS circulation ismostly affected by the summer/winter monsoon, andthe no…  相似文献   

溴素的有机化学──Ⅴ.溴代脂肪酸—2,3,5,6—四溴—1,4—苯二酯的合成     

崔伟  孙明昆  钱佐国  刘升一 《中国海洋大学学报(自然科学版)》1996,(3)

以1，4—苯二酚和不同的短链脂肪酸为基本原料，经溴代、加成、形成酰卤和酰化等反应，合成了六种新的溴化脂肪酸—2，3，5，6—四溴—1，4—苯二酯。通过对产物的碳、氢、溴等元素的定量分析，以及红外吸收光谱和氢核磁共振谱的研究，验证了化合物的结构。  相似文献   

中国沿海星虫动物门名录   总被引：2，自引：0，他引：2  

李凤鲁  周红  王玮 《中国海洋大学学报(自然科学版)》1992,(2)

取1980～1989年间在我国沿海进行星虫动物区系调查的标本和有关资料做了整理、鉴定,共39种,分隶于2纲,4目,6科,12属。其中1种在我国为首次记录。  相似文献   

Simulations of Annual Cycle of Phytoplankton Production and the Utilization of Nitrogen in the Yellow Sea     

Tian?TianEmail author  Hao?Wei  Jian?Su  Changsoo?Chung 《Journal of Oceanography》2005,61(2):343-357

A nutrient dynamic model coupled with a 3D physical model has been developed to study the annual cycle of phytoplankton production in the Yellow Sea. The biological model involves interactions between inorganic nitrogen (nitrate and ammonium), phosphate and phytoplankton biomass. The model successfully reproduces the main features of phytoplankton-nutrient variation and dynamics of production. 1. The well-mixed coastal water is characterized by high primary production, as well as high new production. 2. In summer, the convergence of tidal front is an important hydrodynamic process, which contributes to high biomass at frontal areas. 3. The evolution of phytoplankton blooms and thermocline in the central region demonstrate that mixing is a dominant factor to the production in the Yellow Sea. In this simulation, nitrate- and ammonium-based productions are estimated regionally and temporally. The northern Yellow Sea is one of the highly ranked regions in the Yellow Sea for the capability of fixing carbon and nitrogen. The annual averaged f-ratio of 0.37 indicates that regenerated production prevails over the Yellow Sea. The result also shows that phosphate is the major nutrient, limiting phytoplankton growth throughout the year and it can be an indicator to predict the bloom magnitude. Finally, the relative roles of external nutrient sources have been evaluated, and benthic fluxes might play a significant role in compensating 54.6% of new nitrogen for new production consumption.  相似文献   

海洋环流模式中不同近似假设下的海表高度   总被引：1，自引：1，他引：1  

李薇  张学洪  金向泽 《海洋科学进展》2003,21(2):132-141

Boussinesq近似是现代海洋环流模式中经常采用的假设，但随着海洋模式的不断发展完善以及气候研究应用的需要，有必要估算Boussinesq近似造成的模式误差。分别利用一个非Boussinesq近似的海洋模式与另一个结构相同且采用Boussinesq近似的模式计算海表高度，并同时利用模式预报的温度、盐度资料计算了比容异常高度。分析结果显示，这3种不同定义的海表高度无论空间结构，还是时间演变，都基本类似，尤其在热带海区最接近，差值≤1cm。Boussinesq近似意味着在模式中以体积守恒代替质量守恒，通常的做法是对其进行简单的质量补偿来保持质量守恒。比较说明，以质量补偿方法进行的高度订正对减小Boussinesq近似带来的误差没有本质的意义。  相似文献   

舟山沿海单齿螺（Monodonta labio）种群的年龄结构,生物量与生长特征     

王一农  王旭华 《海洋湖沼通报》1995,(1):54-60

本文报导了采自舟山沿海单齿螺种群的年龄结构、生物量与生长。自然种群的单齿螺年龄组成以１＾＋和２＾＋龄占优势，壳高、壳宽、壳口高、厣径、体重等生长参数的回归关系良好，单齿螺的生长完全可以用壳高来表示。单齿螺以第一、二年生长最快。采捕季节可定在四、五月间，采捕规格应在壳高１６ｍｍ以上。  相似文献