Development of 101 Novel EST-Derived Single Nucleotide Polymor- phism Markers for Zhikong Scallop （Chlamysfarren）     

LI Jiqin  BAO Zhenmin  LI Ling  WANG Xiaojian  WANG Shi  HU Xiaoli 《青岛海洋大学学报(英文版)》2013,(3):403-412

Zhikong scallop （Chlamys farreri） is an important maricultured species in China. Many researches on this species, such as population genetics and QTL fine-mapping, need a large number of molecular markers. In this study, based on the ex- pressed sequence tags （EST）, a total of 300 putative single nucleotide polymorphisms （SNPs） were selected and validated using high resolution melting （HRM） technology with unlabeled probe. Of them, 101 （33.7%） were found to be polymorphic in 48 indi- viduals from 4 populations. Further evaluation with 48 individuals from Qingdao population showed that all the polymorphic loci had two alleles with the minor allele frequency ranged from 0.046 to 0.500. The observed and expected heterozygosities ranged from 0.000 to 0.925 and from 0.089 to 0.505, respectively. Fifteen loci deviated significantly from Hardy-Weinberg equilibrium and significant linkage disequilibrate was detected in one pair of markers. BLASTx gave significant hits for 72 of the 101 poly- morphic SNP- containing ESTs. Thirty four polymorphic SNP loci were predicted to be non-synonymous substitutions as they caused either the change of codons （33 SNPs） or pretermination of translation （1 SNP）. The markers developed can be used for the population studies and genetic improvement on Zhikong scallop.  相似文献   

鲢序列已知标记连锁图构建及鲢-草鱼标记共线性分析     

杨静  张鹏  危起伟  邹桂伟  杨官品 《中国海洋大学学报(自然科学版)》2012,(Z2):76-81

鲢、草鱼是传统池塘养殖的四大家鱼的2个重要成员,其遗传资源评估与管理、性状遗传基础研究、功能基因从头克隆等迫切需要序列已知标记连锁图谱工具。本研究将已有鲢连锁图谱新整合53个草鱼微卫星DNA标记和28个鲢InDel-RFLP标记,构建了鲢已知序列标记连锁图谱,标记数326个,达到中等密度水平。构建的图谱将为鲢、鳙资源评价和管理、性状遗传基础研究等提供工具。另外,本研究初步比较了鲢、草鱼间标记共线性排列,发现两物种染色体既存在共线性,也存在明显重排。  相似文献   

基于组件模型技术的地磁数据干扰抑制系统     

谢凡  滕云田  徐沁 《地震地磁观测与研究》2012,33(2):152-158

地磁观测数据中的人工干扰抑制等预处理工作需要一套高效、便捷、交互性好的预处理系统.将基于Matlab的核心干扰识别和抑制算法以组件对象技术的形式提供给其他编程语言调用,在系统架构上采用Windows DNA架构,在界面的开发上采用微软成熟的MFC架构.同时,给出系统架构模型及关键技术实现.该系统在实际应用中取得良好效果.  相似文献   

Sensitive and rapid detection of two toxic microalgae Alexandrium by loop-mediated isothermal amplification     

ZHANG Fengying  SHI Yanhong  JIANG Keji  XU Zhaoli  MA Lingbo 《海洋学报(英文版)》2012,31(2):139-146

A loop-mediated isothermal amplification (LAMP) assay was designed and evaluated for rapid detection of the toxic microalgae Alexandrium catenella and A.minutum,which can produce paralytic shellfish poisoning (PSP).Two sets of four specific primers targeting these two species were derived from the sequence of internal transcribed spacer (ITS) of ribosomal DNA.The method worked well in less than an hour under isothermal conditions of 65 C.LAMP specificity was validated in closely related algae as a comparison,suggesting the strict specificity of the LAMP primers.Two visual inspection approaches were feasible to interpret the positive or negative results.The detection limits of A.catenella and A.minutum samples using the LAMP assay were found to be 5.6 and 4.5 pg DNA,respectively.The sensitivity of this LAMP assay was 10 or 100-fold higher than Polymerase Chain Reaction (PCR) method in detecting the two microalgae.These characteristics of species specificity,sensitivity,and rapidity suggest that this method has the potentiality in the monitoring of red tide caused by A.catenella and A.minutum.  相似文献   

哲罗鱼(Hucho taimen)染色体组型与DNA含量分析   总被引：1，自引：0，他引：1  

薛淑群  尹洪滨  尹家胜 《广东海洋大学学报》2010,30(3):6-10

采用体内注射PHA和秋水仙素、肾细胞短期培养、常规空气干燥法制备哲罗鱼(Hucho taimen)的染色体。对其肾细胞染色体数目统计分析表明,哲罗鱼染色体组有84条染色体,核型公式为2n=18m+16sm+34st+16t,其染色体总臂数(NF)为118。采用流式细胞分析仪测定哲罗鱼的DNA含量,与鸡血细胞标准对照的比值为2.23±0.17,以鸡红细胞DNA含量2.30pg·N~(-1)计,则哲罗鱼的体细胞DNA含量为5.12pg·N~(-1)。哲罗鱼染色体数目和DNA含量体现为四倍体特征。  相似文献   

军曹鱼全人工繁殖群体遗传特征的SSR分析   总被引：2，自引：1，他引：1  

王中铎  陈铁妹  郭昱嵩  罗杰  刘丽  刘楚吾 《广东海洋大学学报》2010,30(3):16-21

利用8个微卫星DNA位点分析南海海域5个军曹鱼全人工繁育群体(HN1、HN2、ZJ、FJ和LS)子代的遗传多样性特征和群体间遗传分化。结果显示,军曹鱼养殖群体与天然群体的遗传结构特征基本一致:1)平均有效等位基因数为3.910±0.440,观测杂合度为0.595±0.049,分子方差分析(AMOVA)表明,个体内分化占主导(46%),军曹鱼养殖群体整体遗传多样性较高;2)群体间基因流明显(N_m=2.5959,F_(st)=0.0878),整体分化程度较低。各养殖群体表现出不同于天然大群体的特征:1)绝大部分位点均明显偏离哈温平衡,杂合子缺失或过剩现象普遍存在;2)聚类和群体分配分析等表明HN2与另四个养殖群体(HN1、ZJ、FJ和LS)分化明显。  相似文献   

一种提取高质量贝类基因组DNA的简易方法     

彭敏  蒋伟明  陈秀荔  杨春玲  李咏梅 《广东海洋大学学报》2010,30(6)

以企鹅珍珠贝(Pteria penguin)闭壳肌为样品,利用高盐+十二烷基硫酸钠(SDS)除去蛋白和多糖,用低盐+十六烷基三甲基溴化铵(CTAB)进一步纯化DNA,建立一种贝类基因组DNA简便、安全、经济的提取方法。改进后的SDS-CTAB法提取的DNA纯度较高,无蛋白质和RNA污染;酶切和AFLP实验验证表明,所提取DNA可满足酶切分析和分子标记等实验的要求。  相似文献   

三种笛鲷属鱼类的随机扩增多态性DNA初步研究     

易乐飞  刘楚吾 《广东海洋大学学报》2001,21(1):70-72

RAPD技术是由 Williams[1]和 Welsh[2 ]领导的两个科研小组于 1 990年几乎同时独立创立的一种 DNA多态性分析技术 ,它的原理是利用一系列随机排列的寡核苷酸单链引物 ,以研究对象的基因组 DNA为模板进行 PCR扩增 ,通过扩增产物 DNA片段的多态性来检测基因组 DNA的多态性。该技术已被广泛地应用于种质鉴定、遗传多样性、亲缘关系及系统分类研究 [3～ 6]。但对海水鱼类RAPD研究报道较少。红鳍笛鲷 (L utjanus erythopterus Bloch)、紫红笛鲷 (L utjanus argentimaculatus Forskal)和勒氏笛鲷 (L utjanus russelli Bleeker)具有…  相似文献   

三种笛鲷属鱼类的随机扩增多态性DNA初步研究   总被引：1，自引：0，他引：1  

易乐飞  刘楚吾 《广东海洋大学学报》2001,21(1):70-72

RAPD技术是由Williams和Welsh领导的两个科研小组于1990年几乎同时独立创立的一种DNA多态性分析技术，它的原理是利用一系列随机排列的寡核苷酸单链引物，以研究对象的基因组DNA为模板进行PCR扩增，通过扩增产物DNA片段的多态性来检测基因组DNA的多态性。  相似文献   

Sequence Comparison of Partial Cytochrome b Genes of Two Coilia species   总被引：2，自引：0，他引：2  

LIU Jinxian)  )  GAO Tianxiang)  WANG Yujiang)  and ZHANG Yaping) ) The Key Laboratory of Mariculture  Ministry of Education  Ocean University of China  Qingdao  P.R.China ) Laboratory of Molecular Evolution  Genome Diversity  Kunming Institute of Zoology  Chinese Academy of Sciences  Kunming  P. R. China 《中国海洋大学学报(英文版)》2005,4(1):85-88

1Introduction Japanese grenadier anchovy,Coilia ectenes Jordanet Seale(1905),is distributed in the northwest Pacif-ic.The congener,Osbeck's grenadier anchovy,Coil-ia mystus(Linnaeus,1758),is widely distributed inthe west Pacific.Both species inhabit coastal watersand estuaries and can reach up to rivers.The twospecies are important contributors to the coastal fish-eries of China(Zheng et al.,2003).Many studies on the fishery biology of the twospecies have been conducted(Zheng et al.,2003)…  相似文献