Molecular identification of scallop planktonic larvae using species-specific microsatellites   总被引：3，自引：0，他引：3  

ZHAN Aibin  HU Xiaoli  BAO Lisui  LU Wei  PENG Wei  WANG Mingling  HU Jingjie 《海洋学报(英文版)》2008,27(5):134-146

The identification of scallop larvae is essential to understand the population structure and community dynamics and to assess the potential environmental impacts caused by scallop larvae released or escaped. However, the larvae identification by morphological characteristics is notoriously difficult, mainly due to the small size （usually being less than 150 μm） and vague morphological characteristics among different scallop species. A simple and accurate molecular method was developed to identify four economically farmed scallop species, the Zhikong scallop Chlamys farreri, the noble scallop C. nobilis, the bay scallop Argopecten irradians and the Yesso scallop Mizuhopecten yessoensis. The tests used the high degree of species-specific microsatellite markers, which was specified by transferability analyses, assessed by reference individuals and evaluated by BLAST searches. The sensitivity test indicated that the species-specific microsatellites were sensitive enough for the detection of 1% -2% larvae in mixed plankton samples. Larvae collected from scallop hatcheries and their effluents and from the artificially controlled crosses were well identified to the species/hybrid level. The results demonstrated that the one-step PCR-based assay was technically simple, inexpensive and robust in identification analyses, and also less sensitive to initial quality of template DNA extracted from the ethanol-preserved samples for several years.  相似文献   

一种改进的图像边缘检测算法   总被引：2，自引：0，他引：2  

崔建军  詹世富  郑雄伟  顾俊凯 《地球科学与环境学报》2008,30(3)

对传统的微分边缘检测算法的缺陷进行分析和评价,并针对微分边缘检测算法的缺陷,提出了一种改进的边缘检测算法。新算法摈弃了传统边缘检测算法中将每个像素单独进行考虑的模式,而是将待检测像素及与其相邻的像素作为一个整体来考虑,通过将待检测像素为中心的某个领域进行整体计算,得出能够反映这个领域性质的某些参数,并以此作为边缘检测的判据,进行检测。此算法充分考虑到了中心像素与周围像素之间的关系,从而增加了梯度运算的可靠性。实验表明：新算法能够检测到更细的边缘,并且在椒盐噪声的情况下要优于常用的微分边缘检测算法。  相似文献   

论图书馆效益     

詹勋武 《成都信息工程学院学报》2000,15(3):270-274

社会主义市场经济要求图书馆加入经济大循环。从图书馆效益的现状及原因、市场经济对图书馆的要求及提高图书馆效益的有效措施 3个方面论述了图书馆效益  相似文献   

Detecting and attributing vegetation changes in Taihang Mountain,China   总被引：1，自引：0，他引：1  

HU Shi  WANG Fei-yu  ZHAN Che-sheng  ZHAO Ru-xin  MO Xiong-guo  LIU Liang-mei-zi 《山地科学学报》2019,(2)

Attributing vegetation changes provide fundamental information for ecosystem management,especially in mountainous areas which has vulnerable ecosystems. Based on the Normalized Difference Vegetation Index(NDVI) data, the spatial-temporal change of vegetation was detected in Taihang Mountain(THM) from 2000 to 2014. The topographical factors were introduced to interpret the response of vegetation variation to climate change and human activities. Results showed that the avegaged NDVI during growing season showed a single-peak curve distribution, with the largest value(0.628) among 1600-1800 m. A significant greening trend was detected in THM, with the largest increasing rate(0.0078 yr~(-1)) among the elevation of1600-1800 m and slope gradient between 3~5°. The partial correlation and multiple correlation analyses indicated that vegetation variation in more than81.8% pixels of the THM was mainly impacted by human activities. In the low elevation zones less than1000 m, increasing precipitation is the principle factor promoting vegetation restoration, whereas in the high elevation zones of THM, temperature is the restricted factors impacting vegetation variation.Considering the dramatic climate change in the future,further studies should be conducted to explore inherent mechanism of vegetation growth to dynamic environment changes.  相似文献   

漠阳江入海口海岸线分维及其机制分析     

李爽  詹文欢  姚衍桃 《海洋通报》2019,38(2):210-216

分维数是反映海岸线复杂程度的客观依据。结合国内外海岸线分形研究进展,本文以漠阳江入海口附近海域为研究区,基于Landsat 8 OLI遥感影像对研究区域提取瞬时水边线,并利用潮汐数据进行校正得到海岸线;利用Fractal Fox2.0计算获得该海岸线的分维数为1.071;通过对研究区的分形机制分析,认为漠阳江附近的断裂构造对海岸起宏观控制作用,从而对海岸线的分维值也起了决定作用。根据断裂体系模型计算得到西侧海岸线的理论分维值为1.089,而对数螺线模型计算得到东侧海岸线的分维值为1.171;实际分维值相对理论值偏小,说明海岸带的沉积与侵蚀影响了分维值。此外,根据本区海岸侵蚀原因的分析,对分维机制进行了探究,初步论证的分维值大小可为海岸的冲淤变化研究提供参考。  相似文献   

Relationships between body weight and other morphological traits in young sea cucumbers Apostichopus japonicas     

ZHAN Yaoyao  ZHANG Weijie  GE Chen  LIN Kai  LI Guang  SONG Jian  CHANG Yaqing 《海洋湖沼学报（英文）》2019,(2):759-766

Correlation, multiple regression, and path analyses were used to investigate the relationships between body weight and three other morphological traits in juvenile Japanese sea cucumbers Apostichopus japonicus . We measured live body weight (BW), body length (BL), numbers of papillae (NP), and numbers of tube feet (NF) at 60, 80, 100, and 130 days post-hatching (dph). We calculated path correlation coefficients, correlation indices ( R 2 ), and coefficients of determination with BW as the dependent variable and the other morphological traits as independent variables. The coefficient of variation for BW was high across all age groups, and all measured morphological traits were significantly correlated ( P < 0.01). BL had the greatest direct eff ect on BW across all age groups (60 dph, 0.526;80 dph, 0.404;100 dph, 0.620;and 130 dph, 0.681), while NF had the greatest indirect eff ect on BW across all age groups (60 dph, 0.528;80 dph, 0.452;100 dph, 0.666;and 130 dph, 0.603). Regression analyses between morphological traits and BW indicated that R 2 was greater than 0.85 only in the 100-dph specimens. The indirect eff ects of the other measured morphological traits on BW were age-dependent. The optimal regression equations, as determined with stepwise regression, were, for 60-dph specimens: BW 60 =10 (-3.04+0.092BL+0.014NP+0.014NF)( R 2 =0.632);for 80-dph specimens: BW 80 =10 (- 3.035+0.056BL+0.017NP+0.02NF)( R 2 =0.686);for 100-dph specimens: BW 100 =10 (- 3.742+0.069BL+0. 633 · lg (NP)+0. 464 · lg (NF))( R 2 =0.893);and for 130-dph specimens: BW 130 =10 (- 2.472+0.065BL+0.012NP)( R 2 =0.774). Our work clarified the correlation between various morphological traits and body weight of a commercially-important sea cucumber species ( A . japonicus ). Our predictive models for body weight might be useful for the aquaculture and selective breeding of A . japonicus . These models might also provide theoretical support for the indirect selection of traits that are difficult to select directly.  相似文献   

近景摄影测量支持下的地铁盾构管片姿态测量方法及其应用     

徐洲洋  肖东升 《测绘通报》2019,(1):75-78,143

传统的地铁盾构管片姿态测量的方法存在作业时间长,与施工流水线交叉作业劳动量大,且无法实时测量等不足。本文提出了利用近景摄影测量的方法对盾构管片姿态进行测量,通过在盾构管片环上铺设人工标志点,使用数码相机进行拍摄,利用光束法平常对摄影图片进行数据解算,得到解算标志点坐标后,再根据空间三点定圆心原理确定盾构环圆心点的坐标。试验结果表明,对比传统测量方法,新方法误差不超过3 cm,能够完全满足盾构管片姿态的测量精度要求,具有很好的推广价值。  相似文献   

无机盐改性对沸石覆盖技术控制底泥氮磷释放的影响研究   总被引：5，自引：0，他引：5  

林建伟  朱志良  赵建夫  詹艳慧 《湖泊科学》2007,19(1):52-57

通过试验,考察了无机盐改性对沸石覆盖控制底泥氮磷释放效果的影响,并对沸石覆盖技术控制底泥氮磷释放的机理进行初步探讨,结果表明:①NaCl及CaCl2改性对沸石吸附氨氮的性能影响不大;CaCl2改性可以提高沸石Ca2 的交换量而降低Na 的交换量,NaCl改性则可以明显降低Ca2 的交换量和增加Na 的交换量.②NaCl及CaCl2改性对沸石覆盖技术控制底泥氨氮的释放影响不大,而对控制底泥磷的释放则影响较大,沸石覆盖控制底泥磷释放效率从大到小依次为CaCl2改性沸石＞天然沸石＞NaCl改性沸石.③沸石覆盖技术控制底泥氨氮释放的机理为沸石的物理吸附和阳离子交换作用,控制底泥磷释放的机理包括沸石的机械阻挡作用和沸石与铵所交换出来的Ca2 对磷酸盐的固定作用.  相似文献   

川西甲基卡烧碳沟地区伟晶岩脉地球化学特征     

武文辉  赵春  詹涵钰  秦宇龙  李名则  徐云峰 《中国地质调查》2021,8(1):71-79

通过对烧碳沟地区伟晶岩的岩石学特征及地球化学特征进行分析,探讨了其成矿构造背景及物质来源等成矿地质条件.结果表明:烧碳沟一带伟晶岩的SiO2含量为73.46％～75.96％,全碱含量为4.71％～7.36％,里特曼指数为0.70～1.84,反映了伟晶岩的钙碱性特征;铝饱和指数A/CNK 为1.54～2.49,全部大于1...  相似文献   

陆表自然和人文要素相互作用——“全球变化及应对”重点专项研究进展          下载免费PDF全文

王开存  王灿  李龙辉  汪涛  吴国灿  付永硕  马倩  张井勇  蔡闻佳  曹静  喻朝庆  朱华晟  南卓铜  陈旻  张晶  纪多颖  沈妙根  唐文君  何斌  占文凤 《大气科学学报》2024,47(1):1-22

在国家重点研发计划支持下,项目提出了陆表不均一性检测和订正的新方法,解决了渐变型不均一性检测和订正的难题,构建了中国地表太阳辐射、气温、地温、风速和降水等参数均一化站点和格点数据集,修订了关于中国地表风速变化趋势、增温格局及其形成机制的结论。融合多源数据,构建并验证了千米级、流域级或县域级的电厂、人口、生物质能、取水量、氮排放、二氧化碳排放等影响自然系统的关键人文要素历史和未来预估数据集。构建了未来关键人文要素情景,研制了碳中和目标下甲烷和氧化亚氮排放情景和用于驱动全球模式的未来情景,预估了中国碳中和战略的实施对全球变暖的减缓作用,发现中国碳中和对远期和中期全球变暖的减缓作用显著。给出了中国各省份水体氮排放安全阈值及超越时间,阐明了中国粮食产量与氮施肥的关系,提出了在保障粮食安全的前提下减少水体氮排放的有效途径,指出重构城乡养分循环体系是同时保障粮食安全和恢复水质的必要途径。发现全球饱和水汽压差的年际变化与大气二氧化碳浓度上升速率的年际变化显著相关,阐明了饱和水汽压差变化在调控生态系统生产力中的重要角色以及多因素耦合作用在生态系统生产力变化中的复杂影响。建议更全面细致地评估中国各种碳中...  相似文献