基于Cyt b基因序列的南海北部蓝圆鲹群体遗传多样性研究          下载免费PDF全文

牛素芳  吴仁协  张丽艳  张浩冉  梁锐  李忠炉 《应用海洋学学报》2018,37(2):263-273

为了解南海北部蓝圆鲹(Decapterus maruadsi)的群体遗传变异特征,本研究利用线粒体DNA Cyt b基因部分序列对9个群体共203个个体进行群体遗传多样性和遗传结构分析.结果显示:在722 bp长的Cyt b部分序列中,共检测出52个多态位点,定义25个单倍型;群体的单倍型多样性(h)为0.577±0.036,核苷酸多样性(π)为0.001 55±0.001 12,整体遗传多样性呈中等偏低水平,其中雷州半岛和海南岛以东5个群体的遗传多样性水平明显高于以西的4个北部湾群体.单倍型系统发育树和网络图均未表现出与地理位置相对应的谱系结构,单倍型网络图呈以主体单倍型为中心的星状结构.群体间的Fst值为-0.077~0.018,且统计检验均不显著(p0.05).分子方差分析(AMOVA)表明,遗传变异全部来源于群体内个体间.Tajima's D值和Fu's Fs值均为显著负值,核苷酸不配对分布呈明显的单峰分布.南海北部蓝圆鲹群体约在29 000a前可能发生过扩张事件,导致遗传多样性呈现高h、低π模式;9个群体间的遗传分化并不显著,符合是一个随机交配种群的假设,但9个群体的遗传多样性水平却表现出明显的地理趋势,提示将南海北部蓝圆鲹作为单一种群进行渔业管理需持谨慎态度.  相似文献   

深圳杨梅坑海域造礁石珊瑚共生藻分类和遗传多样性研究          下载免费PDF全文

陈文静  佟飞  陈丕茂  朱爱意  张露  李丹丹  龙鑫玲 《应用海洋学学报》2019,38(2):284-291

通过核糖体ITS(Internal Transcribed Spacer)的核苷酸序列研究了深圳杨梅坑海域20种47个造礁石珊瑚样本的共生藻。通过ITS序列分析,与GenBank上的4种不同的共生藻构建Neighbor-Joining聚类树,进行石珊瑚共生藻分类和遗传多样性分析。结果表明,该海域的造礁石珊瑚共生藻属于2种不同的种类(亚系群),19个样品属于C1亚系群共生藻,1个样品属于C15亚系群共生藻,C1和C15两个亚系群共生藻之间的遗传距离为0. 01。将深圳杨梅坑海域得到的ITS序列与NCBI数据库上的福建东山海域、广东徐闻地区的C1系共生藻进行比对,只有深圳杨梅坑海域藻类样本平均(A+T)碱基含量为49. 4%,(A+T)含量小于(G+C)含量。构建Neighbor-Joining聚类树表明,深圳杨梅坑海域石珊瑚共生藻与福建东山海域的亲缘关系较近,而与广东徐闻地区的亲缘关系较远,地理隔离是主要的因素。  相似文献   

基于不同区域层级的文化产业集聚研究   总被引：1，自引：0，他引：1  

陶金  罗守贵 《地理研究》2019,38(9):2239-2251

利用企业层面数据,从市域和省域两个尺度研究中国文化产业的集聚特征,并揭示集聚对文化企业生产率的影响。应用一致性方法估计了中国文化企业的生产函数,并计算企业层面全要素生产率估计值,并从专业化和城市化两个角度研究集聚对于文化企业的影响。结果表明：① 地级市尺度下,文化产业专业化经济并不显著,但却存在城市化经济;② 在更大的省域层面,则呈现出专业化经济显著,多样化经济弱化的特征;③ 行业和区域异质性回归显示了结果的稳健性,核心文化产业的产业集聚经济更显著;④ 在知识溢出效应占主导的文化集聚中,通信基础设施良好的省份被赋予了创意交流的便利性,因而拥有更强的集聚经济。  相似文献   

海面温度异常时间序列的谱分析     

杜军  修延霞  罗爱军  晋海龙 《测绘信息与工程》2009,34(6):38-39

利用了傅里叶功率谱和小波功率谱分析方法,分析了太平洋中、东部地区海面温度异常时间序列的时频特征,发现了海面温度异常信号具有2~6年左右的周期特征。  相似文献   

杭州湾上海石化沿岸潮间带生态环境分析   总被引：1，自引：0，他引：1  

张进龙 《海洋湖沼通报》2008,(1):74-79

通过对1993～2002年杭州湾上海石化沿岸潮间带底栖动物群落结构的分析,得出上海石化处理达标后排放的工业废水对此段潮间带生态环境影响的范围和程度。自2002年到现在为止,本潮间带的底栖动物群落结构呈现一种适应性广、耐污染的结构组成.其生物多样性指数值在2.00～3.50之间。  相似文献   

Perturbed solution of sea-air oscillator for the E1 Ni(n)o/La Ni(n)a-Southern Oscillation mechanism     

ZHOU Xianchun  LIN Yihua  WANG Hui  MO Jiaqi 《海洋学报(英文版)》2009,28(3)

A class of coupled system of the E1 Ni(n)o/La Ni(n)a-Southern Oscillation (ENSO) mechanism is studied. Using the perturbed theory, the asymptotic expansions of the solution for ENSO model are obtained and the asymptotic behavior of solution for corresponding problem is considered.  相似文献   

Characterization of marine microplankton communities of Qingdao coastal areas using randomly amplified polymorphic DNA（RAPD）   总被引：1，自引：1，他引：0  

GAO Shan  HU Xiaozhong  CHEN Zigui  XU Henglong  YI Zhenzhen  LIN Xiaofeng  LIJiqiu 《海洋学报(英文版)》2009,28(1)

Microplankton communities of three coastal sites of Qingdao, Shandong Province, China were investigated using RAPD (random amplified polymorphic DNA) molecular markers and morphological observations. Eight RAPD-primers were selected to amplify the DNA polymorphy. The genetic distances inferred from the pairwise similari-ties were calculated for the phylogenetic tree construction. Meantime, the traditional microscopic determination, a way of visualizing the species composition, was performed to detect the major taxa of microplanktons from all samples. Results showed that: (1) the band sharing index values were in the range of 0. 504 2-0. 763 2 among samples from the same sampling site at different time scales, while 0.406 5-0.685 7 among the samples from different stations at the same time scales, indicating that spatial variations of microplankton communities were more pronounced than temporal ones; (2) samples from the same station basically clustered together, cor-responding to the geographic distribution of the sampling sites; (3) diversity derived from genetic and morpho-logical data did not correspond with each other well.  相似文献   

Biodiversity patterns of free-living marine nematodes in a tropical bay: Cienfuegos, Caribbean Sea   总被引：2，自引：0，他引：2  

M. Armenteros  A. Ruiz-Abierno  R. Fernndez-Garcs  J.A. Prez-García  L. Díaz-Asencio  M. Vincx  W. Decraemer 《Estuarine, Coastal and Shelf Science》2009,85(2):179-189

Spatial and temporal biodiversity patterns of free-living marine nematodes were studied in Cienfuegos Bay, a tropical semi-enclosed basin in the Caribbean Sea. Taxonomic (to species level) and functional (biological trait) approaches were applied for describing the assemblage structure and relating it to abiotic environment based on a sampling scheme in six subtidal stations and three months. Biological trait approach added relevant information to species pattern regarding relationships between diversity patterns and the abiotic environment. The most common morphotypes were deposit feeding nematodes, with colonising abilities of 2–3 (in a scale from 1 to 5), tail conical cylindrical or filiforme and body slender; and their abundance were correlated with depth, organic matter and silt/clay fraction. In spite of a high turnover of species, functional diversity of assemblages did not change notably in space and time. A result probably due to sampling of the habitat pool of species and to low heterogeneity of the studied muddy bottoms. Chemical pollution (organic enrichment and heavy metals) and hydrodynamic regime possibly drove the biodiversity patterns. Spatial distribution of assemblages support the existence of two well differentiated basins inside the bay, the northern basin more polluted than the southern one. The low hydrodynamic regime would determine a poor dispersion of nematodes resulting in high spatial variance in the assemblage structure; and also the associated hypoxic conditions and pollutants in sediments can explain the dominance of tolerant nematode species such as Daptonema oxycerca, Sabatieria pulchra, Terschellingia gourbaultae, and Terschellingia longicaudata. A comparison of spatial–temporal patterns of biodiversity between Cienfuegos Bay and other semi-enclosed bays in temperate regions suggests several similarities: nematode assemblages are strongly influenced by anthropogenic disturbance, temporal trends are weak or overridden by spatial ones, and few cosmopolitan genera/species tolerant to pollution and hypoxic conditions are dominant.  相似文献   

Observations of the North Equatorial Current,Mindanao Current,and Kuroshio current system during the 2006/07 El Niño and 2007/08 La Niña   总被引：1，自引：0，他引：1  

Yuji Kashino  Norievill España  Fadli Syamsudin  Kelvin J. Richards  Tommy Jensen  Pierre Dutrieux  Akio Ishida 《Journal of Oceanography》2009,65(3):325-333

Two onboard observation campaigns were carried out in the western boundary region of the Philippine Sea in December 2006 and January 2008 during the 2006/07 El Niño and the 2007/08 La Niña to observe the North Equatorial Current (NEC), Mindanao Current (MC), and Kuroshio current system. The NEC and MC measured in late 2006 under El Niño conditions were stronger than those measured during early 2008 under La Niña conditions. The opposite was true for the current speed of the Kuroshio, which was stronger in early 2008 than in late 2006. The increase in dynamic height around 8°N, 130°E from December 2006 to January 2008 resulted in a weakening of the NEC and MC. Local wind variability in this region did not appear to contribute to changes in the current system.  相似文献   

Genetic diversity analysis with ISSR PCR on green algae Chlorella vulgaris and Chlorella pyrenoidosa     

沈颂东 《中国海洋湖沼学报》2008,26(4)

In the present study, genetic polymorphism and diversity in unicellular clones of Chlorella vulgaris Beijerinck and Chlorella pyrenoidosa Chick were studied with Inter Simple Sequence Repeats PCR (ISSR PCR). Samples including four clones of C. vulgaris and three clones of C. pyrenoidosa were purified by single-clone-choice method. For four C. vulgaris unicellular clones, the total number of the bands scored for 18 primers was 298; and the number of the polymorphic bands was 118, of which 39.6% were polymorphic. The size of PCR products ranged from 200 to 2 500 bp. The total number of bands scored for 18 primers, the number of polymorphic bands and the percentage of three C. pyrenoidosa unicellular clones was 194.83 and 30.8%, respectively. POPGENE analysis show that the average Nei genetic diversity (h *) and Shannon index of diversity (I *) in the four C. vulgaris unicellular clones was 0.2181 and 0.3208, respectively, which is slightly higher than those of the three C. pyrenoidosa unicellular clones (0.190 3 and 0.274 8), which agreed with the percentage of polymorphic bands in the mixed samples of the two species. The results suggest that ISSR is a useful method to Chlorella for intra-species genetic analysis.  相似文献