| 东海原甲藻核酸和蛋白对环境中氮磷浓度变化的响应 |
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| 引用本文： | 刘云,宋秀贤,曹西华,袁涌铨,俞志明.东海原甲藻核酸和蛋白对环境中氮磷浓度变化的响应[J].海洋学报,2012,34(2):153-161. |
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| 作者姓名： | 刘云 宋秀贤 曹西华 袁涌铨 俞志明 |
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| 作者单位： | 1.中国科学院 海洋研究所 海洋生态与环境科学重点实验室, 山东 青岛 266071;中国科学院 研究生院, 北京 100049 |
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| 基金项目： | 国家自然科学基金(30970522);国家重点基础研究发展计划("九七三"项目,2010CB428706);国家"八六三"计划专题项目(2008AA09Z107)。 |
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| 摘 要： | 通过设定不同的氮(N)、磷(P)浓度一次性培养东海原甲藻,研究了该藻的生长状况及不同生长阶段单位细胞DNA,RNA及蛋白质含量的变化,结果表明,不同氮磷浓度对东海原甲藻的生长产生显著影响,最终生物量与初始的氮磷浓度呈正相关,不同培养组的最大生长率存在显著差异(P<0.05);DNA含量与培养液中氮浓度相关性极显著(P<0.01),但与磷浓度相关性不显著(P>0.05);RNA含量与氮磷浓度均呈极显著相关(P<0.01),而且在不同的生长阶段其含量也会发生变化,随着培养时间的延长,各培养组的RNA含量显著下降(P<0.05);RNA/DNA比值与氮磷浓度均显著相关(P<0.05),而且与生长率呈线性相关关系。培养液中氮浓度显著影响到东海原甲藻单位细胞总蛋白含量,磷缺乏也会使总蛋白含量显著降低。
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| 关 键 词： | 东海原甲藻 DNA RNA RNA/DNA比值 蛋白质 生长率 |
| 收稿时间： | 6/1/2011 12:00:00 AM |
| 修稿时间： | 2011/9/26 0:00:00 |
Responses of nucleic acid and protein content in Prorocentrum donghaiense Lu to different nitrogen and phosphorus concentrations |
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| LIU Yun,SONG Xiuxian,CAO Xihu,YUAN Yongquan and YU Zhiming.Responses of nucleic acid and protein content in Prorocentrum donghaiense Lu to different nitrogen and phosphorus concentrations[J].Acta Oceanologica Sinica (in Chinese),2012,34(2):153-161. |
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| Authors: | LIU Yun SONG Xiuxian CAO Xihu YUAN Yongquan and YU Zhiming |
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| Institution: | 1.Key Laboratory of Marine Ecology and Environmental Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Graduate School, Chinese Academy of Sciences, Beijing 100049, China2.Key Laboratory of Marine Ecology and Environmental Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China |
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| Abstract: | Changes in growth, cellular DNA, RNA and total protein content related to nitrogen(N) and phosphorus(P) concentrations were studied in a marine dinoflagellate Prorocentrum donghaiense Lugrown in batch cultures. The results show that the growth of P. donghaiense is significantly affected by N and P concentrations. The ultimate biomass depended on the initial N and P concentrations. The maximum growth varied distinctly under different N or P treatments(P<0.05). DNA content had significant correlation with N concentrations in the cultures but not with P. RNA content was strongly associated with both N and P concentrations(P<0.01). Different growth stages could also change cellular RNA content. The RNA content decreased along with the extension of experimental time.RNA/DNA ratio had significant correlation with the N and P concentrations. There appeared to be a linear relationship between the growth rate and the ratio of RNA/DNA. Cellular total protein content was significantly affected by N concentrations. P deficiency could also reduce cellular protein content. |
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| Keywords: | Prorocentrum donghaiense Lu DNA RNA RNA/DNA protein growth rate |
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