Application of Re-Os Geochemistry to Sedimentary Basin: Stratigraphic Correlation, Oil - Source Correlation and Paleo-Environment Condition     

XU Guangping  Judith L. Hannah  Holly J. Stein  Svetoslav Georgiev 《《地质学报》英文版》2013,87(Z1):602-604

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不同温度下脉冲式磷酸盐输入对裸甲藻生长的影响     

牛海凤  冯剑丰  周慧敏  朱琳 《海洋科学》2014,38(5):31-38

脉冲式输入营养盐是陆源向海洋输入营养盐的一种重要方式。本文在实验室条件下,模拟脉冲式磷酸盐输入的方法,研究不同温度下磷酸盐脉冲输入对裸甲藻(Gymnodinium sp.)生长的影响。结果发现:10℃低温条件下,低质量浓度磷酸盐脉冲试验中,每天输入一次的脉冲方式下裸甲藻的长势在一段时间段内占据优势,但整体来看,差异并不显著;高质量浓度磷酸盐脉冲试验中,每8天输入一次的脉冲方式下藻的长势占据明显优势,差异显著(P0.05)。而在25℃常温条件下,无论是低质量浓度脉冲组还是高质量浓度脉冲组,基本表现为1天输入一次的脉冲方式下的裸甲藻藻密度都占有较明显优势(低质量浓度组P0.01;高质量浓度组P0.01)。不管在低温或常温下,在所有实验组中,脉冲频率对裸甲藻的影响基本表现为:在中等频率的磷酸盐脉冲下,藻密度最低。  相似文献   

Nutrient-controlled growth of Skeletonema costatum: an applied model   总被引：1，自引：0，他引：1  

孙科  丘仲锋  何宜军  尹宝树 《中国海洋湖沼学报》2014,32(3):608-625

To model Skeletonema costatum blooms and their relationship with environmental parameters in situ, a S. costatum-specifi c zero-dimensional box model based on the mechanistic model Eco3M was established using physiological features. The parameters were calibrated using experimental counterparts, and simulations were compared with published laboratory fi ndings. The resulting normalized objective function(NOF) values are less than 1.0(and in most cases less than 0.58) and the values for the slope γ(between 0.656 7–1.127 4) and R 2(between 0.806 8–0.971) are close to 1.0 for most of the sub-fi gures. This indicates good agreement between simulated and measured data and suggests that the model reproduces the general characteristics of S. costatum growth and use of nutrients under different N- or P-limiting conditions. The model is appropriate for further applications and can be used to test more scenarios using other nutrients.  相似文献   

A microsatellite genetic linkage map of black rockfish (Sebastes schlegeli)     

Guannan Chu  Liming Jiang  Yan He  Haiyang Yu  Zhigang Wang  Haibin Jiang  Quanqi Zhang 《中国海洋大学学报(英文版)》2014,13(6):1078-1086

Ovoviviparous black rockfish (Sebastes schlegeli) is an important marine fish species for aquaculture and fisheries in China. Genetic information of this species is scarce because of the lack of microsatellite markers. In this study, a large number of microsatellite markers of black rockfish were isolated by constructing microsatellite-enriched libraries. Female- and male-specific genetic linkage maps were constructed using 435 microsatellite markers genotyped in a full-sib family of the fish species. The female linkage map contained 140 microsatellite markers, in which 23 linkage groups had a total genetic length of 1334.1 cM and average inter-marker space of 13.3 cM. The male linkage map contained 156 microsatellite markers, in which 25 linkage groups had a total genetic length of 1359.6 cM and average inter-marker distance of 12.4 cM. The genome coverage of the female and male linkage maps was 68.6% and 69.3%, respectively. The female-to-male ratio of the recombination rate was approximately 1.07:1 in adjacent microsatellite markers. This paper presents the first genetic linkage map of microsatellites in black rockfish. The collection of polymorphic markers and sex-specific linkage maps of black rockfish could be useful for further investigations on parental assignment, population genetics, quantitative trait loci mapping, and marker-assisted selection in related breeding programs.  相似文献   

HPLC pigment profiles of 31 harmful algal bloom species isolated from the coastal sea areas of China     

Shuxia Liu  Peng Yao  Zhigang Yu  Dong Li  Chunmei Deng  Yu Zhen 《中国海洋大学学报(英文版)》2014,13(6):941-950

Chemotaxonomy based on diagnostic pigments is now a routine tool for macroscopic determination of the composition and abundance of phytoplankton in various aquatic environments. Since the taxonomic capability of this method depends on the relationships between diagnostic pigments and chlorophyll a of classified groups, it is critical to calibrate it by using pigment relationships obtained from representative and/or dominant species local to targeted investigation area. In this study, pigment profiles of 31 harmful algal bloom(HAB) species isolated from the coastal sea areas of China were analyzed with high performance liquid chromatography(HPLC). Pigment compositions, cellular pigment densities and ratios of pigments to chlorophyll a were determined and calculated. Among all these species, 25 kinds of pigments were detected, of which fucoxanthin, peridinin, 19'-butanoyloxyfucoxanthin, 19'-hexanoyloxyfucoxanthin, violaxanthin, and antheraxanthin were diagnostic pigments. Cellular pigment density was basically independent of species and environmental conditions, and therefore was recommended as a bridge to compare the results of HPLC-CHEMTAX technique with the traditional microscopy method. Pigment ratios of algal species isolated from the coast of China, especially the diagnostic pigment ratios, were higher than those from other locations. According to these results, pigment ratio ranges of four classes of phytoplankton common off the coast of China were summarized for using in the current chemotaxonomic method. Moreover, the differences of pigments ratios among different species under the same culturing conditions were consistent with their biological differences. Such differences have the potential to be used to classify the phytoplankton below class, which is meaningful for monitoring HABs by HPLC-CHEMTAX.  相似文献   

吉林省黑土区土壤--作物系统Cd元素转化积累研究     

杨小琳  王冬艳  姜琦刚  李月芬  付强 《世界地质(英文版)》2014,(3)

在吉林省黑土区系统的野外调查和土壤及玉米籽实样品采集、测试的基础上,采用地球化学分析方法及SPSS技术,研究了Cd元素的土壤地球化学特征,及其在土壤和作物籽实中的含量特征和相关性,用以揭示Cd元素在土壤-作物系统中的转化累积特征及影响因素,为粮食安全服务。研究表明：吉林省黑土区Cd元素全量高于吉林省及全国土壤背景值,但不存在污染,籽实中Cd含量低于国家安全标准。土壤中Cd元素有效态转化程度较高,受其全量控制,同时Zn、 Cu及P元素有效量对Cd转化的作用明显,偏酸性土壤更利于Cd元素的转化。玉米籽实对Cd吸收程度较低,主要取决于其自身的生物特性, Zn元素缺乏可能导致玉米籽实对Cd元素吸收增加。  相似文献   

中华乌塘鳢(Bostrychus sinensis) GPx7和GPx8基因的克隆、表达与酶活性分析     

丁浪  夏立萍  沈斌  张建设 《海洋与湖沼》2022,53(3):697-709

中华乌塘鳢(Bostrychus sinensis)属于广盐广温、低氧耐受性强、药用价值高的鱼类,为我国东南沿海重要养殖对象。谷胱甘肽过氧化物酶(glutathione peroxidase,GPx)是生物体内重要的抗氧化酶,能够清除过氧化氢防止机体生物膜氧化受损,研究中华乌塘鳢GPx基因对其养殖及海洋生态研究具有重要意义。利用分子克隆技术获得了中华乌塘鳢GPx7和GPx8(分别命名为BsGPx7和BsGPx8)的cDNA序列。BsGPx7编码186个氨基酸,含有2个半胱氨酸(Cys⁵⁶和Cys⁸⁵)催化位点;BsGPx8编码210个氨基酸,含有1个半胱氨酸(Cys¹⁰⁹)催化位点。利用RT-PCR方法研究了BsGPx7、BsGPx8基因在中华乌塘鳢组织中的分布表达,以及不同浓度镉离子(7.5,15,30 mg/L)胁迫下BsGPx7、BsGPx8基因的表达量变化。结果显示,BsGPx7和BsGPx8在组织中呈泛在性表达,其中肝脏的表达量最高,其次是鳃和皮肤。不同浓度的镉离子胁迫下,BsGPx7基因在鳃和皮肤中的表达量均显著上调(P<0.05),BsGPx8基因在肝脏、鳃和皮肤中的表达量均显著上调(P<0.05)。成功构建了原核表达载体,表达并纯化获得了BsGPx7重组蛋白(rBsGPx7)。对rBsGPx7在不同温度和pH条件下的酶活性进行了测定,表明rBsGPx7在35℃和pH 8条件下酶活性最高。综上提示,BsGPx7与BsGPx8参与了机体对抗镉离子诱导的氧化应激的过程,可能在抗氧化防御中起一定作用。  相似文献   

Spatial and diel variations of the prokaryotic community in the Phaeocystis globosa blooms area of Beibu Gulf,China     

Cheng He  Sha Xu  Shuqun Song  Caiwen Li 《海洋学报(英文版)》2022,41(12):87-97

While prokaryotes play key roles in nutrient cycling and energy flow during Phaeocystis globosa blooms, the information on the spatial and diel temporal distribution of the prokaryotic community during Phaeocystis blooms remains scarce. In January 2019, we used high-throughput sequencing of the 16S rRNA gene to explore the spatial and diel variations of particle-attached (PA) and free-living (FL) prokaryotic communities during the blooming phase of P. globosa in Beibu Gulf, Guangxi, China. The results suggested a significant spatial variation pattern in the horizontal distribution of prokaryotic communities, while there was no significant difference in the vertical direction. Both spatial distance and environmental variables shaped the horizontal distribution of the prokaryotic community structure, while environmental variables, particularly the abundance of P. globosa colony and Chl a, showed more significant influence and were closely related to the structure and variation of the prokaryotic community. Strong vertical mixing of the water column disrupted the vertical structure heterogeneity of the prokaryotic community in winter. There were significant differences in the diel samples of PA prokaryotic communities, but not in the FL prokaryotic communities. Nitrate, ammonium and the abundance of P. globosa colony were the key environmental variables impacting the diel variations of prokaryotic communities over the sampling period. The present study provided valuable information to depict the spatial-temporal variations of the microbial community and its association with environmental parameters during P. globosa bloom in the tropical gulf.  相似文献   

基于最大熵和广义相加模型分析厦门有害藻华影响下滨海旅游环境价值损失的关键参数     

翁鸣鸣  王昌运  柳欣 《海洋通报》2022,(5)

有害藻华是海洋生态灾害中最突出的问题,威胁滨海旅游等海洋经济可持续发展。如何有效评估有害藻华对滨海旅游环境价值损失的影响,是推动生态环境保护与旅游资源开发协调发展的前提和基础。目前国内外广泛使用条件价值法,通过设置假想情景,根据受访者是否愿意为修复环境支付费用及其多少来定量转换环境损失价值。因此,受访者支付意愿及其程度是影响环境损失价值的关键参数。但是,受访者的支付意愿和最大支付额度受多种复杂因素影响,常规统计分析方法难以厘清其主要因素。本文创新性引入最大熵MaxEnt和广义相加GAMs模型,分别从是否愿意支付的定性分析和支付额度多少的定量分析两个方面,阐明厦门有害藻华影响下滨海旅游环境价值损失评估中影响这两个关键参数的主要因素。与传统统计分析方法相似,不论是MaxEnt还是GAMs模型都认为收入水平、对景点满意程度、受教育程度和年龄是解释支付意愿和支付额度的主要影响因素。但是,模型分析结果给出的显著性和重要性排序发现游客对有害藻华的科学认识确实会显著影响其支付意愿,但是对支付额度的影响有限。本文通过研究受访者对生态灾害的环境态度及其对支付意愿和支付额度的影响,为准确评估海洋生态灾害造成的环境价值损失提供科学依据。  相似文献   

The role of metropolitan opportunity structures for understanding variation in the rate of Black household affluence     

Ron Malega 《Urban geography》2013,34(4):530-549

This study explores Black household affluence at the metropolitan scale and suggests that metropolitan-level opportunity structures shaped rates of Black affluence for the 100 largest American metropolitan areas in 2000. I hypothesize that affluent black households favored metropolitan areas of opportunity, those places characterized by having (1) economic opportunities, (2) favorable Black–White relational standing, (3) metropolitan diversity and residential opportunities, and (4) their location in the South, which serves as a Black homeplace. Results fail to suggest evidence regarding the role of the ‘new South’ for understanding metropolitan-level rates of Black affluence. More generally, findings from this study challenge our understanding of socioeconomic stratification by investigating diversity within America’s Black community.  相似文献