高通量测序分析北仑河口红树林潮间带底栖真核生物分布          下载免费PDF全文

吴鹏  时小军  吕向立  高阳  李海涛  张敬怀 《应用海洋学学报》2019,38(2):232-238

为了解红树林不同潮位沉积物中底栖真核生物群落分布,基于18S rRNA基因采用高通量测序方法分析了广西北仑河口陆缘、林中和海缘3个潮位红树林沉积物中底栖生物群落结构。结果表明,北仑河口潮间带红树林沉积物中底栖生物多样性丰富,Shannon-Wiener指数变化范围在6. 08～6. 73之间; PCA分析表明潮间带中底栖生物群落差异较大,陆缘红树林中扁形动物、节肢动物和软体动物相对丰度较高,林中区域中纤毛虫、环节动物和轮虫相对丰度较高,海缘红树林中硅藻相对丰度较高;红树林中主要OTUs有桡足类的太平洋纺锤水蚤(Acartia pacifica)、硅藻类的海链藻(Thalassiosira sp.)、纤毛虫类的前管虫(Prorodon teres)、多毛类的小头虫(Capitella sp.)。高通量测序方法能较全面反映红树林区微型/小型底栖生物群落,研究结果为丰富红树林底栖生物群落研究和解析底栖生物在红树林生态系统发挥的作用提供基础数据。  相似文献   

Numerical simulation and preliminary analysis of typhoon waves during three typhoons in the Yellow Sea and East China Sea     

WANG Ning  HOU Yijun  LI Shuiqing  LI Rui 《海洋湖沼学报（英文）》2019,(6):1805-1816

In this study,typhoon waves generated during three typhoons(Damrey(1210),Fung-wong(1416),and Chan-hom(1509))in the Yellow Sea and East China Sea were simulated in a simulating waves nearshore(SWAN)model,and the wind forcing was constructed by combining reanalyzed wind data with a Holland typhoon wind model.Various parameters,such as the Holland fitting parameter(B)and the maximum wind radius?,were investigated in sensitivity experiments in the Holland model that affect the wind field construction.Six different formulations were considered and the parameters determined by comparing the simulated wind results with in-situ wind measurements.The key factors affecting wave growth and dissipation processes from deep to shallow waters were studied,including wind input,whitecapping,and bottom friction.Comparison with in-situ wave measurements suggested that the KOMEN scheme(wind input exponential growth and whitecapping energy dissipation)and the JONSWAP scheme(dissipation of bottom friction)resulted in good reproduction of the significant wave height of typhoon waves.A preliminary analysis of the wave characteristics in terms of wind-sea and swell wave revealed that swell waves dominated with the distance of R to the eye of the typhoon,while wind-sea prevailed in the outer region up to six to eight times the R values despite a clear misalignment between wind and waves.The results support the hypothesis that nonlinear wave-wave interactions may play a key role in the formation of wave characteristics.  相似文献   

Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria     

YIN Qunjian  ZHANG Weijia  LI Xuegong  ZHOU Lihong  QI Xiaoqing  ZHANG Chan  WU Long-Fei 《海洋湖沼学报（英文）》2019,(1):210-222

Trimethylamine N-oxide(TMAO) is widely dispersed in marine environments and plays an important role in the biogeochemical cycle of nitrogen. Diverse marine bacteria utilize TMAO as carbon and nitrogen sources or as electron acceptor in anaerobic respiration. Alteration of respiratory component according to the pressure is a common trait of deep-sea bacteria. Deep-sea bacteria from dif ferent genera harbor high hydrostatic pressure(HHP) inducible TMAO reductases that are assumed to be constitutively expressed in the deep-sea piezosphere and facilitating quick reaction to TMAO released from ?sh which is a potential nutrient for bacterial growth. However, whether deep-sea bacteria universally employ this strategy remains unknown. In this study, 237 bacterial strains affliated to 23 genera of Proteobacteria,Bacteroidetes, Firmicutes and Actinobacteria were isolated from seawater, sediment or amphipods collected at dif ferent depths. The pressure tolerance and the utilization of TMAO were examined in 74 strains. The results demonstrated no apparent correlation between the depth where the bacteria inhabit and their pressure tolerance, regarding to our samples. Several deep-sea strains from the genera of Alteromonas, Halomonas,Marinobacter, Photobacterium, and Vibrio showed capacity of TMAO utilization, but none of the isolated Acinebacter, Bacillus, Brevundimonas, Muricauda, Novosphingobium, Rheinheimera, Sphingobium and Stenotrophomonas did, indicating the utilization of TMAO is a species-speci?c feature. Furthermore, we noticed that the ability of TMAO utilization varied among strains of the same species. TMAO has greater impact on the growth of deep-sea isolates of Vibrio neocaledonicus than shallow-water isolates. Taken together, the results describe for the ?rst time the TMAO utilization in deep-sea bacterial strains, and expand our understanding of the physiological characteristic of marine bacteria.  相似文献   

CCD astrometric observations of 2017 VR12, Camillo and Midas     

Zhen-Jun Zhang  Yi-Gong Zhang  Xiang-Ming Chen  Jian-Cheng Wang  Jie Su 《天文和天体物理学研究(英文版)》2019,(5):91-100

We have observed three near-Earth objects(NEOs), 2017 VR12, Camillo and Midas, during 2018.The observations were made with the 1-m telescope, operated by Yunnan Observatories, over two nights.Their precise astrometric positions are derived from 989 CCD observations. The theoretical positions of asteroids are retrieved from the Jet Propulsion Laboratory(JPL) Horizons System and Institut de M′ecanique C′eleste et de Calcul des ′Eph′em′erides(IMCCE). The positions of three asteroids are measured with respect to stars in the Gaia DR2 star catalog. For 2017 VR12, the means(O-C) of right ascension and declination are -0.090′′and-0.623′′respectively based on the published JPL ephemeris, but the corresponding means(O-C) are 3.122′′and-0.636′′based on the published IMCCE ephemeris. The great difference in declination could be explained by several factors.(1) The degraded CCD images caused by the fast apparent motion of the objects lead to a reduction in positioning accuracy.(2) The poor timing system may introduce systematic errors, especially in the high speed direction.(3) The asteroid may be perturbed by Earth when it approaches the Earth too closely. These astrometric results demonstrate that the centroid centering method can reduce the dispersion of non-Gaussian images as compared with the PSF modeling method.For Camillo and Midas, the astrometric results are consistent based on the two ephemerides. Implementing a high-precision timing system, and analyzing some astronomical effects and geometric distortions in CCD images should be carefully considered in future works.  相似文献   

建筑物侧面轮廓线约束的高分辨率遥感影像建筑物高度估算方法     

陈辉  张卡  宿东  王蓬勃 《测绘通报》2019,(9):34-37,72

针对现有利用阴影长度法提取建筑物高度时存在的阴影间相互遮挡问题,提出了一种基于建筑物侧面轮廓线进行建筑物高度估算的新方法。首先,利用RPC模型计算建筑物像点位移的方向与卫星成像角度,再将遥感影像进行旋转,使建筑物像点位移沿水平方向;然后,利用Canny算法进行轮廓检测,并构建一定长度的矩形形态学结构元素,对轮廓图像进行形态学开运算,以提取侧面轮廓线,再利用Hough变换与建筑物角点约束,对所提取的轮廓线进一步筛选;最后,根据卫星侧视成像时建筑物高度与像点位移的几何关系进行建筑物的高度估算。利用实际的高分辨率卫星影像对本文方法进行了验证,并与阴影法估算建筑物高度进行了对比。试验结果证明,利用建筑物侧面轮廓线进行建筑物高度估算平均误差可以达到0.7 m,且实际精度优于使用阴影法进行建筑物高度估算。  相似文献   

机载激光点云中高压电塔自动识别方法   总被引：2，自引：2，他引：0  

刘洋  杨必胜  梁福逊 《测绘通报》2019,(1):34-38

提出了一种基于格网特征的机载激光点云高压电塔自动识别方法。首先对机载激光点云数据进行滤波去噪处理;然后对点云数据进行规则格网化特征分析获得高压电塔粗识别区域;最后对粗识别区域进行外接邻域网格线性特征悬空点集检测以确定电塔识别结果,并以分层切片法分析获取电塔平面中心坐标。采用大型无人机实际线路巡检获取的机载点云数据对本文算法进行验证,试验结果表明本算法可实现高压电塔的快速自动识别,对无人机电力巡检智能诊断具有一定的促进作用。  相似文献   

高光谱图像波段选择的改进二进制布谷鸟算法     

宋广钦  杜正舜  贺智 《测绘通报》2019,(4):43-48

波段选择是高光谱遥感图像分类的重要前提,本文提出了一种用于高光谱遥感图像波段选择的改进二进制布谷鸟算法,通过使用混合二进制编码算法更新子代鸟巢和使用遗传算法交叉方式更新被发现鸟巢两个方面对二进制布谷鸟算法进行改进,找出在图像中起主要作用且相关性低的波段,实现对高光谱遥感图像降维。将本文算法运用于PaviaU数据集和AVIRIS数据集,并与二进制布谷鸟算法、二进制粒子群算法、最小冗余最大相关算法、Relief算法等进行对比分析。结果表明,改进二进制布谷鸟算法波段特征选择效率更高,且选取的波段更具代表性,能够较好地提高后续分类精度。  相似文献   

地缘政治视角下中亚“颜色革命”易发程度分析     

李希雅  王礼茂 《世界地理研究》2019,28(6):42-49

基于地缘政治视角,分析本世纪初发生在原苏联地区“颜色革命”的诱发因素,认为美国根据其地缘战略格局对“颜色革命”发生地存在一定程度的主观选择性,国家内部经济、政治、社会等层面的矛盾也同样作为发生背景。在中亚地缘格局动态分析的基础上,结合中亚五国形势,从时间和空间双重维度对中亚地区未来发生“颜色革命”的可能性进行识别与评判,得出1) 整体来看,中亚近期内爆发“颜色革命”可能性较小,但从长远来看不能掉以轻心。2)土、乌未来稳定可期,塔、吉、哈需谨防“颜色革命”卷土重来。  相似文献   

黑河流域土地利用变化对土壤有机碳和全氮储量的影响——元分析（英文）     

仝金辉  胡锦华  陆峥  孙浩然  杨晓帆 《地理学报(英文版)》2019,(9):1578-1594

Land use and cover change(LUCC) is an important indicator of the human-earth system under climate/environmental change,which also serves as a key impact factor of carbon balance,and a major source/sink of soil carbon cycles.The Heihe River Basin(HRB) is known as a typical ecologically fragile area in the arid/semi-arid regions of northwestern China,which makes it more sensitive to the LUCC.However,its sensitivity varies in a broad range of controlling factors,such as soil layers,LUCCs and calculation methods(e.g.the fixed depth method,FD,and the equivalent mass method,ESM).In this study,we performed a meta-analysis to assess the response of soil organic carbon(SOC) and total nitrogen(TN) storage to the LUCC as well as method bias based on 383 sets of SOC data and 148 sets of TN data from the HRB.We first evaluated the calculation methods and found that based on the FD method,the LUCC caused SOC and TN storage to decrease by 17.39% and 14.27%,respectively;while the losses estimated using the ESM method were 19.31% and 18.52%,respectively.The deviations between two methods were mainly due to the fact that the FD method ignores the heterogeneity of soil bulk density(BD),which may underestimate the results subsequently.We then analyzed the response of SOC and TN storage to various types of the LUCC.In particular,when woodland and grassland were converted into cultivated land or other land types,SOC and TN suffered from heavy losses,while other LUCCs had minor influences.Finally,we showed that increasing the depth of the soil layers would reduce the losses of SOC and TN storage.In summary,we identified a series of controlling factors(e.g.soil layer,the LUCC and calculation method) to evaluate the impact of the LUCC on SOC and TN storage in the HRB,which should be considered in future research.  相似文献   

Responding to Global Warming: Mitigation Policies and Actions of Stakeholders in China’s Tourism Industry     

FENG Ling  CHEN Da  GAO Shan  WANG Yongzhi  TANG Chengcai 《资源与生态学报(英文版)》2019,10(1):94-103

China’s tourism industry has witnessed rapid progress in recent years, and is now an important part of global tourism in dealing with climate change. Within a framework of Pressure-State-Response (PSR), this paper focuses on the emission reduction pressure, carbon emission status, and responses of stakeholders in China’s tourism industry. Findings include: 1) The central government’s strategy and rapid growth of the industry scale exert rising pressure on China’s tourism to reduce carbon emissions. 2) Carbon emissions of China's tourism account for 13%-14.6% of global tourism, and about 3% of China’s emissions overall. Chinese tourists’ per capita carbon emission is lower than half of the global level. 3) The Chinese government attaches great importance to energy-saving and carbon emission reduction. In the tourism industry, documents, standards and other regulative measures have been issued to ensure that business practitioners set up green operational and managerial systems. In the field of tourism transportation, China's high-speed rail, new energy vehicles, and urban shared bicycles, have developed very rapidly in recent years, and they have effectively reduced the carbon emissions in traveling. Furthermore, this paper finds that Chinese tourists already have awareness and willingness for low-carbon tourism.  相似文献