Molecular evidence for Myripristis jacobus and Scarus taeniopterus new to Bay of Bengal: Sporadic appearance or preliminary colonization?     

Tapan K. Barik  Surya N. Swain  Bijayalaxmi Sahu  Bibarani Tripathy  Usha R. Acharya 《Marine Ecology》2021,42(1):e12632

Individual specimens of Myripristis jacobus Cuvier, 1829 and Scarus taeniopterus Lesson, 1829 were collected from Gopalpur‐on‐sea, Odisha coast, Bay of Bengal. Both the fish samples were assigned species level tags using the conventional taxonomic methods. The capture of both the fish species is of particular interest in that they were new to the Bay of Bengal. Molecular analysis using the mitochondrial cytochrome oxidase subunit I (COI) gene showed high confidence sequence similarity in species identification. The resultant phylogenetic relationships strongly support the monophyly of both the genus with congruent clustering of both the species according to their morphological identification. In addition, the time tree produced is in complete agreement with the recent studies. These studies conclusively prove that both the species might have been originated during the middle to the late Miocene period. Furthermore, both fish species are typical inhabitants of stigmatized coral reefs, confined to the reef regions. These fish species perhaps migrated to the Bengal Bay from the Andaman and Nicobar Islands reef regions because of climatic changes.  相似文献   

马氏珠母贝(Pinctada martensii)四种壳色选育系F₅ 的生长及遗传多样性分析     

朱晓闻  刘志刚  王辉  邬思荣 《海洋与湖沼》2012,43(1):160-165

采用跟踪测量和微卫星(SSR)技术研究了马氏珠母贝四种壳色选育系F5和对照组的存活率、生长情况及遗传多样性。结果表明,四种壳色选育系和对照组的生长性状之间均存在显著差异(P<0.05)。从30对微卫星引物中扩增筛选获得8个多态位点,多态位点比例为26.67%,它们在4个壳色选育系共120个个体中产生了42个等位基因,平均每个多态位点产生5.25个。4个选育系的平均期望杂合度范围为0.6622—0.6850,平均观察杂合度范围为0.2708—0.4667,平均多态信息含量PIC值范围为0.6025—0.6190,说明4个选育系的遗传多样性处于较高水平,具有育种潜力;平均遗传偏离指数均为负值,4个选育系均存在不同程度的杂合子缺失。遗传分化和遗传距离分析表明白壳色选育系与红壳色选育系之间的亲缘关系最近,黑壳色与白壳色之间的遗传距离最大。  相似文献   

Applications of three DNA barcodes in assorting intertidal red macroalgal flora in Qingdao, China     

ZHAO Xiaobo  PANG Shaojun  SHAN Tifeng  LIU Feng 《中国海洋大学学报(英文版)》2013,12(1):139-145

This study is part of the endeavor to construct a comprehensive DNA barcoding database for common seaweeds in China. Identifications of red seaweeds, which have simple morphology and anatomy, are sometimes difficult solely depending on morphological characteristics. In recent years, DNA barcode technique has become a more and more effective tool to help solve some of the taxonomic difficulties. Some DNA markers such as COI (cytochrome oxidase subunitⅠ) are proposed as standardized DNA barcodes for all seaweed species. In this study, COI, UPA (universal plastid amplicon, domain V of 23S rRNA), and ITS (nuclear internal transcribed spacer) were employed to analyze common species of intertidal red seaweeds in Qingdao (119.3 -121 E, 35.35 -37.09 N). The applicability of using one or a few combined barcodes to identify red seaweed species was tested. The results indicated that COI is a sensitive marker at species level. However, not all the tested species gave PCR amplification products due to lack of the universal primers. The second barcode UPA had effective universal primers but needed to be tested for the effectiveness of resolving closely related species. More than one ITS sequence types were found in some species in this investigation, which might lead to confusion in further analysis. Therefore ITS sequence is not recommended as a universal barcode for seaweeds identification.  相似文献   

A preliminary phylogenetic analysis of Luidia (Paxillosida: Luidiidae) from Chinese waters with cytochrome oxidase subunit I (COI) sequences     

XIAO Ning  LIU Ruiyu  YUAN Shuai  SHA Zhongli 《中国海洋大学学报(英文版)》2013,12(3):459-468

Luidia Forbes (Paxillosida: Luidiidae) are common soft bottom sea stars with 49 described species. Because of substantial morphological diversity, the taxonomy of the genus is complex and hasn’t been resolved definitely. In order to resolve general taxonomic issues, and determine species boundaries and phylogenetic relationships within the genus Luidia, the sequences of cytochrome oxidase subunit I (COI) gene from 24 specimens of Luidia, belonging to eight taxa in Chinese waters, were studied. Three sequences of two species in genus Luidia from GenBank were used to analyze the phylogenetic relationships. The molecular phylogeny exhibited three main clades, each with strong bootstrap support: Clade A including Luidia quinaria from the Sea of Japan; Clade B including seven nominal species (L. quinaria von Martens, L. yesoensis Goto, L. changi Liu, Liao and Li, L. orientalis Fisher, L. avicularia Fisher, L. longispina Sladen and L. hardwicki Gray) from Chinese waters; and Clade C including L. maculata Müller & Troschel from Chinese waters. Our molecular phylogeny results support the morphological Quinaria-Group and Alternata-Group assigned by Döderlein. Seven nominal species we sampled do not exhibit genetic distances that are large enough to recognize them as separate species. Cryptic species may exist in’ Luidia quinaria’ from the Yellow Sea and the Sea of Japan. Meaningful morphological characters need further investigation in Luidia.  相似文献   

两种扇贝杂交和自交家系早期生长及甲基化的比较分析   总被引：1，自引：0，他引：1  

吴彪  杨爱国  刘志鸿  周丽青  程鹏  于涛 《海洋科学》2012,36(2):1-6

运用杂交和自交方法建立虾夷扇贝♂(A)×栉孔扇贝♀(B)、栉孔扇贝♀(B)×栉孔扇贝♂(C)、栉孔扇贝♂(C)×栉孔扇贝♀(D)3 种组合家系, 每个组合3 个平行, 共9 个家系。对各家系卵径大小、胚胎孵化率、幼虫浮游阶段壳长生长速度等生物学参数进行比较, 建立了幼虫浮游期壳长与日龄的线性回归方程; 运用甲基化敏感扩增多态性(MSAP)分析了基因组DNA 胞嘧啶甲基化水平, SPSS 分析了DNA 甲基化与壳长生长的相关性。结果表明, 杂交家系的卵径大小、胚胎孵化率与自交组没有显著差异, 但壳长生长速度显著高于自交组, 这种优势可能主要来自于父本的影响; 家系AB、BC、CD 的平均DNA甲基化率分别为18.672%、22.661%和22.303%, 杂交使后代DNA 甲基化水平降低, 生长速度与DNA甲基化水平相关系数为?0.934, 相关水平极显著(P<0.01), DNA 甲基化与杂种优势具有一定的关系。  相似文献   

东海原甲藻核酸和蛋白对环境中氮磷浓度变化的响应     

刘云  宋秀贤  曹西华  袁涌铨  俞志明 《海洋学报》2012,34(2):153-161

通过设定不同的氮(N)、磷(P)浓度一次性培养东海原甲藻,研究了该藻的生长状况及不同生长阶段单位细胞DNA,RNA及蛋白质含量的变化,结果表明,不同氮磷浓度对东海原甲藻的生长产生显著影响,最终生物量与初始的氮磷浓度呈正相关,不同培养组的最大生长率存在显著差异(P<0.05);DNA含量与培养液中氮浓度相关性极显著(P<0.01),但与磷浓度相关性不显著(P>0.05);RNA含量与氮磷浓度均呈极显著相关(P<0.01),而且在不同的生长阶段其含量也会发生变化,随着培养时间的延长,各培养组的RNA含量显著下降(P<0.05);RNA/DNA比值与氮磷浓度均显著相关(P<0.05),而且与生长率呈线性相关关系。培养液中氮浓度显著影响到东海原甲藻单位细胞总蛋白含量,磷缺乏也会使总蛋白含量显著降低。  相似文献   

藤壶科DNA 分类研究   总被引：2，自引：1，他引：1  

原帅  安建梅  沙忠利 《海洋科学》2012,36(9):82-88

围胸总目藤壶科的分类系统经历了二亚科系统(小藤壶亚科 Chthamalinae,藤壶亚科 Balaninae)、三亚科系统[藤壶亚科(Balaninae),巨藤壶亚科(Megabalaninae),凹藤壶亚科(Concavinae)],现在采用的是四亚科系统[藤壶亚科(Balaniae)、纹藤壶亚科(Amphibalanus)、巨藤壶亚科(Megabalaninae)和凹藤壶亚科(Concavinae)],但各亚科之间的系统演化关系尚未进行过分子系统学方面的研究.许多藤壶科物种存在趋同进化的趋势,致使传统的形态分类存在困难,不能正确地进行鉴别.本文测定了藤壶科3个亚科里个代表种的线粒体 COI,16S 和12S 基因的部分序列,结合 GenBank 中藤壶科其他物种的12S,28S和18S等基因序列,比较了不同基因片段作为鉴别藤壶科物种的条形码的可行性和有效性,并联合16S和12S序列初步分析了藤壶科各亚科之间的一些亲缘关系.研究结果表明:COI基因的种间和种内遗传距离有明显的间隔区, COI最小种间距离为0.122,远大于最大种内距离0.023,而16S基因的种间与种内距离存在覆盖,最小种间距离为0.018,小于最大种内距离0.023,因此表明,线粒体基因 COI能更准确地鉴定藤壶科种间以及种内关系,并得出阈值为种内差异小于0.023,种间差异大于0.1.ML和BI系统发育分析结果基本一致,支持4亚科的分类系统;巨藤壶亚科形成明显单系群,支持率很高,而两种纹藤壶和管藤壶聚成一支,形成一个单系,本结果支持Newman & Ross的假说,即纹藤壶属和管藤壶属应合并.  相似文献   

不同保存条件下中华哲水蚤基因组DNA提取的比较   总被引：4，自引：2，他引：4       下载免费PDF全文

方旅平  林元烧  曹文清 《海洋科学》2005,29(2):1-4

以采自厦门港的中华哲水蚤(Calanus sinicus)为对象，研究多种保存条件对中华哲水蚤基因组DNA提取的影响。结果表明，除5％福尔马林保存的样品没有提取到DNA之外，其余样品均能成功提取出基因组DNA；以该DNA为模板通过相应引物成功扩增出线粒体DNACOI基因片段。其中无水乙醇保存样品提取可靠的基因组DNA方法的建立，为野外样品保存工作提供了一个简便，经济的途径。  相似文献   

Genotoxicity in two bivalve species from a coastal lagoon in the south of Portugal     

《Marine environmental research》2013

DNA damage was evaluated by comet assay in the haemolymph of two bivalve species Ruditapes decussatus and Mytilus galloprovincialis from the Ria Formosa lagoon (south Coast of Portugal). Clams and mussels were sampled from sites close to each other to determine interspecific responses to similar environmental conditions, considering also seasonal and gender differences. Coupled with genotoxic effect, another damage biomarker (lipid peroxidation) was analysed to verify if the conditions that instigate DNA damage can be related with injury to cell membranes. For both species, DNA damage was low, reflecting the low levels of genotoxic contaminants in the lagoon, and no interspecific differences were found. However, seasonal differences were established for both bivalve species, reflecting higher environmental stress in summer. Regarding gender susceptibility, only clams showed differences in percentage of Tail DNA, with females more sensitive to DNA damage than males. Additionally, results for clams point out that factors responsible for LPO may not be the same as those causing genotoxicity. This study demonstrated that DNA damage is a sensitive biomarker to discriminate spatial, temporal and gender differences, being an appropriate biomarker for genotoxicity evaluation even in places of low contamination, such as the Ria Formosa lagoon.  相似文献   

Non-destructive sampling of juvenile abalone using epipodial tentacles and mucus: method and application     

《African Journal of Marine Science》2013,35(3-4):719-721

Good-quality biological material is needed to obtain intact deoxyribonucleic acid (DNA) for use in molecular techniques such as the polymerase chain reaction (PCR). Non-destructive sampling protocols of juvenile abalone Haliotis midae (7–15 months old) were tested in order to collect material for DNA extraction. DNA was successfully extracted from epipodial tentacles and mucus samples. PCR results confirmed the good quality of the DNA and the reliability of the method.  相似文献