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An operational framework is developed to serve as a guideline for investigating causal relationships between environmental stressors and effects on marine biota. Because of the complexity and variability of many marine systems, multiple lines of evidence are needed to understand relationships between stressors and effects on marine resources. Within this framework, a weight of evidence approach based on multiple lines of evidence are developed and applied in a sequential manner by (1) characterizing the study system which involves determining if target biota are impaired, assessment of food and habitat availability, and measuring contaminant levels in the environment, (2) assessing direct effects of contaminant exposure on target biota using biomarkers and assessing indirect effects of exposure using suites of bioindicators, and (3) applying standard causal criteria based on epidemiological principles and diagnostic health profiling techniques to assess potential causes. Use of multiple lines of evidence should also reduce the risk of false positives (Type I error or falsely concluding that there is a causal relationship when there is none) and false negatives (Type II error or falsely concluding there is not a causal relationship when there actually is). Understanding causal relationships and the mechanistic processes between environmental stressors and effects on biota is important in the effective management and restoration of impaired marine ecosystems.  相似文献   
74.
We present the first statistical analysis of 27 Ultraviolet Optical Telescope (UVOT) optical/ultraviolet light curves of gamma-ray burst (GRB) afterglows. We have found, through analysis of the light curves in the observer's frame, that a significant fraction rise in the first 500 s after the GRB trigger, all light curves decay after 500 s, typically as a power law with a relatively narrow distribution of decay indices, and the brightest optical afterglows tend to decay the quickest. We find that the rise could be either produced physically by the start of the forward shock, when the jet begins to plough into the external medium, or geometrically where an off-axis observer sees a rising light curve as an increasing amount of emission enters the observers line of sight, which occurs as the jet slows. We find that at 99.8 per cent confidence, there is a correlation, in the observed frame, between the apparent magnitude of the light curves at 400 s and the rate of decay after 500 s. However, in the rest frame, a Spearman rank test shows only a weak correlation of low statistical significance between luminosity and decay rate. A correlation should be expected if the afterglows were produced by off-axis jets, suggesting that the jet is viewed from within the half-opening angle θ or within a core of a uniform energy density  θc  . We also produced logarithmic luminosity distributions for three rest-frame epochs. We find no evidence for bimodality in any of the distributions. Finally, we compare our sample of UVOT light curves with the X-ray Telescope (XRT) light-curve canonical model. The range in decay indices seen in UVOT light curves at any epoch is most similar to the range in decay of the shallow decay segment of the XRT canonical model. However, in the XRT canonical model, there is no indication of the rising behaviour observed in the UVOT light curves.  相似文献   
75.
Extractable biomarkers can help elucidate the environment and biota of ancient glaciations, although the method must be applied with care, as glacial sediments have a potential for incorporation of older detrital carbon. In Phanerozoic glacial sediments, the distinct elemental, molecular and isotopic compositions of the terrestrial and marine biomass allow discrimination between primary marine and redeposited terrestrial organic matter. However, as the Proterozoic biosphere was largely microbial and marine, biomarker and isotopic analyses are insufficient for distinguishing primary organic matter from secondary reworked organic matter. Here, we report the combined application of Raman spectroscopy and biomarker analysis to Precambrian glacial sediments, which, together, allows discrimination between mixed pools of organic carbon and provides a promising new approach for rapidly screening Precambrian sediments for immature organic matter amenable to biomarker analysis.  相似文献   
76.
Mass changes of Blue Glacier, USA are calculated from topographic maps made from vertical aerial photography in late summer of 1939, 1952, 1957, and 1987, along with laser altimetry flown in June 1996. Changes in elevation between maps were adjusted for seasonal variations in the snow cover, and to account for the ablation between the date of photography and 1 October. Topography obtained from the laser altimetry was adjusted for snow thickness and glacier motion to estimate topography of 1 October 1995. The mass of Blue Glacier has changed less than 7 m (water equivalent) during this 56 year period which is minor compared with other glaciers in the region and elsewhere in the world. Glacier-average annual mass balances, beginning in 1956, have been calculated either from stake measurements and probing of late-season snow, or from a regression analysis using late-season measurements of the equilibrium line altitude. A comparison with the changes derived from surface maps shows values obtained from field measurements are too positive by about 0.4 m a?1 , indicating that considerable caution is needed when interpreting time series of mass balance. Two alternative time series of mass balance consistent with the long-term mass changes are created by making simple adjustments: (1) a single constant is subtracted from each value so that the series is consistent with the 1957–95 mass change; (2) one constant is subtracted from each value over 1957–87 and another is subtracted from each value over 1987-95 so that the series is consistent with both the 1957–87 and 1987–95 mass changes. The mass balance of Blue Glacier was generally positive until the mid-1970s and negative since. The fluctuations of mass balance closely resemble those of snowfall on the glacier as estimated from the joint distribution of temperature and precipitation. The climate in western Washington was cooler and wetter during the decade before the mid-1970s, but the trend since has been towards warmer and drier conditions.  相似文献   
77.
This study investigated the possibility that changes in the surface morphology of mussel (Perna perna) gill filaments may be used to indicate the relative toxicity of pollutants in the marine environment. Healthy, adult P. perna were collected and immersed in 2 free-flow tanks. Mercury was added to seawater as it entered tank 1 to achieve a constant level of 50 μg/l−1 over 24 days. Uncontaminated seawater was circulated over the mussels in tank 2 (control) for the same period. A 25 mm2 area of gill filament was removed from each of the 5 specimens before and after 24 days immersion in tank 2, and after immersion for 1, 2, 4, 8, 16 and 24 days in tank 1. These were examined using a scanning electron microscope. The remaining soft tissues from each animal were analysed for mercury using an atomic absorption spectrometer. Mercury concentration increased from 0.13 (pre-immersion) to 87 μg/g after day 24. Surface morphology remained normal for all animals in tank 2 and for those exposed to Hg for up to 8 days. However, from 16 to 24 days exposure there was a gradual increase in the diameters of microvilli, a depletion of abfrontal cilia, an increase in abnormal, perhaps necrotic cells and an unusual increase in the number of cilia on the lateral surfaces. These results confirm that P. perna is an efficient bio-accumulator and suggest that their gill pathomorphology may be a useful indicator of toxicity.  相似文献   
78.
We have designed a new instrument to measure the current flowing along balloon rigging line during flights through thunderstorms. This instrument was tested in a high voltage facility and used to collect line current data during one balloon flight into a thunderstorm. Using these data, worst-case calculations are made; as such, we claim that they are the upper limits of any alteration (to the measured electric field or particle charge) that may occur, and the real number is likely much less. It is postulated the rigging-line current could have two separate effects on the measured electric field: (1) reduction of the field due to emission of corona ions, and (2) enhancement of the field due to the insertion of a long thin ‘conductor.' Even with current as high as 1 μA (the largest measured was around 50–100 nA), these two effects were found to be about −1% and +1%, respectively. Also, the calculated worst-case alteration to charged precipitation measurements is about 0.1 pC. Thus, with proper efforts to make the rigging line as poor a conductor as possible, it seems that we are justified in stating that these effects are negligible.  相似文献   
79.
Stratigraphic sections are often sampled at well-defined discrete points. Because of the incompleteness of the fossil record, a particular species may not be observed even when it is extant at a sampling point. We introduce a model and Bayesian analysis for estimating the true time of disappearance of a lineage from a section in the face of the possibility that failure to find the species beyond its observed stratigraphic range may represent false negatives. We incorporate proper prior information, including an estimated longevity of the species and the probability that it will be observed if extant. Our analysis produces a posterior density for the true extinction time of the species. Summaries of this probability distribution provide a point estimate of the extinction time, a standard deviation for the uncertainty in the estimate, and confidence intervals for the time of extinction. We apply our model to stratigraphic ranges of benthic foraminifera collected from the early Late Cretaceous (Cenomanian and Turonian) from Eastbourne, England.  相似文献   
80.
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