Spatiotemporal dynamics of the climatic impacts on greenup date in the Tibetan Plateau     

Qing Chang  Jiahua Zhang  Wenzhe Jiao  Fengmei Yao  Siyuan Wang 《Environmental Earth Sciences》2016,75(20):1343

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Establishment and characterization of a testicular Sertoli cell line from olive flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>     

彭丽敏  郑媛  尤锋  吴志昊  邹玉霞  张培军 《中国海洋湖沼学报》2016,34(5):1054-1063

The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.  相似文献   

Tracing submarine groundwater discharge flux in Tolo Harbor,Hong Kong (China)     

Yi Liu  Jiu Jimmy Jiao  Ho Kwan Cheng 《Hydrogeology Journal》2018,26(6):1857-1873

Submarine groundwater discharge (SGD) is an important pathway for groundwater and associated chemicals to discharge to the sea. Groundwater levels monitored along a transect perpendicular to the shoreline are used to calculate SGD flux from the nearshore aquifer to Tolo Harbor, Hong Kong (China). The calculated SGD flux—recharge/discharge measured with Darcy’s Law methods—agrees well with estimates based on geo-tracer techniques and seepage meter in Tolo Harbor during previous studies. The estimated freshwater SGD is 1.69–2.0 m²/d at the study site and 0.3?±?0.04 cm/d for the whole of Tolo Harbor, which is comparable to the river discharge (0.25?±?0.07 cm/d) and precipitation (0.45?±?0.15 cm/d). The tide-driven SGD in the intertidal zone is 13.98–17.59 m²/d at the study site and 2.42?±?0.56 cm/d for the whole of Tolo Harbor. The SGD occurring in the subtidal zone and the bottom of Tolo Harbor is 3.12?±?4.63 cm/d. Fresh SGD accounts for ~5% of the total SGD, while the rest (~95%) is contributed by saline SGD driven by various forces. About 96% of the tide-driven SGD in the intertidal zone occurs in the ebbing tide period because the head difference between the groundwater level and sea level is great during this period. Tide-driven SGD in the spring tide is ~1.2 times that during neap tide. The tidal fluctuation amplitude and tide-driven SGD in the intertidal zone are positively correlated to each other; thus, a spring neap variation of the tide-driven SGD is observed.  相似文献   

A comparative analysis of the NDVIg and NDVI3g in monitoring vegetation phenology changes in the Northern Hemisphere     

Qing Chang  Wenzhe Jiao  Fengmei Yao 《国际地球制图》2018,33(1):1-20

Phenology is a sensitive and critical feature of vegetation and is a good indicator for climate change studies. The global inventory modelling and mapping studies (GIMMS) normalized difference vegetation index (NDVI) has been the most widely used data source for monitoring of the vegetation dynamics over large geographical areas in the past two decades. With the release of the third version of the NDVI (GIMMS NDVI3g) recently, it is important to compare the NDVI3g data with those of the previous version (NDVIg) to link existing studies with future applications of the NDVI3g in monitoring vegetation phenology. In this study, the three most popular satellite start of vegetation growing season (SOS) extraction methods were used, and the differences between SOSg and SOS3g arising from the methods were explored. The amplitude and the peak values of the NDVI3g are higher than those of the NDVIg curve, which indicated that the SOS derived from the NDVIg (SOSg) was significantly later than that derived from the NDVI3g (SOS3g) based on all the methods, for the whole northern hemisphere. In addition, SOSg and SOS3g both showed an advancing trend during 1982–2006, but that trend was more significant with SOSg than with SOS3g in the results from all three methods. In summary, the difference between SOSg and SOS3g (in the multi-year mean SOS, SOS change slope and the turning point in the time series) varied among the methods and was partly related to latitude. For the multi-year mean SOS, the difference increased with latitude intervals in the low latitudes (0–30°N) and decreased in the mid- and high-latitude intervals. The GIMMS NDVI3g data-sets seemed more sensitive than the GIMMS NDVIg in detecting information about the ground, and the SOS3g data were better correlated both with the in situ observations and the SOS derived from the Moderate Resolution Imaging Spectroradiometer NDVI. For the northern hemisphere, previous satellite measures (SOS derived from GIMMS NDVIg) may have overestimated the advancing trend of the SOS by an average of 0.032 d yr^–1.  相似文献   

如何用ArcGIS 9.3制作1∶10000地形图     

石礁 《测绘与空间地理信息》2018,(2):176-177

随着1∶10 000数据的更新,制图文件已不再是附属产品,并逐步成为1∶10 000地形图制作过程中的重要环节。本文简要介绍了用ArcGIS 9.3制图的过程和注意事项。  相似文献   

柴达木盆地一里坪盐湖富锂卤水特征、储层物性及富水区分析     

赵艳军  焦鹏程  汪明泉  张大义  朱振光  胡宇飞 《地质学报》2021,95(7):2062-2072

一里坪盐湖是柴达木盆地中一个典型的富锂卤水矿床,具有重大的经济价值.本文以查明盐湖深部卤水分布为目的,从卤水储层的空间分布特征和储层物性的角度对富水区进行预测,为卤水开发提供依据.研究区位于一里坪成盐盆地沉积中心区域,晚更新世末期以来,以沉积浅湖相的含淤泥和黏土粉砂岩,盐湖相的含粉砂和黏土的石盐岩为特征.在垂向上,由上下两套石盐岩夹中部粉砂岩组成一里坪富锂卤水的储层;平面上,研究区北部主要为浅湖相,往南过渡为盐湖相,石盐岩厚度在东南部最大.对储层厚度、孔隙度和给水度的分析表明,第Ⅱ矿层孔隙型和晶间型卤水优质储层均位于研究区东部,可作为卤水资源勘查开采的重点区域.  相似文献   

柴达木盆地一里坪盐湖成盐卤水成分变化及其意义——来自LA- ICP- MS分析石盐流体包裹体组成的证据   总被引：1，自引：0，他引：1  

胡宇飞  汪明泉  赵艳军  焦鹏程 《地质学报》2021,95(7):2109-2120

一里坪盐湖是柴达木盆地一个以富锂卤水为主的干盐湖,蕴藏有丰富的卤水钾锂资源,目前对成矿过程中卤水的成分及其演变规律方面的研究较少.论文选取一里坪盐湖中部钻孔HC2105岩芯的石盐为主要对象,利用激光剥蚀-电感耦合等离子质谱法开展了单个石盐原生流体包裹体成分分析,探讨恢复了一里坪盐湖地区晚更新世以来成盐期的古卤水成分.结果表明,一里坪盐湖下层石盐古卤水Li、B、K等离子浓度均高于上层石盐,这说明晚更新世成矿条件更好.古卤水中Li、B、K和Mg等离子具有正相关性,再结合对Ca离子浓度变化的研究,说明一里坪盐湖的主要物质来源为那棱郭勒河,深部卤水对其物质来源贡献较小;石盐流体包裹体中各离子浓度的演变规律与钻孔沉积韵律以及古气候变化三者基本可以拟合,从而证实盐湖卤水成分变化受到古气候变化的影响,干冷期可能更有利于富锂卤水矿的形成.  相似文献   

高寒冻土区生物结皮对土壤理化属性的影响   总被引：1，自引：0，他引：1  

明姣  盛煜  金会军  张泽  杜玉霞 《冰川冻土》2021,43(2):601-609

生物结皮是高寒地区地被层的重要组分之一。其作为地表特殊的结构层,能够改变地表结构及土壤理化属性,从而影响冻土环境。迄今为止,关于青藏高原高寒生态系统中生物结皮对土壤理化属性的影响尚不清楚。以青藏高原高寒冻土区生物结皮为研究对象,初步研究了生物结皮的特征及其对土壤理化属性的影响。结果表明：生物结皮在高寒草甸退化过程中广泛发育,主要以藻结皮为主,其盖度可达37.3%~51.7%,结皮层平均厚度为12.6 mm。由于生物结皮的发育,高寒地区5~20 cm土层粉粒含量有所增加,但差异不显著,而结皮层土壤田间持水量相比于裸地表层（2 cm）增加了10%~40%,结皮层容重较裸地降低了30%;两种类型藻结皮均显著增加了结皮层及其下0~20 cm土层土壤有机质,而深色藻结皮增加了结皮层及其下0~20 cm土层土壤全氮含量,浅色藻结皮仅增加了结皮层土壤全氮含量,对其下0~20 cm土层土壤全氮含量没有显著影响;生物结皮对土壤pH没有显著影响;生物结皮是高寒生态系统植被退化过程中的关键环节。研究结果为揭示生物结皮在高寒生态系统中发挥重要生态功能提供依据。  相似文献   

戈壁荒漠覆盖区地质调查中综合地球物理方法与实践          下载免费PDF全文

陈超  许顺芳  王国灿  胡正旺  刘江平  刘营  梁青  杜劲松  王林松  汪利民 《地球科学》2021,46(8):3028-3038

戈壁荒漠覆盖区地质填图是新时期地质调查工作的重点内容之一.覆盖区地质填图的基本目标包括两个方面,一是揭示覆盖层下伏基岩面地质结构,即填绘基岩面地质图;二是揭示覆盖层地质结构,包括地表覆盖层地质结构和覆盖层的三维地质结构.综合利用地球物理方法可以获得覆盖区地下地质结构、岩石属性、沉积物分布与层状结构等信息.而经济有效地选择和组合实施地球物理方法是完成覆盖区地质填图目标任务的重要保障.基于近年在新疆东天山地区开展的覆盖区地质填图工作,提出了针对多覆盖层结构背景下地质填图的综合地球物理方法组合的技术策略.综合地球物理方法以及新技术在巴里坤盆地和哈密盆地的应用展示了其效果,并为在戈壁荒漠覆盖区地质调查过程中如何开展地球物理工作提供了示范和借鉴.   相似文献   

东特提斯喜马拉雅下白垩统碎屑锆石U-Pb年代学及其古地理          下载免费PDF全文

焦显伟  石玉若  杨天水  边伟伟  王锁  彭文骁 《地球科学》2021,46(8):2850-2859

东特提斯喜马拉雅在中生代位于东冈瓦纳大陆的结合部位,其古地理对于了解东冈瓦纳大陆裂解至关重要.对东特提斯喜马拉雅塔嘎地区沉积地层进行了详细的碎屑锆石U-Pb年代学研究.结果表明,东特提斯喜马拉雅塔嘎地区采样剖面沉积下限为126.6±2.7 Ma.碎屑锆石年龄谱显示东特提斯喜马拉雅塔嘎地区采样地层主要包含~520 Ma、~890 Ma和~1 200 Ma的特征峰值年龄,对比结果表明东特提斯喜马拉雅塔嘎地区沉积地层碎屑锆石年龄谱与印度东部和澳大利亚西南部地层碎屑锆石年龄谱具有一定的相似性.结合东冈瓦纳岩浆活动记录以及该剖面下部玄武岩年龄,东特提斯喜马拉雅塔嘎地区地层沉积于东特提斯喜马拉雅从东冈瓦纳大陆分离时期,其物质来源可能为印度东部、澳大利亚西南部以及南极大陆.   相似文献