Closely related thyasirid bivalves associate with multiple symbiont phylotypes          下载免费PDF全文

Rebecca T. Batstone  Suzanne C. Dufour 《Marine Ecology》2016,37(5):988-997

Species of thyasirid bivalves are considered to be representative of early stages of chemosymbiosis, given that bacterial symbionts occur outside gill epithelial cells, vary among species in their abundance and nutritional importance, and are environmentally acquired. For these reasons, assessing the extent of host–symbiont specificity in thyasirids could provide valuable information on the evolution of chemosymbiosis. We show that individuals of two closely related and sympatric Thyasira cf. gouldi operational taxonomic units collected from three sites in a fjord in Newfoundland, Canada, associate with one of three distinct, closely related symbiont phylotypes. While associations show some site‐specificity, there is flexibility in host–symbiont pairings within the fjord, further supporting an early and relatively unspecific stage of chemosymbiosis in this family. Morphologic differences observed both within and among symbiont phylotypes suggest physiologic variation, possibly induced by small‐scale differences in sedimentary conditions.  相似文献   

A seasonal comparison of prokaryote numbers,biomass and heterotrophic productivity in waters of the KwaZulu-Natal Bight,South Africa     

D Muir  T Kunnen 《African Journal of Marine Science》2016,38(1):S123-S138

The KwaZulu-Natal Bight is a shallow indentation of the eastern seaboard of South Africa, characterised by a narrow (45 km wide) extension of the continental shelf, with a shelf break at about 100 m. It has a complex hydrography: the waters of the bight are derived from the fast-flowing, southward-trending Agulhas Current, which is fed mostly by the tropical and subtropical surface waters of the South-West Indian Ocean subgyre, which are generally oligotrophic in nature, notably depleted in reduced nitrogen and phosphate except at river mouths and during periodic upwelling of deeper nutrient-rich water. Despite this, the bight is believed to be relatively productive, and it is suggested that efficient nutrient recycling by prokaryotes may sustain primary productivity efficiently, even in the absence of new nutrient inputs. Here we have measured bacterial numbers, biomass and heterotrophic productivity during summer and winter in conjunction with phytoplankton standing stock and factors that influence it. Bacterial distribution closely matched phytoplankton distribution in surface waters, and was highest close to the coast. Bacterial standing stocks were similar to those of oligotrophic systems elsewhere (0.5–5.0 × 10⁵ cells ml^–1; 1 × 10^–8 to 1.25 × 10^–7 g C ml^–1) and increased in association with the development of phytoplankton blooms offshore and with inputs of allochthonous material by rivers at the coast. Heterotrophic productivity in summer was lowest in the far south and north of the bight (0.5 × 10^–10 g C ml^–1 h^–1) but higher close to the shore, over shallow banks, and in association with increased phytoplankton abundance over the midshelf (1.0–3.5 × 10^–9 g C ml^–1 h^–1). There were marked seasonal differences with lower bacterial standing stocks (5 × 10⁴ to 2 × 10⁵ cells ml^–1; 4–5 × 10^–9 to 1–2 × 10^–8 g C ml^–1) and very low bacterial productivity (4 × 10^–11 to 1 × 10^–10 g C ml^–1 h^–1) in winter, probably resulting from lowered rates of primary productivity and dissolved organic matter release as well as reduced riverine allochthonous inputs during the winter drought.  相似文献   

元素分析仪-同位素比值质谱测量碳氮同位素比值最佳反应温度和进样量的确定   总被引：2，自引：2，他引：0  

徐丽  邢蓝田  王鑫  李中平  毛俊丽 《岩矿测试》2018,37(1):15-20

沉积有机质的碳氮稳定同位素值是进行古气候、古环境及生态系统研究不可或缺的主要研究手段,目前碳氮同位素主要利用元素分析仪-同位素比值质谱(EA-IRMS)系统来测定。EA-IRMS测定过程中的反应温度及样品进样量直接影响反应物在测试中的燃烧程度,从而影响测试数据的精度。本文利用EA-IRMS技术,以标准样品为参考,在不同转化温度下测试碳氮同位素值,研究保证测试精度的最佳反应温度条件;同时,通过分析不同含氮量样品的检测限,明确了样品含氮量与最低检测限之间的关系,确定了精确测定氮同位素值的最低进样量。结果表明:反应温度对测试精度有显著影响,在碳同位素测定时,将反应温度设定为900℃或以上时测试精度均能达到±0.2‰;氮同位素测定时,反应温度须设定为950℃时测试精度才能达到±0.3‰。实验得出样品含氮量与检测限之间的线性相关性为R2=0.873,开展氮同位素测定时可根据此关系来判断和控制进样量。  相似文献   

季节性低温胁迫下陇东黄土高原油污土壤环境因子对耐冷混合菌场地生态修复的响应     

井明博  王金成  段春燕  杨蕊琪  任鹏  吴胜伟 《冰川冻土》2018,40(6):1242-1254

为了揭示季节性低温胁迫下陇东黄土高原油污土壤环境因子对耐冷混合菌场地生态修复的响应机制,利用自主筛选构建的耐冷石油降解混合菌在甘肃省庆阳市庆城县马岭镇长庆油田陇东油泥处理站开展了为期7个月的场地修复实验,采用常规方法测定了不同季节土壤理化特性、酶活性和微生物群落特性等环境指标。结果表明：（1）在季节性低温胁迫下（9-11月）M2组（耐冷混合菌处理组）月平均降解率明显增加（P<0.01）,JZJ+M1组（金盏菊联合常温混合菌处理组）和M2组累计TPH降解率分别为15.37%±3.51%和28.64%±4.12%。（2）M2组土壤脱氢酶和多酚氧化酶在低温季节（LT）活性最高,且温度和处理存在显著交互作用（P<0.01）。在土壤营养元素方面,无论何种处理方式（JZJ+M1和M2）二者含量均为RT（常温季节）高于LT（P<0.05）,同时明显高于CK组（P<0.05）。（3）M2组土壤微生物群落Shannon-Wiener指数和均匀度指数在LT高于RT（P<0.05）。（4）NMDS和Pearson相关性分析结果显示,M2组在低温季节具有较高TPH降解率主要与土壤多酚氧化酶、脱氢酶、单月TPH降解率（μ2）和Shannon-Wiener指数有关,且均呈极显著正相关关系。通过分析环境因子的季节响应,优化场地修复在低温环境的降解条件、加速低温期石油烃降解速率,以期为陇东地区低温耐冷混合菌场地生态修复技术的推广应用提供参考和基础数据资料。  相似文献   

元古宙真核藻类演化及环境控制因素     

张凤廉  王华建  张水昌  邓胜徽  叶云涛  邓焱  王晓梅  吕丹  卢远征  吕怡潼 《地质学报》2021,95(5):1334-1355

宜居地球、地外生命探索以及资源勘查等的需求使真核生物的早期溯源和演化趋势研究成为国际热点.根据已发现实体化石、分子化石和分子钟证据,将元古宙真核藻类演化划分为环境准备(2.45～1.70 Ga)、缓慢发展(1.70～0.80 Ga)、剧烈波动(0.80～0.64 Ga)、快速辐射(0.64～0.54 Ga)四个阶段.元古宙真核生物的出现、演化和辐射进程与地球氧化和极端气候事件(如冰期)的发生具耦合性,表现出早期生命与地球表层环境的协同演化.真核藻类在1.70～0.80 Ga期间的缓慢演化可能与长期较低的大气氧含量(约为现今水平的1％～10％PAL)有关.低的大气-海洋氧化程度不仅限制了真核藻类生存空间,也通过对氮、磷等营养元素的供应约束,限制了真核藻类初级生产力水平.因此,地球表层氧化可能是地球宜居演化,并孕育出真核生物等各种复杂生命的主要原因.从地球系统形成与演变的角度探索生物圈演化或能对生命的过去和未来给出更为可靠的答案.  相似文献   

二价铁氧化对铁锰循环功能微生物活性的影响及机制          下载免费PDF全文

屈婧祎  童曼  袁松虎 《地球科学》2021,46(2):632-641

地质微生物是沉积环境中铁、锰氧化还原循环的主要驱动因子,铁锰共存环境中二价铁氧化对不同铁、锰循环功能微生物活性的影响差异和机制尚不清楚.以铁还原菌Shewanella oneidensis MR-1、铁氧化菌Pseudogulbenkiania sp.strain 2002、锰氧化菌Pseudomonas putida MnB1和Leptothrix discophora SS-1作为代表性的铁、锰循环功能微生物,利用平板计数、荧光显微镜等手段探究了Fe(II)氧化对功能微生物活性的影响差异及机制.结果表明0.05 mM Fe2+氧化60 min可使MR-1和MnB1的活菌数量降低4~5个数量级,SS-1及S.2002无显著失活.Fe(II)氧化产生的吸附态。OH和胞内。OH是细菌失活的主要原因,胞外H₂O₂、胞外游离态。OH和三价铁氧化物是细菌失活的次要原因,SS-1及S.2002产生了氧化应激反应,成功抵御了活性氧化物种.   相似文献   

硫介导细菌还原载砷铁矿对砷迁移转化的影响          下载免费PDF全文

王晶  谢作明  王佳  杨洋  刘恩杨 《地球科学》2021,46(2):642-651

硫在铁和砷的生物地球化学循环中发挥着重要作用,但地下水系统中硫循环的中间产物S(0)对细菌转化铁和砷的影响尚不清楚.采用室内模拟实验,研究硫参与下细菌D2201对液相和载砷针铁矿中Fe(III)和As(V)的还原作用.结果表明:细菌D2201具有很强的铁还原能力,可以将液相中74%的Fe(III)还原;加入硫后,细菌还原S(0)产生的S(-II)使铁还原率提高到94%.但是,硫没有明显影响细菌对砷的还原.在实验初期,细菌明显加速了载砷针铁矿的还原,最终还原释放到液相中的Fe(II)浓度为32.12 μmol/L;硫的加入增强了细菌对载砷针铁矿的还原,还原溶解的Fe(II)增加至284.13 μmol/L,同时,砷的释放量也增加了1.6倍.这些结果表明硫显著促进了细菌对针铁矿的还原溶解并加速砷的释放.XRD和SEM-EDS结果显示,细菌还原针铁矿但不改变其矿相,而硫的加入也仅使矿物发生一定程度的团聚,并没有使其转变为其他矿物,也未导致砷的再吸附.   相似文献   

土地利用方式对岩溶断陷盆地土壤细菌和真核生物群落结构的影响     

李强 《地球学报》2021,42(3):417-425

土壤生物是土壤生态系统的重要组成部分,在生态系统物质循环和能量转化中占有重要的地位.为揭示土地利用方式对岩溶断陷盆地土壤生物的影响,在获取土壤理化性质及碳氮磷化学计量学特征的基础上,利用16S rRNA和18S rRNA高通量测序技术研究了弃耕地、草地、人工林和天然林等土壤细菌与真核生物的群落结构.C:N、C:P和N:P比变化规律为弃耕地<草地<人工林<天然林,土壤细菌和真核生物α多样性、互作网络表现出相反的变化规律.土壤磷、钾和钙是影响岩溶断陷盆地土壤细菌和真核生物群落结构和多样性的关键因素.RB41是决定岩溶断陷盆地土壤细菌-真核生物网络系统的关键类群.本研究结果为利用土壤生物进行石漠化治理及岩溶生态修复提供了理论基础.  相似文献   

Confronting the nitrogen challenge: Options for governance and target setting     

《Global Environmental Change》2019

The release of excessive anthropogenic nitrogen contributes to global climate change, biodiversity loss, and the degradation of ecosystem services. Despite being an urgent global problem, the excess nitrogen is not governed globally. This paper considers possible governance options for dealing with excessive nitrogen through target setting, which is an approach commonly adopted to address global environmental problems. The articulation of the nitrogen problem and the numerous international institutions dealing with it, provide evidence of a nitrogen regime characterised by limited coordination and targets covering sources and impacts only partially. This calls for improving the nitrogen governance in the direction of more integrated approaches at the global scale. In this vein, the paper investigates two opposite governance options – here labelled as ‘holistic’ and ‘origin-based’ – and evaluates them for their capability to define solutions and targets for human-induced nitrogen. From the analysis, it emerges that origin-based solutions can be preferable to holistic solutions as they can be more specific and potentially have greater immediate results. Independent from which governance arrangement is chosen, what matters most is the speed at which an arrangement can deploy solutions to combat (fast-growing) nitrogen pollution.  相似文献   

A dual-porosity model for nitrogen leaching from a watershed/Un modèle à double porosité pour le lessivage de l’azote d’un bassin versant     

《水文科学杂志》2013,58(2)

Abstract

Abstract The MASONW (MACRO + SOILN + Watershed) model describing nitrogen leaching in watersheds was developed and tested. The model is based on the MACRO and SOILN models. The dual-porosity model MACRO simulates water flow on the field scale. The SOILN model describes turnover and leaching of nitrogen. Two main features of a watershed have been added into these two models: (a) the existence of a river system, and (b) variable thickness of the aeration zone within a watershed. Good agreement between the output of the MASONW model and observed data for water discharge and nitrate concentrations were achieved in the Odense watershed (496 km²) in Denmark.  相似文献