长江下游江段铜鱼肌肉营养成分分析   总被引：7，自引：0，他引：7  

刘凯  徐东坡  段金荣  张敏莹  虞锐鹏  施炜纲 《广东海洋大学学报》2008,28(3)

对采自长江下游江段的铜鱼(Coreius heterodom)肌肉营养成分进行了测定和分析。结果表明,铜鱼肌肉水分、粗蛋白质、粗脂肪和粗灰分占鲜样的比例分别为:70.03%、20.54%、6.82%和1.38%;按照氨基酸评分(Amino Acid Score,AAS)和化学评分(Chemical Score,CS)标准,铜鱼限制性氨基酸为缬氨酸和异亮氨酸,必需氨基酸指数(EAAI)为81.52;不饱和脂肪酸和ω-3多不饱和脂肪酸含量分别为63.03%和2.0%;肌肉中常、微量元素含量最高分别为钾(3135μg/g)和铁(5.46μg/g),锌铜比和锌铁比分别为8.85和0.65。  相似文献   

海上高温低渗油田酸化技术应用研究     

彭雪飞  ;冯浦涌  ;黄雷  ;张海  ;司念亭  ;李君宝 《海洋地质译丛》2008,(2):73-76

针对渤中34油田油藏孔隙度渗透率比较低、温度比较高（大于120℃）的特点,结合储层伤害原因分析,根据室内试验结果,采用多氢酸体系对渤中34油田的油井进行酸化增产作业。通过对渤中34油田5口井（均为新井,完井后基本不出液）的酸化施工,酸化成功率达到100％,酸化后各井均达到甚至超过配产要求,这表明多氢酸体系对于高温低渗砂岩地层,能够有效地解除钻完井过程中造成的污染。与常规酸液体系相比,多氢酸体系具有明显的优势且效果明显,对于类似海上油田酸化有较强的借鉴意义。  相似文献   

Effects of nutritional factors on the growth and heterotrophic eicosapentaenoic acid production of diatom <Emphasis Type="Italic">Nitzschia laevis</Emphasis>     

Xiaohong Cao  Songyao Li  Chunling Wang  Meifang Lu 《中国海洋大学学报(英文版)》2008,7(3):333-338

The effects of several nutritional factors on the growth and eicosapentaenoic acid (EPA) production of diatom Nitzschia laevis were studied. 4 LDM (quadrupled concentration of the nutrient salt) was the optimal concentration of nutrient salt for the growth and EPA production of N. laevis. The growth of N. laevis was inhibited when the glucose concentration was either lower than 10 gL⁻¹ or higher than 15 gL⁻¹. Both sodium nitrate and urea were good nitrogen sources for the growth and EPA production, while ammonium chloride seriously decreased the dry cell weight (DW) and the EPA content. Silicate seriously influenced the growth of N. laevis. The maximum DW of 2.34 gL⁻¹ was obtained in the presence of 150 mgL⁻¹ Na₂SiO₃·9H₂O. The EPA content remained almost the same when the silicate concentration was lower than 150 mgL⁻¹; however, higher silicate concentrations resulted in a steady decrease of EPA content. Low medium salinity (⩽29) did not seem to influence the DW of N. laevis, and high salinity resulted in a decrease of DW. The highest EPA content (4.08%) and yield (110 mgL⁻¹) were observed at the salinity of 36 and 29, respectively.  相似文献   

Study on the isotherms of the interaction between suspended particles and Cu (II) in the Huanghe River   总被引：4，自引：0，他引：4  

Li Gai-zhi  Guo Bo-shu  Liu Ying  Hong Xia  Li Jing-feng  Li Bei-gang 《中国海洋湖沼学报》2000,18(2):178-182

The isotherms of the interaction between the suspended particles and Cu²⁺, and the effects of lysine and asparaginic acid on the isotherms in the Huanghe (Yellow) River were studied by applying the theory and method of interfacial stepwise ion/coordination particle exchange. We obtained a new stepped river isotherm, formed by two curves joined together with a “plateau” in the middle. The adsorption equilibrium constantsK ₁ andK ₂ were calculated by using the isothermal equation of surface stepwise ion exchange. Amino acid in small amount promotes exchange adsorption of the suspended particles with Cu²⁺. The degree of promotive action relates to the isoelectric point of amino acid. The promotive effect of lysine is bigger than that of asparaginic acid. Project 29361001 supported by NSFC.  相似文献   

Total lipid and fatty acid composition of eight strains of marine diatoms   总被引：3，自引：0，他引：3  

Liang Ying  Mai Kang-sen  Sun Shi-chun 《中国海洋湖沼学报》2000,18(4):345-349

Fatty acid composition and total lipid content of 8 strains of marine diatoms (Nitzschia frustrula, Nitzschia closterium, Nitzschia incerta, Navicula pelliculosa, Phaeodactylum tricornutum, Synedra fragilaroides) were examined. The microalgae were grown under defined conditions and harvested at the late exponential phase. The major fatty acids in most strains were 14∶0 (1.0%–6.3%), 16∶0 (13.5–26.4%), 16∶1n−7 (21.1%–46.3%) and 20∶5n−3 (6.5%–19.5%). The polyunsaturated fatty acids 16∶2n−4, 16∶3n−4, 16∶4n−1 and 20∶4n−6 also comprised a significant proportion of the total fatty acids in some strains. The characteristic fatty acid composition of diatoms is readily distinguishable from those of other microalgal groups. Significant concentration of the polyunsaturated fatty acid 20∶5n−3 (eicosapentaenoic acid) was present in each strain, with the highest proportion in B222 (19.5%). Project supported by the Hi-Tech “863” Programs of the China Ministry of Science and Technology (863-819-02-01).  相似文献   

马氏珠母贝TRACP基因的克隆及组织表达     

郑哲  吴宣瑾  焦钰  黄荣莲  杜晓东 《广东海洋大学学报》2019,(3):24-29

【目的】克隆马氏珠母贝(Pinctada martensii)抗酒石酸酸性磷酸酶(Tartrate resistant acid phosphatase,TRACP)基因,分析该基因在不同组织中的表达模式。【方法】用RACE技术克隆得马氏珠母贝TRACP基因(PmTRACP),用实时荧光定量PCR分析该基因在外套膜、闭壳肌、足、性腺、珍珠囊、肝胰腺和鳃中的表达。【结果与结论】PmTRACP基因长度为2 034 bp,开放式阅读框972 bp,编码323个氨基酸,5′UTR长度为27 bp,3′UTR长度为1 035 bp。预测PmTRACP分子质量约为36.39 ku,理论等电点为5.97。该基因含有一个钙调神经磷酸酶样磷酸酯酶结构域。PmTRACP与其他物种TRACP的同源性为48%～65%,与长牡蛎(Crassostrea gigas)TRACP的同源性最高,同时D³²、D⁷⁰、Y⁷³、N¹⁰⁸、H²⁰³、H²¹²、H²³⁷、H²³⁹等8个氨基酸活性位点和N¹¹⁴糖基化位点在不同物种TRACP中高度保守。PmTRACP与长牡蛎TRACP的亲缘关系最近。PmTRACP在马氏珠母贝各个组织均有表达,且在肝胰腺和珍珠囊中高表达。  相似文献   

Response of Microbial Communities in Soil to Multi-level Warming in a Highland Barley System of the Lhasa River     

FU Gang  SUN Wei  LI Shaowei  ZHONG Zhiming 《资源与生态学报(英文版)》2019,10(4):373-378

No studies have examined the effect of experimental warming on the microbial biomass and community composition of soil in agricultural ecosystem on the Qinghai-Tibet Plateau. Thus it is unclear whether the influences of experimental warming on microbial communities in soil are related to warming magnitude in croplands on this Plateau. This study performed warming experiment (control, low- and high-level) in a highland barley system of the Lhasa River in May 2014 to examine the correlation between the response of microbial communities in soil to warming and warming magnitude. Topsoil samples (0-10 and 10-20 cm) were collected on September 14, 2014. Experimental warming at both low and high levels significantly increased soil temperature by 1.02 ℃ and 1.59 ℃, respectively at the depth of 15 cm. Phospho lipid fatty acid (PLFA) method was used to determine the microbial community in soil. The low-level experimental warming did not significantly affect the soil’s total PLFA, fungi, bacteria, arbuscular mycorrhizal fungi (AMF), actinomycetes, gram-positive bacteria (G⁺), gram-negative bacteria (G^-), protozoa, the ratio of fungi to bacteria (F/B ratio), and ratio of G⁺ to G^- (G⁺/G^- ratio) at the 0-10 and 10-20 cm depth. The low-level experimental warming also did not significantly alter the composition of microbial community in soil at the 0-10 and 10-20 cm depth. The high-level experimental warming significantly increased total PLFA by 74.4%, fungi by 78.0%, bacteria by 74.0%, AMF by 66.9%, actinomycetes by 81.4%, G⁺ by 67.0% and G^- by 74.4% at the 0-10 cm depth rather than at 10-20 cm depth. The high-level experimental warming significantly altered microbial community composition in soil at the 0-10 cm depth rather than at 10-20 cm depth. Our findings suggest that the response of microbial communities in soil to warming varied with warming magnitudes in the highland barley system of the Lhasa River.  相似文献   

不同氮浓度条件下红色赤潮藻脂肪酸含量及组成特征     

刘云  陈田田  宋书群  李才文 《海洋与湖沼》2019,50(2):301-307

在不同氮浓度条件下对红色赤潮藻(Akashiwosanguinea)进行了一次性培养,研究了其生长过程中脂肪酸含量及组成变化。结果表明:红色赤潮藻脂肪酸含量及组成受初始氮浓度及生长阶段的影响。低氮处理组(0—25μmol/L)脂肪酸含量[(150.02±54.84)—(166.83±46.46)mg/gdw]显著高于其他处理组(P0.05),883μmol/L组最低,约为(86.70±5.66)mg/gdw;各培养组脂肪酸含量随着培养时间的延长而增加,稳定期时脂肪酸含量最高,指数生长前期含量最低。红色赤潮藻含28种脂肪酸,以C16:0、C18:1、C20:5ω3(EPA)和C22:6ω3(DHA)为主,且DHA相对含量较高,最高可占总脂肪酸含量的30.46%。随着初始氮浓度的增加,红色赤潮藻饱和脂肪酸(SFA)和单不饱和脂肪酸(MUFA)在总脂肪酸中所占比例下降,而多不饱和脂肪酸(PUFA)所占比例升高。在红色赤潮藻生长过程中,MUFA相对含量在稳定期时较高,而PUFA相对含量在指数生长前期较高。研究结果可为进一步揭示红色赤潮藻赤潮应对环境胁迫的生理响应机制提供依据。  相似文献   

南海沉积物细菌胞外蛋白酶在家族水平上的多样性   总被引：2，自引：0，他引：2  

杨金玉  冯阳阳  陈秀兰  解彬彬  张玉忠  石梅  张熙颖 《海洋学报(英文版)》2019,38(12):73-83

海洋产蛋白酶细菌及其分泌的胞外蛋白酶在降解有机氮以推动海洋氮循环进行方面发挥着重要作用,但目前对这二者多样性的认识非常有限。本研究自南海沉积物中筛选得到90株产蛋白酶细菌,并通过N-端氨基酸序列,分析了其胞外蛋白酶在家族水平上的多样性。16S rRNA基因序列分析的结果表明,筛选的产蛋白酶细菌均属于Gammaproteobacteria纲,且大多数属于Alteromonadales目和Vibrionales目的不同属。对其中14株菌株的14个胞外蛋白酶的N-端氨基酸序列分析表明,所有这些蛋白酶属于金属蛋白酶的M4家族或丝氨酸蛋白酶的S8家族。本研究提供了海洋沉积物产蛋白酶细菌在类群及其胞外蛋白酶在类型上的新细节,这将有助于全面了解微生物酶促降解海洋沉积物有机氮的过程和机理。  相似文献   

连作马铃薯根系分泌物鉴定及其对尖孢镰孢菌(Fusarium oxysporum)的作用     

谢奎忠  邱慧珍  胡新元  罗爱花 《中国沙漠》2021,41(3):7-15

为了揭示马铃薯连作化感物质与枯萎病之间的关系,通过水培和田间长期定位试验相结合方法,收集不同连作年限马铃薯根系分泌物,采用GC-TOF-MS进行分离鉴定.结果表明:在检测到的马铃薯根系分泌的48种物质中有机酸占30种.比较轮作、连作5年和连作10年马铃薯根系分泌物中有机酸的相对含量,发现苹果酸和棕榈酸的差异比较大.马铃...  相似文献