Cultivation Ages Effect on Soil Physicochemical Properties and Heavy Metal Accumulation in Greenhouse Soils   总被引：1，自引：0，他引：1  

Jun Wang  Wenkui Mi  Peipei Song  Hui Xie  Lusheng Zhu  Jinhua Wang 《中国地理科学(英文版)》2018,28(4):717-726

The intensive management practices in greenhouse production may alter the soil physicochemical properties and contribute to the accumulation of heavy metals (HMs). To determine the HM concentrations in vegetable soil in relation to soil physicochemical properties and cultivation age, we conducted a soil survey for typical greenhouse soils in Shouguang, China. The results indicated that Cd is a major HM pollutant in the tested soils, as the only HM element exceeding the allowed limit for vegetable soil. The surveyed data was analyzed with regression analysis, correlation analysis and canonical correspondence analysis (CCA). A positive correlation is observed between HM pollution level and cultivation age. CCA results suggest that the HM pollution level and distribution in soil are significantly affected by soil physicochemical properties, which was a function of years of cultivation as revealed by regression analysis. In summary, cultivation age is an important factor to affect soil physicochemical properties (organic matter and inorganic nutrients) as well as HM contamination.  相似文献   

Effect of decreasing temperature on the strobilation of Aurelia sp.1     

石岩  于志刚  甄毓  王国善  王勋功  米铁柱 《中国海洋湖沼学报》2018,36(2):465-472

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A thermostable serralysin inhibitor from marine bacterium Flavobacterium sp. YS-80-122     

梁朋娟  李尚勇  王昆  王芳  邢孟欣  郝建华  孙谧 《中国海洋湖沼学报》2018,36(2):483-489

Serralysin inhibitors have been proposed as potent drugs against many diseases and may help to prevent further development of antibiotic-resistant pathogenic bacteria. In this study, a novel serralysin inhibitor gene, l up I, was cloned from the marine bacterium F lavobacterium sp. YS-80-122 and expressed in Escherichia coli. The deduced serralysin inhibitor, Lup I, shows 40% amino acid identity to other reported serralysin inhibitors. Multiple sequence alignment and phylogenetic analysis of Lup I with other serralysin inhibitors indicated that Lup I was a novel type of serralysin inhibitor. The inhibitory constant for Lup I towards its target metalloprotease was 0.64 μmol/L. Lup I was thermostable at high temperature, in which 35.6%–90.7% of its inhibitory activity was recovered after treatment at 100°C for 1–60 min followed by incubation at 0°C. This novel inhibitor may represent a candidate drug for the treatment of serralysin-related infections.  相似文献   

一株光合细菌菌株RPD-1的生物学特性及其在菲律宾蛤仔育苗中的应用     

王芳  李筠  盛军  孙谧 《海洋科学》2010,34(7):1-7

RPD-1菌株分离自青岛栈桥近岸海水,根据其生理生化特征鉴定为紫色非硫菌科红假单胞菌属(Rhodopseudomonas)。菌株RPD-1适盐范围较广,在NaCl含量为0～8%的培养基中均可生长,在NaCl为3%的培养基中生长最快;在初始pH为6.0～9.0的培养基中均可生长,pH为7.5时生长最快;不同光源的光照条件下,生长速度不同,白炽灯光照下,生长速度快,日光灯照下,生长速度慢;RPD-1菌株蛋白质含量为58.5%,含各种必需氨基酸。菲律宾蛤仔(Ruditapes philippinarum)育苗实验结果表明,RPD-1菌株能提高菲律宾蛤仔幼虫的存活率和变态率;水体氨态氮测定结果表明,RPD-1菌株能显著降低养殖水体中的氨氮。  相似文献   

构造生烃理论及其在页岩气上的应用     

熊斌辉  米立军  王存武 《海洋地质译丛》2010,(4):1-6

生烃是地层有机质生成油气的化学平衡。由于油气的密度低于干酪根,它是典型的体积增大化学反应。与实验室开放系统不同,地层有机质生烃反应能等于活化能加排烃能。成熟阶段的地层比较致密,排烃能较大,与开放系统相比,形成了欠生烃。构造运动形成裂隙网,大大降低地层排烃能,使欠生烃的有机质短时间集中生烃,笔者称之为构造生烃。成熟地层通常较致密,排烃能较高,较多欠生烃有机质成为页岩气的物质基础。致密地层在过成熟条件下还有大量欠生烃有机质。经典生烃理论认为Ro大于2.0就基本不生烃,而许多Ro达到3.0,个别甚至4.0的页岩气发现,证明欠生烃的存在。分子越小,排烃能越低,相对致密地层生气的反应能通常最低,更多的有机质形成了页岩气,页岩气资源潜力巨大。页岩气的开采速度比地层自然排烃速度高出多个数量级,天然气排出最快的,最有利于生成天然气的化学平衡。勘探开发实践表明页岩含气量与其TOC成正比,笔者认为这正好预示着页岩中存在游离气、吸附气和有机质的化学平衡。游离气压降低,吸附气就会解吸附;吸附气解吸附,有机质就会生烃。有些过成熟岩石存在未—低成熟度Tmax值。这样低的Tmax值预示着,有机质能够在地层被压裂后随着排烃能的降低而满足反应条件,而成为潜在资源。页岩气的开采与普通气层相比更复杂、更漫长、更巨大。  相似文献   

时序InSAR监测京雄城际铁路河北段地面沉降     

葛鹏飞  刘辉  陈蜜  李昱  丁瑞力  刘菲 《测绘通报》2022,(7):64-70

本文以京雄城际铁路河北段固安站至雄安站沿线作为研究区,利用2018—2020年共34景Sentinel-1B影像,基于小基线集雷达干涉测量技术(SBAS-InSAR)获取京雄城际铁路河北段沿线的地面沉降时空分布信息,结合空间自相关分析方法,揭示研究区地面沉降的空间分布格局,并对沉降原因进行初步分析。研究结果表明,京雄城际铁路河北段沿线地面沉降发展由北向南存在一定的差异。北部年均沉降速率小于10 mm/a,南部沉降较为严重,最大年均沉降速率达-105.6 mm/a,且沿线西部年均沉降速率高于东部区域。通过分析影响因素得知,地面沉降量与地下水埋深值存在相关性,地下水埋深高的地区地面沉降量较高。同时结合研究区土地利用变化结果发现,城市化建设所产生的静载荷对京雄城际铁路沿线的地面沉降产生一定的影响。  相似文献   

Patterns of Gene Expression Variation across Body Parts of the Hydrothermal Vent Shrimp Nautilocaris saintlaurentae     

Kim  Ryeo-Ok  Won  Eun-Ji  Kang  Hyun Mi  Ju  Se-Jong  Kim  Se-Joo 《Ocean Science Journal》2019,54(4):595-609

Ocean Science Journal - Since deep-sea hydrothermal vents have been targeted for mining mineral resources, concerns about the potential impacts of mining on vent ecosystems have gradually...  相似文献   

Optimal precursors of double-gyre regime transitions with an adjoint-free method     

YUAN Shijin  LI Mi  WANG Qiang  ZHANG Kun  ZHANG Huazhen  MU Bin 《海洋湖沼学报（英文）》2019,(4):1137-1153

In this paper, we find the optimal precursors which can cause double-gyre regime transitions based on conditional nonlinear optimal perturbation (CNOP) method with Regional Ocean Modeling System (ROMS). Firstly, we simulate the multiple-equilibria regimes of double-gyre circulation under different viscosity coefficient and obtain the bifurcation diagram, then choose two equilibrium states (called jet-up state and jet-down state) as reference states respectively, propose Principal Component Analysis-based Simulated Annealing (PCASA) algorithm to solve CNOP-type initial perturbations which can induce double-gyre regime transitions between jet-up state and jet-down state. PCASA algorithm is an adjoint-free method which searches optimal solution randomly in the whole solution space. In addition, we investigate CNOP-type initial perturbations how to evolve with time. The results show:(1) the CNOP-type perturbations present a two-cell structure, and gradually evolves into a three-cell structure at predictive time;(2) by superimposing CNOP-type perturbations on the jet-up state and integrating ROMS, double-gyre circulation transfers from jet-up state to jet-down state, and vice versa, and random initial perturbations don't cause the transitions, which means CNOP-type perturbations are the optimal precursors of double-gyre regime transitions;(3) by analyzing the transition process of double-gyre regime transitions, we find that CNOP-type initial perturbations obtain energy from the background state through both barotropic and baroclinic instabilities, and barotropic instability contributes more significantly to the fast-growth of the perturbations. The optimal precursors and the dynamic mechanism of double-gyre regime transitions revealed in this paper have an important significance to enhance the predictability of double-gyre circulation.  相似文献   

CNOP-P-based parameter sensitivity for double-gyre variation in ROMS with simulated annealing algorithm     

Yuan  Shijin  Zhang  Huazhen  Li  Mi  Mu  Bin 《中国海洋湖沼学报》2019,37(3):957-967

Reducing the error of sensitive parameters by studying the parameters sensitivity can reduce the uncertainty of the model, while simulating double-gyre variation in Regional Ocean Modeling System(ROMS). Conditional Nonlinear Optimal Perturbation related to Parameter(CNOP-P) is an effective method of studying the parameters sensitivity, which represents a type of parameter error with maximum nonlinear development at the prediction time. Intelligent algorithms have been widely applied to solving Conditional Nonlinear Optimal Perturbation(CNOP). In the paper, we proposed an improved simulated annealing(SA) algorithm to solve CNOP-P to get the optimal parameters error,studied the sensitivity of the single parameter and the combination of multiple parameters and verified the effect of reducing the error of sensitive parameters on reducing the uncertainty of model simulation. Specifically, we firstly found the non-period oscillation of kinetic energy time series of double gyre variation, then extracted two transition periods, which are respectively from high energy to low energy and from low energy to high energy. For every transition period, three parameters, respectively wind amplitude(WD), viscosity coefficient(VC)and linear bottom drag coefficient(RDRG), were studied by CNOP-P solved with SA algorithm. Finally,for sensitive parameters, their effect on model simulation is verified. Experiments results showed that the sensitivity order is WDVCRDRG, the effect of the combination of multiple sensitive parameters is greater than that of single parameter superposition and the reduction of error of sensitive parameters can effectively reduce model prediction error which confirmed the importance of sensitive parameters analysis.  相似文献   

牡蛎软体中抗菌蛋白的制备及活性研究   总被引：1，自引：0，他引：1  

王春波  兰晓明  王洪江  牟茜  孙谧 《海洋与湖沼》2010,41(1):33-38

采用超滤法、聚丙烯酰胺凝胶电泳法、CM-Sepharose FF阳离子交换柱层析法等对牡蛎软体中抗菌蛋白(Antibacterial protein,AP)进行分离纯化,并采用体外实验观察其对常见致病菌的抗菌作用。结果表明,此蛋白分子量在16.4—39kDa,蛋白质含量≥70%。抗菌蛋白对生孢梭菌、白色念珠菌、变形杆菌和金黄色葡萄球菌、表皮葡萄球菌、大肠埃希氏菌、铜绿假单胞菌、肺炎克雷伯杆菌、肠球菌、不动菌属均有较强的抗菌作用,MIC范围在0.125—32.0μg/ml之间,MBC范围在0.250—1.00mg/ml之间。以透射电镜观察AP对大肠杆菌的作用,提示AP可能通过作用于菌体细胞壁,发挥其抗菌作用。  相似文献