麻痹性贝毒毒素研究Ⅰ.塔玛亚历山大藻的麻痹性贝毒毒素对运动神经末梢和全细胞钠离子通道的抑制作用研究     

王云峰  于仁诚  李钧  颜天  周名江 《海洋科学集刊》2003,(45):132-137

麻痹性贝毒(Paralytic Shellfish Poisoning，PSP)毒素由石房蛤毒素(saxitoxin，STX)及其衍生物组成，目前己发现20余种，在赤潮研究、分子生物学和神经生物学基础研究、医药、军事防化等方面都有应用潜力[其结构、类型和应用见本集刊王云峰等(2003)“麻痹性贝毒毒素的应用研究进展”一文]。由于PSP毒素的稀有来源和国际社会对STX交易的禁止，限制了国内PSP毒素应用及研究的全面深入展开，因此本文作者对PSP毒素进行了制备，并采用不同方法对制备的PSP毒素进行了研究。 PSP毒素能够选择性地可逆抑制可兴奋膜的电压依赖钠离子通道的开放，从而阻止神经冲动的发生和传导，使神经、肌肉丧失兴奋性(Frace et al.，1986；Penzotti et al.，1998)。本文利用神经束膜下记录和全细胞膜片钳技术，报道了从塔玛亚历山大藻(Alexandrium tamarense)中提取的PSP粗毒素对小鼠运动神经末梢膜电流和NG108-15细胞钠离子通道的作用研究结果，并与STX标准毒素的作用结果进行了比较。 NG108-15细胞是由小鼠神经母细胞瘤和大鼠胶质细胞瘤融合的杂交细胞，经分化剂分化后，显示诸+L1196如兴奋性、合成和释放乙酰胆碱、与培养肌细胞形成突触联系等多种神经细胞的基本特性(Hamprcht，1977)和Na+、K+、Ca2+等多种离子通道，已作为神经细胞模型被广泛应用于分析药物对离子通道作用的研究(Enomoto et al.，1992； Docherty et al.，1992；Shi et al.，1993；Hu et al.，1997a；Hu et al.，1997b)；发育出具有Na+内流支持的锋电位(Hamprecht,1977)，该电位是分析作用于膜钠离子通道药物的好材料。  相似文献   

Barotropic Response of the Sea of Okhotsk to Wind Forcing     

Daisuke Simizu  Kay I. Ohshima 《Journal of Oceanography》2002,58(6):851-860

We have examined wind-induced circulation in the Sea of Okhotsk using a barotropic model that contains realistic topography with a resolution of 9.25 km. The monthly wind stress field calculated from daily European Centre for Medium-Range Weather Forecasting (ECMWF) Re-Analysis data is used as the forcing, and the integration is carried out for 20 days until the circulation attains an almost steady state. In the case of November (a representative for the winter season from October to March), southward currents of velocity 0.1–0.3 m s⁻¹ occur along the bottom contours off the east of Sakhalin Island. The currents are mostly confined to the shelf (shallower than 200 m) and extend as far south as the Hokkaido coast. In the July case (a representative for the summer season from April to September), significant currents do not occur, even in the shallow shelves. The simulated southward current over the east Sakhalin shelf appears to correspond to the near-shore branch of the East Sakhalin Current (ESC), which was observed with the surface drifters. These seasonal variations simulated in our experiments are consistent with the observations of the ESC. Dynamically, the simulated ESC is interpreted as the arrested topographic wave (ATW), which is the coastally trapped flow driven by steady alongshore wind stress. The volume transport of the simulated ESC over the shelf reaches about 1.0 Sv (1 Sv = 10⁶ m³s⁻¹) in the winter season, which is determined by the integrated onshore Ekman transport in the direction from which shelf waves propagate. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Atmospheric forcing of the eastern tropical Pacific: A review   总被引：1，自引：8，他引：1  

Jorge A. Amador  Eric J. Alfaro  Omar G. Lizano  Victor O. Magaa 《Progress in Oceanography》2006,69(2-4):101

The increase in marine, land surface, atmospheric and satellite data during recent decades has led to an improved understanding of the air–sea interaction processes in the eastern tropical Pacific. This is also thanks to extensive diagnoses from conceptual and coupled ocean–atmosphere numerical models. In this paper, mean fields of atmospheric variables, such as incoming solar radiation, sea level pressure, winds, wind stress curl, precipitation, evaporation, and surface energy fluxes, are derived from global atmospheric data sets in order to examine the dominant features of the low level atmospheric circulations of the region. The seasonal march of the atmospheric circulations is presented to depict the role of radiative forcing on atmospheric perturbations, especially those dominating the atmosphere at low levels.In the tropics, the trade winds constitute an important north–south energy and moisture exchange mechanism (as part of the low level branch of the Hadley circulation), that determines to a large extent the precipitation distribution in the region, i.e., that associated with the Inter-Tropical Convergence Zone (ITCZ). Monsoonal circulations also play an important role in determining the warm season precipitation distribution over the eastern tropical Pacific through a large variety of air–sea–land interaction mechanisms. Westward traveling waves, tropical cyclones, low latitude cold air intrusions, and other synoptic and mesoscale perturbations associated with the ITCZ are also important elements that modulate the annual rainfall cycle. The low-level jets of the Gulf of California, the Intra-Americas Sea (Gulf of Mexico and Caribbean Sea) and Chocó, Colombia are prominent features of the eastern tropical Pacific low-level circulations related to sub-regional and regional scale precipitation patterns. Observations show that the Intra-Americas Low-Level Jet intensity varies with El Niño/Southern Oscillation (ENSO) phases, however its origin and role in the westward propagation and development of disturbances that may hit the eastern tropical Pacific, such as easterly waves and tropical cyclones, are still unclear. Changes in the intensity of the trade winds in the Caribbean Sea and the Gulf of Mexico (associated with eastern tropical Pacific wind jets) exert an important control on precipitation by means of wind–topography interactions. Gaps in the mountains of southern Mexico and Central America allow strong wind jets to pass over the continent imprinting a unique signal in sea surface temperatures and ocean dynamics of the eastern tropical Pacific.The warm pools of the Americas constitute an important source of moisture for the North American Monsoon System. The northeastern tropical Pacific is a region of intense cyclogenetic activity, just west of the coast of Mesoamerica. Over the oceanic regions, large-scale properties of key variables such as precipitation, moisture, surface energy fluxes and wind stress curl are still uncertain, which inhibits a more comprehensive view of the region and stresses the importance of regional field experiments. Progress has been substantial in the understanding of the ocean and atmospheric dynamics of the eastern tropical Pacific, however, recent observational evidence such as that of a shallow meridional circulation cell in that region, in contrast to the classic concept of the Hadley-type deep meridional circulation, suggests that more in situ observations to validate theories are still necessary.This paper is part of a comprehensive review of the oceanography of the eastern tropical Pacific Ocean.  相似文献   

现场海域水平扩散系数的探讨　Ⅱ．黄河三角洲埕北海域的计算     

匡国瑞  陈伯海  徐天真  钱成春  俞光耀 《中国海洋大学学报(自然科学版)》1995,(3)

根据Ｔａｙｌｏｒ的湍流扩散理论和Ｈａｙ、Ｐａｓｑｕｉｌｌ提出的拉格朗日流和欧拉流的相关函数的相似性，用黄河三角洲埕北海域的实测资料，按四种不同方案计算出该海域的水平扩散系数和谱密度分布。指出：该海区潮流扩散系数主要受潮流的影响，其分布特征与潮流的分布基本一致，所求的水平扩散系数主要是由周期性的潮运动给予潮流扰动所致。方案４是通过富氏变换滤波消去了周期性潮流扰动，本方案计算的水平扩散系数是由周期为６ｈ以下的小尺度涡提供能量。  相似文献   

An inexpensive instrument for measuring benthic current velocity and direction at sea     

J. R. Craig  S. J. Kennelly 《Estuarine, Coastal and Shelf Science》1991,32(6)

An instrument which measures the velocity and direction of benthic water currents at depths of up to 80 m is described. Characteristics of this meter include: (i) readings are not affected by water flow during sinking or retrieval; (ii) it is portable; (iii) it operates unattended; (iv) it is inexpensive; and (v) it requires only simple maintenance. A timing circuit which operates two time periods in succession is used to activate and de-activate a revolution counter and a fluidfilled compass which are mounted on a special frame and vane system. In the field, the instrument accurately measured average current velocities of up to 6 m s⁻¹. Data are presented which illustrate the usefulness of this instrument in describing the velocity and direction of benthic currents along the east coast of Australia and demonstrate a positive correlation between benthic current velocity and catch rates of the commercially-exploited spanner crab Ranina ranina.  相似文献   

连云港近岸海域物理自净能力研究及水质预测——Ⅲ.西大堤建成后对物质输运的影响     

赵可胜  娄安刚  王学昌 《中国海洋大学学报(自然科学版)》1994,(Z1)

在连云港近岸海域计算潮流场基础上建立拉格朗日余流模型,并对连云港市两大堤建成前后的拉格朗日余流变化进行了分析,且选择有代表性的排污口进行了数值跟踪。  相似文献   

A simple model to calculate the residual flows in a Spanish ria. Hydrographic consequences in the ria of Vigo     

Ricardo Prego  Fernando Fraga 《Estuarine, Coastal and Shelf Science》1992,34(6)

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水槽造流系统水力计算与泵机选择   总被引：1，自引：0，他引：1  

孙东昌  张亭健  辛海英 《海洋科学进展》2002,20(2):125-132

提供一种完整的用于试验水槽造流系统的水力计算和泵机选择方法 ,可用于其他类似工程设计。  相似文献   

4D seismic time-lapse monitoring of an active cold vent,northern Cascadia margin   总被引：1，自引：1，他引：1  

Michael Riedel 《Marine Geophysical Researches》2007,28(4):355-371

Two single-channel seismic (SCS) data sets collected in 2000 and 2005 were used for a four-dimensional (4D) time-lapse analysis of an active cold vent (Bullseye Vent). The data set acquired in 2000 serves as a reference in the applied processing sequence. The 4D processing sequence utilizes time- and phase-matching, gain adjustments and shaping filters to transform the 2005 data set so that it is most comparable to the conditions under which the 2000 data were acquired. The cold vent is characterized by seismic blanking, which is a result of the presence of gas hydrate in the subsurface either within coarser-grained turbidite sands or in fractures, as well as free gas trapped in these fracture systems. The area of blanking was defined using the seismic attributes instantaneous amplitude and similarity. Several areas were identified where blanking was reduced in 2005 relative to 2000. But most of the centre of Bullseye Vent and the area around it were seen to be characterized by intensified blanking in 2005. Tracing these areas of intensified blanking through the three-dimensional (3D) seismic volume defined several apparent new flow pathways that were not seen in the 2000 data, which are interpreted as newly generated fractures/faults for upward fluid migration. Intensified blanking is interpreted as a result of new formation of gas hydrate in the subsurface along new fracture pathways. Areas with reduced blanking may be zones where formerly plugged fractures that had trapped some free gas may have been opened and free gas was liberated.  相似文献   

诊断台风异常路径的三层权重控制点方案     

谢玲娟  林丽珊 《海洋预报》1992,9(3):8-16

本方案采用850、700、500hPa三个层次的环境平均流场作控制点法。综合考虑这些层次上的引导气流在不同时、空域中的相对重要性。试验结果表明,其效果比单一取某层作引导为佳。且对用来预测台风的未来24小时路径是否异常具有一定的参考价值。  相似文献