Survey of cyanomyovirus abundance in Shantou coastal waters by g20     

李传标  丁俊  周立镇  张昭  李升康  刘文华  温小波 《中国海洋湖沼学报》2015,33(3):604-615

To understand the genetic diversity and population changes in cyanophages in the coastal waters of Shantou,northeast South China Sea,we used the capsid assembly protein gene g 20 as a marker of the abundance and phylogeny of natural cyanomyovirus communities. The abundance of total viruses,heterotrophic bacteria,and picophytoplankton in the coastal waters was monitored with flow cytometry. Hydrological parameters(NO ˉ 3,NO ˉ 2,NH 3,soluble reactive phosphorus,total dissolved nitrogen,total dissolved phosphorus,dissolved oxygen,chemical oxygen demand,temperature,salinity,and chlorophyll a concentration) and microbial abundance(total viruses,total bacteria,P rochlorococcus,Synechococcus,and eukaryotes) were measured in the upper and lower layers at four sampling sites in the research area. In the direct viral counts,cyanomyoviruses accounted for 1.92% to 10% of the total viral community. A phylogenetic analysis showed that the g 20 sequences in the Shantou coastal waters were very diverse,distributed in eight distinct operational taxonomic units,including the newly formed Cluster W. The g 20 gene copies inferred from real time PCR assay indicated that cyanomyoviruses were correlated significantly with the heterotrophic bacteria numbers and the nitrate and chlorophyll a concentrations. These results suggest that cyanomyoviruses are ubiquitous and are an abundant component of the virioplankton in Shantou coastal waters.  相似文献   

不同培养方式及换水频率对坛紫菜(Porphyra haitanensis)自由丝状体生长及藻胆蛋白含量的影响     

丁兰平  颜泽伟  张全亮  徐佩杭  黄冰心 《海洋学报》2015,37(6):114-119

本实验以坛紫菜自由丝状体为材料,研究不同培养方式(静置、摇床、通气)和换水频率(5d、7d、10d、20d)对丝状体生长及藻胆蛋白(PE、PC)含量变化的影响。研究结果表明:坛紫菜自由丝状体在摇床培养条件下生长较好,特定生长速率可达10.832 7%~10.891 5%;摇床培养下的丝状体PE含量相对静置培养的较高一些,通气培养的相对较低,最高为36.816 8mg/g;静置条件下培养的丝状体PC含量最高,摇床培养次之,通气培养最低,最低为1.082 4mg/g。相同培养方式下,换水频率对丝状体特定生长速率影响不大;除通气培养外,换水频率20d其PE、PC含量较高。方差分析结果表明:实验范围内的培养方式及换水频率对丝状体特定生长速率大小、PE和PC含量不存在交互作用。本文实验结果对坛紫菜自由丝状体产业化应用于食品加工、色素提取等产业提供了实验理论和依据。  相似文献   

江苏近岸紫菜养殖筏架区定生绿藻群落结构及其受控因素   总被引：3，自引：1，他引：2  

李艳  肖洁  丁兰平  王宗灵  宋伟  方松  范士亮  李瑞香  张学雷 《海洋学报(英文版)》2015,34(8):93-99

基于2010年10月至2011年4月对苏北紫菜养殖筏架区定生绿藻及环境因素调查数据,分析研究了紫菜筏架上定生绿藻种群结构和数量变化及重要环境因子对其的影响并估算了调查区绿藻总生物量.结果表明,筏架上定生绿藻种类有浒苔(Ulva prolifera)、盒管藻(Capsosiphon groenlandicus)、缘管浒苔(Ulva linza)、肠浒苔(Ulva intestinalis)、条浒苔(Ulva clathrata)和扁浒苔(Ulva compressa).绿藻生物量变化呈倒抛物线型,4月份为主高峰14898吨,11月份为次高峰2034吨,2月份最低,仅为729吨,3-4月份绿藻几乎呈暴发性增长.种类多样性随季节变化有很大差异,养殖筏架刚入海的9、10月份,绿藻种类丰富,生物多样性高;12月至笠年2月,尽管生物量很低,但仍是多种并存;3-4月份随着生物量的猛增,种类多样性降至最低,盒管藻优势地位明显,生物量比例最大能到80%,浒苔比例呈指数增长,达20%～40%.水温对绿藻生物量及种类演替有直接调控作用,在水温< 10℃时,绿藻即能快速生长;而盐度作用不明显.开展紫菜筏架上定生绿藻群落动态变化及其生物量的估算,为追溯南黄海大规模绿潮发源地提供佐证,为绿潮预防和治理提供基础数据支撑.  相似文献   

海洋红藻岩生多管藻绒毛变种Polysiphonia scopulorum var. villum形态学研究          下载免费PDF全文

丁兰平  钟丽敏  谭华强  黄冰心 《热带海洋学报》2015,34(1):42-48

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Effects of the pharmaceutical fluoxetine in spiked-sediments on feeding activity and growth of the polychaete Capitella teleta     

《Marine environmental research》2013

The marine–estuarine polychaete Capitella is an indicator of organic pollution and plays important roles in sewage waste cycling. The antidepressant fluoxetine can be accumulated in streams and sewage effluents and it could pose a hazard to infauna. Effects of fluoxetine on feeding and growth of Capitella teleta were investigated through the exposure to 0, 0.001, 0.03, 0.3 and 3.3 μg/g dry weight sediment-spiked fluoxetine during 18 days. No effects of fluoxetine concentrations were observed on egestion rates, body weight and size-specific egestion rates. Fluoxetine favoured the occurrence of males with abnormal genital spines. This suggests that fluoxetine can have important reproductive implications. Further studies are recommended to assess potential detrimental effects on benthic infauna inhabiting close to sewage treatment plants.  相似文献   

Settlement Mode Analysis for An Immersed Tube Tunnel Considering A Nonuniform Foundation Under Tidal Load     

Wang  Yan-ning  Zhou  Huan-zhu  Wang  Le-chen 《中国海洋工程》2022,36(3):427-438

Immersed tube tunnels are usually placed on soft soil layers in cross-sea tunnelling engineering. Owing to the influence of stratum conditions and slope design, the longitudinal distribution of substratum layers is generally uneven. Thus, the inhomogeneous deformation of the element-joint becomes the key factor in the failure of the immersed tube tunnel. Therefore, a corresponding calculation method for joint deformation is needed to explore the deformation law of immersed tube tunnels. By constructing a three-section immersed tube tunnel analysis model (TTM), the relationship between the two types of deformation of the immersed tube tunnel structure in a longitudinal nonuniform soft soil foundation is described, and the deformation characteristics of the immersed structure under different boundaries are discussed. Based on the mechanical behaviour of the joint and foundation, according to the Timoshenko beam on the Vlasov two-parameter foundation (VTM), considering the tidal cyclic load during the operation and maintenance period, an example analysis is given. Moreover, the deformation characteristics and development trend of the immersed tube tunnel under the influence of different soil layers are discussed. The obtained results have a certain guiding significance for the deformation calculation of immersed tube tunnels.

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Influence of sequential tropical cyclones on phytoplankton blooms in the northwestern South China Sea     

Wang  Tongyu  Zhang  Shuwen  Chen  Fajin  Ma  Yonggui  Jiang  Chen  Yu  Jie 《中国海洋湖沼学报》2021,39(1):14-25

Upper ocean responses to the passage of sequential tropical cyclones over the northwestern South China Sea(SCS) in 2011 were investigated using satellite remote sensing data,Argo reanalysis data,and an array of mooring data.We found that the sea surface low temperature region lasted for more than 38 days and two phytoplankton blooms occurred after the passage of sequential tropical cyclones.The upper ocean cooling reached 2-5℃ with a right-side bias was observed along the typhoon track to about 200 km.The maintenance of low temperature region and the two phytoplankton blooms were mainly driven by upwelling and near-inertial turbulence mixing induced by the sequential tropical cyclones.The first phytoplankton bloom appeared on the 7~(th) day after the passage of the three tropical cyclones,and the chlorophyll-a(chl-a) concentration increased by 226%,which may be mainly driven by typhoons induced upwelling.The second phytoplankton bloom occurred on the 30~(th) day,the chl-a concentration increased by290%.Further analysis suggested that only the typhoons with similar characteristics as Nesat and Nalgae can induce strong near-inertial oscillation(NIO).Strong turbulent mixing associated with the near-inertial baroclinic shear instability lasted for 26 days.The measured mean eddy diffusivity in the upper ocean was above 10~(-4) m~2/s after typhoon Nesat.Enhancement of the turbulent mixing in the upper ocean helped to transport nutrient-rich cold waters from the deep layer to the euphotic layer,and is a maj or mechanism for the long-term maintenance of low temperature region as well as the second phytoplankton bloom.  相似文献   

Widespread distribution and molecular diversity of diatom frustule bound aliphatic long chain polyamines (LCPAs) in marine sediments     

《Organic Geochemistry》2012

The siliceous skeletal remains of diatoms are a primary component of sediments, covering vast areas of the sea floor. We used high performance liquid chromatography–electrospray ionization–tandem mass spectrometry (HPLC–ESI–MSⁿ) under high resolution time-of-flight MS (TOF-MS) conditions, as well as nuclear magnetic resonance spectroscopy (NMR) and Fourier transform infrared spectroscopy (FTIR), to investigate the distribution and molecular diversity of long chain polyamines (LCPAs) entrapped in sedimentary diatom biosilica in sediments from the Indian sector of the Southern Ocean, the Bering Sea and the Northeast Pacific. This revealed the existence of complex polyamine populations with both known and unknown structural features, including the presence of ketone and carboxylic acid groups in the LCPA backbone, functional groups that likely modulate the silica precipitating properties of LCPAs in the environment and which may constitute points of initial silica deposition (Wallace, A.F., De Yoreso, J.J., Dove, P.M., 2009. Kinetics of silica nucleation on carboxy- and amine-terminated surfaces: insights for biomineralization. Journal of the American Chemical Society 131, 5244–5250). Although various LCPA species overlapped between samples (putrescine-based LCPAs with various degrees of methylation and N-methyl propylamine repeat units were ubiquitous in all samples), clear regional differences were shown in composition and structural characteristics, likely reflecting the various diatom species contributing to the total LCPA pool for each sedimentary environment. We further show, using scanning electron microscopy and energy dispersive X-ray analysis (SEM–EDX) that LCPAs purified from diatom skeletal remains rapidly re-direct the formation of silica nanospheres in vitro, and become embedded in the precipitated silicified matrix, forming an LCPA–silica composite material. The results show that aliphatic polyamines produced by diatoms for morphogenesis of their cell walls are widespread in sediments where the fossil frustules are preserved. These newly assigned components constitute useful biological markers of diatom input to sediments.  相似文献   

两个龙须菜新品系经济性状及琼胶特性的初步研究   总被引：1，自引：0，他引：1  

陈伟洲  徐涤  王亮根  孟琳  杜虹  张学成 《中国海洋大学学报(自然科学版)》2009,39(3)

2008年2～5月在广东省南澳县深澳海区对选育的龙须菜新品系07-1,07-2和对照组福建宁德栽培的981品系进行海上栽培比较试验,测定了3个品系的藻体日平均生长速率,试验了敌害生物(藻钩虾和团水虱)对3个品系的影响,比较了琼胶特性.结果显示,在水温11～20 ℃,盐度32.4～32.8的条件下,品系07-1,07-2的藻体日平均生长速率分别为3.8%/d和4.1%/d,宁德栽培品系的藻体日平均生长速率为3.2%/d.品系07-1,07-2的生长适应水温下限为16 ℃,比宁德栽培品系降低了2 ℃.与宁德栽培的981品系相比较,选育的龙须菜品系07-1,07-2对藻钩虾和团水虱有较强的耐受能力.品系07-1,07-2的琼胶含量比福建宁德栽培的981品系分别提高10.0%和14.4%,凝胶强度也分别提高了5.46%和14.5%,表明2个新品系具有潜在的推广应用价值.  相似文献   

Identification of geological characteristics from construction parameters during shield tunnelling     

Yan  Tao  Shen  Shui-Long  Zhou  Annan 《Acta Geotechnica》2023,18(1):535-551

Acta Geotechnica - This paper proposes a framework to identify geological characteristics&nbsp;(GC) based on borehole data and operational data during shield tunnelling using a fuzzy C-means...  相似文献