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101.
麻痹性贝毒(Paralytic Shellfish Poisoning,PSP)毒素由石房蛤毒素(saxitoxin,STX)及其衍生物组成,目前己发现20余种,在赤潮研究、分子生物学和神经生物学基础研究、医药、军事防化等方面都有应用潜力[其结构、类型和应用见本集刊王云峰等(2003)“麻痹性贝毒毒素的应用研究进展”一文]。由于PSP毒素的稀有来源和国际社会对STX交易的禁止,限制了国内PSP毒素应用及研究的全面深入展开,因此本文作者对PSP毒素进行了制备,并采用不同方法对制备的PSP毒素进行了研究。
PSP毒素能够选择性地可逆抑制可兴奋膜的电压依赖钠离子通道的开放,从而阻止神经冲动的发生和传导,使神经、肌肉丧失兴奋性(Frace et al.,1986;Penzotti et al.,1998)。本文利用神经束膜下记录和全细胞膜片钳技术,报道了从塔玛亚历山大藻(Alexandrium tamarense)中提取的PSP粗毒素对小鼠运动神经末梢膜电流和NG108-15细胞钠离子通道的作用研究结果,并与STX标准毒素的作用结果进行了比较。
NG108-15细胞是由小鼠神经母细胞瘤和大鼠胶质细胞瘤融合的杂交细胞,经分化剂分化后,显示诸+L1196如兴奋性、合成和释放乙酰胆碱、与培养肌细胞形成突触联系等多种神经细胞的基本特性(Hamprcht,1977)和Na+、K+、Ca2+等多种离子通道,已作为神经细胞模型被广泛应用于分析药物对离子通道作用的研究(Enomoto et al.,1992; Docherty et al.,1992;Shi et al.,1993;Hu et al.,1997a;Hu et al.,1997b);发育出具有Na+内流支持的锋电位(Hamprecht,1977),该电位是分析作用于膜钠离子通道药物的好材料。 相似文献
102.
We have examined wind-induced circulation in the Sea of Okhotsk using a barotropic model that contains realistic topography
with a resolution of 9.25 km. The monthly wind stress field calculated from daily European Centre for Medium-Range Weather
Forecasting (ECMWF) Re-Analysis data is used as the forcing, and the integration is carried out for 20 days until the circulation
attains an almost steady state. In the case of November (a representative for the winter season from October to March), southward
currents of velocity 0.1–0.3 m s−1 occur along the bottom contours off the east of Sakhalin Island. The currents are mostly confined to the shelf (shallower
than 200 m) and extend as far south as the Hokkaido coast. In the July case (a representative for the summer season from April
to September), significant currents do not occur, even in the shallow shelves. The simulated southward current over the east
Sakhalin shelf appears to correspond to the near-shore branch of the East Sakhalin Current (ESC), which was observed with
the surface drifters. These seasonal variations simulated in our experiments are consistent with the observations of the ESC.
Dynamically, the simulated ESC is interpreted as the arrested topographic wave (ATW), which is the coastally trapped flow
driven by steady alongshore wind stress. The volume transport of the simulated ESC over the shelf reaches about 1.0 Sv (1
Sv = 106 m3s−1) in the winter season, which is determined by the integrated onshore Ekman transport in the direction from which shelf waves
propagate.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
103.
Breaking wave induced nearsurface turbulence has important consequences for many physical and biochemical processes including water column and nutrients mixing,heat and gases exchange across air-sea interface.The energy loss from wave breaking and the bubble plume penetration depth are estimated.As a consequence,the vertical distribution of the turbulent kinetic energy(TKE),the TKE dissipation rate and the eddy viscosity induced by wave breaking are also provided.It is indicated that model results are found to be consistent with the observational evidence that most TKE generated by wave breaking is lost within a depth of a few meters near the sea surface.High turbulence level with intensities of eddy viscosity induced by breaking is nearly four orders larger than υwl(=κuwz),the value predicted for the wall layer scaling close to the surface,where uw is the friction velocity in water,κ with 0.4 is the von Kármán constant,and z is the water depth,and the strength of the eddy viscosity depends both on wind speed and sea state,and decays rapidly through the depth.This leads to the conclusion that the breaking wave induced vertical mixing is mainly limited to the near surface layer,well above the classical values expected from the similarity theory.Deeper down,however,the effects of wave breaking on the vertical mixing become less important. 相似文献
104.
M.L. Botter-Carvalho P.J.P. Santos P.V.V.C. Carvalho 《Estuarine, Coastal and Shelf Science》2007,71(3-4):508-516
We describe the structure, reproductive cycle, fecundity, growth, and mortality of a harvested population of the ghost shrimp Callichirus major. Samples were collected at monthly intervals from September 1999 to October 2000 on an urban sandy beach (08°11′S 34°55′W) in northeastern Brazil. During this period the sex ratio did not differ significantly from 1:1 (0.98 M: 1 F). Minimum and maximum sizes of the Dorsal Oval were 2.59 and 12.19 mm for males and 4.46 and 12.62 mm for females, respectively. Ovigerous females were found throughout the period, except between August and September 2000. Maximum lifespan was estimated as 3.3 and 3.4 years for females and males, respectively. This northeastern population differed from others previously studied in southern and southeastern Brazil, in regard to sex ratio, maximum attained size, maturation size, period and duration of the reproductive cycle, and fecundity. We interpret these regional differences as evidence for over-fishing at the study site, and suggest that large-scale management plans for callianassid populations should use regional population parameters. 相似文献
105.
106.
根据Taylor的湍流扩散理论和Hay、Pasquill提出的拉格朗日流和欧拉流的相关函数的相似性,用黄河三角洲埕北海域的实测资料,按四种不同方案计算出该海域的水平扩散系数和谱密度分布。指出:该海区潮流扩散系数主要受潮流的影响,其分布特征与潮流的分布基本一致,所求的水平扩散系数主要是由周期性的潮运动给予潮流扰动所致。方案4是通过富氏变换滤波消去了周期性潮流扰动,本方案计算的水平扩散系数是由周期为6h以下的小尺度涡提供能量。 相似文献
107.
An instrument which measures the velocity and direction of benthic water currents at depths of up to 80 m is described. Characteristics of this meter include: (i) readings are not affected by water flow during sinking or retrieval; (ii) it is portable; (iii) it operates unattended; (iv) it is inexpensive; and (v) it requires only simple maintenance. A timing circuit which operates two time periods in succession is used to activate and de-activate a revolution counter and a fluidfilled compass which are mounted on a special frame and vane system. In the field, the instrument accurately measured average current velocities of up to 6 m s−1. Data are presented which illustrate the usefulness of this instrument in describing the velocity and direction of benthic currents along the east coast of Australia and demonstrate a positive correlation between benthic current velocity and catch rates of the commercially-exploited spanner crab Ranina ranina. 相似文献
108.
在连云港近岸海域计算潮流场基础上建立拉格朗日余流模型,并对连云港市两大堤建成前后的拉格朗日余流变化进行了分析,且选择有代表性的排污口进行了数值跟踪。 相似文献
109.
110.
Responses of a Mediterranean soft bottom community to short-term (1993-1996) hydrological changes in the Rhone river 总被引:1,自引:0,他引:1
The polychaete fauna of muddy bottoms off the Rhone delta (NW Mediterranean) was seasonally sampled at two sites at 70 m depth, from 1993 to 1996. During this period, five severe flood events occurred. A clustering analysis (distance coefficient of Whittaker) and three way fixed factor ANOVAs (site x season x year) showed strong year-to-year changes in species density and community structure, changes that masked seasonal variations. Total density increased by a factor of 3 and density of most species significantly increased following the first flooding event. Changes in the community structure were due to the successive dominance of a few species. The opportunistic species, such as Cossura sp., Mediomastus sp. and Polycirrus sp., exhibited peaks in density 1-3 months after the flood. These peaks were followed by a drastic decline. For species with a long life span, such as Sternaspis scutata, a slower but continuous increase in density was observed which was maintained for several months. The density fluctuations of these species exhibited a good correlation with river flow with time lags of 1-2 years. The successional dynamics observed are explained according to the geographical origin of the floods and the biology and feeding ecology of species. 相似文献