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31.
32.
We isolated 4 Norwalk-like viruses (NLVs) contaminated oysters from 33 Chinese oysters collected from local commer-cial sources of Shandong Province. After amplification of the RNA-dependent RNA polymerase (RdRp) region of NLVs genomes with RT-PCR, the open reading frame 1 (ORF1) of the RdRp was sequenced and subjected to multiple-sequence alignment. The re-suits showed that NLVs in the four isolates belong to genogroup Ⅱ. The sequence comparison showed that the similarity between four Chinese oyster isolates were higher than 99.0%, which indicated that NLVs prevalent in close areas have high homogeneity in genome sequences. In addition, the most conserved sequences between diverse NLVs were used to design primers and TaqMan probes, then the real-time quantitative PCR assay was performed. According to the standard curve of GII NLVs, the original amounts (copies) of NLVs in positive patient's fecal isolate, positive Japanese oyster isolate, and the Chinese oyster isolate were 8.9×108, 1.25×108 and 4.7×101 respectively. The detecting limit of NLVs was 1×101 copies. This study will be helpful for routine diagnosis of NLVs pathogens in foods and thus for avoiding food poisoning in the future. 相似文献
33.
苹果手机零部件全球价值链的价值分配与中国角色演变 总被引:3,自引:2,他引:1
产品内分工已成为支配当代企业分工与产品生产的重要模式。研究智能手机生产网络价值分配格局,对辨析中国大陆企业在移动通讯产业价值链中所处地位与演进具有重要意义。论文利用2012—2017年苹果公司供应商数据,以其零部件价值含量与技术要求为测度标准,分析了苹果手机零部件全球价值链的分配特征以及中国大陆企业在其中的地位变化。发现:高价值环节的供应商主要以美国、日本、韩国为主,而中低价值环节则以中国台湾、日本和中国大陆供应商为主,且中国大陆与中国台湾在中间环节占据重要地位。处于价值链高端的供应商分支数量在中国大陆和美国缩减明显,日本、韩国较为稳定,中国台湾增加明显。在价值链的中低端环节,供应商分支趋向中国大陆集中。印度、巴西以及越南等地成为新的中低端价值环节转移承接地。近年来,中国大陆在苹果手机价值链的高端环节得到了提升,长三角、珠三角、京津冀、成渝、中原地区等地逐渐形成了苹果供应商分支的集聚区域,呈现出零部件生产的区域分工特征。长三角(上海-苏州为核心)与珠三角(深圳-东莞为核心),承接了价值链多个生产环节,是中国大陆嵌入苹果手机生产网络的主要阵地。成渝地区既承接了芯片类企业布局,也承接了价值链低端的组装代工和包装服务环节,呈现出在价值链两端双向承接的特征。环渤海、福建及湖南等地主要承接苹果手机价值链的中间环节,而中原地区主要以苹果手机组装与包装服务为主,承接价值链的低端环节。最后,论文从创新驱动、政府作用与外资等方面探讨了影响中国大陆地位提升的因素。 相似文献
34.
传统村落景观基因信息链与自动识别模型构建——以陕西省为例 总被引:2,自引:0,他引:2
传统村落作为活的文化遗产,承载了大量历史记忆,是地域文化景观基因识别与模型构建研究的重要切入点。以陕西省71个国家级传统村落为例,基于景观基因理论建立陕西省传统村落景观基因识别体系,识别出陕西省传统村落景观基因特征;运用类型学原理和N级编码理论对景观基因进行编码,构建陕西省传统村落景观基因信息链并生成基因谱系;借鉴生物学中“胞-链-形”DNA碱基序列模型,提取出环境基因、建筑基因、农耕文化基因和宗族文化基因四个公共基因作为景观基因元(胞),以村内道路系统作为基因链,构建传统村落景观基因DNA模型与自动识别模型,以此对传统村落的区位、类型、特征和文化基因进行自动识别。为传统村落景观基因信息有效传承与存储,以及乡村建设动态发展提供理论借鉴。 相似文献
35.
Tingting Xu Jay Gao Giovanni Coco 《International journal of geographical information science》2019,33(10):1960-1983
Accurate simulations and predictions of urban expansion are critical to manage urbanization and explicitly address the spatiotemporal trends and distributions of urban expansion. Cellular Automata integrated Markov Chain (CA-MC) is one of the most frequently used models for this purpose. However, the urban suitability index (USI) map produced from the conventional CA-MC is either affected by human bias or cannot accurately reflect the possible nonlinear relations between driving factors and urban expansion. To overcome these limitations, a machine learning model (Artificial Neural Network, ANN) was integrated with CA-MC instead of the commonly used Analytical Hierarchy Process (AHP) and Logistic Regression (LR) CA-MC models. The ANN was optimized to create the USI map and then integrated with CA-MC to spatially allocate urban expansion cells. The validated results of kappa and fuzzy kappa simulation indicate that ANN-CA-MC outperformed other variously coupled CA-MC modelling approaches. Based on the ANN-CA-MC model, the urban area in South Auckland is predicted to expand to 1340.55 ha in 2026 at the expense of non-urban areas, mostly grassland and open-bare land. Most of the future expansion will take place within the planned new urban growth zone. 相似文献
36.
Examining the sensitivity of spatial scale in cellular automata Markov chain simulation of land use change 总被引:2,自引:0,他引:2
Hao Wu Zhen Li Keith C. Clarke Wenzhong Shi Anqi Lin 《International journal of geographical information science》2019,33(5):1040-1061
Understanding the spatial scale sensitivity of cellular automata is crucial for improving the accuracy of land use change simulation. We propose a framework based on a response surface method to comprehensively explore spatial scale sensitivity of the cellular automata Markov chain (CA-Markov) model, and present a hybrid evaluation model for expressing simulation accuracy that merges the strengths of the Kappa coefficient and of Contagion index. Three Landsat-Thematic Mapper remote sensing images of Wuhan in 1987, 1996, and 2005 were used to extract land use information. The results demonstrate that the spatial scale sensitivity of the CA-Markov model resulting from individual components and their combinations are both worthy of attention. The utility of our proposed hybrid evaluation model and response surface method to investigate the sensitivity has proven to be more accurate than the single Kappa coefficient method and more efficient than traditional methods. The findings also show that the CA-Markov model is more sensitive to neighborhood size than to cell size or neighborhood type considering individual component effects. Particularly, the bilateral and trilateral interactions between neighborhood and cell size result in a more remarkable scale effect than that of a single cell size. 相似文献
37.
The factors of safety for stationkeeping systems in current standards (ISO, API) are not derived or validated using reliability analyses. As the oil and gas exploration and production is breaking new boundaries, deploying new floating systems and moving into regions with harsher environments, it is of paramount importance to understand what level of reliability these new marine structures are achieving. This paper presents a reliability analysis of the mooring system of a Floating LNG (FLNG) vessel permanently moored and permanently manned offshore North West Australia in a tropical cyclone environment. The reliability analysis addresses both the mooring chain and the pile foundation. The analysis accounts for the long term characteristics of the environment, including the short term variability, in response to a given sea state and the variability and uncertainty in strength of the mooring chain and the pile. The stationkeeping system was analysed using detailed time domain simulations, capturing system non-linearities and low frequency oscillations as well as wave frequency responses and, thereby, reducing modelling uncertainties to a minimum.It is found that for the conditions modelled, neither the chain nor the pile meet a target reliability of 10−4/annum using the factors of safety commonly used in design following current ISO and API standards. New factors of safety are proposed to achieve this target reliability. For the pile design, one complicating factor is that current design standards do not explicitly define the exceedance probability that should be associated with the characteristic value of the undrained shear strength to be used in the design. It is demonstrated that the required factor of safety is crucially dependent on the definition of this characteristic value and on the level and the type of uncertainty in the soil strength profile. A recommendation is made regarding the definition of this characteristic value and the associated factor of safety. Furthermore, it is found that designing the mooring system to an environmental condition with a return period of 10,000 yr (as an Abnormal Limit State event), and setting the factor of safety to unity, meets the target reliability of 10−4/annum for the pile, if the characteristic undrained shear strength is a lower bound, defined in this paper by the 10th percentile value. For the chain however, this target reliability is not achieved. 相似文献
38.
文章提出了一种识别混合层深度的人工智能方法。该方法在温度(密度)与压强(或深度)间建立线性模型, 并且将其系数和方差做成一组表征廓线特征的统计量。初始时为模型设定一个主观的先验分布, 在一个自海表向下移动的窗口内通过贝叶斯链式法则和最小描述长度原理学习新数据, 得到系数均值的最大后验概率估计。用F-检验识别系数发生突变的位置, 以此确定混合层的存在性及其深度。通过2017年2月太平洋海域的地转海洋学实时观测阵(Array for Real-time Geostrophic Oceanography, ARGO)数据进行测试, 并且以质量因子(Quality Index, QI)值作为判断识别混合层深度结果准确性的依据, 发现该方法相比于梯度法、阈值法、混合法、相对变化法、最大角度法和最优线性插值法在识别结果上具备更大的QI值。表明该方法能够准确识别混合层深度。 相似文献
39.
目前没有规范给出串锚的水平承载力计算公式,为了利用有限元法研究串锚水平承载力特性,采用相关理论计算以及室内试验的方法来验证有限元法模拟计算的可靠性。实践证明,有限元法具有较高的计算精度。利用有限元分析软件ABAQUS建立了串锚-土体模型,模拟串锚在水平荷载作用下破坏过程,研究了串锚在水平荷载作用下破坏机理,从而得到了串锚水平承载力特性。在相同的工况条件下,串锚的水平承载力与其锚链长度有关,在一定范围内,增加锚链的长度可以提高串锚的水平承载力;串锚的水平承载力不是相应的单个锚的水平承载力相加,其水平承载力小于相应的单个锚水平承载力之和,在进行串锚水平承载力设计计算时应给与相应的考虑。 相似文献
40.
Simulation of landscape spatial layout evolution in rural-urban fringe areas: a case study of Ganjingzi District 总被引:2,自引:0,他引:2
In recent years, the rapid expansion of urban spaces has accelerated the mutual evolution of landscape types. Analyzing and simulating spatio-temporal dynamic features of urban landscape can help to reveal its driving mechanisms and facilitate reasonable planning of urban land resources. The purpose of this study was to design a hybrid cellular automata model to simulate dynamic change in urban landscapes. The model consists of four parts: a geospatial partition, a Markov chain (MC), a multi-layer perceptron artificial neural network (MLP-ANN), and cellular automata (CA). This study employed multivariate land use data for the period 2000–2015 to conduct spatial clustering for the Ganjingzi District and to simulate landscape status evolution via a divisional composite cellular automaton model. During the period of 2000–2015, construction land and forest land areas in Ganjingzi District increased by 19.43% and 15.19%, respectively, whereas farmland, garden lands, and other land areas decreased by 43.42%, 52.14%, and 75.97%, respectively. Land use conversion potentials in different sub-regions show different characteristics in space. The overall land-change prediction accuracy for the subarea-composite model is 3% higher than that of the non-partitioned model, and misses are reduced by 3.1%. Therefore, by integrating geospatial zoning and the MLP-ANN hybrid method, the land type conversion rules of different zonings can be obtained, allowing for more effective simulations of future urban land use change. The hybrid cellular automata model developed here will provide a reference for urban planning and policy formulation. 相似文献