|
|||||
|
|
| 牡蛎软体中抗菌蛋白的制备及活性研究 | |
| 引用本文： | 王春波,兰晓明,王洪江,牟 茜,孙 谧.牡蛎软体中抗菌蛋白的制备及活性研究[J].海洋与湖沼,2010,41(1):33-38. |
| 作者姓名： | 王春波 兰晓明 王洪江 牟 茜 孙 谧 |
| 作者单位： | 1. 青岛大学医学院青岛,266012 2. 青岛大学医学院青岛,266012;青岛卫生学校青岛,266071 3. 中国水产科学研究院黄海水产研究所,青岛,266071 |
| 基金项目： | 国家863计划资助项目,2003AA625070号;;山东省科技厅资助项目,22130107号 |
| 摘 要： | 采用超滤法、聚丙烯酰胺凝胶电泳法、CM-Sepharose FF阳离子交换柱层析法等对牡蛎软体中抗菌蛋白(Antibacterial protein,AP)进行分离纯化,并采用体外实验观察其对常见致病菌的抗菌作用。结果表明,此蛋白分子量在16.4—39kDa,蛋白质含量≥70%。抗菌蛋白对生孢梭菌、白色念珠菌、变形杆菌和金黄色葡萄球菌、表皮葡萄球菌、大肠埃希氏菌、铜绿假单胞菌、肺炎克雷伯杆菌、肠球菌、不动菌属均有较强的抗菌作用,MIC范围在0.125—32.0μg/ml之间,MBC范围在0.250—1.00mg/ml之间。以透射电镜观察AP对大肠杆菌的作用,提示AP可能通过作用于菌体细胞壁,发挥其抗菌作用。 |
| 关 键 词： | 牡蛎软体 蛋白 制备 抗菌作用 |
| 收稿时间： | 2008/12/15 0:00:00 |
| 修稿时间： | 2009/10/10 0:00:00 |
PREPARATION AND ANTIBACTERIAL ACTIVITY OF OYSTER PROTEIN |
|
| WANG Chun-Bo,LAN Xiao-Ming,WANG Hong-Jiang,MU Qian and SUN Mi.PREPARATION AND ANTIBACTERIAL ACTIVITY OF OYSTER PROTEIN[J].Oceanologia Et Limnologia Sinica,2010,41(1):33-38. | |
| Authors: | WANG Chun-Bo LAN Xiao-Ming WANG Hong-Jiang MU Qian and SUN Mi |
| Institution: | 1. Qingdao University Medical College;Qingdao;266012;2. Qingdao Health School;266071;3. Yellow Sea Fisheries Research Institude Chinese Academy of Fishery Sciences;266071 |
| Abstract: | Antibacterial protein (AP) was prepared and purified by means of ultrafiltration technology and CM- Sepharoe FF positive ion column chromatography from oyster muscle. The in vitro activity of AP was also studied. The AP demonstrated in vitro activity against test strains, including Clostridium sporogenes, Candida albicans, Bacillus proteus, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterococcus, and Acinetobacter. The MIC range and th... |
| Keywords: | Muscle of oyster Protein Preparation Antibacterial activity |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《海洋与湖沼》浏览原始摘要信息 | |
| 点击此处可从《海洋与湖沼》下载免费的PDF全文 | |