仿刺参水溶性海参皂苷的分离制备及抗真菌活性的研究   总被引：9，自引：0，他引：9  

丛日山  袁文鹏  樊廷俊  郭瑞超  宣昭  张春阳  蔡慧坤 《中国海洋大学学报(自然科学版)》2006,36(6):959-964

利用海洋生物资源进行天然抗真菌新药的开发,从冻干海参加工废液中提取了水溶性海参皂苷,并对其抗真菌活性进行了研究。采用大孔吸附树脂法提取水溶性海参皂苷,经Libermann Burchard反应等对提取物的性质进行鉴定后,利用管碟法对所得提取物的抗菌活性进行了测定,并利用琼脂稀释法测定了所得水溶性海参皂苷对6株供试真菌的最低抑菌浓度(MIC)。提取物在Molish反应、沉淀反应和泡沫实验中均为阳性,说明提取物中确含有皂苷类成分;在Libermann Burchard反应中,液体的颜色处在红色与紫色之间,显示提取物可能是1种三萜类皂苷。水溶性海参皂苷对6株供试真菌均具有显著的抑制作用,且在浓度为0.5~4 mg/mL的范围内,其抑菌活性与所用浓度之间呈明显的正相关性;在浓度为4 mg/mL时,水溶性海参皂苷对6株供试真菌抑制活性的大小依次为裂殖酵母菌、啤酒酵母菌、白色念珠菌、葡萄炭疽病原菌、黄瓜枯萎病原菌和黑曲霉,海参皂苷对6株真菌的MIC值依次分别为0.002,0.016,0.063,0.063,0.125,0.250 mg/mL。  相似文献   

转炉钢渣对两种对虾幼虾的急性毒性效应     

许刚  宋秀贤  刘云  董晓丹  俞志明 《海洋科学》2007,31(8):76-79

初步研究了一定粒径的转炉钢渣对日本对虾（Penaeus japonicus）幼虾和中国明对虾（fenneropenaeus chinensis）幼虾的急性毒性效应。实验结果显示，当钢渣质量浓度为1.8g／L时，日本对虾幼虾96h的最高死亡率为33．33％；随着钢渣质量浓度的增加，死亡率增高；当钢渣质量浓度为18g／L时，其96h的最大死亡率达到了75％。相对于日本对虾幼虾，钢渣质量浓度为18g／L时中国明对虾幼虾96h的最高死亡率只有36．67％。运用直线内插法求出钢渣对日本对虾幼虾96h半致死浓度为1．62g／L，钢渣对日本对虾的安全浓度为0．162g／L，而在实验条件下中国明对虾幼虾的半致死浓度要明显高于日本对虾幼虾。  相似文献   

台湾海峡中、北部上升流区浮游有孔虫的生态研究     

方惠瑛 《热带海洋学报》1995,(3)

分析１９８７—１９８８年在台湾海峡中、北部用浮游生物网采集的水层中的浮游有孔虫标本，研究调查海区浮游有孔虫的生态分布特征及其与海水温度和盐度的关系。结果表明：该海区浮游有孔虫计有１６种，以泡抱球虫Ｇｌｏｂｉｇｅｒｉｎａｂｕｌｌｏｉｄｅｓ为主要优势种类，其次是袋拟抱球虫Ｇｌｏｂｉｇｅｒｉｎｏｉｄｅｓｓａｃｃｕｌｉｆｅｒ和红拟抱球虫Ｇ．ｒｕｂｅｒ。在夏季的７月和８月（特别是７月），浮游有孔虫密集在福建中部沿岸的南日岛至闽江口外海海域一带沿岸水域，其中９０％以上的个体由泡抱球虫组成。此现象推测与夏季出现在福建沿岸的上升流活动有关。  相似文献   

甲醛对Nessler法和次氯酸酚盐法测定养殖水体总氨氮的影响   总被引：1，自引：0，他引：1  

罗国芝  谭洪新  朱学宝  霍少勇 《中国海洋大学学报(自然科学版)》2002,32(3):397-402

水体中含一定浓度的甲醛 ,用 Nessler法测得的总氨氮 (TAN)浓度远远高于实际浓度 ,差异百分数最高达 4 5 0 .0 6 % ;用次氯酸酚盐法测得的总氨氮 (TAN)浓度则明显低于实际浓度 ,差异百分数最高可达 84 .77%。甲醛浓度范围为 10～ 4 0 mg/ L、TAN浓度为 1～ 10 mg/ L,通过双因素有重复观察值的交叉实验 ,得到如下矫正式 :(1)用 Nessler法测 TAN时 ,Y=4 .0 5 2 9- 0 .0 72 4 x1+ 0 .2 0 6 4x2 ,标准误差为 1.3381;(2 )用次氯酸酚盐法测 TAN时 ,Y=0 .1196 + 0 .0 345 x1+ 1.0 0 2 2 x2 ,标准误差为 0 .4 6 2 7。 Y为 TAN实际浓度 (mg/ L) ,x1为甲醛浓度 (mg/ L) ,x2 为测得的 TAN浓度(mg/ L )。比较含氨氮水体与不含氨氮水体中的甲醛浓度 ,方差分析结果表明 ,没有显著差异。  相似文献   

含沙量对草地坡面径流泥沙沉积和水力特性的影响   总被引：2，自引：0，他引：2       下载免费PDF全文

潘成忠  马岚  上官周平 《水科学进展》2008,19(6):857-862

通过室内模拟试验,在坡度为3°和9°、流量为20和60L/min条件下研究了不同浓度(0～350kg/m3)含沙水流流经草地的泥沙沉积过程及其水力学特性。结果表明,坡面泥沙沉积量随含沙量的增加而增大,3°时泥沙沉积率与含沙量呈正相关,而9°时沉积率与含沙量呈反势。坡度对泥沙沉积影响显著,而在相同坡度条件下,两种流量试验的泥沙沉积量无明显差异。相同坡面坡上部位流速小于坡下部位,且含沙量对坡面流速影响较小。在相同坡度和流量条件下,水流雷诺数随含沙量的增大而减小。3°时水流阻力系数和曼宁糙率均随含沙量的增加而增大,而9°时含沙量对阻力影响不明显,因此在土壤侵蚀较严重地区进行坡面水文过程演算时需考虑含沙量对缓坡糙率的影响。  相似文献   

The Role of Fracture Structure in Leachate Pollution at Landfills： A Case Study of a Landfill in Chongqing City     

LUO Yunju  LIU Dongyan  LI Xiulan  ZHU Zhengwei 《《地质学报》英文版》2008,82(5)

This paper studies a landfill where there are three faults running through.As serious pollution has occurred to the geological environment,the landfill is to be closed up and renovated.The paper aims to explore the role of fracture structure in leachate pollution at the landfill.The research was carried out in several stages.First,mathematical models of the pre-renovation landfill with three faults running through and the landfill after renovation were established.And then,the boundary conditions and parameters of the two mathematical models were determined.The groundwater level of the landfills was simulated in order to modify the two mathematical models.As a result,a feasible mathematical model was achieved.Based on this model,a comparison was made of the COD concentration variations in the inside leachate and outside leachate between the two landfills. Accordingly,the impacts of the fracture structure on the pollution of leachate at the landfills could be identified.The study results show that while faults contribute to the migration of leachate,they also serve as a confluence of leachate,thus further deteriorating the environment.The COD concentrations of the inside leachate and the outside leacbate of the pre-renovation landfill are respectively 800 mg/L and 220 mg/L higher than those of the post.renovation landfill.Therefore,measures must be taken to handle the leachate seepage in areas where there are faults as well as the neighboring areas so as to get the environmental pollution under control.  相似文献   

The Role of Fracture Structure in Leachate Pollution at Landfills: A Case Study of a Landfill in Chongqing City     

LUO Yunju  LIU Dongyan  LI Xiulan  ZHU Zhengwei 《《地质学报》英文版》2008,82(5):1020-1026

This paper studies a landfill where there are three faults running through. As serious pollution has occurred to the geological environment, the landfill is to be closed up and renovated. The paper aims to explore the role of fracture structure in leachate pollution at the landfill. The research was carried out in several stages. First, mathematical models of the pre-renovation landfill with three faults running through and the landfill after renovation were established. And then, the boundary conditions and parameters of the two mathematical models were determined. The groundwater level of the landfills was simulated in order to modify the two mathematical models. As a result, a feasible mathematical model was achieved. Based on this model, a comparison was made of the COD concentration variations in the inside leachate and outside leachate between the two landfills. Accordingly, the impacts of the fracture structure on the pollution of leachate at the landfills could be identified. The study results show that while faults contribute to the migration of ieachate, they also serve as a confluence of leachate, thus further deteriorating the environment. The COD concentrations of the inside leachate and the outside leachate of the pre-renovation landfill are respectively 800 mg/L and 220 mg/L higher than those of the post-renovation landfill. Therefore, measures must be taken to handle the ieachate seepage in areas where there are faults as well as the neighboring areas so as to get the environmental pollution under control.  相似文献   

基于非充分掺混模式的流域来水组成模型          下载免费PDF全文

王船海  朱琰  程文辉  向小华 《水科学进展》2008,19(1):94-98

针对如何减小数值求解对流输运方程耗散误差的问题,引进断面计算浓度的概念来计算河段平均浓度,提出了非充分掺混模式的有限控制体积法离散对流输运方程的新算法,据此构建了模拟流域内的水源组成以及不同水源在流域内的时空变化情况的流域来水组成模型。通过数值试验与具体实例验证,结果表明所提出的非充分掺混模式的新算法可有效地提高流域来水模型的计算精度。  相似文献   

对北秦岭太阳寺矿集区金矿成矿特征的初步认识   总被引：1，自引：0，他引：1       下载免费PDF全文

胡晓隆  赵利利 《甘肃地质》2008,17(1):41-46

甘肃省天水冯家场—两当太阳寺一带,是近年来资源评价和矿产勘查的重点,是秦岭-大别成矿省北秦岭成矿带中重要的矿化集中区。文章在分析区域构造背景的基础上,对该区成矿特征进行了初步总结,选择典型矿床进行了介绍,初次把该区命名为太阳寺矿集区,这将对该区矿产勘查工作部署,深入研究矿集区成矿规律具有参考意义。  相似文献   

云南公路滑坡稳定性分析中模糊信息分析法的应用   总被引：1，自引：0，他引：1  

李瑞娥  谢永利  王家鼎 《工程地质学报》2008,16(1):130-134

将模糊信息分析方法应用于公路滑坡稳定性分析。运用模糊信息分析中的模糊信息信息分配、信息集中等方法,在云南元磨高速公路滑坡的50组公路滑坡资料中选取了滑坡高度、坡度、组成物质的粘聚力、内摩擦角等重要影响因素,建立了其与滑坡稳定性之间的模糊关系,得到相应的滑坡稳定性分析数学模型。应用该模型对该区域其他滑坡进行验证,其效果良好,故该方法可较好的应用于实际。  相似文献