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为了揭示星斑川鲽(Platichthys stellatus)♀×大菱鲆(Scophthalmus maximus)♂杂交后代的种质遗传属性,作者采用线粒体(mt)DNA COI基因片段和控制区(D-loop)序列对星斑川鲽、大菱鲆及两种间的杂交后代(星斑川鲽♀×大菱鲆♂)遗传特性进行研究。研究结果显示,基于mtDNA COI和D-loop序列结果显示星斑川鲽和大菱鲆的种间遗传距离分别为22.9%和41.2%,相似的研究结果显示星斑川鲽♀×大菱鲆♂杂交后代与大菱鲆的遗传距离分别为22.9%和41.2%,遗传差异极其显著。而基于mtDNA D-loop序列结果显示星斑川鲽♀×大菱鲆♂杂交后代与星斑川鲽的遗传距离仅为0.4%,并且在mtDNA COI基因片段上两者序列完全一致,杂交后代在线粒体DNA上呈现明显的偏母遗传。 相似文献
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针对缺乏大比例尺地形图和历史灾害资料记录的地区,提出一种沟谷型泥石流淤积范围划分新方法。方法假定峰值流量时泥石流形成最大淤积范围,泥石流纵向淤积远端为堆积扇前缘。泥石流沟口过流断面最大平均流深(h0)被假定为堆积扇上其他过流断面的特定流深,h_0由峰值流量和沟口断面参数估算得到。利用堆积扇地形图,在相邻两条等高线间获得由h0确定的泥石流特征过流断面的位置和宽度,并对弯道处的特征过流断面宽度进行弯道超高修正。根据堆积扇上特征过流断面的分布确定泥石流淤积范围。3条泥石流案例用于方法验证,结果表明方法划定的淤积范围与实际淤积范围较一致,堆积扇上房屋的阻挡和局部相对平坦的地形是误差产生的主要原因。该方法具有操作简单、快速、客观、可重复的特点。 相似文献
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目前行人航迹推算逐渐成为室内定位研究与应用的热点。针对利用陀螺仪推算行人航向时存在较大累积误差的问题,本文提出了一种基于智能手机传感器的行人航向解算算法。该算法根据陀螺仪输出的角速度数据与手机传感器参数计算合适的阈值,实时调节PI调节器的误差补偿系数,对预处理后加速度计和磁力计数据解算的航向角进行补偿,并与陀螺仪数据互补滤波融合,得到融合后的航向角。试验基于低成本智能手机,分别在磁场强弱环境下采集手机传感器数据,对比分析本文算法与传统互补滤波算法及九轴数据融合算法在推算行人航向时的精度。试验结果表明,在室内磁干扰较强的环境下,本文算法与传统互补滤波算法、九轴数据融合算法相比定位精度分别提升了68.4%和65.9%,平均航向误差分别减小了3.4°和1.8°,验证了本文算法有较好的抗磁干扰性能,提高了行人航向角解算的可靠性。 相似文献
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鞍带石斑鱼仔稚幼鱼骨骼发育与生长特性研究 总被引：1，自引：0，他引：1
骨骼发育对于养殖鱼类苗种培育至关重要,骨骼畸形会影响仔稚幼鱼的外部形态及功能需求。作者利用骨骼染色技术和显微测量的方法对鞍带石斑鱼(Epinepheluslanceolatus)仔稚幼鱼的骨骼发育及生长特性进行连续观察和测量。实验结果表明:鞍带石斑鱼初孵仔鱼不存在骨与软骨结构,鞍带石斑鱼在变态前期和变态早期(长棘期)仔鱼的全长、颅骨长度、脊柱长度及尾鳍复合物的长度增加缓慢,骨骼发育过程中与摄食、呼吸及游泳功能相关的骨骼优先发育,如颅骨(颌骨、鳃)和鳍元件,且骨化顺序与发育顺序基本一致。进入变态晚期(收棘期),营养摄取及利用的器官发育趋于完善,稚鱼进入快速生长阶段。此外,在仔稚鱼发育早期,颅骨、脊柱以及尾鳍复合物中存在异速生长特性,呈现为在变态前期和变态早期(长棘期)颅骨和尾鳍复合物为正异速生长,而脊柱为负异速生长;在变态晚期(收棘期)和变态后期,颅骨为负异速生长,而脊柱和尾鳍复合物为近等速生长。因此,鞍带石斑鱼仔稚幼鱼的颅骨、脊柱及尾鳍复合物骨骼发育的异速生长现象与摄食、呼吸及游泳功能相适应。 相似文献
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Turbots( Schophthalmus maximus), one of the most important economic marine flatfish species, fail to undergo final spawning and spermiation naturally under artificial farming conditions. In vertebrates, reproduction is regulated by the brain-pituitary-gonadal axis(BPG-axis), and gonadotropin releasing hormone(GnRH) is one of its key components. Therefore, to better understand the physiology of reproduction in the turbot, three of the genes encoding GnRH subtypes— sbGnRH, c GnRH-II and sGnRH —were cloned and sequenced by isolating the cDNA sequences. The localizations and patterns of expression of their mRNAs were also evaluated during seasonal gonadal development. All three mRNAs were expressed abundantly in the brain; sbGnRH and sGnRH mRNAs were also detected in the gonads and pituitary gland, and sbGnRH expression was much higher than that of sGnRH, indicating the critical role of sbGnRH in regulating the BPG-axis. Moreover, the brain expression patterns of sbGnRH and sGnRH mRNAs showed an increased trend during gonadal development, peaking in mature stages. This indicated the direct regulation of gonadal development by the GnRH system. In addition, c GnRH-II mRNA expression showed no significant variations, suggesting that c GnRH-II is not critically involved in the control of reproduction. Further, the mRNA abundances of the three GnRH forms in the breeding season were significantly higher than those in immature and post-breeding stages in all analyzed brain areas. Therefore, we propose that sbGnRH is the most important hormone for the regulation of reproduction in turbot via the BPG-axis. These results will help in better understanding the reproductive endocrine mechanisms of turbots and lay the groundwork for additional studies aimed at comparing the reproductive physiology of wild individuals with those raised under artificial conditions. 相似文献
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日前,为增强干部职工的廉洁自律意识,进一步转变工作作风,诸城市国土资源局重点抓好“三个结合”,即：与强化学习教育相结合、与转变工作作风相结合、与落实监督检查相结合。不断加强廉政建设,收到了良好成效。工作中,该局组织党员干部参观了诸城市预防职务犯罪警示教育基地,进一步加强对干部理想信念和廉洁从政教育。严格落实《党风廉政建设责任制》、《工作人员行为准则》等规章制度,要求工作人员在办事过程中积极为群众提供优质、高效服务,切实为群众办实事、解难题。加强监督检查。在加强办理业务全程监督的基础上,突出抓好责任分解、检查考核、责任追究等关键环节,将廉政责任制进一步细化、量化,把工作表现、服务态度等也列入监督范围,让每个工作人员做到肩上有担子、有压力,工作有目标,行为有约束。通过以上措施,全体干部职工精神面貌焕然一新,说文明话、办文明事、做文明人在全局蔚然成风。 相似文献
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High temperature influences the homeostasis of fish.We investigated the effects of elevated temperature on tissues of Japanese flounder {Paralichthys olivaceus) by analyzing the histology and heat shock protein 70(hsp70) expression of fish reared in warm conditions.In this study,temperature was increased at l±0.5℃/day starting at 24±0.5℃,and was kept at that temperature for 5 days before the next rise.After raising temperature at the rate up to 32±0.5℃,tissue samples from midgut,spleen,stomach,liver,muscle,gill,heart,trunk kidney and brain were collected for histological analysis and mRNA assay.Almost all the tissues showed changes in morphological structure and hsp70 level at 32±0.5℃.Histological assessment of the tissues indicated that the gill had the most serious damage,including highly severe epithelial lifting and edema,curved tips and hyperemia at the ending of the lamellars,desquamation and necrosis.The next most severe damage was found in liver and kidney.The hsp70 levels in all the tissues first increased and then decreased.The gut,stomach,muscle,heart,and brain had the highest expressions in 6 h,whereas the spleen,liver,gill and kidney had the highest expressions in 2 h.Therefore,tissues with the most significant lesions(especially gill and liver) responded much earlier(2 h) in hsp70 expression than other tissues,and these tissues demonstrated the most marked histological disruption and elevated mRNA levels,making them ideal candidates for further studies on the thermal physiology of this species. 相似文献
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大菱鲆精子运动特征与精液pH的相关性研究 总被引：1，自引：0，他引：1
为探究大菱鲆(Scophthalmus maximus)精液质量与pH的关系及甘油对大菱鲆精子激活的影响,本研究通过计算机辅助精子分析系统分析检测了威海和烟台4家大菱鲆育苗场(A、B、C、D)的精子质量。研究发现,不同育苗场的大菱鲆精子活力存在显著的差异,A2A1DCB,精子预测寿命分别为11.7、7.1、6.8、4.6、3.3 min,所取精子的质量的存在显著差异。研究结果发现,大菱鲆精子运动特性与精子活化率相关性显著,其中精子活化率与平均曲线运动速度、平均直线运动速度、平均路径速度、精子头侧摆幅度、精子平均鞭打频率呈显著正相关(P0.05),与运动直线性、运动摆动性、运动前向性等参数无显著相关性;大菱鲆精子运动特性受pH影响显著,精液pH与平均曲线运动速度、平均直线运动速度、平均路径速度、精子头侧摆幅度、精子平均鞭打频率呈显著正相关(P0.05),而与运动直线性、运动摆动性、运动前向性等参数无显著相关性。同时还发现,精子活化率高、寿命长的大菱鲆精液pH偏弱碱性(pH=8.0),即大菱鲆精子适宜在弱碱性环境中活动。除此之外,向激活海水中加入梯度甘油(0.1、1、10、100、500、1 000 mmol/L),发现可以显著提高大菱鲆精子寿命,其中甘油浓度为100 mmol/L时,精子寿命延长至15.17 min±0.29 min,推测甘油在精子激活后可能被精子吸收并氧化分解提供能量。 相似文献