合浦珠母贝26S蛋白酶体S4,S7亚基基因片段的分离与分析     

姜铁民  张勇  黄洁  谢莉萍  吴移谋  张荣庆 《海洋科学》2003,27(4):28-34

26S蛋白酶体是真核生物中一种具有ATP依赖性的蛋白酶复合体,主要通过泛肽途径选择性降解细胞内与代谢调控、细胞周期有关的功能蛋白及异常蛋白,参与多种细胞活动的调控过程。26S蛋白酶体由具有催化活性的20S亚复合体和一个具有调节作用的19S亚复合体组成,其中19S亚复合体中的ATP酶亚基是调节26S蛋白酶体活性的重要组件。本通过简并引物PCR手段,从软体动物合浦珠母贝(Pinctada fucata)中扩增到参与构成19S亚复合体的S4和S7(MSS1)两个亚基的基因片段。这两个基因片段所编码的ATP酶组件包含有Gx4GKT,DEID,SAT和H／QRxGRxxR等26S蛋白酶体ATP酶亚基的共同功能基序。这是首次在软体动物中报道26S蛋白酶体的ATP酶亚基基因序列,为研究软体动物中26S蛋白酶体的结构与功能奠定了分子基础。  相似文献   

Modal Wave Number Tomography for South China Sea Front   总被引：10，自引：0，他引：10  

彭临慧  王宁  邱晓芳  田纪伟 《中国海洋工程》2003,17(2)

1 .IntroductionTheSouthChinaSealiestothesoutheastpartoftheChinesecontinent.TheKuroshioflowsbytheeastsideoftheLuzonStraitfromsouthtonorthinwinter ,spring ,andautumn .TheKuroshioaf fectsnearcurrentfieldsandtemperaturefields,changingtheoceanenvironmentparametersandsoundpropagation .Thus ,theoceanacoustictomographytechniqueisofpotentialtolong term ,largescalemonitoringoftheocean .Themodalwavenumbertomographymethod (Rajanetal.,1 987;Frisketal.,1 989)isusedtoobtainthesoundspeedprofileinwatercol…  相似文献   

A method to estimate the forces exerted by internal solitons on cylindrical piles   总被引：1，自引：0，他引：1  

Shuqun Cai  Xiaomin Long  Zijun Gan 《Ocean Engineering》2003,30(5):673-689

Internal soliton is the large amplitude wave existing in the pycnocline, induced by internal tide in the condition of special bottom topography. During its propagation process, the induced disturbance can bring about strong convergence of sea water and sudden strong current (wave-induced-current), which can cause severe threat to the ocean engineering structures, such as oil drilling platform and pipeline. In this paper, Morison’s empirical method, modal separation and regression analyses are introduced to estimate the forces and torques exerted by internal soliton on cylindrical piles. As an example, a limited set of observational data recording a passage of the internal soliton near Dongsha Islands is used to estimate the horizontal velocity and its acceleration in a vertical section for computing the force and torque on a supposed pile, and the estimation results are reasonable. It is shown that, the higher number of the modes retained in the calculation, the better the estimation of velocity profile fits the observational one. A better overall approximation to the real solution can be reached if there are more observational current data acquired in a whole vertical profile.  相似文献   

A note on estimation of the Jacobian elliptic parameter in cnoidal wave theory     

Y. -S. Cho   《Ocean Engineering》2003,30(15):1915-1922

A new and simple calculating technique for the Jacobian elliptic parameter is presented in this study. The technique is very useful in generating a train of cnoidal waves in both laboratory and numerical wave tanks. Upon specification of water depth, the wave height and either the wave period or the wavelength, the proposed technique uses the Newton–Raphson method to estimate the Jacobian elliptic parameter directly, without trial and error procedures or look-up in tables. It is shown that the technique provides equally accurate results as the ad hoc methods previously used.  相似文献   

Dynamic properties of green water event in the overtopping of extreme waves on a fixed dock     

Nobuhito Mori  Daniel T. Cox 《Ocean Engineering》2003,30(16):2021-2052

A statistical model is developed to predict wave overtopping volume and rate of extreme waves on a fixed deck. The probability density function for the volume and rate of overtopping water are formulated based on the truncated Weibull distribution with the assumption of local sinusoidal profile for small amplitude waves. Sensitivity to the wave nonlinearity parameter and deck clearance is discussed. The statistical model is compared to laboratory data of the instantaneous free surface elevation measured in front of a fixed deck, and overtopping volume and overtopping rate measured at the leading edge of the deck. The statistical theory compared well with the measured exceedance probability seaward of the deck. The model prediction of the exceedance probability of deck overtopping gave qualitatively good agreement for large overtopping values.  相似文献   

Modelling of periodic wave transformation in the inner surf zone     

P. Bonneton   《Ocean Engineering》2007,34(10):1459-1471

In this paper, we analyse the ability of the nonlinear shallow-water (NSW) equations to predict wave distortion and energy dissipation of periodic broken waves in the inner surf zone. This analysis is based on the weak-solution theory for conservative equations. We derive a new one-way model, which applies to the transformation of non-reflective periodic broken waves on gently sloping beaches. This model can be useful to develop breaking-wave parameterizations (in particular broken-wave celerity expression) in both time-averaged wave models and time-dependent Boussinesq-type models. We also derive a new wave set-up equation which provides a simple and explicit relation between wave set-up and energy dissipation. Finally, we compare numerical simulations of both, the NSW model and the simplified one-way model, with spilling wave breaking experiments and we find a good agreement.  相似文献   

Drag Coefficient,Dynamic Roughness and Reference Wind Speed     

Paul?A.?HwangEmail author 《Journal of Oceanography》2005,61(3):399-413

Surface waves are the roughness element of the ocean surface. The parameterization of the drag coefficient of the ocean surface is simplified by referencing to wind speed at an elevation proportional to the characteristic wavelength. The dynamic roughness is analytically related to the drag coefficient. Under the assumption of fetch limited wave growth condition, various empirical functions of the dynamic roughness can be converted to equivalent expressions for comparison. For datasets covering a wide range of the dimensionless frequency (inverse wave age), it is important to account for the variable rate of wave development at different wave ages. As a result, the dependence of the Charnock parameter on wave age is nonmonotonic. Finally, the analysis presented here suggests that the significant wave steepness is a sensitive property of the ocean surface and a single variable normalization of the dynamic roughness using a wavelength or wave height parameter actually produces more robust functions than bi-variable normalizations using wave height and wave slope.  相似文献   

Long-Period Distribution of Wave Heights and Its Application     

Weng Keqin Associate Professor  Hydraulic  Hydro-Power Engineering Dept.  Tsinghua University  Beijing 《中国海洋工程》1995,(2)

This paper reveals that the long-period statistic distribution of the characteristic heights of deep-water waves assumes the lognormal distribution. Thereafter, the largest wave-height which may occur in the service life of coastal structures is derived in this paper.  相似文献   

南麂站定常波风浪波高与风速的经验关系     

黄树生 《海洋学研究》1994,12(4):1-8

本文以南麂海洋站１９８３～１９９０年风、浪的实测资料为依据，建立了南麂海城春、夏、秋、冬季定常波风浪波高与风速的经验关系式。检验结果表明，曲线回归显著，计算值与实测值吻合良好。文中还对偏ＮＮＥ向和偏ＳＳＷ向计算波高随风速增大的快慢，同一方向在同一风速作用下计算波高的季节变化及其机理作了初浅的讨论。  相似文献   

Action of Cnoidal Waves on Vertical Walls     

Qiu Dahong  Zang Jun  Jia Ying Academician of the Chinese Academy of Sciences  Professor  the State Key Laboratory of Coastal  Offshore Engineering 《中国海洋工程》1996,(2)

Based on the 2nd order cnoidal wave theory, the characters of shallow water standing waves and their action on vertical walls are studied in this paper. The theoretical expressions of the wave surface elevation in front of and the wave pressure on the vertical wall are obtained. In order to verify the theoretical results, model tests were made in the State Key Laboratory of Coastal and Offshore Engineering at DUT. For the wave surface elevation in front of the wall and the wave forces on the wall at the moment when the wave surface at the wall surface goes down to the bottom of the wave trough, the calculated results coincide quite well with the experimental results. For the wave forces on the wall at the moment when the wave surface at the wall surface goes up to the top of the wave crest, the theoretical expressions are modified by the experimental results. For the convenience of practical use, calculations are made for the wave conditions which usually occur in enginering practice by use of the inves  相似文献