Development and characterization of a cell line from the embryos of half smooth tongue sole (Cynoglossus semilaevis)   总被引：3，自引：1，他引：2  

SHA Zhenxi  REN Guocheng  WANG Xianli  WANG N  CHEN Songlin 《海洋学报(英文版)》2010,29(2):81-87

A new cell line,CSEC,has been successfully established from embryos at gastrula stage of a cultured marine fish,half smooth tongue sole(Cynoglossus semilaevis).CSEC cells grow actively and stably more than 50 passages for over 200 d in DMEM medium supplemented with 15% FBS(fetal bovine serum),2.5 ng/cm 3 bFGF(basic fibroblast growth factor),1 ng/cm 3 LIF(leukemia inhibitory factor) and 50 mmol/dm 3 2-ME(2-mecaptoethanol).The cells grew well in the temperature range of 24-30 ℃ and the optimal growth temperature was 24℃.FBS and bFGF concentrations are the two key components for CSEC cells proliferation.Chromosome analysis reveals that CSEC cells have a normal diploid karyotype with 2n=42t.The significant fluorescent signals were observed in CSEC after transfection with the GFP reporter gene,suggesting that the CSEC cell line can be used as a useful tool for transgenic and genetic manipulation studies.CSEC cells showed the cytopathic effect(CPE) after infection with lymphosystis disease virus(LCDV) in 2 d.Moreover,the LCDV particles can be observed in the cytoplasm of virus-infected cells by electron microscopy.It suggests that CSEC could be potentially used for the study of aquatic virus.  相似文献   

Stable ⁸⁵Rb micro vapour cells: fabrication based on anodic bonding and application in chip-scale atomic clocks     

苏娟  邓科  郭等柱  汪中  陈兢  张耿民  陈徐宗 《海洋学报(英文版)》2010,29(11)

We describe the microfabrication of ⁸⁵Rb vapour cells using a glass-silicon anodic bonding technique and in situ chemical reaction between rubidium chloride and barium azide to produce Rb. Under controlled conditions, the pure metallic Rb drops and buffer gases were obtained in the cells with a few mm³ internal volumes during the cell sealing process. At an ambient temperature of 90 ℃ the optical absorption resonance of ⁸⁵Rb D1 transition with proper broadening and the corresponding coherent population trapping (CPT) resonance, with a signal contrast of 1.5% and linewidth of about 1.7 kHz, have been detected. The sealing quality and the stability of the cells have also been demonstrated experimentally by using the helium leaking detection and the after-9-month optoelectronics measurement which shows a similar CPT signal as its original status. In addition, the physics package of chip-scale atomic clock (CSAC) based on the cell was realized. The measured frequency stability of the physics package can reach to 2.1×10^-10 at one second when the cell was heated to 100 ℃ which proved that the cell has the quality to be used in portable and battery-operated devices.  相似文献   

陕西金堆城花岗岩类主要造岩矿物特征及其岩石学意义          下载免费PDF全文

聂凤军 《岩石矿物学杂志》1988,7(4):337-347

本文依据大量测试数据,对金堆城地区花岗岩类主要造岩矿物学特征同钼迁移富集成矿关系进行了探讨。研究结果表明:异常晶胞条纹长石和正长石以及富重稀土和具明显负铕异常的黑云母是含钼花岗岩类标型矿物,并且可作为花岗岩类岩石发育地区寻找隐伏钼矿体的指示性矿物。钾钠长石和斜长石的结构状态和成分不仅可以提供有关花岗岩类成因演化的信息,其矿物本身亦可成为钼的载体矿物。岩浆期后,强烈的热液蚀变作用可造成钾钠长石和斜长石分解,并且引起钼的释放,从而形成高品位钼矿石。  相似文献   

自然金—银类质同象系列矿物晶胞参数变化规律及其表述方程     

姜跃先 《矿物学报》1993,13(3):250-253

本文提出了自然金-银类质同象系列矿物的晶胞参数与金、银原子数百分比成二次函数关系,推导出了表示二者之间函数关系的方程式。利用该方程式,可以用金原子数百分比计算出晶胞参数,反之亦然。并且从金、银二者的电负性出发,对a值的变化作了解释。  相似文献   

新疆萨尔布拉克金矿找矿矿物学标型特征研究   总被引：2，自引：1，他引：2  

吕瑞英 《地球科学》1990,15(6):657-665

  相似文献   

攀西地区长石的X射线研究     

Shu Jinfu 《地球科学》1986,(6)

本文对四川攀枝花—西昌（即攀西裂谷）地区各个不同时期、不同产状、不同岩类中的斜长石、碱性长石进行了X射线研究,配合单矿物化学分析、电子探针成分分析,结合红外光谱、光学测定,精确地计算了42个斜长石,60个碱性长石的晶胞参数,求出其四个四面体的Al含量,确定长石的成分和结构态。为探索攀西地区岩浆活动演化规律,研究攀西裂谷形成、发展及其消亡历史提供了客观依据。  相似文献   

新疆库布苏金矿石英标型特征研究   总被引：2，自引：0，他引：2       下载免费PDF全文

吕瑞英 《地球科学》1995,20(1):53-57

重点研究了库布苏矿化带中石英脉与金矿化关系，对不同成矿阶段石英的化学成分，晶胞参，红外光谱，热发光，包裹体等进行了研究，总结了找矿矿物学标志。  相似文献   

BP网络在位场解释中的应用     

朱晓军  申宁华 《吉林大学学报(地球科学版)》1995,(1)

介绍了ＢＰ网络的特点，阐述了它在位场资料解释中应用的方法、特点和可行性。通过模型计算证实了该方法在位场异常识别中的效果，正确率达９７％。此外，通过修改网络单元的激发函数，增强网络的稳定性和内插功能，使ＢＰ网络在多层密度界面反演中得到满意的结果，模型和实例计算证明了该方法的有效性。  相似文献   

基于GIS的单元簇遗传建模及其在区域矿产预测中的应用     

张振飞  高凤亮  马智民  姬金生 《地球科学与环境学报》2001,23(3):15-19

论述一种GIS支持下的基于"单元簇”概念和遗传建模的多源地学信息综合分析方法及在多目标矿产预测中的应用.在以往矿产定量预测中,根据随机抽样思想进行单元划分,对空间信息利用不够充分.用单元的空间组合("单元簇”)代替单元作为定量类比的基本单位,从而能较好实现地质变量值及其所反映的局部地质空间结构的定量类比;将单元作为GIS区图元,利用GIS空间分析功能实现对单元及单元簇的管理和操作;以已知样品判对率最大为适应值计算准则,用遗传算法建立线性判别函数,可快速有效地实现多目标矿产定位预测.以新疆康古尔塔格地区金矿预测实例说明了其应用效果.  相似文献   

Fairweather atmospheric electricity at Antarctica during local summer as observed from Indian station,Maitri     

C. Panneerselvam  C. Selvaraj  K. Jeeva  K. U. Nair  C. P. Anilkumar  S. Gurubaran 《Journal of Earth System Science》2007,116(3):179-186

Surface measurements of the atmospheric electrical parameters like Maxwell current, electric field and conductivity studied at the Indian station, Maitri (70.75° S, 11.75° E, 117 m above mean sea level), Antarctica, during austral summer have been analyzed for the years 2001 to 2004. A total of 69 days were selected which satisfied the ‘fairweather’ conditions, i.e., days with absence of high winds, drifting or falling snow, clouds, and fog effects. The diurnal variation curve of electric field and vertical current averaged for 69 fairweather days is a single periodic with a minimum at 03:00 UT and a maximum near 19:00 UT, which is very similar to the Carnegie curve. The correlation coefficient between these measured parameters has a high value (more than 0.9) for all the days. During fairweather days the measured current and field variations are similar and hence it is clear that the conductivity is more or less stable. During magnetically disturbed days, the dawn-dusk potential drop has clear influences on the diurnal variation and it modifies the conductivity. Apart from the day-to-day variation in low latitude thunderstorm activity, there are diurnal, seasonal, inter-annual variations in the electric potential and the currents, as well as solar influences on the measured parameters. This study will help us to examine the impact of solar and geophysical phenomena like solar flares, geomagnetic storms and substorms on the global electric circuit.  相似文献